pRSET C
载体编号：P0084
载体名称：pRSET C
载体抗性：Ampicillin
载体大小：2900bp
载体宿主：E.coli
载体特点：
The pRSET vectors are pUC-derived expression vectors designed for high-level protein expression and purification from cloned genes in E. coli. High levels of expression of DNA sequences cloned into the pRSET vectors are made possible by the presence of the T7 promoter. In addition, DNA inserts are positioned downstream and in frame with a sequence that encodes an N-terminal fusion peptide. This sequence includes an ATG translation initiation codon, a polyhistidine tag that functions as a metal binding domain in the translated protein, a transcript stabilizing sequence from gene 10 of phage T7, the Xpress epitope, and the enterokinase cleavage recognition sequence.
The metal binding domain of the fusion peptide allows simple purification of recombinant proteins by Immobilized Metal Affinity Chromatography with Invitrogenís ProBond resin (available in bulk, see page v). The enterokinase cleavage recognition site in the fusion peptide located between the metal binding domain and the recombinant protein allows for subsequent removal of this N-terminal fusion peptide from the purified recombinant protein.
网站链接：http://www.bio.indiana.edu/~chenlab/potocols/prset_man.pdf
此质粒由“molecular12 ”共享，特此感谢！
