pET-23a(+)
载体编号：P0083
载体名称：pET-23a(+)
载体抗性：Ampicillin
载体大小：3666bp
载体宿主：E.coli
载体特点：
The pET-23a-d(+) vectors carry an N-terminal T7*Tag sequence plus an optional C-terminal His*Tag sequence. These vectors differ from pET-21a-d(+) by the“plain”T7 promoter instead of the T7lacpromoter and by the absence of the lacI gene. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA
that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer .
网站链接：http://www.merckbiosciences.com/docs/docs/PROT/TB051.pdf
此质粒由“molecular12 ”酷友共享，特此感谢！
