载体编号：P0078 载体名称：pGEM-3zf 载体抗性：Amp 载体大小：3197bp 载体宿主：E.coli 载体特点：The pGEM -3Zf(+) Vector is a derivative of the pGEM -3Z Vector and contains the origin of replication of the filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription and as a template for the production of circular ssDNA. The pGEM -3Zf(+) Vector contains SP6 and T7 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase (1). Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. For induction of ssDNA, bacterial cells with the F′episome (e.g., JM109, XL-1 Blue, DH5α.) containing pGEM -3Zf(+) Vector recombinants are infected with an appropriate helper phage. The plasmid then enters the f1 replication mode, and the resulting ssDNA is exported from the cell as an encapsidated virus-like particle. The sequence of the ssDNA rescued upon infection with helper phage is complementary to the sequence shown in Figure 1. The exported ssDNA can be used for mutagenesis in vitro or can be sequenced using the T7 Promoter Primer or pUC/M13 Forward Primer. 网站链接：http://www.promega.com/tbs/tb086/tb086.html 此资源由“liyuntao023”酷友共享，特此感谢！ [ 本帖最后由 冷酷到底 于 07-9-8 20:31 编辑 ]

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