酶切位点（Dnaman软件分析）
Restriction analysis on AF234299（pCAMBIA1303）
Methylation: dam-No dcm-No
Screened with 117 enzymes, 372 sites found
AatII GACGT/C 1: 10464
Acc65I G/GTACC 1: 11560
AccIII T/CCGGA 4: 9589, 9725, 10262, 12026
AclI AA/CGTT 1: 4906
AflII C/TTAAG 2: 2198, 2624
AgeI A/CCGGT 2: 6475, 10145
AhaIII TTT/AAA 6: 2931, 6541, 8198, 8227, 8594, 8754
Alw44I G/TGCAC 6: 950, 1275, 7008, 7506, 9901, 10203
AlwNI CAGNNN/CTG 5: 859, 2504, 4721, 4789, 7608
ApaBI GCANNNNN/TGC 5: 5437, 7017, 9036, 9910, 10186
ApaLI G/TGCAC 6: 950, 1275, 7008, 7506, 9901, 10203
Asp718I G/GTACC 1: 11560
AsuII TT/CGAA 2: 1110, 2447
BalI TGG/CCA 7: 638, 1996, 3364, 4912, 5179, 5646, 6592
BamHI G/GATCC 1: 11569
BbeI GGCGC/C 1: 6770
BbvII GAAGACNN/ 12: 2346, 10683, 11010, 11991, 12216, 3062, 8230, 10686, 10911,
11013, 11238, 12219
BclI T/GATCA 3: 106, 2362, 8909
BglI GCCNNNN/NGGC 10: 3236, 3622, 3856, 4044, 4056, 4065, 4918, 5331, 5643, 11754
BglII A/GATCT 1: 7
Bpu1102I GC/TNAGC 1: 8302
Bsc91I GAAGACNN/ 12: 2346, 10683, 11010, 11991, 12216, 3062, 8230, 10686, 10911,
11013, 11238, 12219
BsiI C/TCGTG 6: 7365, 10715, 11042, 9904, 10383, 12187
BsmI GAATGCN/ 6: 5153, 11765, 74, 5830, 7916, 8810
Bsp1407I T/GTACA 2: 1137, 1563
BspHI T/CATGA 2: 901, 8452
BspMI ACCTGCNNNN/ 6: 1349, 5835, 6229, 11594, 619, 10170
BspMII T/CCGGA 4: 9589, 9725, 10262, 12026
BssHII G/CGCGC 2: 2795, 2819
BstD102I GAG/CGG 6: 3605, 7125, 11504, 6691, 9738, 10096
BstEII G/GTNACC 1: 2573
BstXI CCANNNNN/NTGG 1: 11305
Bsu36I CC/TNAGG 1: 2091
ClaI AT/CGAT 2: 2208, 6061
Csp45I TT/CGAA 2: 1110, 2447
CspI CG/GWCCG 1: 10076
CvnI CC/TNAGG 1: 2091
DraI TTT/AAA 6: 2931, 6541, 8198, 8227, 8594, 8754
DraIII CACNNN/GTG 3: 2563, 9909, 10202
DrdI GACNNNN/NNGTC 9: 2180, 2477, 6887, 7300, 9604, 9985, 10960, 11287, 11948
EagI C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,
5738, 5885, 5958, 6074, 6679, 9543, 10113, 10278
Ecl136II GAG/CTC 1: 11556
Eco31I GGTCTCN/ 2: 6384, 2463
Eco52I C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,
5738, 5885, 5958, 6074, 6679, 9543, 10113, 10278
Eco56I G/CCGGC 16: 3301, 3475, 3525, 3529, 3556, 4055, 4413, 4730, 4870, 4959,
5205, 5426, 5633, 5637, 5782, 6763
Eco57I CTGAAGNNNNNNNNNNNNNNNN/ 6: 1038, 2175, 4093, 5002, 7740, 11789
Eco72I CAC/GTG 2: 2150, 2563
EcoICRI GAG/CTC 1: 11556
EcoNI CCTNN/NNNAGG 1: 6294
EcoRI G/AATTC 1: 11548
EcoRV GAT/ATC 5: 574, 805, 8029, 10653, 12253
EheI GGC/GCC 1: 6768
EspI GC/TNAGC 1: 8302
FseI GGCCGG/CC 3: 3533, 4059, 5637
HindIII A/AGCTT 1: 11599
KpnI GGTAC/C 1: 11564
MfeI C/AATTG 3: 147, 2385, 11997
Mlu113I CC/GCGG 1: 8904
MluI A/CGCGT 3: 612, 1310, 1328
MscI TGG/CCA 7: 638, 1996, 3364, 4912, 5179, 5646, 6592
MstI TGC/GCA 2: 6198, 11744
MstII CC/TNAGG 1: 2091
NaeI GCC/GGC 16: 3303, 3477, 3527, 3531, 3558, 4057, 4415, 4732, 4872, 4961,
5207, 5428, 5635, 5639, 5784, 6765
NarI GG/CGCC 1: 6767
NdeI CA/TATG 4: 2055, 4526, 7015, 10035
NheI G/CTAGC 2: 2540, 5981
NotI GC/GGCCGC 3: 3857, 5389, 6679
NruI TCG/CGA 2: 90, 1734
NsiI ATGCA/T 2: 2715, 7918
PflMI CCANNNN/NTGG 3: 2245, 4789, 5653
PinAI A/CCGGT 2: 6475, 10145
PmaCI CAC/GTG 2: 2150, 2563
PstI CTGCA/G 1: 11591
PvuI CGAT/CG 4: 2597, 9438, 10125, 11725
PvuII CAG/CTG 4: 1090, 2501, 11372, 11694
RleAI CCCACANNNNNNNNNNNN/ 5: 2282, 6787, 59, 296, 5643
SacI GAGCT/C 1: 11558
SacII CCGC/GG 1: 8906
SalI G/TCGAC 1: 11581
SapI GCTCTTCN/ 2: 7076, 6601
SauI CC/TNAGG 1: 2091
ScaI AGT/ACT 3: 3009, 9263, 9517
SciI CTC/GAG 2: 9426, 10520
SfiI GGCCNNNN/NGGCC 5: 3622, 4056, 4065, 5331, 5643
SgrAI CR/CCGGYG 1: 6763
SmaI CCC/GGG 2: 10503, 11566
SnaBI TAC/GTA 1: 401
SpeI A/CTAGT 1: 14
SphI GCATG/C 2: 2978, 11597
SplI C/GTACG 1: 5865
SpoI TCG/CGA 2: 90, 1734
SspI AAT/ATT 2: 1286, 7956
SstI GAGCT/C 1: 11558
SstII CCGC/GG 1: 8906
SunI C/GTACG 1: 5865
Tth111I GACN/NNGTC 4: 6939, 9066, 9982, 10426
VspI AT/TAAT 4: 965, 9238, 11364, 11423
XbaI T/CTAGA 1: 11575
XcmI CCANNNNN/NNNNTGG 4: 2567, 3385, 4540, 8558
XhoI C/TCGAG 2: 9424, 10518
XmaI C/CCGGG 2: 10501, 11564
XmaIII C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,
5738, 5885, 5958, 6074, 6679, 9543, 10113, 10278
XmnI GAANN/NNTTC 5: 9239, 10693, 11020, 11918, 12213
XorII CGAT/CG 4: 2597, 9438, 10125, 11725
List by Site Order
7 BglII 3527 Eco52I 5885 EagI 9909 DraIII
14 SpeI 3529 Eco56I 5885 Eco52I 9910 ApaBI
59 RleAI 3531 NaeI 5885 XmaIII 9982 Tth111I
74 BsmI 3531 XmaIII 5958 XmaIII 9985 DrdI
90 SpoI 3531 Eco52I 5958 EagI 10035 NdeI
90 NruI 3531 EagI 5958 Eco52I 10076 CspI
106 BclI 3533 FseI 5981 NheI 10096 BstD102I
147 MfeI 3556 Eco56I 6061 ClaI 10113 EagI
296 RleAI 3558 NaeI 6074 XmaIII 10113 XmaIII
401 SnaBI 3605 BstD102I 6074 Eco52I 10113 Eco52I
574 EcoRV 3622 SfiI 6074 EagI 10125 XorII
612 MluI 3622 BglI 6198 MstI 10125 PvuI
619 BspMI 3856 BglI 6229 BspMI 10145 PinAI
638 BalI 3857 XmaIII 6294 EcoNI 10145 AgeI
638 MscI 3857 Eco52I 6384 Eco31I 10170 BspMI
805 EcoRV 3857 NotI 6475 AgeI 10186 ApaBI
859 AlwNI 3857 EagI 6475 PinAI 10202 DraIII
901 BspHI 3961 EagI 6541 AhaIII 10203 ApaLI
950 Alw44I 3961 XmaIII 6541 DraI 10203 Alw44I
950 ApaLI 3961 Eco52I 6592 MscI 10262 AccIII
965 VspI 4044 BglI 6592 BalI 10262 BspMII
1038 Eco57I 4055 Eco56I 6601 SapI 10278 Eco52I
1090 PvuII 4056 BglI 6679 EagI 10278 EagI
1110 Csp45I 4056 SfiI 6679 NotI 10278 XmaIII
1110 AsuII 4057 NaeI 6679 Eco52I 10383 BsiI
1137 Bsp1407I 4059 FseI 6679 XmaIII 10426 Tth111I
1275 ApaLI 4065 BglI 6691 BstD102I 10464 AatII
1275 Alw44I 4065 SfiI 6763 Eco56I 10501 XmaI
1286 SspI 4093 Eco57I 6763 SgrAI 10503 SmaI
1310 MluI 4335 XmaIII 6765 NaeI 10518 XhoI
1328 MluI 4335 Eco52I 6767 NarI 10520 SciI
1349 BspMI 4335 EagI 6768 EheI 10653 EcoRV
1563 Bsp1407I 4411 EagI 6770 BbeI 10683 Bsc91I
1734 SpoI 4411 XmaIII 6787 RleAI 10683 BbvII
1734 NruI 4411 Eco52I 6887 DrdI 10686 BbvII
1996 MscI 4413 Eco56I 6939 Tth111I 10686 Bsc91I
1996 BalI 4415 NaeI 7008 Alw44I 10693 XmnI
2055 NdeI 4526 NdeI 7008 ApaLI 10715 BsiI
2091 CvnI 4540 XcmI 7015 NdeI 10911 Bsc91I
2091 SauI 4721 AlwNI 7017 ApaBI 10911 BbvII
2091 Bsu36I 4730 Eco56I 7076 SapI 10960 DrdI
2091 MstII 4732 NaeI 7125 BstD102I 11010 Bsc91I
2150 Eco72I 4789 AlwNI 7300 DrdI 11010 BbvII
2150 PmaCI 4789 PflMI 7365 BsiI 11013 BbvII
2175 Eco57I 4870 Eco56I 7506 Alw44I 11013 Bsc91I
2180 DrdI 4872 EagI 7506 ApaLI 11020 XmnI
2198 AflII 4872 Eco52I 7608 AlwNI 11042 BsiI
2208 ClaI 4872 XmaIII 7740 Eco57I 11238 Bsc91I
2245 PflMI 4872 NaeI 7916 BsmI 11238 BbvII
2282 RleAI 4906 AclI 7918 NsiI 11287 DrdI
2346 BbvII 4912 MscI 7956 SspI 11305 BstXI
2346 Bsc91I 4912 BalI 8029 EcoRV 11364 VspI
2362 BclI 4918 BglI 8198 AhaIII 11372 PvuII
2385 MfeI 4959 Eco56I 8198 DraI 11423 VspI
2447 AsuII 4961 NaeI 8227 DraI 11504 BstD102I
2447 Csp45I 5002 Eco57I 8227 AhaIII 11548 EcoRI
2463 Eco31I 5153 BsmI 8230 Bsc91I 11556 Ecl136II
2477 DrdI 5179 MscI 8230 BbvII 11556 EcoICRI
2501 PvuII 5179 BalI 8302 Bpu1102I 11558 SacI
2504 AlwNI 5205 Eco56I 8302 EspI 11558 SstI
2540 NheI 5207 NaeI 8452 BspHI 11560 Asp718I
2563 DraIII 5331 SfiI 8558 XcmI 11560 Acc65I
2563 PmaCI 5331 BglI 8594 DraI 11564 XmaI
2563 Eco72I 5389 XmaIII 8594 AhaIII 11564 KpnI
2567 XcmI 5389 NotI 8754 DraI 11566 SmaI
2573 BstEII 5389 Eco52I 8754 AhaIII 11569 BamHI
2597 XorII 5389 EagI 8810 BsmI 11575 XbaI
2597 PvuI 5426 Eco56I 8904 Mlu113I 11581 SalI
2624 AflII 5428 NaeI 8906 SstII 11591 PstI
2715 NsiI 5437 ApaBI 8906 SacII 11594 BspMI
2795 BssHII 5633 Eco56I 8909 BclI 11597 SphI
2819 BssHII 5635 XmaIII 9036 ApaBI 11599 HindIII
2931 AhaIII 5635 Eco52I 9066 Tth111I 11694 PvuII
2931 DraI 5635 EagI 9238 VspI 11725 PvuI
2978 SphI 5635 NaeI 9239 XmnI 11725 XorII
3009 ScaI 5637 Eco56I 9263 ScaI 11744 MstI
3062 Bsc91I 5637 FseI 9424 XhoI 11754 BglI
3062 BbvII 5639 NaeI 9426 SciI 11765 BsmI
3236 BglI 5643 BglI 9438 PvuI 11789 Eco57I
3299 EagI 5643 RleAI 9438 XorII 11918 XmnI
3299 Eco52I 5643 SfiI 9517 ScaI 11948 DrdI
3299 XmaIII 5646 MscI 9543 EagI 11991 BbvII
3301 Eco56I 5646 BalI 9543 XmaIII 11991 Bsc91I
3303 NaeI 5653 PflMI 9543 Eco52I 11997 MfeI
3364 MscI 5738 XmaIII 9589 BspMII 12026 BspMII
3364 BalI 5738 EagI 9589 AccIII 12026 AccIII
3385 XcmI 5738 Eco52I 9604 DrdI 12187 BsiI
3475 Eco56I 5782 Eco56I 9725 AccIII 12213 XmnI
3477 NaeI 5784 NaeI 9725 BspMII 12216 BbvII
3525 Eco56I 5830 BsmI 9738 BstD102I 12216 Bsc91I
3527 NaeI 5835 BspMI 9901 Alw44I 12219 BbvII
3527 EagI 5865 SplI 9901 ApaLI 12219 Bsc91I
3527 XmaIII 5865 SunI 9904 BsiI 12253 EcoRV
Non Cut Enzymes
ApaI AscI AvrII Eam1105I Eco47III HpaI
I-PpoI NcoI PacI PmeI SrfI StuI
SwaI
