Name: pBluescript-LION
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General: phagemid ds-DNA 2943 BP
/ r# m# [; d$ ?! }. e* iFunction: cloning, sequencing
O4 ^! c4 Q1 i$ uPromotor: T3, T7
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Resistance: amp
9 Y$ s7 [3 @' A' a( {7 K- kOri: pUC
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Host: E.coli
: U; ?- M& b' e9 ^* f& `Supplier: -
) b8 X L7 j) B( JAccNo: -
6 X8 i7 a, L* }8 `8 gMiscellaneous: -
' e% G/ j7 Z( G; n; Y0 J1 @ q7 O* URelated to: pBluescript series
7 {+ n9 S1 O5 d4 NLink: -
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载体图谱如下：
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d6 Z w& q1 t) l3 G4 S; a载体序列如下：
* j6 b( q) W+ y8 m 1 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggaatt
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61 cggcgcgcct atcgataaat ccgatatcac tagttctaga gcggccgcca ccgcggtgga
' w W6 s$ H# j) i- Q$ ] 121 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
# f {. J3 Y5 u9 x7 ?/ L
181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
6 i. C9 \3 M$ e# i; s5 a5 P( ]
241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
; g3 Q ]! ~3 ?$ `" a% @6 i
301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
, D* F, O8 {6 i) W 361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
3 [) o a s% A5 X+ D( A( A 421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
N1 b, C- P$ @% H* o 481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
5 ]8 T# u& d# W
541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
* [ C1 z% F$ b) \& d+ \ 601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
3 z3 F: Q* a" y+ G 661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
- g9 `7 V8 C; |, B% o
721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
3 ?) I8 e8 H! M. ~
781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
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841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
- c/ p) C5 m& ^& ~8 U- I! K 901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
/ ?3 |5 p ^! Y! {7 \: f 961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
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1021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
9 ^8 ^$ X! l+ Y. A* f 1081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
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1141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
, d9 n" S2 x' T2 Z3 { 1201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5 j3 ^: s m" B* b' [/ W/ @" m 1261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
/ a3 b& S% B) G0 s U' f* @# ^: H- \ 1321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
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1381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
, X' i# p! D5 `. ` 1441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
1 U0 I8 I& p; z 1501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
5 Q, X, G8 s) ~ n 1561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
. T* M) m- K! H/ g7 H3 E 1621 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
0 Q2 | f" l+ n4 C p& g+ ]5 y 1681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
4 T0 Z% m+ V& q% q! s: `
1741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
& d* @/ m) {/ l. P 1801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
( k& a% u# k. W% ~1 f8 F# v 1861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
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1921 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
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1981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
# o: @- u' y0 S% K( _ 2041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
3 L2 p& r; l S. c: S C 2101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
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2161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
4 X+ F/ }6 D/ A9 ~ 2221 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
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2281 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
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2341 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
2 ]; Q: \/ ^# B 2401 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
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2461 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
7 h6 z3 }) e6 P/ B6 Y 2521 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
) D6 o1 h; m; p& D 2581 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
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2641 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
s$ s" u7 R: _# m
2701 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
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2761 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
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2821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
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2881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
# f; }$ a- ]+ z w. K7 T. b 2941 tga
