Name: pBluescript-FL
, \) j, K' X9 q. K
General: phagemid ds-DNA 2985 BP
8 l( g; T1 ~' i9 W- dFunction: cloning, sequencing
[% _3 k3 O, Q8 m3 lPromotor: T3, T7
+ D5 o) I* Z8 \+ A- {
Resistance: amp
' W# H: _- C8 z R7 E
Ori: pUC
7 H" c* O1 m) Y9 R' O! OHost: E.coli
7 V! d* `: A8 N. I" `
Supplier: -
* |" D; S2 t E- dAccNo: -
; y z5 ^- d$ ?. H1 p7 vMiscellaneous: -
' k: b P# C+ M+ J4 i* YRelated to: pBluescript series
& w! R1 f$ d* `- g# b3 \- mLink: -
& B9 ^ O+ B# Y! o
Z% E( B$ h& O g载体图谱如下：
* m& V& |% D- c+ x+ f W+ s
& R {" h: @" C, f8 h
" l3 _/ ]+ |3 w# W }
# E- y& [- z2 q0 g, b4 p( g# z! X8 C; A+ k
载体序列如下：
: v m5 k0 t8 d/ Y
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
# j3 [7 `4 z B+ v! P* e# @" ~ 61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
7 I2 Y, ~" ~/ \3 N& L/ x
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
7 t- K7 l) v" r7 ]2 j) S& k$ q n
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
8 N _4 m- C8 z 241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
( S9 v1 y3 F: w8 p2 m, r
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
& `# [. F6 _$ M, V 361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
+ c/ T& n0 n; Z9 E: }6 N6 L& X 421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
9 Q- a1 S9 a, G t9 k( r
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
$ A+ z; X* m' d& R, s& ~0 ~ 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
. X$ a# e; P w2 S
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
& J& M( Q( h/ k- p* y) e 661 ccgcggtggc ggccgctcta gaactagtgg atccattgtt tggccttgtt ggccatcatt
. X' B5 Y/ s, g
721 tgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
4 {$ C5 y. k2 I+ I
781 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
# f$ a$ \8 p2 I9 {0 q- n9 b9 g
841 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
* f$ m4 @8 j' T* H% i' w
901 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
0 I$ U3 r, b- Y K1 D6 J* `9 T9 d 961 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
' v6 l$ H: \4 E- W# x) x" Z
1021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
0 i6 P4 `& h9 [7 W5 Q: r
1081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
$ X& a1 q- f+ e6 \8 V 1141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
3 @) |2 y1 M- w: b Q& E! l Z8 G 1201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
; J! S8 j" z7 I+ K 1261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
0 W9 I+ N4 W- Y* j/ r 1321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
+ B0 E; x, f# } 1381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
: y* u2 J! d& a. G- V" E 1441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
8 d0 E2 N0 t6 \6 B; I
1501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
3 I+ n3 G$ D# `
1561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
; s# t6 {) p- d0 o% R) D 1621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
, W+ S! d9 u8 D4 b 1681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
: v( A" U, R D# l; f
1741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
$ I# v% s$ M% ~: i- Q% y
1801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
$ a$ F- ]+ v# l 1861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
* @/ B d: [& n) i& G$ B, H 1921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
' f1 |6 n! m1 H2 m. p. P' b 1981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
) d* ~9 Q2 m! u6 s9 v) v& Q 2041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
. t; v8 j2 U. j+ f; p
2101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
a+ q4 R: t0 z m' n- W. ]$ J
2161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
0 M/ w) [4 r7 n# U* L @. o% ~, U
2221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
% l( Y, k4 w+ @* u6 [7 B8 ^ 2281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
" {6 i. q, H) _+ p9 ]+ z, ^! k
2341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
: \! |5 z5 w% i- W
2401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
; u w' [2 U% C4 t
2461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
6 \( g8 @ b2 b% Z3 J' } 2521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
% z, F# D: T% M" O! N7 ?
2581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
0 y( {* f% F2 ] B( R* V
2641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
6 `; d$ \+ x4 a( x9 n% v) W
2701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
. }% K0 e+ N, X, }; ]+ ?( d- H 2761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
' B! r' C: f, P. D' ]6 M# a. S 2821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
8 Q% c8 g- q+ j0 g- C8 b5 z 2881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
/ q( v3 x' V; d. b 2941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
