Name: pBluescript SK+
* |' `3 c+ O# ]7 L6 xGeneral: phagemid ds-DNA 2958 BP
& @8 F" _' t# U# R
Function: cloning, sequencing
) d* I: x0 w2 N) R9 z+ l2 rPromotor: T3, T7
* f+ l1 T7 x8 N8 Y% UResistance: amp
' M( ?1 d: y; w% N2 c6 @
Ori: pUC
) K* A' L. k8 Y, M* I
Host: E.coli
7 K* _) |- w) n6 n
Supplier: Stratagene, discontinued
6 X1 s& M7 C3 K
AccNo: X52325
% |& Q) K4 N& u" ^: h0 _5 k) |
Miscellaneous: -
, S4 }1 o0 G7 O# JRelated to: pBluescript series
) U, P5 [8 [* x8 J% l6 {% w- y Q
Link:
www.stratagene.com ..
, Q1 |% A; O! H/ _" k" G, d
) j1 y: H* X! u. L3 f2 ~$ `' _载体图谱如下：
7 r) F( X4 p8 E9 w$ B1 a2 y; \& s4 J+ r
: W5 l7 |+ {/ A9 q* r载体序列如下：
1 ]1 v% w6 Z/ x2 y 1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
- ?3 h( X& U7 X9 U, o
61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
1 K. j. y7 b/ g$ C. t' V
121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
; w% P B* B! D1 t# ?: I 181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
; z0 R: ~& }/ A 241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
- n" {0 w9 i- D
301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
! J f) {! i. B( L9 ?) n, T
361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
- B3 W; ?8 P) D8 A0 J- Q3 W 421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
" x; x0 E# [% z/ p8 O
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
; X4 r; U! H! O, i! H# D 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
' t* f2 z4 N9 l2 Q2 h 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
, ?* t" t$ l3 U) o5 K; ?' o
661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
# `# j: S& \+ c' f3 f( j+ s9 M
721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
0 ?, @5 @4 L; W- C- a6 d7 \( Y 781 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
% l4 D8 C+ ^3 K/ I; i 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
6 h" U1 Y# n8 i2 X 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
, W T/ v4 ]+ B% {/ n/ z. j, O
961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
0 I c& e: G! [3 c+ U0 i: ] 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
( B, z' B; \" m1 m+ i0 x" C 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
[% R* S) f* A+ D: U 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
6 b" f6 c* k# Z' \ y) u 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
. V5 y* L0 [7 b. f; D! Y% w 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
! m5 ]- n* g! M1 e 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
+ E7 N8 z F' |1 c( S8 c8 w6 @. H# R
1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2 G) @( J$ }# |* F
1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
$ }# y M- G; l5 D
1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
( z' T( `( K, K' L1 Y4 b; I3 r( R7 u
1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
- i/ i3 I4 g. ?1 O" u! W. @
1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
5 t3 G9 ~: T5 h0 ]5 t" {) h+ q 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3 ~8 G" I( V8 _: g! g5 r- A
1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
, N+ @" D% w, P% [ 1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
0 T) i9 x/ Y M- B! A) V$ J4 R
1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
9 t+ U& z- j& Q y
1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
& u, C" j9 l0 v8 }( ^" e
1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
& P" T* J0 v* v, d
2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
: {6 x7 r1 K& a1 i+ \ 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
! V5 [$ E1 m/ A& t. [/ T, P 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
1 z3 G C: @* Y v2 H( u g
2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
# k8 N( y' \5 P 2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
* t) w/ B [0 D& r7 i% y
2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
& Y5 z0 r, _1 ~8 i8 `; c V: [3 i
2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
8 X. m0 A! H( p9 S
2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
! l) F8 `9 y; c/ k( _- F) q. n& p
2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
- n2 z1 N h+ }1 i) Q; G
2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
# T* o' Z! C. }5 g- ~& T* {7 \ 2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3 o# v! k5 Y- e$ a( B
2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
# g+ v+ B' j; g8 u 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
- P+ r; A* `6 q! [9 O1 f [4 q& D 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
# C" R& W+ ]# ~% T' L9 W* A# B
2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
* H% w8 G: c/ d) K 2941 catttccccg aaaagtgc
