Name: pBluescript KS+
5 A2 K; ~) ^9 |% _+ rGeneral: phagemid ds-DNA 2958 BP
: u( N& O @0 k/ OFunction: cloning, sequencing
( Q9 S& m M+ HPromotor: T3, T7
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Resistance: amp
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Ori: pUC
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Host: E.coli
# j$ L* D8 B& t, i9 c, PSupplier: Stratagene, discontinued
5 F! ~6 u. r, u+ Z6 aAccNo: X52331
/ K3 d3 H [1 K l2 K1 FMiscellaneous: -
! B/ b4 H1 c$ T7 B* ?3 ], D% vRelated to: pBluescript series
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Link:
www.stratagene.com ..
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载体图谱如下：
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载体序列如下：
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1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
: b0 L% v6 O5 a& X3 d9 i 61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
3 ?4 }0 c7 k" w$ @; V; H% W
121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
2 H3 E; A. c5 g
181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
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241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
6 k3 F) P2 U) J8 n5 K* L& B 301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
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361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
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421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
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481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
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541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
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601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
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661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
9 W+ e4 O% ]: S5 Q1 x4 V' R 721 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
: o1 f7 l! k1 l: n ?+ T! t8 i$ O 781 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
5 c* ~6 ~: P4 O, }- k& ~ 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
5 [$ h( ?( E0 q+ M 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
N; q6 |: w8 z. z! |+ R
961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
! g4 `" h& d. K$ @& |. b 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
% K/ k! C' _9 p0 x5 m6 S 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
# J- p7 v* Z' ?8 C 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
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1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
: D6 k6 ~8 r2 e' D. L" D 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
H# C1 ^9 k3 Z( L 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
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1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
+ ]( m( b- m8 n8 N2 j$ H 1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
' ~$ U6 q1 L6 V 1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
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1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7 `: q4 j- x! u* f 1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
# o9 L) k3 `" r( ]8 S% } @" n/ x3 N 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
; ^9 {3 S! w$ y5 }# i, m 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
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1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
- |$ I$ ]- R# e2 J 1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4 J# i; t6 W& V" y/ O: d3 z( A' m 1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
# V0 X# q) u$ A1 I8 G) }/ e, {- S0 O 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
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2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
9 R1 \4 G# p7 i0 v) \ 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
4 `$ P8 A( d5 H" x& r) x6 X 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
9 I% ^% Z: ?+ H" ? 2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
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2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
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2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
1 n# X3 L u( \8 v: m2 S8 u 2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
: }: \8 A1 v' z# p 2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
1 T) I( I: x+ U1 e$ E 2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
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2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
+ D Y" t( T+ L2 O 2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
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2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
6 e4 r* c) A& s& E# N, p4 H( S7 e7 f 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
- B5 ]$ F! x0 P+ E# u9 H 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
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2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
- a. x a; ]* o5 @ 2941 catttccccg aaaagtgc
