Name: pBluescript KS-
3 P' X9 D8 p p) F& N8 c3 Z$ i+ sGeneral: phagemid ds-DNA 2958 BP
8 G) Z: |( p8 s2 [5 i. u# pFunction: cloning, sequencing
& Q2 W% @: U8 N5 ^5 O _; q5 H, aPromotor: T3, T7
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Resistance: amp
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Ori: pUC
1 {* d$ l) m* }2 tHost: E.coli
; r* M' S2 | K3 aSupplier: Stratagene, discontinued
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AccNo: X52326
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Miscellaneous: -
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Related to: pBluescript series
V+ w* L! U- C0 {4 LLink:
www.stratagene.com ..
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6 Z( h* P/ j M9 @& p$ Z载体图谱如下：
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载体序列如下：
& M6 h4 }& a, q, B
1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
; T# l6 ^& n$ ?
61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
9 C( X* @- ^4 E( P$ [ g6 t 121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
/ E, o3 z! ^* j$ K# c: b& ?. `
181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
# M" i2 w" ]# O2 t! M3 [
241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
& A7 J0 d a1 d! r" A7 Q0 j" B1 Y7 Q 301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
; ]6 \5 i) b' N4 F
361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
4 S3 e9 m1 r0 e1 Z2 w& D, Z g
421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
4 L1 ~9 H; O3 W: x, h) e
481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
5 T, e. J% k: U5 n. C, ^
541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
- b" m9 q6 d. p' h% D
601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
3 g, Y/ v3 l* ]. M' k" z& D
661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
6 I" w" K* F+ \0 {8 s
721 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
6 D( _" f: @1 z( R4 h; ?
781 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
+ B5 r3 [2 C) p. J& e, v$ Q, @% S 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
`+ f4 e$ ]0 T; e: X+ z4 E 901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
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961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
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1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
% R- T; F2 I1 C, {" B- G
1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
. C" S: F; P6 [ 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
1 ^" L0 H$ p, z0 c% q 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
- C9 Q; C3 x! q6 k9 B
1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
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1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
7 F0 j! l. u) B7 ?# P- n! m7 P2 Y% n
1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
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1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
, v3 }) ~! H {7 ~- I7 K& j8 G
1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
( ~$ _% ]8 o5 Q2 S0 L- M
1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
' }0 n. o+ p: D8 {# ?2 ~
1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
6 P- `, {1 {3 f# Q& r3 \* u
1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
! G, D' ~/ {* p( g9 \/ S9 Q 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
- a5 h" O" D% R" k0 }2 T' B 1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
" c2 ~) B; K9 H 1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
, g+ f$ w: @+ x7 u: E+ A5 }
1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
+ T4 X% j# V& s" v0 s+ o& r" ^5 ], a 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
1 E# ^4 I6 S: X 2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
* H! U; j# O! V 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
( H! H9 b2 v1 w5 K 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
; x7 ~ a0 q, `6 v 2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8 n W- ]9 l4 Y. X: h5 ^8 @) H 2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
" h- K& L* H, _1 s) ^2 Z 2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
+ w" I! b: x" Y2 s d 2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
" A) d# a7 ?% b4 v: O
2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
5 V+ |0 n; n0 w% Q; }8 ~ 2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
$ J5 i, ]6 R. r- K/ \& T' F 2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
: {8 Y" c: _& N# u9 H8 u% k5 B: h* m
2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
9 M% Z+ K9 f* E; N1 E
2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
' u8 \; s8 F$ n z5 P R- g 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
/ @- n6 a0 n! H+ N3 X5 ~
2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
8 q) ]8 H& P+ Q* v) h 2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
* m. `# H! ]0 J: L 2941 catttccccg aaaagtgc
