Name: pBluescript II SK+
6 r8 Z+ U8 G) lGeneral: phagemid ds-DNA 2961 BP
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Function: cloning, sequencing
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Promotor: T3, T7
- w: V6 u8 B7 M1 ?. i% u- s k7 MResistance: amp
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Ori: pUC
+ I) z3 Q* B; g! Y+ B9 n" mHost: E.coli
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Supplier: Stratagene
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AccNo: -
2 q3 i; E9 X7 z1 c* V& [. fMiscellaneous: -
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Related to: pBluescript series
- N) c; F. X# H) k$ \7 X% rLink:
www.stratagene.com ..
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2 K7 A# s) H# i+ q$ \2 L载体图谱如下：
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0 J, m4 ~% m* u# i: k载体序列如下：
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1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
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61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
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121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
$ J$ [) e" O$ M4 J 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
, j1 C' A0 [: U, f 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
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301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
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361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
" k9 i# s8 R1 d0 [ 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
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481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
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541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
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601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
/ H5 R; ?0 ]$ J9 u# l& n) w: l& O 661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
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721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
9 N7 g- p" `; u6 W 781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
3 O( z# K. \0 o4 ~ 841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
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901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
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961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
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1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
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1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
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1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
& b7 x c# r! m6 y3 u7 O 1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
( b9 i- v% \2 c* t0 u* @ 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2 J T: |1 {& F) q2 x 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
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1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
$ K# V# Y0 K6 Z" b3 X* W$ g6 K 1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
6 [5 ~; \2 a; U$ }. f 1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
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1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
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1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
# X a' O/ N B/ ?% o 1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
: e$ [% k! J1 R! y- J7 S6 B! D" G; C 1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
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1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
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1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
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1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
" i, a+ F* O+ k6 R 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
* S/ ~) V: Y4 T! y 2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
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2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
& q9 l% D- h; M! s 2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
g7 M, k9 T* o2 n5 u 2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
/ C8 j7 A' K+ l$ y& A7 O 2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
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2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
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2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
% n' r6 \! _4 m* ~6 @ 2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
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2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
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2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
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2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
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2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
, X& U/ U8 u+ {6 `. f 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
8 L, R) b: c! D 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
* T1 M7 n$ X1 z/ ] 2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
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2941 catttccccg aaaagtgcca c
