Name: pBluescript II SK-
! D* ~/ A2 }$ j: I) G) u3 tGeneral: phagemid ds-DNA 2961 BP
2 K9 J$ S/ k; n9 ~/ u S% W8 AFunction: cloning, sequencing
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Promotor: T3, T7
1 Z/ u, `4 y( b! s4 o5 lResistance: amp
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Ori: pUC
8 ^$ x* I6 i& k# U3 H" b( F8 NHost: E.coli
) B: E& Z1 `6 M+ D# wSupplier: Stratagene
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AccNo: -
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Miscellaneous: -
$ o2 F$ T, i) E" q! w8 PRelated to: pBluescript series
( W/ T; t: n+ \# j- @8 pLink:
www.stratagene.com ..
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( F7 s4 Y" \2 N. q/ m8 W6 i载体图谱如下：
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序列如下：
. d1 T% l" R) l* m
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
0 B; A* e* y. r9 p) m) T 61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
0 y7 P4 X4 h5 |' V 121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
* u- C+ [2 {5 ~& D9 N% ? 181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
9 F% j4 { P5 i/ w/ A8 f$ A0 r 241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
/ @5 N6 q; H" g3 J2 l" h 301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
! F" c$ d& E8 I. P2 v* n 361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
. I, L; u6 X7 I, {" L
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
& G/ q [. [- n( c 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
/ D3 S. ?" Q" d
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
& Z* C: Y1 j( n) @# W, M 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
& S! b. D! u F4 a6 |( i4 Z! m 661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
1 k' E$ e! f4 Q* }& A% f1 h( y+ U 721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
: V7 I' i5 f: J
781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
8 s5 U% C$ M4 J4 }* D
841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
: f: q+ \2 A x- W) Q! m
901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
+ d, ^" o( ~. w$ l0 {; f6 V
961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
9 }; Y* ]7 h7 J5 m4 O7 d, z 1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
( v1 Z4 P$ t6 a J/ F) P 1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
3 }# j8 ~8 \) K% R 1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
/ X3 n) l7 t) u6 |: u
1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
^" e# W+ u! y' H 1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
' f9 g! M. G$ T/ Z7 ` 1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
1 J( i* `1 u) v+ i 1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
, E. i/ ]4 Z& K. G2 c$ w. B$ V
1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
! p; }" D1 W* X. o, Z7 j( F
1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
8 r+ j( g1 _& T% _0 T5 X, A 1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
# X: N. r- V# \( i5 W
1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
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1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
I9 \9 k6 S. s: l" F: [, C |
1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
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1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4 p: H. W! J" D$ O3 X+ y5 ]5 d4 O
1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
T1 C( C& |$ ` ~$ L9 ?
1921 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
, D9 P0 n1 c9 [5 J2 a 1981 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
2 y# s& o2 G: H& `
2041 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
, P, Z/ a) S& l 2101 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
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2161 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
& b6 m( f' O2 H! y7 M$ A8 }
2221 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
0 M7 f& L+ Y; g* s! E9 Y
2281 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8 Y4 P( L. _2 y9 ^2 g8 I3 r8 R C 2341 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
# v( ?6 b. {4 e! b% e7 z0 q
2401 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
5 v2 w5 _! F+ w- N6 W
2461 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
C1 Q3 W. f7 X8 {+ l: C8 k 2521 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
% Z0 Z A: K; u6 l8 K' c( B# f 2581 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
& B! r- x) p* Q+ b% O5 S) s
2641 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
, H+ s* C6 L$ J3 q3 f- p 2701 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
9 \; r o$ X ?% x( `& ~. c6 e 2761 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
4 f7 v( L1 Y1 H- ^8 r/ ~ 2821 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
$ ~6 ]$ j$ I2 s& J 2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
0 h/ V6 ?4 K4 v' j$ ?! x3 L. w
2941 catttccccg aaaagtgcca c
