[质粒图谱] pCAMBIA-1281Z

pCAMBIA-1281Z质粒图谱及相关信息2 r* j+ c& A6 A8 C' `

; h$ ^+ A3 d' f* j图谱库ID：* j) n% x8 S$ s

! B2 m+ {, Z$ }; y/ O质粒名称：pCAMBIA-1281Z% P: J3 M' [$ w* e0 j4 F2 l/ j0 v

6 \  k. A8 o1 n( |, _( n载体类型：Bacterial
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报告基因：GUS  HIS-TAG
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启动子：CAMV35S     LAC
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pCAMBIA-1281Z图谱1：
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pCAMBIA-1281Z图谱2：
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pCAMBIA-1281Z序列：' z" P' J* B+ E9 t4 e
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LOCUS       AF234294               11378 bp    DNA     circular SYN 24-APR-2000
3 t- e% W% H# {; }, K1 K! t2 DDEFINITION  Binary vector pCAMBIA-1281Z, complete sequence.2 l$ C, ]2 W4 |$ D8 v  \
ACCESSION   AF234294; s$ j4 `! y* N. ?6 g4 Q( Z+ N3 v
VERSION     AF234294.1  GI:76380544 U9 F% z& U0 L. M& \& E# Y% B
KEYWORDS    .) N0 @& T& y. r1 K% t" u
SOURCE      Binary vector pCAMBIA-1281Z
- D6 V% n0 p& T  ORGANISM  Binary vector pCAMBIA-1281Z
( V$ a. \) g! X% L- ^$ K            other sequences; artificial sequences; vectors.
0 z, Z+ W! E- j9 a  G5 V* YREFERENCE   1  (sites)4 b8 a/ p; v. G$ Q  I7 |5 Z
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
- x$ `1 H# V, }  j! \  S  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors* r* J, _6 x+ I9 R! a
            for plant transformation! A: ~: }/ y; s& b; L! C9 W, c) X
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994): k8 D, y4 x2 ], e% g
   PUBMED   7919218( k; D9 L9 _1 p* p! d+ h
REFERENCE   2  (bases 1 to 11378)2 l( x7 E' c; Q
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,6 x6 u% H) U4 L; S9 u4 s7 C" m
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
. l& V- s$ j7 Z9 y2 Y9 V            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,3 S5 c# s9 x& h' ~/ {5 d9 {) a
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
! o% X3 e7 r. s" K  TITLE     A comprehensive set of modular vectors for advanced manipulations
7 S/ K$ N- X7 [8 A/ I            and efficient transformation of plants
2 B! n& ^9 k+ W! D3 T" j! b8 i  JOURNAL   Unpublished
( d" M7 H$ @+ m# a+ O. [8 P  REMARK    Full description of constructs
: R& j0 i1 W6 \REFERENCE   3  (bases 1 to 11378)( Y9 I4 }- q/ a2 _0 S9 \
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
1 t3 ?0 f5 R6 X/ I+ N            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
) J0 Y1 S- p, a$ t! l  l) g! Q            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
+ y) L* a! G7 e            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
7 ~" W" [! w+ b' p6 m. S7 q* u1 p$ I  TITLE     Direct Submission
* Y+ X* F0 P( d. w3 K7 j: `  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /, q3 B8 s- ]( Z
            GPO Box 3200, Canberra, ACT 2601, Australia3 m3 a$ \8 D$ ?+ k5 M0 D0 F1 |
FEATURES             Location/Qualifiers
" O  ?$ ]: @0 @" P- g     source          1..11378
* e" i& m' l/ Q$ p                     /organism="Binary vector pCAMBIA-1281Z"
6 Y9 z& P- ~' S2 p% M8 g! q                     /mol_type="other DNA"# ]. ]4 S! y; k0 p
                     /db_xref="taxon:118386"
! X9 G" T( K  B! j' u$ v7 r5 h5 L     CDS             join(2..16,207..2054)  i( B$ P; p  S+ z
                     /codon_start=1; k9 @; B& r  X8 l! r9 d
                     /product="GUSA, hexaHis tagged"$ A7 }( L# f% r5 [$ r
                     /protein_id="AAF65330.1") @1 I, k4 }5 Y4 e8 g. P  }, \
                     /db_xref="GI:7638057"& P& ^; o; k/ n* Y4 X
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE; c7 s9 B3 {1 t: d7 P3 t. C
                     SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
, }+ t) k/ Z0 f                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
! C. D' T1 ]% t; v1 `                     NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
3 ]$ y2 C5 M, Q8 {5 ?5 S                     DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR
- C: Z5 e( A9 `" M& g/ N1 C                     VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY
2 M# V* `5 r$ N0 b                     RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET) ^$ d% H) C# s2 P  N* C9 E
                     QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT, P$ {: Y0 u" R) g
                     CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
0 \4 Z) W" ?1 T2 c3 C; |                     IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ8 n! W6 z! E( ?; A" F+ V" ~
                     GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"' o, L4 i1 ~! S: s6 T0 n$ B
     exon            2..166 j9 n6 V* v$ f% ]$ h6 U* r
                     /note="synthetic first gus exon (modular)"
1 k# b) q7 b6 x- {8 ~4 d7 f     intron          17..206$ ~. q. U* t$ j: |- t
                     /note="Castorbean catalase intron; modified"0 m4 z" P( `" Y" D$ i
     exon            207..2054) U& K; ^3 V8 E# H- A7 U
                     /note="second gusA exon, His6"; v$ f/ Q' T% v$ N. `& v9 ~
     misc_feature    2086..2338
! U  E- G& \- p" Y7 [                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
2 |2 s/ k' r4 p+ u* m8 O     misc_feature    2376..2401
4 n% g. Q3 N. f; p7 A' H, d) X$ A                     /note="right border T-DNA repeat"
7 A/ r# b" ?: |4 a, O2 g     misc_feature    complement(3442..4442)
9 M2 |' I4 f: a& ~6 k; H                     /note="STA region from pVS1 plasmid"
9 R3 \' L7 _# m7 _     rep_origin      complement(5035..6035)/ |9 ^6 h! l- x& V$ `
                     /note="pVS1-REP; replication origin from pVS1"5 ]/ {2 a! ?8 t( |" J$ @. ?
     misc_feature    complement(6445..6705)
1 V7 u  `; C5 H7 _0 Y! T                     /note="bom site from pBR322"
, V0 F3 h7 l  f) k. a     rep_origin      complement(6845..7125)) Y- Q% P$ J: A/ l" {+ D- s( W- `
                     /note="pBR322 origin of replication"
4 V1 R3 \5 x' k; ~     CDS             complement(7600..8259)9 y: ^( [7 {4 E2 P) \
                     /note="cat (chloramphenicol resistance) gene from pBR325"
" ?9 h" {3 t2 P- g& g                     /codon_start=1
( s! e0 i: ?) r8 d                     /product="chloramphenicol acetyl transferase", J" X) B: ]4 c; p5 R
                     /protein_id="AAF65328.1"
' v: H# V9 f" N                     /db_xref="GI:7638055": O+ `, Z' G9 X. @; j% b
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF- k+ q( ^3 G# p0 I* {
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
1 \/ F$ T) h1 Z1 d3 o1 J                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
  F  ]- `, b) f. B( t" F- u                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
9 M- Q* F8 E- j) h8 k9 u7 ^                     A"
1 @0 b/ e' n/ `. U2 Q1 c     misc_feature    8787..8812/ z5 E4 t$ \3 ^2 I( B& G- X) q
                     /note="left border repeat from C58 T-DNA"2 p9 Z' @) E4 Q* j
     misc_feature    8879..9087- y5 E6 K% o/ F6 ^
                     /note="CaMV 3'UTR (polyA signal)"  S; z5 T8 F! g+ B0 E0 Y( J
     CDS             complement(9103..10128)) v9 l8 s# O1 p0 _: h7 g" ?" B
                     /note="hptII (hygromycin resistance) gene"' G5 g- D2 R2 D. p$ o( K3 ^
                     /codon_start=1" V& L6 H7 r4 G3 x& j. f
                     /product="hygromycin phosphotransferase"
' R" G" O+ M3 a% A                     /protein_id="AAF65329.1"
8 h( ^1 E/ l; z* H0 P                     /db_xref="GI:7638056"% h% I) y" X# G9 x9 P) J' S
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR7 C; Q' W+ b3 H* S3 D7 d' W
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
) I# i9 v5 k9 j# ?4 s  V                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
6 K  S2 i( j, y. `3 X3 h; T$ @                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS. S( G- q: @0 Q$ R! t
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
( H. f! c) c! A; |; F0 f/ d: ?$ ]( U                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
) t! l# O- C, C+ Q- O* Q: q( Y                     TRPRAKK") b/ |+ ~. M2 H$ T0 L- u$ [
     promoter        complement(10164..10944)$ a- p% U: f+ {) ^" T. E" ~5 ]
                     /note="CaMV35S2; CaMV 35S promoter, duplicated"
/ D8 L$ W  y/ S& c% T- _9 s     promoter        11079..11133
& ]! F; f. P% L1 X+ o                     /note="PlacZ; lacZ promoter"/ j3 u; H: O: n% H0 [
     CDS             11161..>11359
! {% ^6 V2 _- C0 z7 y                     /codon_start=1/ q# H( W8 T( G3 `& |
                     /product="LacZ alpha fragment"/ }. R: n  b) J) f) H8 R; p
                     /protein_id="AAF65331.1"
$ L$ o! u' l9 h: Q! j: O                     /db_xref="GI:7638058"
* I, d, u8 d& N! b' f. {                     /translation="MTMITNSRGSVDLQPSLALAVVLQRRDWENPGVTQLNRLAAHPP4 A4 g+ S3 E) Y. y# b
                     FASWRNSEEARTDRPSQQLRSL"7 `$ c% m0 n: U% A
     misc_feature    11175..11210
9 m% S% i' g( t0 s$ l& y% X) X                     /note="pUC8 MCS; polylinker"- ]' e# V! D; p0 Y0 }7 ~; @% j
ORIGIN      
  {3 L9 y  g7 T% M, |) a! s        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct1 W1 p% ]) `/ E) X
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
0 Q* o3 a0 X' K  d' g" m      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt7 e1 W0 s& q0 y/ @) P% d9 b# P/ |9 c
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
7 d* x2 s+ S0 T, a      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
. A! w2 d$ x. U- D$ {7 D      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag* u+ K1 t4 l- \  D6 C$ r
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
2 R3 Q, h3 `. ^3 s, X/ [2 l3 p6 h' s      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg; r0 O% W4 k! F' B/ ^4 [
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg( v& b* m/ G+ b
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
8 E# ]2 W  X3 U5 ^' _9 X( {      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
- C2 z2 r9 f3 U8 C) T      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
  U% U) N* R8 W( @3 I" f# `      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg4 b$ r1 ?# V4 e- r8 p
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
( X% ]; N% y+ [$ v1 {8 X. ~% h      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
# o, h6 u4 t, {2 N! |! m8 G9 ~      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc8 n% E. C, a2 _3 |2 e% R! V
      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg/ q! B# k& u# c, `" }
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1 ?5 b" S6 A3 a     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
' k& w2 s' m& D* H     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
& h1 P$ C+ U6 _6 F8 c* M     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
( {, X" O( ?# ^( Y     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca/ @& V7 Z- P0 g* ]: O/ _) ~9 o
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact/ ^! C3 D, U9 o* v
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
  a, r- l7 a& f: H% f     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
7 u; ^2 \0 V/ [     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
. Q5 {; p- y/ t5 n     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
4 n! C* C+ T) w     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac! w- |9 \! t8 o4 ?2 Q) |# [  N
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata$ Q1 S  G7 S4 q7 j0 U. @
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
  l' O2 Z8 i7 X0 T) H1 _     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat. T" `( A& K( r
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
# Y5 G& R9 x4 Q; T7 h" O     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga2 B# G% c8 t& p0 z1 A
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc4 R* i4 A. u3 c! [0 @; D$ v% h
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg- l* o2 X( H& L' y' l
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
; i1 d3 f; D  v: {+ }1 K- x, u4 B, [; [     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga4 j) A' ~  U1 Q, [/ r5 T* M1 D. x, N
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata- n* e4 R4 y3 e3 k: R/ W7 g$ C
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa. @. t# u! e8 V3 h+ M3 j8 q
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt& y- V& ]" S  r; N" t7 J
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg$ \$ h2 M7 @9 x
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg- I# W/ q! r( \1 o3 A
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
5 z* w. L8 F% U( t! B/ R* z     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc6 R8 h' E* o7 D9 J  s, j; a
     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
) h* `: U$ N' A; h     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
3 x( b$ N7 E3 v! E     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
, M9 L4 l& l3 h5 }5 O     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg' X. [) @: f9 ^4 ^
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga+ e% D" ]3 ]2 o$ H4 U$ T" Z, x
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc7 }. C6 |& I: a
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
9 h- Z; T' F1 B' v+ Z3 t1 G     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
, A9 `& m9 K$ o% J! o+ C" e     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
6 X* @% R2 Y- C/ Y2 R! i( C3 I( z7 X     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc9 A" s! P% m0 {: _
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
3 H9 v6 \6 k) s) [" x     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
" z2 l* v& I  _0 x* t     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt( r  j* }) H; c7 m. r: }% w7 a
     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac/ ?- f6 Q2 _+ R
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc3 X6 N. b% S6 t3 l/ d; u
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
& j& Z7 o& u" s3 k     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
+ Y2 a3 b, H7 m4 ^4 d: K4 G& s; J" e     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
& K4 X  z" H9 r  q, ^- z     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
( ]4 I7 Z- G5 |9 ~( ?1 T     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
3 G& K; P/ e2 [* k% D& m/ _     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg3 \- h2 ], T' a
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
- M) }' @6 j& R) K( ]     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
2 s3 q  I. T3 X* l; r: ]2 x2 i     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
) e4 h- n# j! q" u& h( G     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
* l  z9 T( i+ J2 R     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
9 a3 \, Y, x# |9 q; L7 W' L/ ]6 D     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
, M2 k! t6 f3 z5 U; \# H* f     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
! ~9 l# p/ H: N- D: o     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag+ c* H. i& A- x9 F( C
     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
, [  \& \7 O# z; n0 {; `9 i     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
1 K& j4 ^; Y) g/ C4 T- i     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
0 q9 a0 q$ g' E2 F( F& ]     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
8 B& ~1 a! i' s6 w) V4 O     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
3 i/ r; g; i& k. E     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt; a+ E; R, s) K3 w
     4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
! e9 l  C$ k; o4 ~! Z     4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc9 Y& H2 k' H& u# [8 m8 _% w3 `6 r8 b
     4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca+ a' t1 y, j# G+ c0 G0 }; D) z9 r
     4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
; _5 ?/ ?2 A4 J7 s' Y8 {     4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc; n2 o* W8 s4 g: T2 p- ~* ]0 b1 Q% C& {
     5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg  b; E' o8 M. h( K8 f
     5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
, ^+ @! J' s- e     5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac3 }: [& R% Y; Z$ B8 r. p
     5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc9 I2 r+ t3 P- c, {; S5 g; ]- l: i6 T
     5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
1 `1 e0 j9 |& f     5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
! p4 _2 f2 F5 r% ^- m  L( K7 |) M     5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc4 M- g8 I) J+ e. e2 e2 x5 ?
     5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg* {1 G0 V7 r7 R. l/ q7 D( U
     5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
8 k/ ?' L' |6 i& ~% ]     5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa. l+ c2 i5 z2 i4 T0 n  E: `
     5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
: e9 n5 T6 J1 \9 [, {. b& }! V$ D) G' A     5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
/ \4 g) g- [4 K  J2 _, {     5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag* t* V2 Y) f' F* f
     5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg4 I5 E  V! v  @
     5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
# L6 R' s, `! e* p0 L     5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
2 w) l2 s1 B* S     6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
& d4 @5 I" E; d     6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
. g2 @4 F' `; o0 K( e     6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
6 ]' b% R6 K* T$ F4 m1 R: k$ i     6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg, T: \2 h" e% y; ^0 [# h
     6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg6 o5 {" G5 j5 @; ~8 r
     6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
0 f( X# X0 K3 K0 v3 R7 q     6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc9 B8 q, g/ t! z- D, P
     6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag1 |; j  c  b% Z7 X" m1 k
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga7 l' X1 o# ^9 f& V
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt6 s; I/ K6 o7 N% i
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
0 t4 l- Q2 y+ O0 N6 E+ H% g/ i     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa& Y/ j; r! o! S  v% i6 f
     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
9 |1 k2 c5 `  O) B. c" ^     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
7 x6 D: Y' |* ]6 @( [     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
! [9 T/ a2 \# i; E3 p3 p     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
& [$ u' |6 B& V4 u     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
- s& W3 E4 C5 h8 U$ N. H9 v2 X     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg+ t" G9 j, T9 f* x5 y2 g* r
     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca6 J% l5 V! g. Q) f% T
     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
  E9 G. b( D2 Q, C     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa$ Z8 W4 }2 G& {" E- D
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa9 ^/ ?2 _8 {. q
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac  }1 Y; g. @: P1 m: R6 D1 z- I1 @6 f
     7381 tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat- A3 ]# D; e+ `; `- U+ Q
     7441 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt# q% g) X8 ~" B9 p1 _
     7501 gcttagtgca tctaatcgct tgagttaacg ccggcgaagc ggcgtcggct tgaacgaatt! B& W; k2 @( E, D: r
     7561 tctagctaga ggatcgcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
. M8 Q# o! u  k+ Y     7621 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc4 {0 ?8 ?/ ?2 l2 k" l$ S* K) R4 u
     7681 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc& S" U2 G  k$ D# J8 n
     7741 cattgtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt' l% D6 D* x7 s' o+ J  ]4 c
     7801 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
# D" V/ F; N6 j- K2 u     7861 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg: A" {7 c* M" V( _
     7921 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac& P/ M4 K- Q! o4 c; t& I6 m
     7981 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg; A3 F5 H7 Z4 E, \: ~4 ]: ?0 Y' P
     8041 gaactccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
6 t; H6 e7 V4 {     8101 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt6 K4 D$ y) Z+ m( i8 b+ b
     8161 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga) A* |3 ~8 g. c0 {% x# W
     8221 tatatcaacg gtggtatatc cagtgatttt tttctccatg atgtttaact ttgttttagg7 K# l: B4 O. E' O* F
     8281 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
& x" i: @; }+ z3 H6 T     8341 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacatgtcat
4 a  J2 k( ?0 \; O     8401 aacaagaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
$ k) [! y; ]; J* ?     8461 tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa ccgaacgagg- S( H  B3 W4 R8 {: {& @6 r% b: ~
     8521 cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta
' ^: h4 J  Q9 i4 z& r) i5 a- w, j     8581 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
( S; J+ h7 k6 v0 g6 K9 ?     8641 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
% e6 p* }) f* V     8701 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
* q5 f8 A# @  s0 y2 O% F     8761 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
0 C' A6 M. Q3 T/ r, d. k2 G. o( W     8821 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta
0 W. ~6 p/ P3 u     8881 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga) `: n: K8 M* G
     8941 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag# |5 g8 ~4 ~+ h4 c" \9 H; P
     9001 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag) m5 c6 t0 F# {4 B0 Q6 m. l! r+ O
     9061 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg
, m8 Z8 T3 G4 R     9121 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca+ P$ O9 h; [- D
     9181 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
4 L' D2 V8 ]$ q. s: z- B9 {1 m! |* W     9241 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct  E0 T- u8 R2 Z# i9 y) b9 y; z
     9301 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
( M: R+ ?4 a  s! [) P5 Y: p2 E: `9 Y& p$ ~     9361 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg& E& f2 I2 N: d
     9421 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca# D/ E' B- ^0 N2 Y5 K
     9481 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
" c2 u0 }; ]1 z) L     9541 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc' u  @# ^3 s* @( a2 P- u
     9601 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg! f5 y, t$ y8 h' B
     9661 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
5 T% Z, d0 d' _7 O7 ^4 W! b     9721 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc9 D( n- J' J! L6 J( Y5 |' q+ _
     9781 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc& F# x0 p% @, a; ~& ]9 X
     9841 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac9 `  S/ g. s1 L9 g7 w: ^. d/ C
     9901 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
3 H7 k) Q, l2 Z5 \* ^     9961 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
% X0 U/ \6 L2 t7 C" r    10021 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc9 A; O% M, ]' o; ?. ~0 ^
    10081 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc. z* G/ }! Z, N* W
    10141 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
5 l" u9 D  r# H- @7 m! {. ~    10201 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg. x$ U3 P; d0 v2 Y( s8 x
    10261 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
' R) U( {6 j" @1 L4 `) q    10321 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg& |9 N+ k7 h- y- |9 P
    10381 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
" I: O* x8 M+ T6 U2 e# M" Z0 \    10441 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg2 {0 d8 k, t( g
    10501 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
, F. O  w) z2 N+ N    10561 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt9 ]4 {/ ?* q$ c" ?$ U7 R
    10621 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
3 N. s2 @: c' }9 u+ g/ n    10681 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc4 t% P1 Y. C' Q
    10741 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
* C7 C1 `0 v" q( h5 l    10801 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg0 H& y+ f; K# P" u. f) c$ M& O
    10861 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta+ b. v' I' w3 k6 Q: b/ R
    10921 gacgagagtg tcgtgctcca ccatgttggc caatacgcaa accgcctctc cccgcgcgtt! E" i! l( Z. g
    10981 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
5 i  w% e: P5 n    11041 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
7 L; I% _. b- }8 R8 ^& P% f+ i3 A$ |    11101 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
: U. j, |- w5 w! X6 j& C7 x8 T    11161 atgaccatga ttacgaattc ccggggatcc gtcgacctgc agccaagctt ggcactggcc3 Q1 d5 ~6 G) M) K$ R2 [
    11221 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca) M# G  Z' T# D) \& @
    11281 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc. n) c( x1 M. I: m- J' Z7 H
    11341 caacagttgc gcagcctgaa gcctagggag gagtccac
. g- i  O# R" i4 F" ]5 {) {- y" ?- _6 h$ `& J7 M
[ 本帖最后由 wcg129 于 07-8-17 13:23 编辑 ]