[质粒图谱] pCAMBIA-2201

图谱库ID：5 A; U9 ]' r4 H7 P9 W* c
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质粒名称：pCAMBIA-22019 v9 o, x  j3 K0 C$ Q1 S5 B/ h
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载体类型：Bacterial" G+ b& o3 g# K. x
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  V  E2 l" u! }9 `7 n! U3 }' B4 N报告基因： NPT- d) U' L# `8 ^! A% A

9 U4 x) K- E  D! C载体图1：6 l! X4 B8 @/ h: u8 w

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/ z3 |: L3 h+ h- C  Z8 a4 ^载体图2：" c* o) k5 }3 }* p, N

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序列如下：- L" x9 u6 K$ U; k# X; ^; e
LOCUS       AF234314               11785 bp    DNA     circular SYN 24-APR-2000
& l" {+ A/ n* BDEFINITION  Binary vector pCAMBIA-2201, complete sequence.
4 y+ c3 E  q+ {1 j& K( IACCESSION   AF234314
! H6 w7 n4 a5 H0 U. x0 Z3 Y- eVERSION     AF234314.1  GI:7638140
, ^* i& G$ M. D" M9 Z: XKEYWORDS    .
6 n; T5 f1 s" \5 K$ v: q  \SOURCE      Binary vector pCAMBIA-22010 F; D2 n# h8 b
  ORGANISM  Binary vector pCAMBIA-2201
. Y/ D% _/ d% m5 l" n) n2 m6 k! I            other sequences; artificial sequences; vectors.
6 \" ]- t+ n+ z1 J, V# zREFERENCE   1  (sites)4 B# b8 S8 G6 u. e! i+ z( T
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
6 e4 j) k0 `" u9 K: K  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors0 u" y7 d* L0 P  h/ r% P
            for plant transformation" i3 [! p8 m9 P) s* m: D
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
) f! I) P: {! d! D. D; t' z   PUBMED   7919218
+ m8 V7 f9 W4 Y: _7 ZREFERENCE   2  (bases 1 to 11785)
- a" ]  {# s4 C. C  R6 @# G" i9 h  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,# j7 V+ _& e; U' R
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,6 w( G; M( p7 Z5 M6 i0 @
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
# h3 F! x) D) v& r            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.5 i9 g) _' I( L# ~/ H2 c
  TITLE     A comprehensive set of modular vectors for advanced manipulations
. c# T. V, S. `. I& ?            and efficient transformation of plants
2 o# @$ i! a, W4 ?  JOURNAL   Unpublished
: W2 b' F7 d! `! m) d3 @9 ]  REMARK    Full description of constructs
& T2 T. J( m* N  ]% X! RREFERENCE   3  (bases 1 to 11785)! c3 O+ i! }, H* X1 n# s
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
* ?* `9 x' G# p            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,3 e7 n9 I+ r8 b6 V
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
8 ^# z6 M6 _+ W0 }% T0 y            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
: Y+ o% w" \/ e  TITLE     Direct Submission) X# _5 p) f4 J7 x" x9 ?
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
9 K0 Y- q6 ^7 S( ~            GPO Box 3200, Canberra, ACT 2601, Australia
) D; _- a8 B5 v! Q5 h& u- NFEATURES             Location/Qualifiers
$ v+ t, ?4 U1 B8 d1 H4 \4 m+ j     source          1..117853 t: K) K! A( K9 \
                     /organism="Binary vector pCAMBIA-2201"# M% A0 _% K9 V, K' J% l
                     /mol_type="other DNA"; q) c7 n; ~! V- H$ c
                     /db_xref="taxon:118406"
: e; `$ F6 T$ @% r/ q1 A4 S     CDS             join(2..16,207..2054)
- Z9 R7 ?3 q4 a5 U* h4 O: c' e                     /codon_start=14 u5 y9 W) e. G8 ~
                     /product="GUSA, hexaHis tagged"
/ }( ]* e1 A! j& z! m' g  x0 y  e                     /protein_id="AAF65397.1"7 ]4 S+ q1 H. p/ b& M
                     /db_xref="GI:7638144"7 ]" F  C7 ^2 [9 I" k1 W2 ^
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE) V% h4 C" H  M& [
                     SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY. O7 y% u6 v5 h7 x# y
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
8 H$ r' Y7 p- W" K  }1 V2 |                     NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
# l9 J7 k! t8 F0 g0 Z& x/ F, G1 b                     DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR. [0 z) {) h) D' m
                     VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY
& |# T9 g' r; u  b# ^- ^                     RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
% u# ~3 G. o: S+ f0 G  ?                     QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT; f; J1 x1 M! U$ m. L$ j( Z1 ]
                     CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
4 P" \! ]$ [1 _- p2 R) B                     IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ: Y/ k1 x; G3 q( C
                     GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"4 t& _) O" R, c1 Y, Z7 l8 D- [
     exon            2..16
2 s( `! e0 S! u7 ~+ j                     /note="synthetic first gus exon (modular)"& T' M' [6 S: \; }8 {
     intron          17..206
& F3 I5 a/ {1 g) ?& D- }6 v                     /note="Castorbean catalase intron; modified"
! ~4 N8 G+ [6 q2 C* ~     exon            207..2054& N+ B& J4 _' P! M( u, N- j
                     /note="second gusA exon, His6"0 R" y8 v7 O" z6 M
     misc_feature    2086..2338& t. k% }* l, u0 b4 G
                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
0 E* h) ^+ n2 H- Y" M     misc_feature    2376..2401! B# G+ I) p& @; B' R9 u
                     /note="right border T-DNA repeat"
% ^" o9 N5 S" Q- [( E( t: o( K+ S     misc_feature    complement(3442..4442)
4 F  o5 O4 ^0 l" k! \  F' f                     /note="STA region from pVS1 plasmid"
# J5 [) T0 Z- a# T3 [. M" b     rep_origin      complement(5035..6035)
0 Q8 W: s: g' l& P4 O5 p% m( e                     /note="pVS1-REP; replication origin from pVS1"
5 d5 F- Q4 ]6 _, h" Y     misc_feature    complement(6445..6705)& O, e* @/ }, N. U# h+ k
                     /note="bom site from pBR322"
% }& y  m" u+ k' h; t( _/ w" ]8 ?     rep_origin      complement(6845..7125)0 k7 Y) S  @: ~; n5 ?# ^
                     /note="pBR322 origin of replication"
8 C* E8 v( i& J! _8 ^* l- @     CDS             complement(7600..8259)0 C0 ?/ E' e' O
                     /note="cat (chloramphenicol resistance) gene from pBR325"( A2 {' J! x" }
                     /codon_start=1
) d+ S- w1 }( P7 ?% @9 k                     /product="chloramphenicol acetyl transferase"1 B$ L0 u7 E1 L: L' D3 Y* X
                     /protein_id="AAF65394.1"$ j  X$ z7 P7 W
                     /db_xref="GI:7638141"2 _2 I) V" Z- `2 z1 e2 q
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
. C' [' @5 s# J' B( d                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
* P3 {0 t: U9 J7 @: t* Q3 _0 _: G                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV! U8 f- [0 o9 D* t& }5 v$ N
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
4 Q" n* b& a( S  p8 S# Q                     A"8 u9 Y3 l" ]& H- i- h
     misc_feature    8787..8812, U" M1 u4 D( h5 N2 |" l
                     /note="left border repeat from C58 T-DNA"
' {7 w5 T, W" k2 X- v     misc_feature    8879..9082
/ Q6 X6 P  ?( k+ M" I8 `                     /note="CaMV 3'UTR (polyA signal)"
  q" n$ R8 J& b& J7 K$ A( s% d, Z     CDS             complement(9120..9917)7 L) G, {* c8 t7 r* _5 R4 I
                     /note="nptII (kanamycin resistance) gene"0 ]6 q8 V- N" B! ^
                     /codon_start=18 M4 c8 B# y' v& m4 Y: f% ^
                     /product="neomycin phosphotransferase"
, _0 T. X$ y5 ~4 b. J                     /protein_id="AAF65396.1"+ J9 w% u4 O' e; l# |( Y3 Y
                     /db_xref="GI:7638143"
: T2 p7 W; s* f                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG6 v! u1 y( @9 B# g
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD2 k/ d0 k3 u6 \
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD: ?; W, B6 q7 F  i1 A; s
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR; J9 _3 J( U8 y% a, o% s' d7 {
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"7 m$ I- \+ o* {% G
     promoter        complement(9948..10728)" @( a: z$ j0 g; `6 A( I! r
                     /note="CaMV35S2; CaMV 35S promoter, duplicated". Q0 b) i4 P  @8 N: o" C3 h
     promoter        10876..10930" X: M1 E# Y9 N7 ]$ ?
                     /note="PlacZ; lacZ promoter"
* Y2 C9 g% x' ~7 l, g' g     CDS             10958..11191
1 N* W% j: ^9 V' h% h                     /codon_start=1
; d2 w3 a6 {) H; f) H% b                     /product="LacZ alpha fragment"
/ d( a0 D) Q5 M5 N6 S9 J- x                     /protein_id="AAF65395.1"3 V$ [  D6 [: L3 D# h, f' p7 w
                     /db_xref="GI:7638142"
  y( z& _; ^( Z' W7 Q5 ^" j                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN6 _, x) x5 }9 K" B( o& W& k
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"- x- `, Q. g( J7 Z8 o  c
     misc_feature    11000..11050
) l/ N) r- B0 A0 @4 R4 d                     /note="pUC18 MCS; polylinker") H# l! P, N0 j
     promoter        11242..117796 E0 f! \# N3 e9 [( @0 W7 F
                     /note="CaMV35S; 35S promoter from CaMV"
- X# {3 p6 c  P" sORIGIN      
" @) x9 p0 H2 j4 K" y        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct2 {* M! s+ b" j
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga" c  D  y/ m9 ?% P/ S5 C
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
2 w$ l! D/ c8 A. k9 g      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa9 [% a! Q5 f  q( i8 b, E/ Y- b7 G
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg& ?) R& d, J$ d* j: c
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag3 a9 @+ B9 G7 Z# ~9 B6 N4 H
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt1 @" p8 U0 Q4 _, \% {
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
( H# ~/ u! K2 H' o: H9 O      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg& j3 M" y# G1 i% s5 ]2 Q
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
+ G/ Z0 w. ?6 X8 @/ M- l- m9 L      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
4 @6 K& ~7 i  v+ @5 E      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg3 X0 [" v/ E! s! H( V
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg1 I% g- K0 ~& X, Q
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
# p% J1 E( q' ~  {$ H  ?# `      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag. R' g. ^8 `' e; X' g7 S
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
  ?/ ?; P5 t5 Z" P- C      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg2 \6 O" v: ?. o" e6 r
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
$ ]. H; N1 b3 o5 `; s2 e, T  ]) W     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg9 x/ G) t. V# q( |% |: e
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct0 a9 ~; U4 u/ P
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga& B3 l* v! h: K9 i4 K
     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1 S/ _3 S# {# W9 P5 J. q- B7 v     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
# R- X  I2 l3 Z7 v$ M- ^8 `     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga) M& ]6 Y8 ?/ z7 G3 j* i
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
" T3 k- w2 ~7 n4 T     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
7 ^( ]! s- m8 q7 S     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
: c/ b" K6 d6 E% Y6 ?$ j; f     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac9 Z" M0 G. X% K# M5 {' I
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata! c. R4 a- `: r, S0 r( r9 Q
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat- y4 D% x9 e+ V# D! `
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
9 W" D& F: p" X' S, n% ]* J     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
* d. X- R& E4 Q; ^* a8 Z     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga8 d/ M% B$ K/ U: l3 Q- K
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc3 C; F1 m) O0 R9 a9 w" S5 P7 c
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
5 T7 Y% b. L$ B" l4 r9 A1 D     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
/ }; S. G: M! N# _     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
) e" g4 t" L6 Y' I+ @  H1 u# L     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata/ A6 Q  P8 ~( U+ X
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
( J; B; S, ^0 a  x7 J     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt. U1 S- W+ Y; h8 v! o
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg4 [* n: w, s. w
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg  y7 V/ |3 a6 A
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
! ~. x/ R- h% M9 e. k     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
3 k0 c, o; y7 K" F- r' s+ k4 @     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
% ]9 c0 _3 W4 Y  \     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt2 x6 `+ W; V/ @9 Y- z' C
     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
. G3 h7 @: T" ]( W! G' y     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg0 c% H/ R' b6 q, Y8 r
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga: C$ B1 T% Z! {' Z) U* G
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
1 t1 Z) ?0 I; J8 {. A     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt  p6 m0 d  c: J. E
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
7 N$ L# A- f" t     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct! J# Y2 R  r: D4 W+ [
     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc4 }. J2 q" U9 \: X: x# x
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg0 L9 u" A  a1 M; s
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
$ t* ?+ K9 Z' m% T+ N     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
0 v7 X! d9 c: H4 D# J* K     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac: ^2 F, D, ?! {# q  k4 h
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc* u" t. [* E! h/ ~( l7 r
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta( W* J& n) Z; c/ d+ m
     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa* g- F$ T! @* T; ]. F' H# Y# x
     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
: h& i( y1 A% @2 `+ A* v' e     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
. p! `" F4 N/ p# `3 x     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
/ ^: g. `8 P3 h# ~8 g* f     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg" y9 t  u" C& {- N2 y
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
; ?3 N9 \2 X; R2 x, \; i; q6 N     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
( y7 o3 b7 C0 j7 s1 A8 t     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
; C; d* [" c1 Y; S     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
. [3 j, {) g$ M% w     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
5 q  L) r9 g; g0 \: ?9 T+ F     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg3 ]& l1 h" s! \2 r1 P" {% _
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg" n, \# S% {* M) e
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
; }/ z6 Z9 h7 o* Q9 ]* |) U     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt# `" A: [0 b9 L$ ]$ i# V$ H- N* Y4 D$ f
     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
+ G5 f  b9 B  _% o; I     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
& ~- Q8 p2 H& z4 n6 S- ^6 g     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag7 k( N( j' ]3 B6 S: U. Q
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
$ r/ {3 F/ _3 x! r0 o! t4 W  p     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt7 i! k" E3 \7 e* w. k% Q6 v% d* z
     4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
" M; C' M  q5 G7 F! a0 e6 _     4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
1 j' K$ @4 N& Y' @     4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
$ p8 Z1 g$ N; z# D; v     4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
6 }6 K8 d" F! X- a2 t$ R; M+ P     4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
" A  ]4 g1 s) r+ F3 _% t     5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg  s1 O" P1 y- F2 @4 I2 w+ }# `
     5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
. x0 y( H/ c+ n1 S     5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
$ V7 N0 t0 F1 N9 C     5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
% C' u8 \: D% C) T4 M8 \( {4 [     5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
, T3 X  V# m& z- X     5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt8 s- e9 [: h" J* {
     5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc) D# f( ?1 f5 I6 Y/ }- l+ r
     5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
& ?4 L% p! B2 w0 H     5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc3 `% `- b6 K0 h3 |5 {
     5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
( w. D3 F* ^: p4 f) {' {( N9 @1 R     5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg  p- d' d& x5 _# y! t1 m* H7 B* x
     5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta3 E  y% f: Y& W' A
     5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
) [+ _" q2 t) F) W     5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
7 C1 Y  m! P8 B1 h7 d- h# I     5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
  a8 t& ?# e$ j7 l) u     5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa9 Q( V3 }; t. _8 j3 h
     6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg- l- d+ ^) Q$ y' F2 d
     6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt6 f3 t) G! K! p6 y, B) h
     6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
$ Y5 l: _, l1 @' U3 f& v     6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
+ D; |" i8 o9 N7 ^$ g; I6 v     6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg6 Y$ N) _" v# \, F
     6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
* \2 X+ N1 U0 q     6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
; j6 \% k9 P" ?8 m4 Q% v5 G+ w+ q     6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag5 p. J$ }5 `# z
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga: A3 y9 ^* d( s2 c
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
. z) x3 C0 D; x2 `$ A1 Q# N; [     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca: Z% M: ?8 _2 o; `
     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
( r1 h9 P  @; _  Q, W  [     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
" w1 S( Y" C9 P' s/ M. x. V' F     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc0 L& k$ Z7 u6 q8 \% o
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
; g1 u; ~6 Y- U. V     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
. q7 d" z# ?; d( V# r% a2 C7 [" u- r     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
- J  |2 e" X+ J) c* Q     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
. U; \  @( Y" q# y     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4 P$ _) h# x, P. Q     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc! }* V! O; C% h
     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
& I! Q: @/ S! o) h' m     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa$ [$ y) Z9 `; ]4 e, `8 m8 s' g
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
0 S) V, u9 N1 y. G( c0 Y( C' j6 S     7381 tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat- x- {' G% `+ L, d+ A, Y0 x
     7441 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt2 v2 }+ X& C, \3 \( f
     7501 gcttagtgca tctaatcgct tgagttaacg ccggcgaagc ggcgtcggct tgaacgaatt) v4 I9 J4 \& {) T$ t6 M
     7561 tctagctaga ggatcgcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc2 G, h& q, U- l# L1 s/ j& M
     7621 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc5 a4 L  H' w- A$ s1 {0 G0 H; J5 t
     7681 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
) W0 y. N& J+ Y, i     7741 cattgtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt, c; b3 n3 Y' k& I5 Q
     7801 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
6 C/ D6 I) U8 ]5 P     7861 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
5 D; t0 a* B7 G! ]     7921 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac, n% |% N/ @9 z2 d
     7981 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg* s4 A, Q# z7 a* [: k4 ]/ j9 T
     8041 gaactccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
9 d: D" o3 Q/ V4 e# t     8101 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt( b1 q8 @- H( O/ p0 z9 z
     8161 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga( Q; \7 c. h' a$ q  d
     8221 tatatcaacg gtggtatatc cagtgatttt tttctccatg atgtttaact ttgttttagg/ [  }1 S' K% X6 e# k- {
     8281 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
1 j/ h# ^. ^9 C4 P     8341 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacatgtcat
' P# p+ D% l" m+ t7 J( t2 k     8401 aacaagaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc1 {! M+ B0 H% g6 f' L$ ]: B
     8461 tggaccagtt gcgtgacggc agttacgcta cttgcattac agcttacgaa ccgaacgagg6 c4 l( }9 ?: M+ _" f) p: Y
     8521 cttatgtcca ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta! Q% c- B5 O, \1 I7 Z
     8581 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
$ G0 f7 k. G1 D6 K     8641 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
; y" O/ e4 ?( c# z+ A     8701 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
8 j3 K/ ~5 E3 f% u5 I' P! B     8761 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
, I( y  ^" i0 E" Y     8821 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta7 j" n- W4 b* ~* |
     8881 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga7 m% L- O" j. y2 b; }
     8941 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
& A6 z( M' f: |9 D0 J     9001 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
) j' n0 s% g( |# R9 Y  V" E6 G& ^: c. {     9061 aaactcgagc ttgtcgatcg actctagcta gaggatcgat ccgaacccca gagtcccgct! w4 Z, k8 ^: N, l* v
     9121 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata  s* v7 @( c  e& {5 D4 V/ @: h
     9181 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
5 P5 M" z) P7 l- `9 O, E* z! {     9241 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat+ k2 w: u: s  g$ i' k/ ]
     9301 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgtgtcacg! ]: _6 x6 b& c0 T8 K& |( ^
     9361 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
9 Y" {* p, O/ v; G9 T2 A     9421 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
5 G# C+ O+ ?3 r: Y: ]     9481 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
5 J' I7 b! N3 k9 e     9541 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga# L! S  X2 ?! l
     9601 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
2 l' S0 E5 w- H  t( ^& \% z     9661 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
0 y  N7 g! G9 _9 P# D     9721 gctgcctcgt cctggagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
1 f; m" O' u( q8 }7 j& {' y     9781 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
7 p; |6 A) Q" J8 q     9841 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca$ r; i( T0 V. l' A2 w( C$ h
     9901 tcttgttcaa tccccatggt cgatcgacag atctgcgaaa gctcgagaga gatagatttg
$ `1 @$ ]8 a' V4 U/ M     9961 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag3 W+ L6 a) g* O) E- |7 a  x* r
    10021 aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc1 U- P7 j; i" ]. f* \
    10081 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
" g% l/ {- r0 r- L    10141 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
* Q" i' q' G" s& q1 K5 X# p    10201 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
4 Q' s0 ?* k% ^' [( x9 Y  T6 C5 ~    10261 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
; H4 L$ C. x( |* H& s    10321 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac0 ]* |9 C% J4 ?* r1 F' N
    10381 gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg0 Y! [( D; z  [9 N- `+ E
    10441 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc2 _0 g% f& K+ m' i1 r+ r
    10501 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
% @/ v3 O1 l) d$ u! X( a, g    10561 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
% H8 u! b' S) K0 t6 t" m4 ?- Z    10621 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
' d8 P6 u* {- C/ y% Y    10681 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg! G: O+ I0 ], x3 k6 C
    10741 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
- x) Z/ A" f7 g' O! @7 M' V+ a    10801 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
2 ~" w6 N' S: e    10861 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga/ S  @1 X# I2 }$ v/ s- m  s
    10921 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgaattcgag
. |0 G- U/ t) I3 k. j. M/ q$ w* |    10981 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt! W9 I5 P9 o2 q
    11041 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
: h- ~* m( e. |- b* A    11101 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
6 R6 H6 i3 |/ d: v    11161 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
( w, ^# p9 t4 V, c    11221 ttcccgcctt cagtttagct tcatggagtc aaagattcaa atagaggacc taacagaact$ s5 T5 Y; s) [1 J
    11281 cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa$ T* m0 y% B: r1 k" T3 C% M: l
    11341 aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac& O0 a' _: Q$ L. H' R6 B  p/ I
    11401 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct& V: q, y* p1 ]; v0 a) o8 d: e
    11461 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg5 N2 w7 ?7 D$ j( e; e
    11521 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
  {( r! ^0 a* G- e    11581 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt) l- X& C6 `' ?) j3 I/ w
    11641 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
3 [- K' F# h' x) D  E8 e1 ?, j    11701 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt  Y! ?5 P2 G' |2 s' w* m
    11761 ggagagaaca cgggggactc ttgac
% V1 x( \6 c( ?: H/ f% F' t( u9 Z- K6 c/ p7 B/ C
[ 本帖最后由 wcg129 于 07-8-16 12:55 编辑 ]