pET-28c

载体编号：P0071 载体名称：pET-28c 载体抗性：Kan 载体大小：5367bp 载体宿主：E.coli 载体特点：The pET-28a-c(+) vectors carry an N-terminal His Tag /thrombin/T7 Tag configuration plus an optional C-terminal His Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3). 网站链接：http://www.merckbiosciences.com/docs/docs/PROT/TB074.pdf 此资源由“SSS”酷友共享，特此感谢！