菌株编号：B0020
菌株名称：
Tuner™(DE3)pLacI Competent Cells
菌株抗性: 氯霉素抗性
菌株特点：F– ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLacI (CamR)
Strains with the pLacI plasmid are appropriate hosts for pTriEx™ (1.1–4) and pETBlue™ vectors only.
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. In Tuner(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
网站链接：
http://www.merckbiosciences.com/docs/docs/PROT/TB009.pdf
此资源由“SSS”酷友共享，特此感谢！
