pCAMBIA5105质粒图谱分享
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图谱库ID:
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质粒名称:pCAMBIA5105
5 G3 R& P9 j; i' f" M% F0 x3 J5 N1 O, r7 i
载体类型:Bacterial
7 a- |' h7 `6 H- \1 q1 k1 f- B* T, d0 B5 D i# E" p
载体抗性:KAN hyg(潮霉素)
% `5 R, c6 J5 o) ~; O& p2 B
; v' o' J( @0 b) \8 B: d报告基因:HPT2 NPT
1 u6 V. z7 {0 q3 T7 G1 R) _! @: `2 |: f* P
promotor:CMV35S
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图谱:
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0 ?2 n f+ @& @/ N: i序列如下:
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LOCUS EF042581 49756 bp DNA circular SYN 29-OCT-2006
- [+ p# L* v6 A: ?. S6 GDEFINITION Cloning vector pCAMBIA5105, complete sequence.
6 m% @5 p. B/ }5 s2 x) TACCESSION EF042581
+ w9 z2 w8 o' o% ], E
VERSION EF042581.1 GI:116585208
& X( n4 u& a% h5 Q9 j
KEYWORDS .
% {1 o/ d* `" A) S4 }& ]. t
SOURCE Cloning vector pCAMBIA5105
( v/ ?9 x. y) ?# Q- n ORGANISM Cloning vector pCAMBIA5105
* a: @& s3 p* L) z% p, l
other sequences; artificial sequences; vectors.
( U: E) p$ h& }8 l( G' oREFERENCE 1 (bases 1 to 49756)
2 L2 C8 f/ k. z% f- X
AUTHORS Jefferson,R.A., Jefferson,O.A., Smith,L., Baillie,B.K., Raines,S.,
! b3 z' ?* g c2 ~) T: i Ulkir,B., Tassie,A. and Tian,L.
q2 n, r+ u' S% v; [! U S/ b$ t0 S; F TITLE Freedom to Cooperate: Transbacter as a Biological Open Source
! w" l- Z5 ?: w" S, D" ^. p
(BIOS) Tool for Gene Transfer
$ Q6 m: W, u s
JOURNAL (in) 8TH INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY
) T+ q+ L$ H$ P. W ABSTRACTS: 35;
( \4 u6 x6 b# e8 e9 v, w, G7 F
(2006)
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REFERENCE 2 (bases 1 to 49756)
7 p/ j4 m" h+ n0 H AUTHORS Jefferson,R.A., Jefferson,O.A., Smith,L., Baillie,B.K., Raines,S.,
4 n, P- E& j% e n
Ulkir,B., Tassie,A. and Tian,L.
: W, e$ Y( | r1 \2 W% i6 x TITLE Direct Submission
" k* n# ?3 ]1 x, e* e% z3 `$ ^/ S0 M
JOURNAL Submitted (04-OCT-2006) CAMBIA, 401B Clunies Ross St, Canberra, ACT
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2615, Australia
* k# `& H* a9 }% n. wFEATURES Location/Qualifiers
2 p# o& ^( o7 g8 X' t9 R
source 1..49756
0 w/ h6 M' ^: K- U
/organism="Cloning vector pCAMBIA5105"
, Z* }$ w0 C$ W4 W) c: o$ r /mol_type="other DNA"
3 y9 k- x$ H7 s /db_xref="taxon:407981"
- Z$ `* y) D0 P' W /note="GTbacK series"
2 r8 s2 \. O, d L
gene 262..699
* Z( O3 E# ^4 Q: B' k; F' w
/gene="virK"
. k+ j( X0 W6 y CDS 262..699
8 F! @# i8 d& |# |
/gene="virK"
9 X# f3 g: l/ b* u3 |
/codon_start=1
6 r, t* [* T. I /transl_table=11
5 u$ j) Y6 O! W$ z( m$ ~! m
/product="VirK"
) _- k6 M* a) J/ R0 x
/protein_id="ABK01290.1"
; Y' U; _- Y) |2 m /db_xref="GI:116585209"
H: z/ }/ W R- x- i
/translation="MKRISTILVGVFLAAPVYAADNIHTLGTLSEIELALTAGKPVNV
1 b* K2 E% [; j9 m
TVDLSLCAPGVADTPATKTQGGMRIDAYRITTDGTLAFADQHFTIDRDGKPITQFIRY
8 e. i. p" o$ r3 j, `7 D1 c QIRSNGDADFTMVTFNMPTYERKGTSLAYKCAIDHGLSFRTPQ"
0 ~/ Y) t. V: b
gene 2014..4503
+ Q9 ?7 n0 h. } /gene="virA"
* w+ z+ C8 h( N1 |4 l( U
CDS 2014..4503
, C$ K" U3 y2 d- Q e) [
/gene="virA"
3 O2 R$ B( N+ O# a, K
/note="sensory component of two-component signal
+ X/ b+ s- N8 T" b$ i: C _
transduction system"
" v, _ I1 f% Q- t. n
/codon_start=1
1 Y6 z* S- y7 @ /transl_table=11
4 W% B) a; Y6 m/ O$ B/ A" n* F /product="VirA"
' t8 O; x R. p* S: O0 k3 r9 ]; v
/protein_id="ABK01291.1"
# G( z( A, [2 [' k. K7 a0 r" a
/db_xref="GI:116585210"
( f5 t" O6 `7 f) v* z; x6 Z /translation="MNGRYSPTRQDFKTGAKPWSILALIVAAMIFAFMAVASWQDNGT
1 I$ B: e1 `/ X+ D/ U- t TQAILSQIRSINADSASLQRDVLRAHTGTVANYRPIISRLGALRKNLEDLKQLFRQSH
+ P9 j( { `5 L- I- ^ IVSESNAAQLLRRLEVSLNSADAAVAAFGAQNVRLQDSLASFTRALSNLPGKASSDQT
8 i; q8 M! g: L5 M- g2 ]' P LEKPTELASMMLQFLRQPSPAISFEISLELERLQKQRGLDEAPVRILSREGPIILSLL
7 U- p a3 U! W4 n3 j/ R/ h! S
PQVNDLVNMIQTSDTAEIAEMLQRECLEVYSLKNVEERSARIFLGSASVGLCLYIITL
" ?1 K" q' z3 R Y- g! N
VYRLRKKTDWLARRLDYEELIKEIGVCFEGEAATTSSAQAALGIIQRFFDADTCALAL
( S; n5 c$ W8 \0 D
VDHDRRWAVETFGAKHPKPVWDDRVLREIVSRTKANERATVFRIVSTQKIVHLPPEIP
7 r+ K2 l9 `5 e4 S: u9 ?2 a q" i8 V GLSILLAHKSTDKLIAVCSLGYQSYRPRPCQGEIQLLELATACLCHYIDVRRKQTECD
2 s: l5 o8 A+ D( V5 \ VLARRLEHAQRLEAVGTLAGGIAHEFNNILGSILGHAELAQNSVSRTSVTRRYIDYII
! N9 G, h* D4 @2 M; f6 g SSGDRAMLIIDQILTLSRKQERVIKPFSVSELVTEIAPLLRMALPPTIELSFRFDQMQ
- h3 t0 k* H4 p& N SVIEGSPLELQQVLINLCKNASQAMTANGQIDIFVGQAYLPAKKILAHGVMPPGDYVL
! @+ @- w K+ m( F1 Y ] LSVSDNGGGISEAVLPYIFEPFFTTRARNGGTGLGLASVHGHISAFAGYIDVSSTVGH
, l3 ^- E' o0 V7 Q4 }, k$ V1 u GTRFDIYLPPSSKEPVNPDSFFGRNKAPRGNGEIVALVEPDDLLREAYEDKIAALGYE
* w: i' E/ `4 {, o2 J PVGFRTFSEIRDWISKGNEADLVMVDQASLPEDQSPNSVDLVLKTASIIIGGNDLKMP
" v, m1 F+ l G- Z. Y8 f LSRENATRDLYLPKPISSRTMAHAILTKIKT"
( B8 ?0 v+ D; L gene 7828..8571
( I) R$ x0 r- y) t$ A /gene="virJ"
. B8 }$ b1 l A; ^
CDS 7828..8571
- p) W8 ^3 j! o3 w& y/ @ /gene="virJ"
- }: e y: ^ l; g! l' L8 H
/codon_start=1
- k. \: L& u( {; V& x) m- n
/transl_table=11
5 s/ D6 b. I3 y+ H9 X- p /product="VirJ"
# v. x9 [, \7 A5 b5 i$ k: L& l% x
/protein_id="ABK01292.1"
2 p" \% X( v4 U; _0 p
/db_xref="GI:116585211"
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/translation="MAIKLVLILVFTLFPAADAAYANDRANGFMWSNGGETGVRLPLR
0 ~$ e8 r) y( ]
VFNAKPAKNTVAIIYSGDAGWQNIDEAIGTYLQTEGIPVIGVSSLRYFWSERSPSETA
" T; j7 Q' K O5 t. _2 I) w0 k KDLGHIIDVYTKHFGVQNVLLVGYSFGADVMPASFNRLTLEQKNRVKQISLLALSHQV
2 q7 {, b. {2 ]0 Q. ^! I/ l
DYVVSFRGWLQLETEGKGGNPLDDLRSIDPAIVQCMYGREDRNNACPSLRQTGAEVIG
% _% p& S0 J w0 l, i( z$ f% }. [
FSGGHHFDNDFKKLSTRVVSGLVARLSHQ"
0 U3 W0 T. D$ j- y" f- q
gene 10048..10767
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/gene="virB1"
8 B0 |: e0 F) d. b' e+ {
CDS 10048..10767
' V9 E9 Z- e9 ^: c& r5 T- f. a) D /gene="virB1"
' T r( c, s" R( x" Z /note="T-DNA transfer"
, K% X8 K0 s0 ?3 \ /codon_start=1
! f1 q8 S6 K8 z4 Y
/transl_table=11
. S& g! A) u/ u5 H* _0 M; {
/product="VirB1"
8 d8 e6 |0 z5 m1 x1 [) J
/protein_id="ABK01293.1"
) J8 U$ {( ^* Z' N# C& Q; _7 ]5 }
/db_xref="GI:116585212"
& u# w$ ]1 f* E+ S7 T; ~4 R
/translation="MLKRSGSLSLALMVSFCSSSLATPLSSAEFDHVARKCAPSVATS
# Q" l, a! H b8 H3 b: a4 c6 e TLAAIAKVESRFDPLAIHDNTTGETLHWQDHSQATQVVRRRLDARHSLDVGLMQINSR
. L! R c& ^( X9 s5 l0 b) I NFSMLGLTPDGALQACTSLSAAANMLKSRYAGGETIDEKQFALRRAISAYNTGNFIGG
% l/ ?! z; o* j6 Z& M0 @
FANGYVRKVETAAQSLVPALIEPPKDDHEALKSEETWDVWGSYQRRSQEDGAGGLIAP
2 l9 b! J! ~2 d9 L: X" V
PPPHQDNGKSADDNQVLFDLY"
2 q* P) T* t8 n9 B7 ?& D
gene 10782..11147
! F* E6 Q9 l1 J6 W1 B0 D) i /gene="virB2"
4 ` P: a. d* {1 m1 _8 [+ f9 q CDS 10782..11147
1 g1 n# }; [# T$ c. H$ o5 T /gene="virB2"
r7 s+ T/ A5 \ /note="pilin protein precursor"
- b$ U/ F, V3 M) Y1 @: F /codon_start=1
4 W) y7 a1 {4 v7 v. s /transl_table=11
6 Y5 Q! b! n9 _$ n& }
/product="VirB2"
( O/ B" u# n5 F. p8 s
/protein_id="ABK01294.1"
. G( [3 n$ A6 c& L# b
/db_xref="GI:116585213"
0 O6 ]7 ]% R" ~6 n# C( `
/translation="MRCFERYRLHLNRLSLSNAMMRVISSCAPSLCGAIAWSISSSGP
' E3 m" X5 F( o1 e3 C AAAQSAGGGTDPATMVNNICTFILGPFGQSLAVLGIVAIGISWMFGRASLGLVAGVVG
7 L, D b1 f' t/ P) U$ M5 f
GIVIMFGASFLGQTLTGGS"
q4 W \3 m' b V/ _# x gene 11147..11473
" w! A; ~. Y# p: w% u /gene="virB3"
) U. q/ F+ L9 p2 f& j: ~
CDS 11147..11473
5 ?4 o% u% d) i2 c4 c' n. y: p /gene="virB3"
- u7 \ f7 O7 L7 ^6 e' S, c
/note="T-DNA transfer"
6 t1 \; c* b7 m /codon_start=1
7 s) D x8 X8 k! X h) ] /transl_table=11
3 @5 J( R, c7 c; e /product="VirB3"
* e; o1 _2 ~# G* s$ _$ s /protein_id="ABK01295.1"
5 D; P8 e' d: d3 f
/db_xref="GI:116585214"
0 x7 L& K$ j0 |/ l! S) r. `! H /translation="MADRLEESTLYLAATRPALFLGVPLTLAGLFMMFAGFVIVIVQN
) n; f6 L) a( e* w" E
PLYEVVLVPLWFAARLIVERDYNAASVVLLFLRTAGRSIDSAVWGGATVSPNPIRVPP
- }. e0 D; t/ X3 |* ^& y: {- J3 B
RGRGMV"
8 N- S6 T$ g. E3 ]4 w gene 11473..13842
5 s. d8 F' |" A# p
/gene="virB4"
0 O7 V' X |0 z3 p CDS 11473..13842
; n2 w6 ^5 S( ]& v" X! c3 h
/gene="virB4"
1 [, e0 d8 h6 ]+ o7 z
/note="integral inner membrane ATPase"
( a0 p( c6 ~% s- r# h3 W% [ /codon_start=1
, H9 s+ S9 f" O2 C7 z6 N0 { E /transl_table=11
$ B4 N2 [6 e8 E' Q0 \( Q
/product="VirB4"
) U- U6 t! `. I1 l _' S- }
/protein_id="ABK01296.1"
( s6 o/ h! ?5 a1 r0 Y
/db_xref="GI:116585215"
' u( e9 C( C' m* V; v8 x5 C; S$ i /translation="MLGASGTTERSGEIYLPYIGHLSDHIVLLEDGSIMTIARIDGVA
8 W5 j! d) D' S# G' k$ c FELEETEMRNARCRAFNTLLRNIADDHVSIYAHLVRHADVPSSAPRHFRSVFAASLNE
% a+ X7 ?5 A% n, C' E
AFEQRVLSGQLLRNEHFLTLIVYPQAALGKVKRRFTKLSGKRENDLTGQIRNMEDLWH
9 j) z. h" t; M VVAGSLKAYGLHRLGIREKQGVLFTEIGEALRLIMTGRFTPVPVVSGSLGASIYTDRV
0 |' K- F/ `* R: @3 y ICGKRGLEIRTPKDSYVGSIYSFREYPAKTRPGMLNALLSLDFPLVLTQSFSFLTRPQ
( w. L4 ], x- T1 t" A AHAKLSLKSSQMLSSGDKAVTQIGKLSEAEDALASNEFVMGSHHLSLCVYADDLNSLG
% l) E" q# l3 l' W( M" N7 q, e' V
DRGARARTRMADAGAVVVQEGIGMEAAYWSQLPGNFKWRTRPGAITSRNFAGFVSFEN
; S( T9 V1 W, W5 P1 W
FPEGASSGHWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMMFVLAML
. j& s9 e1 S- f0 {- x: ^
EQSMVDRAGTVVFFDKDRGGELLVRATGGTYLALHRGTPSGLAPLRGLENTAASHDFL
$ a+ D4 Y/ V' h/ h
REWIVALIESDGRGGISPEENRRLVRGIHRQLSFDPQMRSIAGLREFLLHGPAEGAGA
8 {/ z7 Z, P, I
RLQRWCRGHALGWAFDGEVDEVKLDPSITGFDMTHLLEYEEVCAPAAAYLLHRIGAMI
+ s( o0 r- z. P DGRRFVMSCDEFRAYLLNPKFSTVVDKFLLTVRKNNGMLILATQQPEHVLESPLGASL
& J i, I/ B9 A' H0 _4 H5 [# s( a! y
VAQCMTKIFYPSPTADRSAYVDGLKCTEKEFQAIREDMTVGSRKFLLKRESGSVICEF
& C/ D( s5 i8 E3 V DLRDMREYVAVLSGRANTVRFATRLREAQEGNSSGWLSEFMARHHEAED"
: b2 r7 l$ X+ b& B" n8 d p0 X+ }
gene 13857..14519
9 F& J( p- _4 n" V$ p /gene="virB5"
! y5 i8 [6 q9 S2 \8 D/ e CDS 13857..14519
/ A$ |4 y9 x, w. ~! D$ o
/gene="virB5"
j* l' C' G5 o9 {( @. d
/note="T-DNA transfer"
, o+ V) ]& b- N1 D% V /codon_start=1
" J& y3 l e1 `4 O
/transl_table=11
0 d2 l) F* X- v( O/ G2 U$ \' ] /product="VirB5"
1 s" D- I6 c5 Y- m; [ /protein_id="ABK01297.1"
* H' q( D7 T6 `- z. ] /db_xref="GI:116585216"
, B1 f* y; u G$ \, J& H9 u /translation="MKTTQLIATVLTCSFLYIQPARAQFVVSDPATEAETLATALATA
% W6 O& J5 L9 ` ENLTQTIAMVTMLTSAYGVTGLLTSLNQKNQYPSTKDLDNEMFSPRMPMSTTARAITS
; K3 t! f' |- r W' d' G
DTDRAVVGSDAEADLLRSQITGSANSAGIAADNLETMDKRLTANADTSAQLSRSRNIM
0 M" O/ G' p4 r5 d5 Q QATVTNGLLLKQIHDAMIQNVQATSLLTMTTAQAGLHEAEEAAAQRKEHQKTAVIFGA
; T4 O9 H3 v" H8 T" M: }8 A2 l LP"
, C" u% b6 o$ t' Z2 I& E
gene 14616..15503
! N- p# K! H5 G
/gene="virB6"
% t [. x$ n' `- c5 g9 Y Q
CDS 14616..15503
6 D0 d& I/ m% o) b1 C
/gene="virB6"
/ b" l. g& g" U* ]" w1 W. a" b4 N /note="T-DNA transfer"
9 w- F) `) p& D /codon_start=1
0 }4 t0 T5 x. ^, Y; E/ F+ \
/transl_table=11
$ b: P9 l5 {7 J! J. ]* H( i7 G$ x /product="VirB6"
9 K/ K5 @. B* Z8 S5 l /protein_id="ABK01298.1"
" H! V- l1 M8 I6 `* N6 l- g /db_xref="GI:116585217"
, V6 y( O" A A
/translation="MNFTIPAPFTAIHTIFDVAFTTGLDSMLETIQEAVSAPLIACVT
( C2 y6 Q6 S. X5 ]( }1 _ LWIIVQGILVIRGEVDTRSGITRVITVTIVVALIVGQANYQDYVVSIFEKTVPNFVQQ
6 ] B: `# p& G: a
FSVTGLPLQTVPAQLDTMFAVTQAVFQKIASEIGPMNDQDILAFQGAQWVLYGTLWSA
( s/ m T; m% U
FGVYDAVGILTKVLLAIGPLILVGYIFDRTRDIAAKWIGQLITYGLLLLLLNLVATIV
/ t6 n4 x' S" v9 D1 K; r" B/ x3 o
ILTEATALTLMLGVITFAGTTAAKIIGLYELDMFFLTGDALIVALPAIAGNIGGSYWS
. x) P1 U; `! j+ R
GATQSASSLYRRFAQVERG"
* {- `/ s, U3 b' P" u9 Y" g Y gene 15537..15704
! _" z* l; q o. [. o' @
/gene="virB7"
# ? T2 ]# z8 V1 s CDS 15537..15704
; a2 y* H+ ~; G% F6 j: b /gene="virB7"
( M% `* O: e: T$ D /note="T-DNA transfer"
" Z* q, I, @: C* n# S
/codon_start=1
8 D" {/ Z# e+ Q
/transl_table=11
& _( m$ a: X% m. s# U /product="VirB7"
7 y- ]4 y* ` J. j
/protein_id="ABK01299.1"
- E6 Z5 W. U n9 |9 F& x% X- W /db_xref="GI:116585218"
. f# X3 h( l( N: B3 F/ P/ p% M3 W /translation="MKYCLLCLVVALSGCQTNDTLASCKGPIFPLNVGRWQPTPSDLQ
* ^% b, A" B- X% Z LGNSGGRYDGA"
. @: {7 d1 S& g2 N1 p gene 15691..16404
1 M5 F! J7 j K# k1 a /gene="virB8"
% Z) Q9 z) ?: t' |8 X" f8 i: w
CDS 15691..16404
4 [4 E5 {- O+ ~' g! A
/gene="virB8"
- X8 t4 u1 m: D5 b; S1 m
/note="T-DNA transfer"
' U% g ~* \" q, B6 A1 I
/codon_start=1
7 l5 ?+ L5 y$ D3 d; |7 h
/transl_table=11
" k3 M( X3 h. d) p) C- R& l. [2 b
/product="VirB8"
" d6 m( q& M3 v9 T7 z' B; P) s /protein_id="ABK01300.1"
' r- D! H' X4 A* c/ H/ y
/db_xref="GI:116585219"
8 f- Y0 G4 P; |7 w0 \: B /translation="MTGPEYAMLVARESLAEHYKEVEAFQTARAKSARRLSKLIAAVA
( p7 a2 G. g5 [7 h AIAILGNVAQAFAIATMVPLSRLVPVYLWIRPDGTVDSEVSVSRLPATQEEAVVNASL
+ z: p* x- t6 Z3 {
WEYVRLRESYDADTAQYAYDLVSNFSAPTVRQDYQQFFNYPNPSSPQVILGKRGRVEV
( E$ G: d* {+ J9 r; v4 e1 Q, _
EHIASNDVTPSTQQIRYKRTLVVDGKMPVVSTWTATVRYEKVTSLPGRLRLTNPAGLV
& B/ x& I/ ^! k G" c; J* ~2 Z$ j
VTSYQTSEDTVSNVGHSEP"
6 O: j% i9 I" T0 U3 [7 x7 t gene 16401..17282
! Z/ f P0 I0 |' b! [0 V. h- R
/gene="virB9"
5 q/ Y5 X! y+ M7 j6 @" p CDS 16401..17282
: t4 k" _5 x# J
/gene="virB9"
% S* c& Q' t2 h* F, z7 ]1 l; d5 d- J
/note="T-DNA transfer"
: w! E$ E5 r. W$ s! G% [2 j /codon_start=1
7 M0 _1 U/ ~7 _: ^/ ]! ?6 P2 T /transl_table=11
r& g5 _% l1 X# h& X /product="VirB9"
/ B6 P, e# d/ V4 X) O" a
/protein_id="ABK01301.1"
% u' F- ^, X Z
/db_xref="GI:116585220"
1 v4 R9 R! B3 f- I, Z# K
/translation="MIRKALFILACLFAAATGAEAEDTPMAGKLDPRMRYLAYNPDQV
2 U4 J9 u5 p- f$ n. }& f
VRLSTAVGATLVVTFATNETVTAVAVSNSKDLAALPRGNYLFFKASQVLTPQPVIVLT
- b% N6 `) [9 W+ ?" w) [ ASDSGMRRYVFSISSKTLSHLDKEQPDLYYSVQFAYPADDAAARRREAQQKAVVDRLH
% z9 F1 H+ h+ g1 W AEAQYQRKAENLLDQPVTALGAADSNWHYVAQGDRSLLPLEVFDNGFTTVFHFPGNVR
" T* u3 a5 Z1 h" l( w
IPSIYTINPDGKEAVANYSVKGSDVEISSVSRGWRLRDGHTVLCIWNTAYDPVGQRPQ
% m( x K' r: u* P6 M2 ] TGTVRPDVKRVLKGAKG"
. ~' a- a; s! u" Y. F9 Q gene 17279..18412
8 w0 e0 _( m% `: L' s! ~
/gene="virB10"
2 q( S5 {/ ]- w/ l: X7 C: C) o
CDS 17279..18412
* z0 F$ W: K2 z2 j* B
/gene="virB10"
: i3 C/ c; G, F4 ~3 e1 W2 V" G4 ` }& u /note="T-DNA transfer"
) g: C( _: t# ]6 q$ M' J' b /codon_start=1
0 y" }4 X1 M; J! | /transl_table=11
* D& s6 J+ C+ i$ L8 c+ ]2 v* b /product="VirB10"
" a' f- ]) b# l( T1 v/ X, B
/protein_id="ABK01302.1"
. S( P$ ?3 _. H2 U /db_xref="GI:116585221"
$ h) i( [0 ^$ q: t# T, D /translation="MNNDSQQAAHEVDASGSLVSDKHRRRLSGSQKLIVGGVVLALSL
1 G2 O- g* x* ~& A. z/ W SLIWLGGRQKKVNENASPSTLIATNTKPFHPAPIEVPPDPPAVQEAVQPAAPLPPRGE
6 X4 G) u3 F; Y2 b
PERHEPRPEETPIFAYSSGDQGVSKRAIQGDTGRRQEGKRDDNSLPNGEVSGENDLSI
: w; M! B. @1 I& C
RMKPTELQPSSATLLPHPDFMVTQGTIIPCILQTAIDTNLAGYVKCVLPQDIRGTTNN
# A4 U/ H$ y, Q P) N IVLLDRGTTVVGEIQRGLQQGDGRVFVLWDRAETPDHAMISLTSPSADELGRSGLPGS
/ u- s% B6 C* d' r+ J
VDSHFWQRFSGAMLLSVVQGAFQAASTYAGSSGGGMSFNSFQNNGEQTTETALKATIN
+ i6 s( s+ u" u) C+ t IPPTLKKNQGDTVSIFVARDLDFFGVYQLRLTGGATRGRNRRS"
2 R" ~! x8 z( I; C4 U( q9 b4 x3 N
gene 18452..19483
7 J; m; Y7 l9 _0 T /gene="virB11"
$ M- z0 V+ s/ G1 \4 d- P+ K CDS 18452..19483
+ X* J; d O" i2 ~: a9 G3 l. o5 j" s6 k /gene="virB11"
- T3 d! D9 m. \
/note="T-DNA transfer"
7 I3 y; U. V* _4 V" Y2 [: j
/codon_start=1
- `& L2 j4 K8 @. d5 {
/transl_table=11
( V; ]- w8 m$ _( d
/product="VirB11"
" S# Q3 t ]4 Z. }3 I5 G l$ `
/protein_id="ABK01303.1"
2 z/ W0 s6 ^: ?. e- @0 L
/db_xref="GI:116585222"
s8 n9 a- ~3 P: R" x
/translation="MEVDPQLRFLLKPILEWLDDPKTEEIAINRPGEAFVRQAGIFTK
0 j( O$ i1 J. P8 z8 K4 R MPLPVSYDDLEDIAILAGALRKQDVGPRNPLCATELPGGERLQICLPPTVPSGTVSLT
/ F* }# s; W; j( d
IRRPSSRVSGLKEVSSRYDASRWNQWQTRRKRQNQDDEAILQHFDNGDLEAFLHACVV
1 f5 b0 |$ t+ k2 f; D% H/ A
SRLTMLLCGPTGSGKTTMSKTLISAIPPQERLITIEDTLELVIPHDNHVRLLYSKNGA
1 J, P7 r1 f) L4 {8 X, y2 A& u
GLGAVSAEHLLQASLRMRPDRILLGEMRDDAAWAYLSEVVSGHPGSISTIHGANPIQG
( U* r6 I$ E; P5 C5 i FKKLFSLVKSSAQGASLEDRTLIDMLSTAIDVIIPFRAYEDVYEVGEIWLAADARRRG
) P4 {" @2 V/ t7 h* _
ETIGDLLNQ"
3 r/ k0 H$ {/ C4 k7 L0 k& ?
gene 19615..20418
9 k/ S8 p7 _& T /gene="virG"
8 B8 Z: c% B* S- n* H CDS 19615..20418
$ t+ @' ^2 @% |' G /gene="virG"
& p+ x3 T8 e9 E6 y0 N /note="response regulator of virA/virG two component
2 t( B$ }& K% }/ e: K/ c transduction system"
) @ E" r. b7 t- v$ a* [' h /codon_start=1
- b# ^0 r' W- r
/transl_table=11
# Z0 s5 E' V9 D% a& q5 ] /product="VirG"
: a2 n0 {& @$ \# p* w9 l8 j6 P
/protein_id="ABK01304.1"
$ T0 r2 g D( ^. e
/db_xref="GI:116585223"
8 C* x' c% r; f' _' X3 S /translation="MIVHPSRENFSSAVNKGSDFRLKGEPLKHVLLIDDDVAMRHLII
; k/ A8 ~4 A1 }5 J: M4 }# T EYLTIHAFKVTAVADSTQFTRVLSSATVDVVVVDLNLGREDGLEIVRNLAAKSDIPII
- U4 m6 J* P" y8 t' E$ U* P/ `# j IISGDRLEETDKVVALELGASDFIAKPFSTREFLARIRVALRVRPNVVRSKDRRSFCF
# ~+ g: e! ^1 f; _+ m
TDWTLNLRQRRLMSEAGGEVKLTAGEFNLLLAFLEKPRDVLSREQLLIASRVRDEEVY
% B% m0 G. p+ H( V6 o) [8 v. }" M DRSIDVLILRLRRKLEADPSSPQLIKTARGAGYFFDADVQVSHGGTMAA"
; [. R5 F1 }/ i9 E gene complement(20529..21137)
0 W# M# d2 ^9 F" k: R /gene="virC2"
- g7 \5 V0 T1 a5 E2 e3 h
CDS complement(20529..21137)
5 O: V+ {* E7 }. Q0 h+ u r Q /gene="virC2"
% d: m$ W" x' b
/codon_start=1
! M8 Q( t$ j; H$ q% _7 X0 G /transl_table=11
" g- b6 n6 m4 @/ O* C /product="VirC2"
4 o7 O) u0 h0 K) R- g2 y
/protein_id="ABK01305.1"
7 Z6 W4 }; C6 c+ S; ^ /db_xref="GI:116585224"
% ?5 a8 y! [, s8 _% x
/translation="MAIRKPALSVGEARRLAGARPEIHHPNPTLVPQKLDLQHLPEKA
9 m+ ~0 z6 j* ~; x2 H
DEKDQQREPLVADHIYSPDRQLKLTVDALSPPPSPKKLQVFLSARPPAPQVSKTYDNL
F5 W' m1 b9 _) L& ?
VRQYSPSKSLQMILRRALDDFESMLADGSFRVAPKSYPIPSTTEKSVLVQTSRMFPVA
% Y1 \" b# v- a6 h) R LLEVARSHFDPLGLETARAFGHKLATAALASFFAGEKPSSNW"
: z* g) o) w0 k; W% d5 i: |" A7 D gene complement(21140..21835)
8 U0 I1 ^& h: ], u /gene="virC1"
) v, J" N! {0 t" _
CDS complement(21140..21835)
: H B. y5 S, E: J5 d4 a* `" S
/gene="virC1"
; K$ H. b" ~0 U, U% m# \ /codon_start=1
. x. O2 H2 I }8 g, I. T /transl_table=11
1 D. E3 f( C; u1 m' X7 f /product="VirC1"
6 d! E1 y$ l1 P( M% G7 z4 A: J /protein_id="ABK01306.1"
- U% A' @, C, T+ O /db_xref="GI:116585225"
! Q1 T+ U8 F' Z6 y% H
/translation="MQLLTFCSFKGGAGKTTALMGLCAALANDGKRVALFDADENRPL
% f* x7 ]) s9 v+ c0 n/ f TRWRENALQSSTWDPRCEVYSADEMPLLEAAYENAELEGFDYALADTRGGSSELNNTI
5 r% k" E) L. Y IASSNLLLIPTMLTPLDIDEALSTYRYVIELLLSENLAIPTAVLRQRVPVGRLTTSQR
* O/ U6 }/ j! I6 n2 ~2 O' f9 d RMSETLESLPVVPSPMHERDAFAAMKERGMLHLTLLNTGTDPTMRLIERNLRIAMEEV
9 G7 J: r/ U/ \
VVISKLISKILEA"
* B2 ~7 h* W- l( c$ {
gene 22104..22547
, n& d l; W" t( P4 Q /gene="virD1"
- P8 d+ d' i0 n2 K% t5 @ CDS 22104..22547
, R0 D- O+ _& S4 i( D' ]( n /gene="virD1"
8 Q6 C- Y! r8 H( u# u) c
/note="cleavage accessory protein"
% r$ ^! v4 n1 ]6 N( m1 Y$ X: n /codon_start=1
$ l' C1 W7 P% I /transl_table=11
" b( ]6 J! \+ ` V0 J$ T
/product="VirD1"
4 o9 Q1 O% Z" w3 y- I! s /protein_id="ABK01307.1"
0 v% _# \; [ j7 X f1 }
/db_xref="GI:116585226"
2 `1 b- Z( }9 C2 s% F0 z
/translation="MSKHTRATSSETTINQHRSLKVEGFKVVSARLRSAEYETFSYQA
* B, Y! k) S5 T RLLGLSDSMAIRVAVRRIGGFLEIDADTREKMEAILQSIGILSSNVSMLLSAYAEDPR
5 M+ i; q) C) B* y1 Y1 v
SDLEAVRDERIAFGEAFAALDGLLRSILSVSRRRIDGRSLLKGAL"
- Z8 W7 e+ D# ^( \) \
gene 22581..23855
- A, U s- B' G2 f7 ?
/gene="virD2"
& s2 o8 F1 Z) a. e3 h L
CDS 22581..23855
P$ D4 L, Y9 [! _2 ^1 k. l
/gene="virD2"
- F1 R+ ^$ j% y5 \0 m4 u /note="relaxase"
- F0 K8 Q& |0 C8 Y6 I3 R1 } /codon_start=1
' l- X6 |( j9 S7 Z
/transl_table=11
. v! a1 G0 d" _/ V" [: V /product="VirD2"
! g) N4 [5 e, s, R0 A# p
/protein_id="ABK01308.1"
( m0 O9 n0 m4 Z9 A, \
/db_xref="GI:116585227"
. h# f1 F0 G f5 b5 r+ T
/translation="MPDRAQVIIRIVPGGGTKTLQQIINQLEYLSRKGKLELQRSARH
% F' Y& Q5 m W& z0 Z6 _, |0 \
LDIPVPPDQIRELAQSWVTEAGIYDESQSDDDRQQDLTTHIIVSFPAGTDQTAAYEAS
' ~ f3 `; w( a8 l( c REWAAEMFGSGYGGGRYNYLTAYHVDRDHPHLHVVVNRRELLGQGWLKISRRHPQLNY
$ k4 P, H, E4 l$ E) K$ u# b
DGLRKKMAEISLRHGIVLDATSRAERGIAERPITYAEYRRLERMQAQKIQFEDTDFDE
# K, W% m0 q+ Q$ A) J$ l$ x TSPEEDRRDLSQSFDPFRSDASAGEPDRATRHDKQPLEPHARFQEPAGSSIKADARIR
3 c& n1 e1 P; y* v9 Q, Y: g; r, M
VPLESERGAQPSASKIPVTGHFGIETSYVAEASVPKQSGNSDTSRPVTDVAMHTVERQ
# P6 j9 a- P# Y9 G( f: L4 {# d
QRSKRRHDEEAGPSGANRKRLKAAQVDSEANVGEPDGRDDSNKAADPVSASIRTEQPE
+ w+ M) I# }: B2 H: m
ASPTCPRDRHDGELGERKRARGNRRDDGRGGT"
" k0 v/ P& G* j T3 |
gene 23875..24513
' s, C% X. V/ u3 q2 i! J2 S
/gene="virD3"
' ~: m& r8 F3 T) q- | CDS 23875..24513
1 s5 K) c6 V4 N& q. j /gene="virD3"
% T( Q8 P3 y3 A* }3 T+ U
/codon_start=1
& b: ?( p8 ?" D: W' t7 b
/transl_table=11
% n. x& W7 A' v- e9 |) O/ r
/product="VirD3"
$ o7 ?. c9 j9 A; Z) m1 g% | /protein_id="ABK01309.1"
% j; A$ G1 O* D! o- v# J
/db_xref="GI:116585228"
9 N% L1 Q" I7 w& |5 y! ? /translation="MANGQFTIRSARPASVGLTGERRGAASASSSALSNVQRDVRDRL
8 K* y) b$ K; y [4 z z
IPTSSPRLPNAAILRDSSGRASTGLRYMAATLHWSAIAPLSLINSNDLAPAAYDFETR
1 K H2 [' P" w4 A* }0 y! k NNARNVTAKVGRAVPVPKQGGLGKTLAPVPLSTRISRVNSDRRLPADAEDRPETRDPQ
" j% k- P- j/ ]+ A- ]" E KGRGSHGATPTLHEKIGTAFARRLRKHTYYIVCSCCQTRSALTMGAKISVKS"
& M+ M; u0 ~2 D+ L4 d) A. Z
gene 24510..26468
7 q D6 z. N p i9 p /gene="virD4"
% z$ w3 \' z) P: r: x. M CDS 24510..26468
9 h3 F- h/ m7 U5 l c# [# W( { /gene="virD4"
/ `/ I' W7 F- h+ u7 a3 O /note="coupling protein"
) F. F2 H& |! |1 @& f0 V /codon_start=1
- f' P7 r! G, b. X
/transl_table=11
: u% q* ]0 g6 t. P0 \ Z
/product="VirD4"
/ I( f+ X4 D* P. r- l$ b9 U, ]$ F
/protein_id="ABK01310.1"
5 i3 O! O1 w# l; f6 i' O& c /db_xref="GI:116585229"
* Q# W/ D$ ?+ w$ Q3 G4 {% B1 n% [6 N
/translation="MNSSKTSPQRMTLSIVCSLAAGFCAASCYVTFRRGFNGEAMMTF
1 p; `) K$ {. P$ R l& z9 I' O DVFAFWYETPLYLGYASTVFWRGLSVVIFTSLIVLSSQLIISLRNQKHHGTARWAEIG
1 Q. c- b9 ^ y. } EMRHAGYLQRYSRIKGPIFGKTCGPLWFGSYLTNGEQPHSLVVAPTRAGKGVGIVIPT
) j# K: ^9 w9 U4 h5 v LLTFKGSVIALDVKGELFELTSRARKASGDAVFKFSPLDPERKTHCYNPVLDIAALPP
$ S5 }: _& w. K, k: R+ [
ERQFTETRRLAANLITAKGKGAEGFIDGARDLFVAGILTCIERGTPTIGAVYDLFAQP
: G9 Y3 U/ R2 G$ J2 {2 n GEKYKLFAQLAEESLNKEAQRIFDNMAGNDTKILTSYTSVLGDGGLNLWADPLIKAAT
6 y9 e( b! n: F4 p( p8 E! d1 |1 l
SRSDFSVYDLRRKKTCIYLCVSPNDLEVLAPLMRLMFQQLVSILQRSLPGEDECHEVL
k( T" W* n0 Y& \* A: R FLLDEFKHLGKLEAIETAITTIAGYRGRFMFIIQSLSALSGTYDDAGKQNFLSNTGVQ
' ?. K9 Y5 w3 V8 Z% J VFMATADDETPTYISKAIGEYTFKARSTSYSQARMFDHNIQISDQGAALLRPEQVRLL
9 V; j- `. P( W% ~3 S DDQSEIVLIKGRPPLKLRKVQYYSDRTLKGLFERQMGSLPEPAPLMLSDYSNDQVQYH
- v0 m/ r5 n* i LAPIANFNEDAAPQNRTVAEDHGSVKVGADIPERVMGINGDEEQADAGEIPPESVVPP
( L/ x1 {* U o s O
ELTLALTAQQQLLDQIIALQQRSRSAPA"
0 n5 J5 Z% g2 P4 D
gene 26569..29079
% j) ~, s( E& q* _. h% b+ N
/gene="virD5"
8 C) }4 h0 O& ^
CDS 26569..29079
' b. x; P8 r. Q- k /gene="virD5"
7 ~( A/ u2 f1 {! \2 S/ y |
/codon_start=1
3 ?: N" y( u3 h V' k# s
/transl_table=11
2 w5 p% D; b; T' v; o0 m/ \/ I /product="VirD5"
2 {$ m, ]5 W9 W+ R/ J5 C+ T
/protein_id="ABK01311.1"
; Q2 l! r- [0 {8 t) c0 d# a
/db_xref="GI:116585230"
2 n/ e& I6 l/ P8 d) q8 ~5 s% \ /translation="MTGKSKVHIRGSADALPDVPGGSTTAPFLTEPSRDQVDASFEVQ
8 q% M. D( p' P' v TDYSQSTSVSFTYDGVGLGPAERAAYENWCEPGRPTWKDLIIKARVDPIDDVTWLRDL
! d7 ?3 j& |7 t- p9 c% O EEDTPSTFRYEGMPLGIGERQAYENWQEDAQPTWEDLVVSARLTELGRPHGITGEYTS
- I+ b! F4 L5 l& b LAGSKNTSSISLKRKRSNLIDDENSSGSFSYDGMKLGEAERSAYGDWAEAEPPTWKDL
# o( {/ u' I* s- r+ ^ VLRARVSSINDSAWLFDSQTSSSSFEYNGVPLGEPERQALRQWQGDAQPTWEDLVVNA
: B B( q1 Z. t) _
RMAELCHAGWIEGQKGCFEERGEALPASERGSQRPIGQRTDSSDSFVYDGTRLGAPER
+ v, m/ {& M Y8 f) n& e9 X4 f8 e TAYERWSKRERPTWEDLILDAHQARTESDAVTTQAIGQSSSPVFLYEGKSLGDRERKA
. Z+ z! Q3 P8 r, |- }6 g YEKWRQPAQPRWQNLVVNARLAEIDPSAWIADERDPLDDSDALGRPSYTSLTDRSDVP
( E }% V; F2 s' c% Q
LDDQSIYRRSDLVREQVPESSQRQFAACSESETRPVQWFTASGSDANNTENITASDPV
% V3 m0 I- e, I DRTGGVKRLGSKSDRTVTASIHDVNSSTRRLLLNEFGSEAPRPSPEKTVRLRSDNIGT
- f6 }+ B0 O* f2 k+ u' ?
YGSRKNERARLATETGAYESEHIFGFKAVHDTARATKEGRRLERPMPAYLEDKGLHRQ
& g3 S, n7 y( n) k: [6 b HIGTGRGRTKLVGRGWPDDTSYRSDQRATLSDPVARSEGATASNGYQLNQLGYAHQLA
" A; r+ K" L+ D/ `
SDGLQSESPDGVALPIQVATTSYNYTVSRDPVLVPPDKNEAPQLLHLGPRGQTEAVLA
C4 }! k' G+ H" t RETALTGKWPTLEREQQVYREFLALYDVKKDLEAKSVGVRRKKKEVISALDRTARLIS
% w6 Q; w' _4 b5 D i0 T: U
TSPSKARSKAETEKAIDELDDRRVYDPRDRAQDKAFKR"
6 G0 w2 S) k+ m
gene 29529..29726
: E) n( S) U' ?) g3 X0 y+ k /gene="virE1"
: K( E! `5 j" {9 u$ {
CDS 29529..29726
8 i3 l1 U0 p5 p; R: @. z# V /gene="virE1"
+ O- v% l+ K- G6 ]( ?
/note="chaperone for VirE2"
) Y# t4 n( p6 |% w /codon_start=1
1 p2 X, \( [! t- D1 s O0 U
/transl_table=11
# X- o. D* ^( {7 p5 V
/product="VirE1"
$ @- p* Y: }1 h z/ g+ u$ F
/protein_id="ABK01312.1"
/ u" y$ H4 N# ^: n3 c. S: V
/db_xref="GI:116585231"
. S( J M/ U# j6 L1 {% F$ C2 m5 s8 Z
/translation="MAIIKPHVNKNRTTSPIERPESLIEEMSGSHPPSGFTNLDLAMI
# L: r: {% Y X: S! W% i( B
ELEDFVHRCPLPEDNLAGQKE"
$ n9 C% K; ?4 J2 Z: _0 R8 z gene 29731..31380
. b0 k; }& t+ f: U; c. `5 q
/gene="virE2"
/ S9 @' y, [% W+ G. N- V: { CDS 29731..31380
7 H- U7 W( S- P& g$ t8 i
/gene="virE2"
8 a7 X8 l3 K6 Q t- L /note="single-stranded DNA binding protein"
6 C5 [7 s6 | h$ _1 W /codon_start=1
' H; n; k" {, t/ P2 |; ^' J
/transl_table=11
, g# ^ B) B0 l9 D* M" A& G u /product="VirE2"
" Q1 _+ y% L$ d/ k# M /protein_id="ABK01313.1"
0 r9 b& Y6 _4 E& a; A2 k; [ /db_xref="GI:116585232"
0 C# h* L% F, R7 D3 t /translation="MDPSSNENVYVGRGHNIENDDDTDPRRWKKANISSNTISDIQMT
" V2 i; D$ D2 V* X4 I( T' i0 O$ r- M% u NGEDVQSGSPTRTEVVSPRLDYGSVDSSSSLYSGSEHGNQAEIQKELSVLFSNMSLPG
) M" ~7 u# S; L
NDRRPDEYILVHQTGQDAFTGIAKGNLDQMPTKAEFNACCRLYRDGAGNYYPPPLAFD
E( u4 v* W! A& Q# g2 y KISVPEQLEEKWGMMEAKERNKLRFQYKLDVWNHAHADMGITGTEIFYQTDKNIKLDR
; ~- A3 m" ~2 g5 w* P NYKLRPEDRYVQTEKYGRREIQKRYQHELQAGSLLPDIMIKTPQNDIHFVYRFAGDNY
& [! S+ m; a5 h( }5 n ANKQFSEFEHTVKRRYGDETEIKLKSKSGIMHDSKYLESWERGSADIRFAEFVGENRA
& i; k, H% ~+ ] ?1 n9 t3 g: d7 Q HNRQFPTATVNMGQQPDGQGGLTRDRHVSVDFLMQSAPNSPWAQALKKGELWDRVQLL
. C2 \; J( U4 {
ARDGNRYLSPPRLEYSDPAHFTELMNRVGLPASMGRQSHAASIKFEKFDAQAAVIVLN
5 q6 U" o" |7 D% m GPELRDIHDLSPEKLQNLSTKDVIVADRNENGQRTGTYTSVAEYERLQLRLPPDAAGV
; b" k3 C k8 I& f LGEATDKYSRDFVRPEPASRPISDSRRIYESRPRSQSVNSF"
6 }" W- v& [- ^2 `0 ? gene 31447..33465
, L2 D( H2 p. i0 l; \& r! V4 A9 d
/gene="virE3"
o# [2 M" V' U9 H5 a9 b# f5 U
CDS 31447..33465
7 u0 F+ F! b3 u5 U- `& }9 J, Q /gene="virE3"
) a: x" e3 U- Y
/codon_start=1
& e/ P& E, a9 `& P% K0 c* K /transl_table=11
. ^9 F# |5 l2 z /product="VirE3"
1 V! ?4 S' Z, s9 t' F- j' r /protein_id="ABK01314.1"
5 j5 _4 T5 D% |. O /db_xref="GI:116585233"
( j& S" I( D1 `: z" F9 W8 v4 p; g
/translation="MVNTTKKSFAKSLTADMRRSAQRVVEQMRKALITEEEALKRQAR
8 }% H8 v$ U s8 ~1 [5 } LESPDRKRKYAADMAIVDKLDVGFRGEIGYKILGNNRLRVDNHKELTREHGRLRKTKT
$ U+ Q" ~0 q* K$ C: ^
VLKRNPVTQEVYLGLYERKSWLSVSSHLYAADGTLRMKHVKYKDGRFEEKWERDENGD
# l+ c! S4 ? m5 Q3 a9 D
LIRTRYANRGRLFQPVSEKMGAPYRSGPDDRLYRDLTRRNGFRRETFERDDHGNLERI
- P: f$ c6 B `' \+ Q2 Q
GSNHVGFSKISVKAPNRQTSQTKIQKLGGAFNKSFRSLLDKEGNEMGRDILSHRRLYN
& C0 U& J' q$ W; [! I; O KRSAVYDEATGQLKSAKHTFGKIYRSETEYLSAGLKKVSKKILGVTVYRKFAALSERE
; I ]- ^7 |9 S8 z Y2 J- Y; D" ? SEAERLRSFESGAHRQIWQERAAIPGSPLPETDDIHFAQQSHLAKANPDHVEADVTRV
8 b! b; ~ T+ a8 {3 k6 G) \0 v TDQHVDVAGQTSSSPQRNLEGWLDSQSRYKPANMLLSNPDLQANGPRPYEGLAHLTLR
; @4 u, h2 }) S5 ?( [; A) b2 g RDNESDGHKENDQRLRHFSQPEPLVLPHPGSPEITKVFGSRGEPAHPSGNLHTAVGET
! U# p/ k' z! t! Z$ n. [
ACEGPVMSSSSDNHQPAPGQQELLSFLHNAPAPVSVAIHDDQERLAGEAPGGSFRGSS
" r0 c- V4 W7 G5 r, b% L1 Y- f
GRTSSMSESIFDEDVQGHLVRDYSINTTNGFIDPQSLFGEPDLSRGPKSGPEIPSEDY
4 I* f6 j) n( ~) D' L HLSASEQENLLNQLLSVPLPVPSPKPECARSMIFEGSRSRERSTSRGF"
# r2 `) N: }+ W7 N4 }4 k8 c primer_bind 35286..35301
7 R) \% q. A/ n, A) ^: r( v n( k /note="M13FW"
2 K! Q+ P9 r) s9 _0 l+ s
misc_feature 35442..35879
+ W' P+ R* ]0 B
/note="f1 origin"
, M8 |0 C6 S9 }
gene 36190..36984
; R$ S% ~" Y, r1 R" g( x( r /gene="npt"
3 n6 x _' y6 I CDS 36190..36984
" L/ }9 u0 @0 p c+ | /gene="npt"
, V) _; [5 ^- c1 h: r1 z# d2 G
/note="phosphotransferase; kanamycin resistance"
( k( J. V4 _) B: p" v /codon_start=1
/ K' d5 a# D; G S* v( R
/transl_table=11
0 n2 O( e3 h$ V$ Z3 H1 m /product="neomyocin phosphotransferase"
2 I1 \) J- U9 P /protein_id="ABK01318.1"
: d& {4 {. j: N! k" ?2 z
/db_xref="GI:116585237"
* i& k2 h* l* S/ [9 Q5 [, ` /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
q3 \. |! z) B* q5 U0 o5 r# x( f6 j
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
- A4 _. {7 d; Z
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
- U0 U( K/ } n" J, R! z- i EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
. h8 ]3 K. n& S. \ QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
5 V" |1 O9 }, l
gene 37935..38726
9 e. I$ R8 w5 W2 f /gene="aadA1"
3 V4 Z @9 g% d' t% v: r CDS 37935..38726
+ }8 D& X! d; t /gene="aadA1"
% b$ v7 I8 ~+ C M/ F" F: H' @ /note="adenyltransferase; spectinomycin and streptomycin
) s) Q& I+ D1 E6 [ resistance"
5 y8 n$ J6 v' \
/codon_start=1
7 d/ i% q, V5 q- ?+ W+ V1 C4 E+ B /transl_table=11
8 a0 |$ `+ n2 p
/product="streptomycin 3''-adenyltransferase"
/ Y9 p( g1 W) Q4 W( ]
/protein_id="ABK01315.1"
; E& J5 x/ X" `9 x K, h
/db_xref="GI:116585234"
- @1 q4 l- }7 W7 f$ G# B1 y3 o. O
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
( _, f, k8 R" o! e n! ~ HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
7 h: w% D n9 b AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
- b0 l5 g. [2 X! D& p
DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
0 z' m* h" M$ G3 `+ L% T& m$ @7 H
QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
! W! i, V/ H) W8 O- ]2 m' p rep_origin complement(39174..39454)
: u, d5 p% y) K6 x' }6 W /note="pBR322 ori"
2 w8 e: ]: L* ]( e4 \: M misc_feature complement(39594..39854)
, ^) e- h' P1 Q8 ^8 I) b
/note="pBR322 bom site"
+ E! s4 ^4 z+ F2 a) D0 Q" I) S
rep_origin complement(40264..41264)
\0 V9 ^7 d- ~: _ w
/note="pVS1-REP"
& D4 _+ E! V, B/ I9 X* R% k
misc_feature complement(41857..42857)
3 Y* k6 N" ^3 G6 X5 V! q2 ^* [
/note="pVS1 Sta; STA reagion from pVS1 plasmid"
1 m0 Z; @" L" z1 q9 ^- j' s- ~' M
misc_feature complement(43918..43942)
+ e( H7 Y3 s- [% {6 h /note="RB; right T-DNA border 25bp imperfect reapeat from
* x0 y$ G# _5 c8 \5 B5 A0 j4 f8 j
pTiT37"
; X2 l4 r A& H! R6 q& y
polyA_signal complement(43961..44213)
3 { `: T! v8 {
/note="polyA signal from nopaline synthetase"
& ^ n# P* n( V5 l gene complement(44235..46296)
$ p9 [9 E* M/ m, k- |" E
/gene="GUSPlus"
. o( Y! @. u6 E5 m( p
CDS complement(join(44242..46089,46280..46294))
6 C% S- Q# O% }! h- p /gene="GUSPlus"
) j, V4 x9 G9 G Z2 V Z# |5 Z# Q; H
/note="beta-glucuronidase; from Staphylococcus spp"
- R# v$ ?. ~" l. p8 q3 O- t /codon_start=1
& a" L, p7 d1 r1 y /transl_table=11
' S i# f1 X# G; j* Y! M /product="GUSPlus"
" }6 g6 T z; P6 ~
/protein_id="ABK01316.1"
3 ?+ Q: |. z( i: y/ `' \$ j9 A /db_xref="GI:116585235"
& ~ e$ d/ H2 g5 i! b /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYE
5 w# b4 Q. h9 r7 u* f9 [ SKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHK
& w2 ^7 H% B( K$ g6 H9 {
AIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHE
2 @' \! j2 o z3 T' s4 z7 t2 ]
EGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQ
3 |2 M# u) m1 c$ r GKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTID
/ u$ \: \) n: ^( `: r VYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWI
$ K M6 A9 d8 `, E GANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEK
5 b$ @2 c8 m! E% U- J IRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQK
" _: V! C8 {' e RPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRC
# g3 V$ x) k7 m; |9 v- y0 D
PGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFAD
( m" t% A1 G. R6 `
FATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"
% k g k5 v# C/ C% r8 U9 D- P intron complement(46090..46279)
- _8 Z* i- a* s4 s
/gene="GUSPlus"
0 ^5 I& W7 D3 z( @& p) ]4 W /note="catalase intron"
& l. q' \& C3 i- J s promoter complement(46302..46839)
2 D) ?. h$ f) ]# B6 w
/note="35S promoter; Cauliflower Mosaic Virus 35S
! B: a U2 [' Q* M$ ?$ `8 C
promoter, single enhancer version"
" z; @2 z5 L: q: L$ S
promoter 47332..48139
8 Q G* _/ d5 }+ o: b; p
/note="35S promoter; Cauliflower Mosaic Virus 35S
; ` |6 h9 \( W/ ?& L promoter, double enhancer version"
, @1 o) @" _0 @/ u
gene 48175..49200
0 [' p5 K0 a5 C! _$ B /gene="hptII"
7 d" H) `2 }; \4 `$ z) u: A CDS 48175..49200
3 c. O, G- E* C( a* Z/ F
/gene="hptII"
6 ?; L/ v' I9 {7 i /note="phosphotransferase; hygromycin resistance"
7 E" ^( E8 r" N% F" W' d
/codon_start=1
. l* A) F2 w/ A: w( Y5 I/ n
/transl_table=11
; @" h7 I9 k3 i* L! U /product="hygromycin phosphotransferase"
* t I" K; B5 U5 P /protein_id="ABK01317.1"
- v) n" i( a7 z, y- d) V/ }) T /db_xref="GI:116585236"
* k8 {4 d# j# F- d
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
( ]- H9 _( B- q1 @5 u GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
, f3 j% M6 B1 H0 [6 w; z
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
: [, L( P2 |' E. h+ l" e HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
0 p3 F" \) q6 k7 y! U/ A5 ?, w EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
7 I# `( p) ?5 ] SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
8 V4 {7 c" R* H R0 v! A TRPRAKK"
6 j" E& `/ }6 t1 A0 y; ~
polyA_signal complement(49216..49424)
# A j3 n) J, ]; w /note="CaMV35S 3' UTR polyA signal"
9 z; ^% ^ ~( q% x0 z
misc_feature complement(49492..49516)
( u% i. d% u- e
/note="LB; left T-DNA border 25bp imperfect reapeat from
2 |3 j' U' V, o pTiT37"
1 O7 c/ T& [9 s3 gORIGIN
3 i: @8 `2 u3 I( C) Z! \ 1 taatctacct gtgacactaa gatgatcgaa gccactttcg ctcaggagtt atcacgttaa
/ A, K5 l3 C4 M T+ u: z4 {
61 ggcgcaactc ataaaaatgc gtcaccgatt acttgatgcg cacgcttact atatctcaaa
8 [" K( x2 t5 O, U% P+ V, ^ 121 tcaaatgtcg aacctcggtt gaagcccatt gtctacgtcg cgtatatctc ccgtattttg
' v* o" e& J2 I @5 |" S
181 cttttgctgc cgaccgatac cgctgcggtt caatagttgc ggaatgtggt gctttcggat
- U8 O, O) v, Q1 `1 g! U5 d
241 tcactcaaaa aggagtcgct tatgaaacgt atcagtacaa tccttgtcgg cgtatttttg
7 g, O6 y# }7 ?( t. H! F
301 gctgcgccgg tttatgcagc cgacaatatt catacattag ggacgttgtc tgaaattgaa
9 M9 \- V/ A& u( f! W; q- p
361 ctagcgctca ctgccggaaa gccggtgaac gtcacggttg atttaagttt gtgtgctccc
$ P+ k. w {. ?) I( w2 A# D6 T
421 ggggtggccg atactccggc gacgaaaacg caaggaggca tgcgcataga tgcgtatcga
4 M/ w+ }1 K$ |2 x# P
481 ataacaactg acggcaccct tgcgtttgca gatcagcact tcaccattga ccgtgacggt
- O: ?+ H7 j( i3 A7 l+ V
541 aaacccataa ctcaatttat tcgttatcag attcggtcaa acggtgacgc cgatttcacg
2 _. t- ^" ]) p+ L7 S
601 atggttacat tcaacatgcc aacatatgag cgaaaaggta ccagcttggc ctacaagtgc
. x1 p5 K$ s- `& }% h0 r# _* f Z. i: N 661 gcgatcgacc acgggttgag ttttcgtact ccacagtgat ttttgcgtcc aatctgatcc
6 {: `. I* N% Q9 J. F 721 gatgcaagcc caaggtgacg cttgcaatta cccacatttg agcgtcgctg ccatttcggt
7 C. b' H$ }3 r+ A8 A3 ~" p5 b 781 tctgaggcga atatctgtca gccggctgag tccacgccag acgaccgtat ttcccggagg
" W1 Z' H, S) ~; A, r 841 aggatctgaa gttctagcaa ggttgccacc caaacgagcg agtttggtga gatagaagtt
( o# o5 H& J+ n I4 a+ ^7 n
901 tagcgattcg caacgaagca cgaccgcaga accacttact tcgaaggctt cgtatatatc
+ V' k! S6 D2 _! K1 f
961 gttcctgcag tgatctggct gcgatgaatg tcgattcgtc ctggtggtag catcagggat
+ r' a# N6 e! A! n* D
1021 agtcctacga cgaagcatag gatttaagtc atcacgtctg ttaaatgccg ccggcgctgg
$ D6 v) e R. Q6 H6 }* K 1081 ccacgcgaag acaaagagcg cctcgtcgct gcctgctttg agccgggcgc agttatctct
# v7 S+ D ?: B: u
1141 gagattgcct gcgcggacgg tatcaatgtc agtcagtcgg aagcatagcg atgtaataat
) {. q! X/ B. z8 R
1201 caagttgggt cgggaccgac gtgtccgcgt ggatagcaac atcgacacgg aagcacttgg
( N, m9 d' u% S 1261 ccgtattctc gattgcgtgc tggggctgcg atgatcatgg tccctgctgg tgtgaaggtc
% l- U; e! G# y5 R% j9 z% B2 _
1321 tggcaggcaa cgggctccac cgacatgcgc aaaggcttcc ccggtctgtc gttgatcttg
& ?2 m4 _% e' G 1381 cagaaagcac tgatgcctga cccgaggtgt gggcactgtt cgtattatac ggtcgtggcg
3 K. O8 L% q* ?1 l. N+ e: V3 {
1441 gccaacgacc gacatactgc atacggatat cgtttcgcga cgatctccta cagccgtcaa
! l( {) m! p$ c% q4 g! N' H
1501 agctttcgag cgcaagcgcc catcacgcaa actcttcccg gttcatctgt cgcgtgagcg
+ z' d+ U4 L [/ r
1561 tgtcgttatc gccaccccta cgaactgtgc ttgctgcgaa tccgccaaac tgtcatagct
. ~! M2 N0 o3 p1 p$ r
1621 tggcgaggat gttaccgaga cccttggaag tcatccgaga tttggcggcg gcaacctctg
* ]7 c: @# L) ~* @( }% f7 w7 H0 a
1681 caaaatgcgt tatgagcctg gtttttcagc cacagacaag tatcgcgttc gtgaaaaagc
0 s: w, p! ~# L5 Q: l& m8 [ 1741 gcacgcgcga tagttcttta gccgcacctc tgacgcccca tttacggccg gatctttgcc
* t' @1 R7 A( A# y2 } 1801 tgaccttgca aagtcgactc catcgcctta cggcacgaat aacaacgtcg cgtttcatcg
+ a2 q. ?: S6 h2 ?2 _ 1861 acgtcccatc agccgcttgt gtgttcaatt cttctctgtt tcacttgaaa caaattgaat
& \) X; _+ E/ w/ ~4 w$ \! k
1921 agatattccc gctttcaaag ccatttacaa atcctctcgt gcagcctaac gccattggcg
. {/ s7 l1 P+ K% P% @/ z- x& {1 D
1981 tggaacaagc gttggcacga ggaagtaagt gcgatgaacg gaaggtatt