酶切位点分析（DNAman）
# p- A% ]) D; o7 r# Y1 t/ u0 n+ x `1 zRestriction analysis on AF234299
0 c4 Z; `7 F& A" q) \3 r: XMethylation: dam-No dcm-No" a4 F0 y, H7 s* z$ z# v# y$ [
; z% E' Y' K& o; ^% B
Screened with 117 enzymes, 372 sites found( T8 Z+ G9 ]' }$ \/ h# V
AatII GACGT/C 1: 10464% T# L. ?) V' F. f
Acc65I G/GTACC 1: 11560- l0 o4 F7 }& d4 H! ]: T; n7 P& A$ Y
AccIII T/CCGGA 4: 9589, 9725, 10262, 120266 Y K/ n) T# D% `
AclI AA/CGTT 1: 4906+ Q5 M7 U4 H" b2 Q
AflII C/TTAAG 2: 2198, 2624
3 a) E o: B( d/ l* P$ {AgeI A/CCGGT 2: 6475, 10145+ n% F+ _2 ?) d8 d
AhaIII TTT/AAA 6: 2931, 6541, 8198, 8227, 8594, 8754) a; g& r7 Y2 B" }% c
Alw44I G/TGCAC 6: 950, 1275, 7008, 7506, 9901, 10203
4 f* `- e3 B8 b. S' M4 m9 tAlwNI CAGNNN/CTG 5: 859, 2504, 4721, 4789, 7608
5 N% q$ n! ]# O- G# m$ I: nApaBI GCANNNNN/TGC 5: 5437, 7017, 9036, 9910, 10186$ _" \& o4 U' Q! D0 @9 Q4 J
ApaLI G/TGCAC 6: 950, 1275, 7008, 7506, 9901, 102031 c) [$ ~9 ~% X& B
Asp718I G/GTACC 1: 11560
3 A5 b* P7 d: D3 DAsuII TT/CGAA 2: 1110, 2447
3 q7 S; {! G( h$ f, {5 ]7 sBalI TGG/CCA 7: 638, 1996, 3364, 4912, 5179, 5646, 6592
- Q6 [4 p" o( jBamHI G/GATCC 1: 115690 |2 U6 m. i" Z' Y: h& J6 }! F
BbeI GGCGC/C 1: 6770
3 Y, s% s3 d# N0 nBbvII GAAGACNN/ 12: 2346, 10683, 11010, 11991, 12216, 3062, 8230, 10686, 10911,
3 v* @: r0 h9 J: q2 O$ m! \ 11013, 11238, 12219
9 F9 I8 Y( ]4 z5 B% A, w4 P3 h4 jBclI T/GATCA 3: 106, 2362, 8909& f( ]9 J# Q2 J$ D1 o
BglI GCCNNNN/NGGC 10: 3236, 3622, 3856, 4044, 4056, 4065, 4918, 5331, 5643, 11754 ?' l* v9 l( r9 Z
BglII A/GATCT 1: 7
, ~9 ~' Y* t; O1 T2 g, m, XBpu1102I GC/TNAGC 1: 83029 Z9 l+ @" x9 A0 m
Bsc91I GAAGACNN/ 12: 2346, 10683, 11010, 11991, 12216, 3062, 8230, 10686, 10911,
4 {( f) {6 _4 v$ { 11013, 11238, 12219. z1 Y" H2 t. R+ g
BsiI C/TCGTG 6: 7365, 10715, 11042, 9904, 10383, 121878 x% t3 Z0 P% ~$ V# i! s
BsmI GAATGCN/ 6: 5153, 11765, 74, 5830, 7916, 8810
5 l7 @/ Y8 L- n' @6 RBsp1407I T/GTACA 2: 1137, 15632 ]1 b Z" d' M: F$ u& ]
BspHI T/CATGA 2: 901, 8452 G5 B" S/ o7 H8 c
BspMI ACCTGCNNNN/ 6: 1349, 5835, 6229, 11594, 619, 10170
6 c2 S7 s8 H: XBspMII T/CCGGA 4: 9589, 9725, 10262, 12026$ q: U/ V* `0 h( o% F
BssHII G/CGCGC 2: 2795, 2819
9 v0 l/ D3 c7 P; FBstD102I GAG/CGG 6: 3605, 7125, 11504, 6691, 9738, 10096( G% z; Y6 c' E4 P/ C5 Y: [
BstEII G/GTNACC 1: 25731 i5 B: N) ~' n) Z- ]
BstXI CCANNNNN/NTGG 1: 113056 ? q% j ~5 ?$ h
Bsu36I CC/TNAGG 1: 20910 `& ~: X, _( R9 o+ d2 h
ClaI AT/CGAT 2: 2208, 60612 }( ]8 ]0 |4 N
Csp45I TT/CGAA 2: 1110, 2447
2 o4 W$ \1 z9 S7 s# u8 z* G9 NCspI CG/GWCCG 1: 100763 Q8 }8 {" `$ X( c: b8 ?
CvnI CC/TNAGG 1: 2091
: v+ V, z* A7 u* z o7 C7 e. ]2 WDraI TTT/AAA 6: 2931, 6541, 8198, 8227, 8594, 8754; w6 O# ] M$ w7 k) Y
DraIII CACNNN/GTG 3: 2563, 9909, 10202) E# D6 e! Y2 ^, Q
DrdI GACNNNN/NNGTC 9: 2180, 2477, 6887, 7300, 9604, 9985, 10960, 11287, 119489 z7 Z7 D& y. w: l& g
EagI C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,
* P9 ^/ ~( | k9 u5 r9 j* o 5738, 5885, 5958, 6074, 6679, 9543, 10113, 10278
% `: h6 [; ]3 z1 `7 r% a T S; S7 OEcl136II GAG/CTC 1: 11556
; W- Z; n, d& \- r/ {3 x x% ]Eco31I GGTCTCN/ 2: 6384, 2463
) [- U# B5 m4 l* H' k. n: REco52I C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,# p. d; {' f; s& ?6 z
5738, 5885, 5958, 6074, 6679, 9543, 10113, 102786 D: O# e$ H% a
Eco56I G/CCGGC 16: 3301, 3475, 3525, 3529, 3556, 4055, 4413, 4730, 4870, 4959,
" }- P- S+ f1 } 5205, 5426, 5633, 5637, 5782, 6763) }1 ^# _! V2 Z. K
Eco57I CTGAAGNNNNNNNNNNNNNNNN/ 6: 1038, 2175, 4093, 5002, 7740, 11789! Y6 T" }+ B- P: J( ~
Eco72I CAC/GTG 2: 2150, 2563: k' s: W. }8 l" e* u
EcoICRI GAG/CTC 1: 115560 D) O% K, R t9 c( u& v' E
EcoNI CCTNN/NNNAGG 1: 62941 w+ L8 X3 }- _4 @! J9 S
EcoRI G/AATTC 1: 11548
# d: g# Z1 d, m, P( l' @: DEcoRV GAT/ATC 5: 574, 805, 8029, 10653, 122531 I" `% p: a8 w) x4 b
EheI GGC/GCC 1: 6768
! T9 {" S( G# D, h. iEspI GC/TNAGC 1: 8302
: u R' ~/ I. _7 WFseI GGCCGG/CC 3: 3533, 4059, 5637
" u6 l/ z' T, u! RHindIII A/AGCTT 1: 11599
, u3 o; s2 [: D; g1 b3 Y# _KpnI GGTAC/C 1: 115645 W* Z- F6 t) _
MfeI C/AATTG 3: 147, 2385, 11997* d' {7 ?( E) s" k" E, s
Mlu113I CC/GCGG 1: 8904
) e: }& Q9 h* b3 c" Q) h/ uMluI A/CGCGT 3: 612, 1310, 1328! b7 r; K4 b2 u4 ~4 v) x( F
MscI TGG/CCA 7: 638, 1996, 3364, 4912, 5179, 5646, 6592
5 C/ x) c8 U/ WMstI TGC/GCA 2: 6198, 117445 Y6 [0 J, l# y* R) h9 u
MstII CC/TNAGG 1: 2091
3 w- x5 E- M% `, Q5 U+ f0 ]! U& ANaeI GCC/GGC 16: 3303, 3477, 3527, 3531, 3558, 4057, 4415, 4732, 4872, 4961,
4 J$ s; B' y9 f( R( g; E. V" Q 5207, 5428, 5635, 5639, 5784, 6765
/ k! g6 y: m v7 T% v. }NarI GG/CGCC 1: 6767
) {, ]9 c) Z( W9 M1 r' VNdeI CA/TATG 4: 2055, 4526, 7015, 10035
; ]+ K6 P: E' b3 w( D3 SNheI G/CTAGC 2: 2540, 5981/ \1 b5 C. l1 \& m5 ^
NotI GC/GGCCGC 3: 3857, 5389, 6679 x; A/ i5 {: T% H; J5 t, |
NruI TCG/CGA 2: 90, 1734
( j j; _/ f- c- kNsiI ATGCA/T 2: 2715, 7918
( E1 Y/ A7 s2 X7 y+ SPflMI CCANNNN/NTGG 3: 2245, 4789, 5653% T" D5 m* Z: K1 i* Q1 D, Q
PinAI A/CCGGT 2: 6475, 10145
' _+ C) S! u, `8 R$ {PmaCI CAC/GTG 2: 2150, 25636 r; H7 Z. x& s9 \, ~# P% _
PstI CTGCA/G 1: 11591
" X L7 B" X. _1 e' G% @" KPvuI CGAT/CG 4: 2597, 9438, 10125, 117259 y" M; {# h) c! |: Y
PvuII CAG/CTG 4: 1090, 2501, 11372, 11694
6 v# E4 y: ~8 o3 n6 k) Z jRleAI CCCACANNNNNNNNNNNN/ 5: 2282, 6787, 59, 296, 5643) z2 b' N$ _ _5 h
SacI GAGCT/C 1: 11558
+ o3 K! i( R& d+ L# ZSacII CCGC/GG 1: 8906' h; n' E0 w/ X) ]
SalI G/TCGAC 1: 11581+ z6 H2 E$ A& i: A n/ j
SapI GCTCTTCN/ 2: 7076, 6601( Q3 s% l6 t; J4 H8 b7 a
SauI CC/TNAGG 1: 2091
2 L1 X7 f. \" X% c$ ~' X5 l% iScaI AGT/ACT 3: 3009, 9263, 9517! r) Q: K G0 y3 Z! c3 ?; F
SciI CTC/GAG 2: 9426, 10520: b- ~6 \+ n5 d4 V
SfiI GGCCNNNN/NGGCC 5: 3622, 4056, 4065, 5331, 5643) Y3 O9 m$ W2 V& J4 I
SgrAI CR/CCGGYG 1: 6763# ? Y! ?9 ^& E/ ?1 ~
SmaI CCC/GGG 2: 10503, 11566
' t% Z) B% F+ uSnaBI TAC/GTA 1: 401( H8 x+ N3 U" P3 J' I
SpeI A/CTAGT 1: 141 {% V6 X, d" T- G1 P
SphI GCATG/C 2: 2978, 11597' p9 X! `2 X1 C2 d. j/ p
SplI C/GTACG 1: 5865
) J2 Z- F: J: O9 q4 iSpoI TCG/CGA 2: 90, 1734( W& }6 i6 R6 Q1 C
SspI AAT/ATT 2: 1286, 7956- ~# _2 O: h8 b
SstI GAGCT/C 1: 11558
0 C/ N( |8 m: ^. F9 {. V0 h& e' jSstII CCGC/GG 1: 89068 m* I ^( d& H5 j( A
SunI C/GTACG 1: 5865$ n& G. O7 E3 Q" W
Tth111I GACN/NNGTC 4: 6939, 9066, 9982, 10426
a' D$ y. N* w% w# xVspI AT/TAAT 4: 965, 9238, 11364, 11423
) V5 l8 A, Y* P: e1 aXbaI T/CTAGA 1: 115758 G: I2 ?# k! k% r
XcmI CCANNNNN/NNNNTGG 4: 2567, 3385, 4540, 85582 k Y5 X1 k! t
XhoI C/TCGAG 2: 9424, 10518
5 d5 e3 n# E; r, {+ g4 v! ^XmaI C/CCGGG 2: 10501, 11564
) E1 M0 r ?9 u5 T/ l! YXmaIII C/GGCCG 18: 3299, 3527, 3531, 3857, 3961, 4335, 4411, 4872, 5389, 5635,. p; @0 b/ x% j% x2 H
5738, 5885, 5958, 6074, 6679, 9543, 10113, 10278
, w# o9 O9 j8 M# N7 m7 R. aXmnI GAANN/NNTTC 5: 9239, 10693, 11020, 11918, 12213, K0 ?. C+ W( T6 W4 L( b
XorII CGAT/CG 4: 2597, 9438, 10125, 11725
; F+ H. y2 Q' o, y9 ^3 J' g" j, e f$ U" a( U& Z5 ~0 [7 B7 K; b5 D
List by Site Order+ e5 p) `9 l; K8 ]& @5 z4 l) A$ x9 E
7 BglII 3527 Eco52I 5885 EagI 9909 DraIII
- ~1 q6 Z" x) l. V) ]14 SpeI 3529 Eco56I 5885 Eco52I 9910 ApaBI. X+ C! J# h0 A% S9 ^
59 RleAI 3531 NaeI 5885 XmaIII 9982 Tth111I
: q) w. c2 W1 k" ]1 W74 BsmI 3531 XmaIII 5958 XmaIII 9985 DrdI
" a& Q1 G/ g4 a& w( M90 SpoI 3531 Eco52I 5958 EagI 10035 NdeI" g# Q% f1 ?$ ^$ z
90 NruI 3531 EagI 5958 Eco52I 10076 CspI
4 R% A# @9 `+ |0 e" p r, G106 BclI 3533 FseI 5981 NheI 10096 BstD102I
2 v: ?# h( f+ S" c* _147 MfeI 3556 Eco56I 6061 ClaI 10113 EagI0 y( Y! d$ B/ a9 B
296 RleAI 3558 NaeI 6074 XmaIII 10113 XmaIII
! _, O8 K6 |( v- v401 SnaBI 3605 BstD102I 6074 Eco52I 10113 Eco52I
4 z! x) n; d) f5 Q* G574 EcoRV 3622 SfiI 6074 EagI 10125 XorII
; A: {( `+ k+ w: c612 MluI 3622 BglI 6198 MstI 10125 PvuI, F" C8 Z3 G7 ?
619 BspMI 3856 BglI 6229 BspMI 10145 PinAI
: C) d, t: y2 }* G638 BalI 3857 XmaIII 6294 EcoNI 10145 AgeI
# g+ e2 b4 ^- F, w' N638 MscI 3857 Eco52I 6384 Eco31I 10170 BspMI( s( W. u7 H! L( i$ Z0 x
805 EcoRV 3857 NotI 6475 AgeI 10186 ApaBI: s% G& P. o6 r6 F$ {9 B
859 AlwNI 3857 EagI 6475 PinAI 10202 DraIII
& r! d4 G/ t4 g, A& V901 BspHI 3961 EagI 6541 AhaIII 10203 ApaLI4 F" u' X+ G- I# Y
950 Alw44I 3961 XmaIII 6541 DraI 10203 Alw44I
- n, a: N+ e# @. z5 Z950 ApaLI 3961 Eco52I 6592 MscI 10262 AccIII6 N9 v5 `) K9 U0 k- z, Y
965 VspI 4044 BglI 6592 BalI 10262 BspMII
$ c' _' d4 i, r; O3 r1 j! j( ]9 _1038 Eco57I 4055 Eco56I 6601 SapI 10278 Eco52I t, X/ b: {3 I" T# z; E
1090 PvuII 4056 BglI 6679 EagI 10278 EagI
6 |& d. h [+ h! m1110 Csp45I 4056 SfiI 6679 NotI 10278 XmaIII
2 ~2 ]3 |$ X/ a" b1110 AsuII 4057 NaeI 6679 Eco52I 10383 BsiI- O) _0 f5 t, B8 |# c0 O/ D) |! B
1137 Bsp1407I 4059 FseI 6679 XmaIII 10426 Tth111I
4 ?6 ^, Q3 H- E5 t+ [& U; d1275 ApaLI 4065 BglI 6691 BstD102I 10464 AatII
* B( Q+ W" C r- Q+ {& o& m1275 Alw44I 4065 SfiI 6763 Eco56I 10501 XmaI2 o3 ]! R. T# _8 U; P
1286 SspI 4093 Eco57I 6763 SgrAI 10503 SmaI
. C6 V4 z. r' V! B5 N- v1310 MluI 4335 XmaIII 6765 NaeI 10518 XhoI+ l) i# D7 w7 G2 h# y: ]
1328 MluI 4335 Eco52I 6767 NarI 10520 SciI
9 d- s. B2 \" k. U9 P# F1349 BspMI 4335 EagI 6768 EheI 10653 EcoRV B& F' Y- M! L4 _8 ^& d; C
1563 Bsp1407I 4411 EagI 6770 BbeI 10683 Bsc91I
, n% l! T. B- @% s1734 SpoI 4411 XmaIII 6787 RleAI 10683 BbvII
* X, n- X5 W" U. d, E K1734 NruI 4411 Eco52I 6887 DrdI 10686 BbvII8 ]- j' t# m: r: M/ P" \% Q
1996 MscI 4413 Eco56I 6939 Tth111I 10686 Bsc91I
" S" {8 c7 P. q8 k1996 BalI 4415 NaeI 7008 Alw44I 10693 XmnI! k- @% H! i0 @6 N1 y O+ H
2055 NdeI 4526 NdeI 7008 ApaLI 10715 BsiI7 J) h$ f% ]1 i- B# I) r4 x; I
2091 CvnI 4540 XcmI 7015 NdeI 10911 Bsc91I, q5 N0 I( b$ X
2091 SauI 4721 AlwNI 7017 ApaBI 10911 BbvII
?: `' f+ v; i; D/ m1 I2091 Bsu36I 4730 Eco56I 7076 SapI 10960 DrdI" ~& q( U6 g+ C) R6 n e
2091 MstII 4732 NaeI 7125 BstD102I 11010 Bsc91I* u1 ^# L6 W8 ^4 t7 G% u) Q/ {. R
2150 Eco72I 4789 AlwNI 7300 DrdI 11010 BbvII
. n9 U( z X% I6 C- L2150 PmaCI 4789 PflMI 7365 BsiI 11013 BbvII
( V0 F. r( d$ K& p4 `: [( y0 n. Z- |2175 Eco57I 4870 Eco56I 7506 Alw44I 11013 Bsc91I- d8 i4 K" Y+ t v# j* W8 H' b: w
2180 DrdI 4872 EagI 7506 ApaLI 11020 XmnI$ G8 v; i. Y. B9 C+ `
2198 AflII 4872 Eco52I 7608 AlwNI 11042 BsiI6 F3 L+ R# c4 j" O" ?' w* H+ z* r
2208 ClaI 4872 XmaIII 7740 Eco57I 11238 Bsc91I
6 u8 X1 Y" k D7 a2245 PflMI 4872 NaeI 7916 BsmI 11238 BbvII
8 I. j4 g. G& ?2 q5 t8 g2282 RleAI 4906 AclI 7918 NsiI 11287 DrdI
; G( ?; h5 F: ]6 P4 N2346 BbvII 4912 MscI 7956 SspI 11305 BstXI4 l1 }6 J5 ~$ l
2346 Bsc91I 4912 BalI 8029 EcoRV 11364 VspI9 @0 a6 l" N) w5 }
2362 BclI 4918 BglI 8198 AhaIII 11372 PvuII/ `7 E8 F2 c0 c W0 {0 T; N
2385 MfeI 4959 Eco56I 8198 DraI 11423 VspI+ p! m9 c2 o6 I8 l: B1 k7 z0 H
2447 AsuII 4961 NaeI 8227 DraI 11504 BstD102I
0 ~/ B! O$ N3 g) s; @; x2447 Csp45I 5002 Eco57I 8227 AhaIII 11548 EcoRI
0 i0 O4 n# O |9 e! L0 t2463 Eco31I 5153 BsmI 8230 Bsc91I 11556 Ecl136II; V: i G& x( {' ?
2477 DrdI 5179 MscI 8230 BbvII 11556 EcoICRI
. t% b1 U5 m& `+ M6 [: g; j7 E2501 PvuII 5179 BalI 8302 Bpu1102I 11558 SacI8 N& ~/ e/ `0 c9 q9 [7 ^9 e
2504 AlwNI 5205 Eco56I 8302 EspI 11558 SstI
9 Q" g% a; w6 Q' f9 t w; g* }2540 NheI 5207 NaeI 8452 BspHI 11560 Asp718I" i. n& ?! }% S4 L# o
2563 DraIII 5331 SfiI 8558 XcmI 11560 Acc65I# T6 L% ~" E d% c% h
2563 PmaCI 5331 BglI 8594 DraI 11564 XmaI" b4 W5 c8 s! q3 _) Z8 t! d
2563 Eco72I 5389 XmaIII 8594 AhaIII 11564 KpnI
, l4 P: F% g7 _% U$ b2567 XcmI 5389 NotI 8754 DraI 11566 SmaI
) _" o) `, v0 T3 n2573 BstEII 5389 Eco52I 8754 AhaIII 11569 BamHI7 K; `* z8 u8 J' g. A2 X
2597 XorII 5389 EagI 8810 BsmI 11575 XbaI
( T6 `* K! v- ?. H# f2597 PvuI 5426 Eco56I 8904 Mlu113I 11581 SalI
2 B6 J7 R2 }5 X2624 AflII 5428 NaeI 8906 SstII 11591 PstI
% {' O5 |, {4 U! q2715 NsiI 5437 ApaBI 8906 SacII 11594 BspMI
3 d) `. ?7 r5 G' k* {2795 BssHII 5633 Eco56I 8909 BclI 11597 SphI! }, J+ a, {: c- F) U
2819 BssHII 5635 XmaIII 9036 ApaBI 11599 HindIII
5 l4 C" P9 ?5 f2931 AhaIII 5635 Eco52I 9066 Tth111I 11694 PvuII( w2 i( y) a0 s1 B
2931 DraI 5635 EagI 9238 VspI 11725 PvuI. _* G8 o3 A+ [+ h6 b- y
2978 SphI 5635 NaeI 9239 XmnI 11725 XorII
/ T$ u- Y" ~" E3009 ScaI 5637 Eco56I 9263 ScaI 11744 MstI
: G' U: \6 N1 q: h4 F0 b# `3062 Bsc91I 5637 FseI 9424 XhoI 11754 BglI
: x( X% t2 r8 {8 h2 y3062 BbvII 5639 NaeI 9426 SciI 11765 BsmI+ v; P4 e) U! n! M7 p
3236 BglI 5643 BglI 9438 PvuI 11789 Eco57I( b. E% ^) O1 o7 r0 N- L
3299 EagI 5643 RleAI 9438 XorII 11918 XmnI3 n: i- D# i' g9 K: D# W- ?+ j* o
3299 Eco52I 5643 SfiI 9517 ScaI 11948 DrdI
* @/ S. Z$ `6 B- Y4 }. j# R; @' \3299 XmaIII 5646 MscI 9543 EagI 11991 BbvII
( s: Q! l/ `2 ?! K, m& ]3301 Eco56I 5646 BalI 9543 XmaIII 11991 Bsc91I
( x' ~& R* x( r, p9 k! X' V3303 NaeI 5653 PflMI 9543 Eco52I 11997 MfeI i( X2 G8 H3 y( P, ]
3364 MscI 5738 XmaIII 9589 BspMII 12026 BspMII
1 \1 j+ Q8 ]/ k6 B3 Z b8 Y y- \3364 BalI 5738 EagI 9589 AccIII 12026 AccIII
2 ]: V, U9 W& j# K3385 XcmI 5738 Eco52I 9604 DrdI 12187 BsiI- F8 e( c% N2 L9 l
3475 Eco56I 5782 Eco56I 9725 AccIII 12213 XmnI
6 l4 a, l0 ]: h! r/ L1 z3477 NaeI 5784 NaeI 9725 BspMII 12216 BbvII
7 [( G( z1 d% i# x8 N8 j k3525 Eco56I 5830 BsmI 9738 BstD102I 12216 Bsc91I
3 Q ^2 y" h* {& C* O% e& K% J: {3527 NaeI 5835 BspMI 9901 Alw44I 12219 BbvII
$ C2 b$ n D- k# [8 E/ c3527 EagI 5865 SplI 9901 ApaLI 12219 Bsc91I$ n! R. ?, [/ ^, [' w
3527 XmaIII 5865 SunI 9904 BsiI 12253 EcoRV( L5 E) O [1 c0 m- I5 c
0 I/ i0 F9 t" j
2 U8 c0 n# O! U+ _1 MNon Cut Enzymes7 g* ]! c% M/ m$ v: `; L
ApaI AscI AvrII Eam1105I Eco47III HpaI/ q4 Y$ v2 W; r- c- t! [& Z
I-PpoI NcoI PacI PmeI SrfI StuI
, @' `& n* \+ N- v) SSwaI
