pCAMBIA-2300
LOCUS AF234315 8742 bp DNA circular SYN 24-APR-2000
+ K/ G+ h, v8 aDEFINITION Binary vector pCAMBIA-2300, complete sequence.2 Q8 x$ q) a1 Q! o6 B) M2 ?
ACCESSION AF234315' P9 N2 e$ i% W1 W
VERSION AF234315.1 GI:7638145/ u q0 v6 ]/ g) S( F' k- q7 a- c
KEYWORDS .& N" H# h* g" Q) q/ h6 Q5 E
SOURCE Binary vector pCAMBIA-2300; s6 J+ ^5 M" a; f R: P
ORGANISM Binary vector pCAMBIA-23009 ~' A( u% _( e( |8 v
other sequences; artificial sequences; vectors.: P+ [7 W5 }* F! ?7 f/ }- B: Q
REFERENCE 1 (sites)9 g; |) z- Y: T7 |( {
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. }1 d5 c4 J+ X* x5 Q4 B5 _# p
TITLE The small, versatile pPZP family of Agrobacterium binary vectors" o O3 A5 a; b2 g1 H4 t" e; P# R( E8 |
for plant transformation
) b. }% u5 S4 u8 n* V1 @ JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994). c# |3 ]3 H" W2 T# Q2 S8 h( B
PUBMED 7919218+ W. V9 f2 w) _0 ?: Y
REFERENCE 2 (bases 1 to 8742)
' ]0 N1 q$ v4 c, s: V AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
, w; |- j7 Q: A: t1 L4 A Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
) H8 r4 n9 O# k, ` Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
, A, a" A7 P9 T* I- S) k Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
7 m; [2 [' s" D TITLE A comprehensive set of modular vectors for advanced manipulations# z! R4 \4 P7 q3 @# W$ u, S# c0 U/ y
and efficient transformation of plants
8 M: C. e( d$ V! u# C9 l3 j JOURNAL Unpublished
9 d0 z0 O! F+ J, e- h REMARK Full description of constructs1 e6 f0 L4 {4 ~7 ]6 a: w
REFERENCE 3 (bases 1 to 8742)8 Q7 V8 `7 z5 u
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
. @' t7 ?2 i& I! ^/ D! ^ Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
" f9 |$ V: P/ W Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
/ x/ G$ o6 P, K: | Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
1 N- R0 y* J9 ^7 @5 ~9 ` TITLE Direct Submission
8 p( z0 [1 |6 H: i5 H3 v) o3 k9 ~4 k JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /, i @* e- t) q) Q& k3 t
GPO Box 3200, Canberra, ACT 2601, Australia
8 e; C9 n% C5 _" W9 h9 _FEATURES Location/Qualifiers1 I, X& j8 A) p9 m# h+ F
source 1..8742; }( A. O! j; ], z, M# u' F
/organism="Binary vector pCAMBIA-2300": G! H6 T G2 J/ k- R+ L
/mol_type="other DNA"6 e: n2 Z+ Q( H1 k K2 I0 n
/db_xref="taxon:118407"9 [. _2 i" U2 q6 T- Q
misc_feature complement(980..1980)
$ c+ L3 G7 E b5 J [& ?; { /note="STA region from pVS1 plasmid"
. ^, j9 h1 [5 o0 i+ @/ ^ rep_origin complement(2573..3573)' \9 Y; \* y5 e! ^
/note="pVS1-REP; replication origin from pVS1"
# a7 }* U! l. ~1 i; u p3 ^ misc_feature complement(3983..4243)
$ Y9 K( f3 ^6 X# a! k8 a# G6 k1 w1 b /note="bom site from pBR322"
; i8 `4 x, `" R: e rep_origin complement(4383..4663)) R* F8 t+ U& r4 H+ E$ n# |
/note="pBR322 origin of replication"
' i q7 G7 Z7 T: }7 D9 F! Q CDS complement(4954..5748)% ^5 [9 d& E+ Z7 s d
/note="aadA (kanamycin resistance) gene amplified from
% v5 z& D% D ^8 l' l/ G3 H4 x. f$ a pIG121Hm" R) |% d% N- {( f- n
/codon_start=1
! r7 p. i& I5 n8 W' l /product="aminoglycoside phosphotransferase"
0 R4 Y% B+ b/ {, Z) | /protein_id="AAF65399.1"3 b ~8 C4 N3 T
/db_xref="GI:7638147"$ I1 q) m7 s$ ~+ Q; L/ d$ y
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK2 e8 _" Y7 {* }
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
5 a! i K- c/ o) _" X EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
u( Q, X) l G7 G! k& y EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW i) G9 q+ d; Z6 F* R/ c
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF". O) W6 c1 z- G% x) r( ~/ y% _9 r
misc_feature 6173..6198
0 ~$ T4 C9 L- h1 @8 k' G- q. u# b /note="left border repeat from C58 T-DNA"
) q/ e1 R/ {5 N. H misc_feature 6265..6468
4 L3 m @+ ~, y4 ^( Q /note="CaMV 3'UTR (polyA signal)"
9 ^/ y6 D% ?, F CDS complement(6506..7303)
6 L6 j/ T% U! H% b- n /note="nptII (kanamycin resistance) gene"* Q8 I& s( \) I5 l9 k7 ?
/codon_start=1* t6 f3 [& _+ b1 |* i1 u( u! Z
/product="neomycin phosphotransferase"2 H' ~) m( L! z/ Y
/protein_id="AAF65400.1"
1 S5 ~# }* P. u& V5 V" a* P /db_xref="GI:7638148"& T5 \ \4 m: Q/ j" I
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
1 `! I0 m" G6 ], Y RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
& c( v. }# |! p LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD4 x a) R, C* C6 \/ @8 ]+ V
EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
@5 {6 i, \9 k [ YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"' ]# c& ?1 @' p5 H
promoter complement(7334..8114)" x0 Y4 X$ o5 ~
/note="CaMV35S2; CaMV 35S promoter, duplicated"/ y8 O, E2 x) U) z4 `
promoter 8262..8316# X' W; k1 E y9 Q4 K
/note="PlacZ; lacZ promoter"
9 r, ~8 ? h; ^* B5 v CDS 8344..85777 ^6 T; \$ ^4 ]' s* X
/codon_start=13 k" h' ?. Y0 p
/product="LacZ alpha fragment"
3 v6 y- `% L$ M2 \3 T$ A /protein_id="AAF65398.1"
( D% Q5 L. W# E$ J8 U. a, D /db_xref="GI:7638146"
, q! ^2 D6 _$ s- W /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
& ~0 `7 F4 W6 @6 |) ], I RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"! c& ?- V+ i' k! H6 g8 s% H* C& @: O
misc_feature 8358..8414* _4 F( d0 Y$ O1 A
/note="pUC18 MCS; polylinker" q6 F0 x( K0 A% S* ^
misc_feature 8656..86813 O) ~/ Q# w q1 u2 S4 B
/note="right border T-DNA repeat", D1 W% W" X, o. q# F( S
ORIGIN 5 `% R# P$ \( g' d
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct* e$ `' w# ?% R3 Z. y) z, Z7 [
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca9 W5 H5 \) J& k, `4 `& e: R% w
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt; C6 u) M$ O, a- x
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
2 W) x' ] Y9 e: Z' `1 D 241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
% ?/ _: Z- g# x' z9 k 301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
) Y0 H0 D P) x: c 361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg" B( w" ~5 b Q x7 s
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
% `6 S/ e+ Y, U 481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg9 q* h: P4 d9 X, r4 \2 G1 s5 V
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg; e9 I; H, [" v3 L2 Q
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
# Y6 z: E. R. u- U J% z6 D 661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga, L& v( S: v. j4 \9 m
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
' K) L, v8 x. k- F+ V' A 781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
4 \$ ?7 ?7 t+ T' Z$ N, f 841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
7 m! n" |8 J5 C% E' z 901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac& m4 g5 W+ |1 z# M' [8 f; V; M
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt% j; I) t' H& ]. G
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg3 }) B7 F8 q( I4 u) V3 ~
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
" g0 W* ^& f" v" K/ z( | 1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
) y4 l) L7 ~% \! }- P$ a9 l( f$ ?. J 1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
3 l2 {0 h9 o+ p- v4 R 1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg* u+ t. [0 F, ]6 }! o) J
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
# Z; s7 x& J1 u5 ~ 1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
7 k4 O9 Q7 o5 s. i! w- p 1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
- g' l/ ?% J% G; r 1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
) @& d2 s+ W) q& u2 w6 ? 1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa- R- \: V j9 A6 M" ~; G$ }: ~0 E
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
# i: `) z7 d9 C" }5 \% x; l 1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
* A! H# a9 M2 B2 E) U4 ? 1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc6 y; B \% I, R) X* q% S
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta2 |5 }3 b& P) C2 Y0 `
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
4 g6 @: P& f: h 1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc5 G d: h& X1 y& }1 G6 k
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
# W$ _" x- v3 o2 w5 m( r$ `2 { 2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa5 h' k2 d( ]) t- t E, b5 P2 F6 Y# W
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc, z9 o+ u7 z0 s" E: p9 \: J, H: @
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
3 A6 \! ~5 y; L7 C& e4 V3 {# o 2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
- e+ a. A9 k7 |) M. P% Q: I 2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
6 b8 k! G0 a& g3 ~ 2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
6 D( q( b9 x% p- |- w 2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc: W6 y2 Z3 q+ i* `
2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc0 p% K1 J" m8 o% `9 ? ?
2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
* l {5 i% o1 L6 s 2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
9 c6 k. L" s) R( H/ Y5 v 2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
1 d; @% y' @0 @) x4 ` 2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa- T" E" Z* N8 X. u; O( U$ t6 }
2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
0 H% |0 `+ U. f/ y2 n9 P6 P4 b 2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
6 {$ {" a- r* H6 L2 c% E1 P 2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga" m' Y9 J/ `# W$ Q$ L) z r
2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga1 r' C! a# I3 i7 I
3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct& \ A. f. n# f3 R
3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
- r \+ Q# N) N# u' ?$ [, H7 Q1 m# V 3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg3 h# _+ K& f% k
3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc/ q8 r$ O9 z3 g' D9 v
3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
8 T$ o% T b/ {2 A5 F+ q 3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
( f) z3 t2 A2 e! T2 j; R# d) T 3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga$ Y6 t8 @8 e0 \% B
3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
, d% `' I1 t- K0 B 3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa+ b* }3 p- Z2 h
3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
6 h8 X6 a8 _; i) ~ 3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg) H E, N% ]; G0 X
3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
3 j; C$ @4 P( e3 G9 r) o# b4 R 3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc" J* W/ j7 {; y7 r! C' A
3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg8 @7 u* V. V' p; E/ L7 d
3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
- n4 T- k- L' \$ ~ 3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
, W7 S' \1 T5 M8 N/ ~1 J% F 3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
* }( ^0 U1 D6 {8 S: ~8 G 4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa3 o) I/ l& f# r$ M6 u1 s
4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg C1 V2 u- ]4 s3 O, n- {$ T* Q5 @
4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg6 d6 {2 F# h, J* ^- U3 D
4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa4 V/ s+ H' ?" Q& @6 W
4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg+ Z& e$ A( i, S3 s; P" \2 d/ s1 l
4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc |% U$ \# t. o" M+ h& ], N" C
4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc6 ?. t+ ~; }4 S1 R& |
4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc4 Y+ h# `: u. z2 Y% v( c7 a* H
4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg, d5 c" @7 a+ c% I3 H
4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
: o% ]6 R3 X' p8 q 4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
- ^ n5 x$ Q4 |8 ? 4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
* C! P% I# l7 p/ B 4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
* d v$ p& j- f5 ]' C4 ~$ I) V 4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
* P) K5 H7 @8 z4 B 4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc, e: `: d3 U+ w
4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
8 Z, U' A: `8 U! ?9 i 4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
$ Q e/ a5 D$ @7 }( A 5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
# X. t. D$ f9 w" F! E% C; c3 I 5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
! C/ _& N7 @4 p0 R( X+ e" L- G( S 5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
6 p" l' U: R( u$ X 5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc; {; L6 _! S* P5 M n
5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
9 N$ H# _3 J4 ~4 W3 |7 z0 k 5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
8 w4 T5 u8 T3 I- w$ `% v2 G8 N 5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag1 j3 _3 E5 j2 U5 k; R
5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
$ v/ O- H: w; m 5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac3 g, U6 q5 }4 I2 b8 q& D `/ ?
5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
$ m; N( ^4 N' ]& [2 C& y 5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta! r1 k9 _& h% n
5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
- B, G% Q8 r; _( Q! B) O9 u9 i" T 5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
3 J7 Q X9 t+ U; |! ?: @- g# p 5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
3 R+ e5 M) A v& s/ N- E 5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca' ~' X: l* M* t) a# c: g+ o' G/ X
5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
" I5 t+ d& x% [5 c 6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag5 @4 q( [8 y" i2 `7 ~
6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
' c# i3 {; k* J6 m 6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
# i0 b( Q$ f) a8 ^% z3 @$ g! f 6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt0 g( B1 P- c" q. ?1 X
6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg8 r$ H+ E0 U/ U" B
6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag8 k& }$ R1 K# b0 b* u
6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg: D- \( }& j! v7 a& p, W, J/ h
6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
# l# j2 G* J+ W. \0 D4 y 6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
, y J* y: D# R4 r 6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc7 `- h9 k5 C- M7 }
6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca- I/ C2 d) K4 ]9 T6 V
6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
+ W( ^4 J6 f- R. T 6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga1 M! e4 H' A1 n6 i
6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
' b1 z/ v5 x$ h9 l 6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
: R+ u2 a \. ]: a* D0 ~4 M2 t j9 G 6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
( K, O; c1 H9 \ K 6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
' k! Z- S; b8 k% ~ 7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc" [0 g1 I8 u% P# G/ X9 N. c2 e
7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
! S6 t& c- ~: s9 y' Y; o/ a 7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg3 y/ \6 Y9 |6 f8 h; C3 m& n
7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
0 ]8 h# Y1 [5 m0 C5 X+ C 7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct$ ~$ @% K, a' Q
7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
: B2 i; _2 t, e% k 7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
4 S5 z2 b/ @; J' b: ]+ A/ B0 \ 7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga) S# F5 X6 P0 e; I0 t# L
7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg+ y- G& f8 Y2 ^3 l. a9 j5 _7 Z; p
7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
/ s- L# Q7 c2 R 7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
' E' A# G4 S% J# S/ r+ y 7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
* r! d4 m! q* m/ n' k. G2 X2 o 7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa/ b: c% R$ F; ]6 U
7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt) J0 ^: a$ y( f: ^! _" a/ i
7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta D6 N+ F* h! w7 ^
7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
! M: M5 n8 L0 x9 ~$ o- D9 W: _ 7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
5 I5 @# P% ]2 |5 [" z- i0 Z T) C 8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
0 A. l% q9 z( h9 h/ n! N 8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc. N( l5 y( x% ~1 w* n7 x! \- d. s, x
8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg. d% A* z# o- A. p8 i( f, g! E0 V1 W
8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
/ p: j6 }$ s* p 8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
$ }# i$ K# {' v3 l/ y$ w 8341 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc
4 \8 d4 a! Z2 n; s F: r8 } 8401 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc+ U8 M1 V! @/ m. W1 P# g3 J0 T
8461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa4 ]# H1 S" m8 P
8521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc1 x- i) R1 a- U
8581 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
5 l3 p( N3 ^1 y, j9 C' v4 I9 M @4 H 8641 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta0 }+ @- z, B. i9 _0 } q
8701 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tg
* ?3 |6 i( Q3 r' o* _: s v5 }//
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