pCAMBIA-1305.2
LOCUS AF354046 11921 bp DNA circular SYN 16-APR-2001
6 T/ b4 E, x; C% H& o/ q' p. NDEFINITION Binary vector pCAMBIA-1305.2, complete sequence.+ v6 i; K/ S. c+ C0 p
ACCESSION AF354046, q; y; \& J. x
VERSION AF354046.1 GI:13540351
s. V9 w. u6 N6 s P/ a" n2 w6 ?3 LKEYWORDS .
A9 W, Z% c8 z8 Y. n/ U( ZSOURCE Binary vector pCAMBIA-1305.2; F# r2 |( v1 [
ORGANISM Binary vector pCAMBIA-1305.2) \7 \3 J6 s+ S- `$ T( I- a
other sequences; artificial sequences; vectors.
: Z j# H; h. d' v# e$ ^REFERENCE 1 (bases 1 to 11921)
1 o; @/ R5 \( m, L$ k AUTHORS Jefferson,R.A., Kilian,A. and Keese,P.K.$ R; q- ^8 d9 Q) l
TITLE Microbial genes for secreted beta-glucuronidases, gene products and
- z+ C8 ?) p( c% K& C; w uses thereof, r# n& U9 I' G" V
JOURNAL Patent: PCT (PCT/US98/19217)-A 09-SEP-1998;6 ~9 _3 G1 M! \6 @5 P8 c0 t1 r. l
CAMBIA; Molecular Technologies; Canberra;
! U. \6 L8 a% O9 M$ H& v$ ?: O Australia;
! g) [( j- O8 W* h+ \# F/ t& E* K, ~8 L0 X: yREFERENCE 2 (bases 1 to 11921)
0 K. n/ [) N0 R" a/ O I+ l AUTHORS Nguyen,T.A. and Jefferson,R.A.3 Y7 G5 v& Y* e) ~! `
TITLE Binary pCAMBIA-1305.2
! f( U0 s9 d- p# c: [ JOURNAL Unpublished/ g% ?7 C8 d3 h: F- g
REFERENCE 3 (bases 1 to 11921)
% g0 S' R% z6 h* H( v# N- c AUTHORS Nguyen,T.A. and Jefferson,R.A.& }+ t, g, `: t& _
TITLE Direct Submission
& t! P; `3 w! n1 O$ g1 t JOURNAL Submitted (13-FEB-2001) Molecular Technologies, CAMBIA, GPO Box
" `* t. I" X+ P( e) V& o 3200, Canberra, ACT 2601, Australia
~, x2 D1 a: [. |* ]4 C JFEATURES Location/Qualifiers
: N* s' _' U" ^( m# d9 D# W source 1..11921; {/ b+ c6 t$ g, i# W
/organism="Binary vector pCAMBIA-1305.2"
& i* ^4 z3 ?! q9 M; G /mol_type="genomic DNA", p" |! d3 @: T4 F; Q( @* R3 j) T
/db_xref="taxon:156493"! v, S+ z$ C$ O6 d3 Z; e2 ]
CDS join(2..91,282..2129)
" S: {" k; m6 s9 H6 A9 }; g( h# N5 H; f( W /note="GRP-GusPlus-His6; GRP signal peptide from 2-81
6 w/ |" H- y' f( U& c (glycine-rich protein) for secretion of GUSPlus-His6"
" i4 k6 G) Q1 t/ { /codon_start=1
7 V: T& c; ]0 c/ G( W4 o& R$ v* d /transl_table=113 i( e7 M @8 u& c8 C9 w
/product="beta-glucuronidase"
3 z( y2 F1 y% R+ Z3 j2 X /protein_id="AAK29427.1"5 m! M% n: S4 F# g0 I3 K
/db_xref="GI:13540352"8 a8 I+ K6 ^+ j7 a
/translation="MATTKHLALAILVLLSIGMTTSARTLLDLRNRRTSLYPINTETR
% j W6 ]- X$ G+ Y+ R GVFDLNGVWNFKLDYGKGLEEKWYESKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWY0 j) `! S4 D6 Y+ W0 d
EREFTVPAYLKDQRIVLRFGSATHKAIVYVNGELVVEHKGGFLPFEAEINNSLRDGMN
! J; l: x5 U; I RVTVAVDNILDDSTLPVGLYSERHEEGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFT
1 b @4 R# R, O! [3 v$ K YVEDISVVTDFNGPTGTVTYTVDFQGKAETVKVSVVDEEGKVVASTEGLSGNVEIPNV
8 D& h4 O+ C7 P* ~. { ILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKH
9 b2 p/ D: X' I EDTPINGRGFNEASNVMDFNILKWIGANSFRTAHYPYSEELMRLADREGLVVIDETPA G i& r6 C5 D3 e) n
VGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDVLRELVSRDKNHPSVVMWSIANEA
8 a9 z& G8 n1 n, {1 C ATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMATPETDKVAELIDVIALNRYNGW
6 x$ I/ d0 @0 g a YFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGADTVAGFHDIDPVMFTEEYQVE6 N: u" {$ F1 c1 q `
YYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRE$ \ }7 g( j9 O' A0 t& i
RWTNIPDFGYKNASHHHHHHV"
7 [0 R- I9 g( A2 u" S, [ intron 92..281; I/ S$ O( B# g
/note="catalase intron"
' D- m% Q" w5 ? polyA_signal 2158..2410
1 D* r8 ~, p V: s H: ? /note="poly(A) signal from nopaline synthase"0 g/ Y# C3 |0 K2 e( [5 _3 v7 Y$ z7 r
misc_feature 2429..2453- @1 d, ^$ n9 V+ V
/note="T-DNA right border": u- Y c2 D: W' \; O% @6 U
misc_feature 3514..4514
$ {7 ]4 J0 e: q; W /note="sta region from pVS1 plasmid"4 {3 a& i O. m4 @- H1 z5 E
rep_origin 5107..6107
; w- c; Q$ B* k) p /note="pVS1 rep"
1 m4 t5 }! F! V, w/ `' V misc_feature 6517..67775 e0 L0 l; e$ z* ~4 D* C1 C
/note="pBR322 bom site" F# z1 Q) [# v* o
rep_origin 6917..7197- S6 R- u3 d& p1 D4 i
/note="pBR322 ori"0 }3 m$ U+ e0 z, A- s
CDS complement(7488..8282)
" }; k/ J% E7 ]: d /note="kanamycin resistance gene; npt II gene amplified* n$ \2 r- @4 b5 y. H
from pIG121Hm; NPT II"/ J% @& H) g+ C, i) r' c, w
/codon_start=1. M; m- t( Y( m8 q# ?
/transl_table=11
7 Y+ ~" `5 `* c3 m+ x- c* V. N/ E0 ^ /product="neomycin phosphotransferase" G9 f; Y/ I* T% M8 t4 _
/protein_id="AAK29428.1". U* v( R. s9 ?. T
/db_xref="GI:13540353"
1 A# B# r4 e' B& `) B: R3 w /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK3 z, f) S; n, H/ l
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
/ Y# ~, H, Y7 u( t- E EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE4 A5 Z7 G4 T1 @+ U k$ a
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
) I8 ^* U5 C2 u! z! m7 A+ k YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
! H' t& o+ K; M; F) d9 [ misc_feature 8707..8732: w o, o' S k) I
/note="T-DNA left border"' T8 m! w; c9 {1 [
polyA_site 8873..88807 A1 T% s+ }! w1 T* O1 G3 D( [$ q
CDS complement(9023..10048) ?( O: Y# r6 t+ p& v# r+ X
/note="HPT II"/ X. ]' `+ \- H4 `+ f. N$ R& e0 [
/codon_start=1
* i1 _* Q. q' h; ^ /transl_table=11
$ f& y- g. x- \0 |2 P; |8 H /product="hygromycin phosphotransferase"
/ E1 b8 d6 H# K: B( E7 i /protein_id="AAK29429.1"+ w' a Y& A0 B/ g; o
/db_xref="GI:13540354"! s; Z( g. h) ]# E# \
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
! d0 K) J) |+ u) ?0 m GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD% c4 I6 _6 G* h8 U
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
$ n2 u$ L" N$ m3 q* f( x- U& S' m HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS: S8 z2 b1 f$ Y. f& m- {& G: E M
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
5 {1 @( z( {) x: X2 [ D$ J( s4 ? SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS. e* L% x+ N' E( j+ o$ _% Q% y
TRPRAKK" H/ `! I/ L' \5 Z; L* ^/ b
promoter complement(10891..11084)1 B8 ?- X6 O; n) e# W
/note="CaMV35Sx2; CaMV35S eukaryotic promoter with
/ _9 {- n: |& p: X$ W% b duplicated enhancer region"7 `% f% {' ?* n
promoter 11013..110905 y; D- e. X: j. i# A) I0 [' b
/note="PlacZ prokaryotic promoter"
6 `( o6 { l1 ` CDS 11094..11327; \6 t9 L- F- G
/note="lacZ alpha"
9 e @* o$ c, |1 t /codon_start=1, b$ \4 r/ U% L. y9 `$ F
/transl_table=11& A% S' E0 N3 P, c7 S/ Q& P
/product="beta-galactosidase alpha fragment", |( A, F3 a* n3 i8 f, t, @
/protein_id="AAK29430.1"0 H$ W( E3 S0 E+ w3 i
/db_xref="GI:13540355"
: R/ A5 C4 O/ p! x$ ]6 d( t6 Y /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN* V; {# n& \& x& Y
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
' K+ f2 s# L' R' c: @2 E" u3 @ misc_feature 11108..11164
) G! j. g/ b* W2 q) G2 E) ]( b2 Y w /note="pUC18 polylinker multiple cloning site"7 ^; e5 H: r" e' b
promoter 11378..11915# Q6 Q* {/ k4 k1 W
/note="CaMV35S eukaryotic promoter"* H1 G. r+ O2 u
ORIGIN
4 A6 e0 [; W& h" a& I6 n) Z+ a 1 catggctact actaagcatt tggctcttgc catccttgtc ctccttagca ttggtatgac
% |( I2 T+ ^( P7 b; V$ i 61 caccagtgca agaaccctcc tagatctgag ggtaaatttc tagtttttct ccttcatttt* r, Y" w2 ~$ I6 v
121 cttggttagg acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat3 |" `& I q0 j% ~8 [7 C
181 ctatttttta attgattggt tatggtgtaa atattacata gctttaactg ataatctgat4 A7 Y. r; z* _# S; A) P/ ~; k0 R
241 tactttattt cgtgtgtcta tgatgatgat gatagttaca gaaccgacga actagtctgt* S( ]6 t& I' R- n8 ]
301 acccgatcaa caccgagacc cgtggcgtct tcgacctcaa tggcgtctgg aacttcaagc' Q8 R" m/ c/ V. q3 F
361 tggactacgg gaaaggactg gaagagaagt ggtacgaaag caagctgacc gacactatta
- `+ a X4 a! l0 q" _7 [' ]+ X 421 gtatggccgt cccaagcagt tacaatgaca ttggcgtgac caaggaaatc cgcaaccata T) G1 O1 M3 e0 B. f- N' ?
481 tcggatatgt ctggtacgaa cgtgagttca cggtgccggc ctatctgaag gatcagcgta3 @' }/ f( L8 ^$ N `, k
541 tcgtgctccg cttcggctct gcaactcaca aagcaattgt ctatgtcaat ggtgagctgg
6 U D' b1 |4 a* x, {0 a 601 tcgtggagca caagggcgga ttcctgccat tcgaagcgga aatcaacaac tcgctgcgtg
& C5 I0 m# T4 R t 661 atggcatgaa tcgcgtcacc gtcgccgtgg acaacatcct cgacgatagc accctcccgg! ?2 w% r# _' b$ H2 r( \8 J
721 tggggctgta cagcgagcgc cacgaagagg gcctcggaaa agtcattcgt aacaagccga
1 j3 {5 t9 Q( E+ q2 K& F! d 781 acttcgactt cttcaactat gcaggcctgc accgtccggt gaaaatctac acgaccccgt
5 n, K/ @7 G+ N 841 ttacgtacgt cgaggacatc tcggttgtga ccgacttcaa tggcccaacc gggactgtga% l8 M4 T- P" B2 y; N
901 cctatacggt ggactttcaa ggcaaagccg agaccgtgaa agtgtcggtc gtggatgagg
7 P: w$ y) d. O$ {! } 961 aaggcaaagt ggtcgcaagc accgagggcc tgagcggtaa cgtggagatt ccgaatgtca
! f8 D8 `, C5 t( F1 d8 M4 S 1021 tcctctggga accactgaac acgtatctct accagatcaa agtggaactg gtgaacgacg' e1 ^. ?! P2 S/ Z( S: t
1081 gactgaccat cgatgtctat gaagagccgt tcggcgtgcg gaccgtggaa gtcaacgacg! E6 u( d' |/ N4 [, ?9 v; h+ ?% b! X
1141 gcaagttcct catcaacaac aaaccgttct acttcaaggg ctttggcaaa catgaggaca
7 b; V4 |. [/ w7 x l/ ? 1201 ctcctatcaa cggccgtggc tttaacgaag cgagcaatgt gatggatttc aatatcctca: ~/ z% |( t! ^! j/ u+ {
1261 aatggatcgg cgccaacagc ttccggaccg cacactatcc gtactctgaa gagttgatgc
4 f: @# u& t d1 O3 H3 _ 1321 gtcttgcgga tcgcgagggt ctggtcgtga tcgacgagac tccggcagtt ggcgtgcacc
8 ~9 v. K& Q7 O- T D6 h1 M 1381 tcaacttcat ggccaccacg ggactcggcg aaggcagcga gcgcgtcagt acctgggaga
, l, _% i$ l3 R" O 1441 agattcggac gtttgagcac catcaagacg ttctccgtga actggtgtct cgtgacaaga
( B& ^- |5 R2 m# V) j5 z; n 1501 accatccaag cgtcgtgatg tggagcatcg ccaacgaggc ggcgactgag gaagagggcg
1 g; V2 \2 X+ v+ D1 U' B a* F 1561 cgtacgagta cttcaagccg ttggtggagc tgaccaagga actcgaccca cagaagcgtc3 A3 f2 q; ]: d3 n# x7 P
1621 cggtcacgat cgtgctgttt gtgatggcta ccccggagac ggacaaagtc gccgaactga
1 Q, v1 j; P: c; P, d5 Y 1681 ttgacgtcat cgcgctcaat cgctataacg gatggtactt cgatggcggt gatctcgaag
7 h7 [- R! ?! K1 k( i 1741 cggccaaagt ccatctccgc caggaatttc acgcgtggaa caagcgttgc ccaggaaagc+ I5 I: _* A' ^2 c% U
1801 cgatcatgat cactgagtac ggcgcagaca ccgttgcggg ctttcacgac attgatccag
3 u7 N4 Q# p3 L" d& c1 a3 [6 Q1 t 1861 tgatgttcac cgaggaatat caagtcgagt actaccaggc gaaccacgtc gtgttcgatg
1 ?9 |' t$ W+ c( z0 C 1921 agtttgagaa cttcgtgggt gagcaagcgt ggaacttcgc ggacttcgcg acctctcagg
/ u, [, M0 ^" h: F, J 1981 gcgtgatgcg cgtccaagga aacaagaagg gcgtgttcac tcgtgaccgc aagccgaagc
9 W7 X" A5 z: ]5 i- S 2041 tcgccgcgca cgtctttcgc gagcgctgga ccaacattcc agatttcggc tacaagaacg/ e7 ?# T2 I* g1 ^+ W3 ~
2101 ctagccatca ccatcaccat cacgtgtgaa ttggtgacca gctcgaattt ccccgatcgt9 O+ U( c6 O( O6 } A
2161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt$ k0 M: G& J3 ^( m5 @
2221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg% U; _7 F* _& g, S. H* N/ X& Y
2281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata/ `% _6 R7 K$ B( G- |4 _/ K
2341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta6 O/ l& G% G% v! |! ~" u( B
2401 ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
9 j! X$ k, r! Z( ^3 N- V 2461 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg/ k/ y, n) \/ z+ Q2 J
2521 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
% U$ ?+ U+ D; {% T0 X# [4 N 2581 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa# U# |# H- O O: } i3 E9 N
2641 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
2 V% G% P5 T2 c: X- [ 2701 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
' V4 x0 @; I1 i q 2761 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
5 i% G( z* O2 P' | 2821 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
1 k: ~' b' ~* U4 b 2881 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
9 w! ^2 C! J: }; L, m0 r' } 2941 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg% k. d7 O" D! H
3001 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
, b+ G( p$ h: H* k% F 3061 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
/ r; p( B3 Z' s& ^; J( E 3121 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
+ u5 V* h: ?8 f* F W$ p0 T; B 3181 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
! b, m+ L5 j6 U4 Q4 L- X 3241 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg, M2 B8 w6 t) H; m0 F& E: Y7 Y- y, `9 U
3301 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga% u) p0 y: B( r9 E) F
3361 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
# _& H7 g3 c- o3 ^( \ h- c 3421 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
' l6 X- n2 z y. e; X+ E5 i 3481 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
% s: z N! @, g+ Z9 P# \# k0 \ 3541 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc0 S2 |9 }4 `$ }$ M6 i
3601 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa4 J) ~. {5 b+ e4 W" E4 X: n
3661 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
2 ?6 \ B( S) s5 w6 Z 3721 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga$ H+ Y R1 g6 s5 B/ y" S1 o' H/ G
3781 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
- c0 A4 H0 \! K 3841 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg" q' L; m; `& f7 g/ q( a+ M
3901 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg5 C1 W- P0 w$ x" |8 K1 K- V
3961 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact) W; W- U0 Y- m; e" [
4021 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
9 q$ g9 u- p# f% ` 4081 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg6 E0 U( c. H* p! @+ E- C
4141 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg* ]! d D" H+ d% ~ ~/ C
4201 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc8 G: d! K7 Y( L
4261 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg0 a: o& O+ D) Q q
4321 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt9 V, J- ]) X4 Y
4381 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
( p6 Z# A( H( _5 A 4441 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa% V6 m% H7 i" G7 q
4501 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
3 d! u) G6 z1 E+ T9 z' h 4561 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
; o# W$ k' M3 l1 ]5 l 4621 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
% ?# Q$ ]$ _# I- u8 f6 t' X0 N 4681 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc) {# e' k2 p( _7 T6 C9 ~
4741 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
* e% t4 k' S+ A7 E/ _# L% @ 4801 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt5 F1 x# t) F& @- n k
4861 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca1 P% n$ n$ C J+ R7 i
4921 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg' r9 ]& s! e @1 Q
4981 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca- X. C) n. Y9 J/ j% ^
5041 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac6 K2 M9 n& `# v
5101 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
# y z9 w) @6 F0 L; _ 5161 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
& ?& x' \: ?" |( \' ` 5221 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
! Z; G2 I+ O& j( V 5281 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc0 [9 g F0 C1 J9 P. T
5341 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta l* ]: q( \: N
5401 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg4 g+ X# l+ D9 ^ C$ S+ |1 n# X, ^; B
5461 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg* `& }# K$ u8 b! ~$ M
5521 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag' B) K, p4 y5 T5 F7 n1 D) A8 }
5581 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
: v6 k5 G' [) F2 x' n 5641 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag3 T2 a% v7 o5 ~# K+ V. l
5701 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc' h$ O6 [1 A$ @8 L8 J2 g5 N0 |( Q
5761 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
% z" H( k: \, H5 a" z 5821 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc, ^( u+ R, M; I: f3 _/ z# W! X
5881 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt$ _! T/ W: N5 g# @
5941 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac9 t' d9 V, x* Y& \7 c3 l
6001 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat+ Z. Z) |! a" Y9 o4 N9 S4 d: V
6061 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt- F- h/ c* [7 }: L; R* S# B, a
6121 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa6 B+ n0 N3 t; v$ [) |
6181 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg4 \5 ~! [! M' S/ F3 {& ~% ^+ J2 \
6241 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
' [' ~& `+ n$ N+ F" k' t 6301 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
6 y; i- n! f4 v$ D/ O, j+ E 6361 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
7 Y/ p" h# U& Y/ l# a 6421 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
+ J! N1 F& e, E 6481 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
/ u2 v# B! s3 H; h; c 6541 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
5 d1 Z) ^+ U1 Y8 @ 6601 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
* H6 ]. c( F' z' p1 O! r' O+ S 6661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
# D$ e/ ^) i O6 _4 f* P 6721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc5 {8 k! |1 ?0 ]+ v7 Z+ v
6781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
+ F( s0 _, i( x" u 6841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
, r! {8 @) q& g* E 6901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc, `: ~. a# n% z) A5 v3 V4 u; k7 }
6961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt8 D0 C; h, y/ D
7021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
- x0 Q0 w% p1 `; k9 b 7081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga: }( U' q* }- e9 a% X" l
7141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta: v! N) b3 k1 ^2 l I% |# r
7201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
& ^0 M2 Q# P; c 7261 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga/ u; u- E. W) T! V7 B, K0 K6 [
7321 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg- S' K3 t9 Z }" \$ p5 w# f C
7381 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag9 b0 f# |2 K, ]" t
7441 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca! c6 V) }8 Y) \6 H% ?
7501 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa7 k8 W# U/ z) t9 a3 B
7561 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
: x6 X1 z7 g7 o 7621 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
$ ^& k! N: d5 q0 ] 7681 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
) j9 J) |. f' X" y 7741 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
6 \% b' s+ a& }- Q# g3 s" j+ b. U 7801 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg- t# U @& D" } ?
7861 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag) s$ }$ P9 p0 [% W
7921 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
3 x9 B+ w* Y! x8 a' ? 7981 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc ^9 V# T, l1 ]9 a0 @
8041 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
; W& J% q: k, O 8101 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
d8 i# z. C; I1 c7 W0 {& x% I4 | 8161 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct( H+ _+ T8 D1 n! O! V8 N& J
8221 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc& x1 e* _3 N! `0 q
8281 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata* y# H @8 y9 P1 b$ I' D1 Y
8341 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
5 h. y1 u) W' I 8401 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga5 O$ `, ]5 V5 j
8461 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
2 I, X/ O$ L2 ]% \; p4 ~ 8521 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
; b( i4 |/ [. O! J3 a 8581 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
# `; n4 c% J8 ~8 ^) e4 X; X 8641 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
( P) j& o H; Z1 }1 r9 f 8701 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac% I7 }' a2 r0 g( X' d$ r- ?9 ?
8761 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt
4 c' X' C8 U( O/ Q% g 8821 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
, \& }$ ?0 Q; F 8881 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct7 Z8 }" I) L+ ~ n& O- [" v
8941 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg
3 {' O8 }* }: d2 h# Y1 P 9001 acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg gggcgtcggt7 E$ O$ e# P: g, M) T1 ~
9061 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg. D( e+ m0 S! i# L) O1 R E8 ?7 F
9121 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
1 v! e" L( i3 c& T 9181 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac$ F( m @8 U+ z0 B$ G" S
9241 caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga tgcctccgct! S. ^" S) J5 t$ H) \
9301 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
/ A* D# @. w! p" G& Z 9361 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc* S- c$ M8 }: o8 Y
9421 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt& I) k4 W" b* p; w3 u( `5 g
9481 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
9 \! C4 x" o/ p( I& M- |; F0 k* D5 R/ L 9541 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga/ P; l6 j4 G1 w
9601 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
$ e# u `) z1 \' a* M/ o$ Q 9661 tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag2 v2 H& z1 u l# H: G; w
9721 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc( R8 |5 N7 Y/ d0 _9 i0 Q
9781 aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc gggagcgcgg2 [ I: l, B: ^2 N9 r
9841 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta) B( d* [3 I$ J/ h5 b
9901 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
! l/ ], A; d+ c' J 9961 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag& P/ o6 a' o4 M0 O! I" g
10021 acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct gcgaaagctc
. [. H# Z. E2 w& E- k 10081 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat
) y/ f5 l9 Y" Z% n% i, e2 w4 Q 10141 gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg tcatccctta2 e0 p* r6 Q9 |* f
10201 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
2 c5 ~( j; R6 v" C2 ^7 N. [ 10261 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
3 n# R( i' @! F 10321 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
6 c8 A6 u: U4 o7 p! p4 x 10381 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
* j7 S# f8 o5 ?2 ~0 O6 }+ Y 10441 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
% w, L4 |2 m: H" [9 m 10501 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct
; V7 i3 S( J7 Y- l5 z" } 10561 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
9 J8 j/ o1 Q; h t' g x 10621 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
& U* E3 V( `6 k- W! r3 b 10681 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg8 q5 l1 K! G& S) g! k8 z- ^
10741 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc/ C7 `: ^9 v) `6 m- C5 U0 u5 d
10801 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca1 p# {0 O+ A- H! u
10861 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat6 B) S! Q- `6 Q. o9 s; w
10921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
# Z$ C q! X' a) x3 k8 ~: O4 o( V 10981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc) E+ v7 `' [: _4 r
11041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
- W0 P7 U* J7 K% k& m 11101 tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa
, ]: \* T. f; h* a$ E; J4 Q+ g 11161 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
4 L' z6 K7 C$ j7 f0 R2 [8 d' y 11221 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
3 \4 O& E% @9 H% i4 r L2 v 11281 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgagct# G. ^! i6 E/ q, r( S
11341 tggatcagat tgtcgtttcc cgccttcagt ttagcttcat ggagtcaaag attcaaatag. T: z$ D2 |9 G# V0 @4 w& R) u# l
11401 aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac$ L4 y* J& }" x
11461 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca
# h9 D( g2 Y" T* M7 X 11521 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
9 ?( {' u# d# y; d ^ 11581 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga* v8 j' q k1 W5 n* J
11641 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg. t7 X0 L6 U( P
11701 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
) Z4 k3 X0 q. d6 [ 11761 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
4 n n; H; I) a5 k 11821 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
6 y2 ?% q7 S. R c3 p' T$ V 11881 gaagttcatt tcatttggag agaacacggg ggactcttga c
/ K! \! n# {2 e5 X- ^//
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