pCAMBIA-1305.1
LOCUS AF354045 11846 bp DNA circular SYN 16-APR-2001
4 `3 i- h7 P) g5 M" P }8 |& kDEFINITION Binary vector pCAMBIA-1305.1, complete sequence. m( S$ r- [$ ?1 v2 q& K. G+ o
ACCESSION AF354045
- G/ D! ?; y8 ?% }VERSION AF354045.1 GI:135403462 j) M% Y! d7 r/ ~) l6 p" _
KEYWORDS .
7 J: ^9 R2 ?: n1 {7 s6 [SOURCE Binary vector pCAMBIA-1305.1% E3 r; z* j' o5 V" a8 r
ORGANISM Binary vector pCAMBIA-1305.1, N: \3 P; I- H+ H' A
other sequences; artificial sequences; vectors.
/ e/ C/ _4 j6 t3 V" P& |REFERENCE 1 (bases 1 to 11846)
7 C' S. e& l1 U* V3 x) D AUTHORS Jefferson,R.A., Kilian,A. and Keese,P.K.9 l" i2 j1 Q' D- c
TITLE Microbial genes for secreted beta-glucuronidases, gene products and$ k- a% ^7 i0 q3 v/ L! D4 ^% X
uses thereof
( U6 S- D7 G7 \* Y" J# C7 V9 p JOURNAL Patent: PCT (PCT/US98/19217)-A 09-SEP-1998;
, q2 R6 d4 ~. H8 Z4 Z! ] CAMBIA; Molecular Technologies; Canberra;
7 o8 @! a0 z2 ] Australia;. v. R- [1 [3 D& g* ^( e! N
REFERENCE 2 (bases 1 to 11846)% u* v; u/ B& `/ `
AUTHORS Nguyen,T.A. and Jefferson,R.A.. j& L9 _' v0 @8 C7 `! A
TITLE Binary vector pCAMBIA-1305.1( Q# R1 Y' f; Y( o, p
JOURNAL Unpublished
6 C+ W6 W K" z+ x. SREFERENCE 3 (bases 1 to 11846)
" z" F" o& p$ Q4 F AUTHORS Nguyen,T.A. and Jefferson,R.A.
) a9 b8 a: O) U TITLE Direct Submission
( w; \- k$ e6 S7 u; Q. }% d! ]6 s JOURNAL Submitted (13-FEB-2001) Molecular Technologies, CAMBIA, GPO Box: M" d% L, O+ `9 B" {
3200, Canberra, ACT 2601, Australia7 z7 @& M, u7 e) t3 s/ Y
FEATURES Location/Qualifiers& Y. }# C' ^1 }) m
source 1..11846
) n( V" O3 b& b" W7 d( Z/ j( | /organism="Binary vector pCAMBIA-1305.1"
6 N4 f- M, O7 V+ p! _ H+ v2 } /mol_type="genomic DNA"4 u; E3 e- h2 l+ T5 B+ _8 N7 I+ D
/db_xref="taxon:156490"4 u% P5 b( r) _0 {, F; Y8 }, k
CDS join(2..16,207..2054)- W( q& _9 M6 ]
/note="GUSPlus-His6"! H7 _/ G+ P( T( m
/codon_start=1
j# T1 W5 k. \0 R Y /transl_table=11
% j2 Y6 p( f( b, P# M; H) q /product="beta-glucuronidase"
3 D* q" u' S4 s/ K0 H' o+ T /protein_id="AAK29426.1"; S- Q( g# w Q9 B
/db_xref="GI:13540350"
) P. [, J( L5 x. Q' ^ /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYE& W7 V7 z) ~' w* H4 \
SKLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHK6 L) z% O. _5 t2 Z' c7 N0 D- L
AIVYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHE, d* S4 z4 u( h2 q4 n1 k
EGLGKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQ7 I! j( f5 Y T- s5 ]/ T" [5 n
GKAETVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTID
* K5 O' U, q9 x( {( {8 d; K: Z VYEEPFGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWI
4 y' s) \( U' S/ J: o( j U9 R3 s GANSFRTAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEK9 t) I, v. r D3 P/ [ b7 |
IRTFEHHQDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQK
% b4 {. X$ A& ?0 ?" Y3 F) e RPVTIVLFVMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRC
" O% `0 Z: ~8 @$ w# f# _ PGKPIMITEYGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFAD' H- P; Q$ L- z( i. s& Z7 F4 L
FATSQGVMRVQGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKNASHHHHHHV"
* d4 n& q; _/ R7 B- i5 s intron 17..206- `) a; D/ h* C, s! _0 \. l- {0 r# E
/note="catalase intron"
. e2 n1 i6 y$ {4 \) v. `; Z polyA_signal 2083..2335
, K+ {8 {5 y5 K% |5 ^8 d. ]- D G /note="poly(A) signal from nopaline synthase": _* g4 J* j0 T! Z% j! A
misc_feature 2354..2378. B8 }& l( P; H5 j/ Y7 m# Y% l; Y
/note="T-DNA right border"3 \, H' b4 p( J7 \; D/ b# t. v! S
misc_feature 3439..44397 z6 x' J; i) j. J6 e8 m
/note="sta region from pVS1 plasmid"+ W" Z& n/ q9 O; h
rep_origin 5032..60320 ?; ~( e3 p, M0 b9 U
/note="pVS1 rep"/ n. }$ c( o+ q$ @8 e, v
misc_feature 6442..6702$ t8 e( ?1 f. l" B+ @% b2 b; f
/note="pBR322 bom site"0 |: o0 m# g! k; _
rep_origin 6842..7122% b) E# a$ Y: y2 K9 s6 w* l! U/ A
/note="pBR322 ori"4 l7 U+ s( g/ ?, r0 L1 r
CDS complement(7413..8207)- ?* [+ d3 y. {* V5 w7 }. X
/note="kanamycin resistance gene"
; ?' q7 g6 v' [8 { /codon_start=1" c+ E$ G3 B7 h7 c$ t/ _, j
/transl_table=11
0 ~4 ~! o" `0 P5 x [1 G /product="neomycin phosphotransferase"
7 N2 Y0 }: [, X5 `( O /protein_id="AAK29424.1"8 P$ O+ B* M9 G/ w7 M1 {) p
/db_xref="GI:13540348"/ y6 V- d& D& \3 g
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK \0 e6 D' ^5 r3 `* z- ^
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY V) {' e$ o9 M" Y1 }( M6 A. F
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
" N* t2 ]; |- k, P( S: g+ A! K L EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
& h5 H3 _$ `' B" |0 T6 G YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"- u2 j4 P, y5 f5 c& ]7 X
misc_feature 8632..86571 j4 }) v6 |" R* C7 W0 t
/note="T-DNA left border"2 t/ V6 _9 N, E, a6 o8 I5 a
polyA_site 8798..8805$ P- x: B+ q: K7 d2 G
CDS complement(8948..9973)1 x. y+ X& l% G1 @% X9 v7 ]
/note="hygromycin resistance gene"! V7 N; |! Y: w" X @# z+ n6 M Q
/codon_start=1
$ P- }) H/ x G D y% k- c /transl_table=11
/ y+ }, a/ A" O) ]3 u) m /product="hygromycin phosphotransferase"3 P' j: ]6 P0 y$ T" `, s
/protein_id="AAK29425.1"
+ z; s, ^/ Y4 h# s5 c /db_xref="GI:13540349"
5 v6 c6 C* S( i) T/ B' L; o /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
; ^5 \$ R& y' t% J8 w/ J+ ~ GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
( Y: |1 C& @4 |5 A LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY$ j! g/ g" l4 I- S
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
' y- b4 g# M4 @# c EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ. [6 T( X2 e; c' W2 X [/ i8 P+ D
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
2 b. m$ u. \2 e4 d TRPRAKK"
' z. G/ X, ~/ H; ^/ y# e promoter complement(10009..10816)2 G* Z+ p0 N, S/ p2 d `
/note="CaMV35Sx2; CaMV35S eukaryotic promoter with% J4 O/ Z7 S0 c# R. A+ S) Q
duplicated enhancer region"
* X% v, O% e3 ]$ n promoter 10938..11018
. N7 |5 O5 c6 a5 o /note="PlacZ prokaryotic promoter"
; @( j3 X4 A2 b5 E( w1 n4 G! Z CDS 11019..11252- |+ ]- N+ }; Z, H0 E4 p& @/ F ]5 h
/note="beta-galactosidase alpha fragment"
) U' \4 H+ W Y% Y7 W /codon_start=1
* `, Q% D6 J! x% [ /transl_table=11
2 _. y: \1 L. {. } Q% t/ ^6 D /product="lacZ alpha fragment"
" f }1 q) V; K /protein_id="AAK29423.1"5 V5 m8 k3 n9 C1 m# b
/db_xref="GI:13540347"
7 ^- k( y/ z, E0 f3 Q Y /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
, n4 Q1 U# w& w2 i3 F' K' ^ RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" A% Q( v$ Z) W, K9 d& D
misc_feature 11033..11089' |8 Q! d% Y) z6 u" M
/note="pUC18 polylinker multiple cloning site"6 V( H8 U% c i, t6 L- J
promoter 11303..11840; A. d6 F' R- K2 p; w; P+ O# j
/note="CaMV35S eukaryotic promoter", k" h- ?% c0 q) a: |3 s$ H
ORIGIN 5 d; d: L. |% a% }# x c' e' J9 W- w
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
# E" S% O* f- K ^: P2 d S% B 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
" s7 i8 q9 B; m$ g* u% N9 @ 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
; J* R z; O: l r4 H( b5 q8 I 181 gtctatgatg atgatgatag ttacagaacc gacgaactag tctgtacccg atcaacaccg
/ y5 y) h6 P3 H! a* ? o5 }- T' X+ F 241 agacccgtgg cgtcttcgac ctcaatggcg tctggaactt caagctggac tacgggaaag3 ?! @8 y3 y, E/ U6 v# D
301 gactggaaga gaagtggtac gaaagcaagc tgaccgacac tattagtatg gccgtcccaa
/ N; b+ E* S' j8 M0 o 361 gcagttacaa tgacattggc gtgaccaagg aaatccgcaa ccatatcgga tatgtctggt; l4 r# ]& K" z- T* H
421 acgaacgtga gttcacggtg ccggcctatc tgaaggatca gcgtatcgtg ctccgcttcg$ D7 ^9 W, _0 K
481 gctctgcaac tcacaaagca attgtctatg tcaatggtga gctggtcgtg gagcacaagg
! m8 V# E% p: }* p% Q 541 gcggattcct gccattcgaa gcggaaatca acaactcgct gcgtgatggc atgaatcgcg9 w) ]3 s. x$ G E% m& ~# K
601 tcaccgtcgc cgtggacaac atcctcgacg atagcaccct cccggtgggg ctgtacagcg
9 g8 Q) L9 N( P( N* h4 l. Q 661 agcgccacga agagggcctc ggaaaagtca ttcgtaacaa gccgaacttc gacttcttca
4 u! `; M7 N, c, c; w* ?: V 721 actatgcagg cctgcaccgt ccggtgaaaa tctacacgac cccgtttacg tacgtcgagg
* z: ?! q+ k1 D, J 781 acatctcggt tgtgaccgac ttcaatggcc caaccgggac tgtgacctat acggtggact
9 d5 ^$ E! p& R- @/ G 841 ttcaaggcaa agccgagacc gtgaaagtgt cggtcgtgga tgaggaaggc aaagtggtcg
9 {( x7 U6 M- ~( Z$ N0 j 901 caagcaccga gggcctgagc ggtaacgtgg agattccgaa tgtcatcctc tgggaaccac$ y$ K4 _: W N2 b2 D
961 tgaacacgta tctctaccag atcaaagtgg aactggtgaa cgacggactg accatcgatg
+ {) i- T# r; l" x% f 1021 tctatgaaga gccgttcggc gtgcggaccg tggaagtcaa cgacggcaag ttcctcatca! R% B5 v0 ?/ _5 p9 e) `% q2 L3 I
1081 acaacaaacc gttctacttc aagggctttg gcaaacatga ggacactcct atcaacggcc. X; R: C; n. K- v/ Q- d
1141 gtggctttaa cgaagcgagc aatgtgatgg atttcaatat cctcaaatgg atcggcgcca4 t- Q- j, v1 m" R8 a4 @8 P/ c
1201 acagcttccg gaccgcacac tatccgtact ctgaagagtt gatgcgtctt gcggatcgcg( T0 b- n& J3 V% y$ i
1261 agggtctggt cgtgatcgac gagactccgg cagttggcgt gcacctcaac ttcatggcca
% A" G! [ G0 o' |, N 1321 ccacgggact cggcgaaggc agcgagcgcg tcagtacctg ggagaagatt cggacgtttg
) Z& B/ c9 k7 ?7 ?' K 1381 agcaccatca agacgttctc cgtgaactgg tgtctcgtga caagaaccat ccaagcgtcg
0 U* `7 e: d; X" |% r- M& ] 1441 tgatgtggag catcgccaac gaggcggcga ctgaggaaga gggcgcgtac gagtacttca
( S" |) Z2 h3 l3 @* f" i 1501 agccgttggt ggagctgacc aaggaactcg acccacagaa gcgtccggtc acgatcgtgc3 H8 P: ^/ U+ e# j, Y
1561 tgtttgtgat ggctaccccg gagacggaca aagtcgccga actgattgac gtcatcgcgc
* M. S) E( y) x5 S1 F1 D 1621 tcaatcgcta taacggatgg tacttcgatg gcggtgatct cgaagcggcc aaagtccatc+ l+ z2 u" \5 q
1681 tccgccagga atttcacgcg tggaacaagc gttgcccagg aaagccgatc atgatcactg1 H l3 H @+ Q: D
1741 agtacggcgc agacaccgtt gcgggctttc acgacattga tccagtgatg ttcaccgagg
8 g" T, h/ ]- \4 l. x- D/ N8 d 1801 aatatcaagt cgagtactac caggcgaacc acgtcgtgtt cgatgagttt gagaacttcg
. U1 ^! F- D8 ]: I3 Z3 Q) Y 1861 tgggtgagca agcgtggaac ttcgcggact tcgcgacctc tcagggcgtg atgcgcgtcc
6 Z: I* }1 @+ H 1921 aaggaaacaa gaagggcgtg ttcactcgtg accgcaagcc gaagctcgcc gcgcacgtct4 _5 I% B, D! q( F2 o: ?9 F
1981 ttcgcgagcg ctggaccaac attccagatt tcggctacaa gaacgctagc catcaccatc
+ F9 v, b% j& A3 m D 2041 accatcacgt gtgaattggt gaccagctcg aatttccccg atcgttcaaa catttggcaa
0 @1 @# R; x7 U7 d K" s 2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg6 F- M( x5 O- ?, f3 f
2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg( a5 I) g& \5 X8 v8 M! K" {2 [6 ]
2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag2 v# _. z; y3 y- m6 o' O% o% J" l' f
2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
% k9 T" M% k5 W8 D3 V 2341 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt" Z, Q y6 `) t D* q$ ^4 p
2401 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc6 k3 o8 w& b, {' _
2461 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
/ g3 c5 R J5 [( t! V" _ 2521 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa: o& U- B$ g% P
2581 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
9 m' L/ x( c9 @+ F5 P 2641 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
- `7 B$ H6 N# C. X 2701 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac1 _6 U( R1 n* m; }
2761 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac" H( u$ z$ \. k5 W6 t8 `6 H
2821 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
+ _# q) r5 C! W" }( U: _" D3 Z/ I 2881 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
% h7 S. r1 G6 c5 E4 y 2941 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
- D4 u; p' m7 A2 L; J 3001 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
7 A# V9 P9 v6 M: i4 @) V+ f4 _ 3061 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
. L7 u/ ?; K9 | 3121 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
% h- y9 J% q& q/ z) V9 D 3181 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
: q1 ]/ {3 z( v; o N* S" L 3241 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg0 R4 X& P+ }6 L% z9 t
3301 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
& y7 G5 Y4 u6 d- o 3361 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
1 x+ F/ j$ a. s6 D { 3421 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
' g3 y: q3 n$ Z8 ~ 3481 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
( m2 G0 {/ u5 g# U5 x 3541 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt/ Q1 @3 [0 @5 k9 n4 q$ ~" r
3601 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa- r0 ]% `2 V" s9 f4 ~, @, p1 ?
3661 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca3 X1 a$ f7 c2 B9 a
3721 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
% z% G" ~. O- n! H6 Z5 q 3781 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac- v) e9 F; P6 H! X7 I$ S2 P$ c
3841 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
' g/ w% t! f7 Z! `2 N8 [# ~1 x: G- l$ X 3901 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
; Q/ `! a- y9 {. Q" K, B& U 3961 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca2 ?+ D4 U# s5 O. N+ D( E ^
4021 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
" N5 k! i: M$ p! K6 j8 |& w) | 4081 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg. e* g* K" M& [- u( f
4141 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
/ C7 Y) F6 w- U: w 4201 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
I" p& c7 O5 d 4261 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
9 {9 a! g! I' {3 H$ Z 4321 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag* y% y" W9 g8 V3 b/ V1 a
4381 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
' t+ y7 T0 u9 u0 X6 ` 4441 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
' g/ `% J4 M L1 {/ y7 T; o4 ]3 o# ] 4501 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
3 Q& ~8 {/ W- i 4561 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca1 g+ d- V) v# H9 {3 I3 k
4621 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
0 [1 O; Z2 Q( Z 4681 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac, h1 V' g8 ~" |! n# _" S0 l
4741 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag8 \ T7 z u: E, v6 A, s
4801 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt) e9 _0 ~" t) p* G7 D1 Z
4861 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc, E( j1 A% O% o$ O
4921 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg% A3 W T$ t' M( m3 D/ I: a, }8 _
4981 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt5 B3 v% Q8 x: i" A1 H* p: d
5041 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
* G) ~; P& y G) y3 q! { 5101 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg; C5 j& B; L" y! t
5161 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag' d- R' p- z& V$ P9 E- @3 `
5221 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
5 c" c. l5 R+ A2 R% c+ t 5281 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt" |! B* z$ x8 q% I) G5 `" b- o
5341 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa& o* t( S, b- ]0 }5 }5 G6 R9 T5 q
5401 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
* q q# R/ u0 b/ |! A+ V {8 N 5461 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg3 j9 }7 j7 ]. |
5521 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg% y: X6 j% j- u/ P [
5581 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc/ W. e0 |$ ?+ l; \% ?8 m" E. u$ i1 u
5641 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
9 s& \5 F" m) a8 w" I 5701 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
4 O, ]; I5 M0 d% S9 E( d, \) Y+ W- O$ P 5761 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
* s {7 G6 ^; r4 j" y& ^ 5821 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
% h6 \. }1 n% ?5 e* k- E8 X; V4 g 5881 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac. I# c8 W1 I. [) |$ S
5941 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
: y" s; o+ c6 k" o 6001 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc2 o+ [2 G1 G4 J& P- H
6061 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
! u; V9 B& t Z W" ^! r6 ` 6121 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
+ r7 w* }1 |) j @ 6181 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
/ x; ^. ?9 n0 L7 n7 _, C4 V 6241 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
. u& a9 L& n% J5 T% e5 l 6301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg/ h" i: o- J% M" Q
6361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat$ g6 |5 V7 m7 m5 w' {, b" M. P
6421 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag3 V3 R8 M+ ^: h$ e ~; c6 p
6481 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa3 l5 n' n% O6 d, E: r) [! a
6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
( b3 z0 C% H+ o" } 6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg; F, e3 _+ Q5 ?$ o4 e K. X
6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
- I3 E% }5 A1 ?4 L2 P) b: Y 6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
: n7 v. A% p. `- I# A+ Y: J u 6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct- E* C2 w2 ?* `* I( v1 A1 h
6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
; J) ]1 d9 M$ b; `) }7 s @ 6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
$ p Q* k: Y: t 6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
/ p2 s4 y/ Z7 @ 7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca6 J: ^0 J9 e0 c
7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
2 Y1 G0 m) s0 W0 c) p 7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
/ L" k" o' b, \ l 7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
5 `* m6 e/ A, o4 u/ ?5 W: ]$ r 7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga9 P! s" x( `6 ^7 _0 a4 b+ c6 j
7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
! k2 K2 i1 z' E: b. D 7381 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa( D( O+ H4 S8 z* ]
7441 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
* p+ N7 ~/ J, z 7501 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
% ?% i! ]+ k2 u/ T& V0 W 7561 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag( S# F& w# _0 j" m0 t
7621 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc5 j# e. P' x) ]0 H3 s' }1 @
7681 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg$ m H/ Y; @+ e+ z
7741 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct! K2 J# G9 B5 c) ?
7801 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc3 c* P8 r% `8 t, Q- B' [
7861 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
4 c) G# N& ^6 d! [" S 7921 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg' V; x4 I; O( J0 h/ F
7981 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca( T5 n T- J, y8 ~9 n) y
8041 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
4 i' o, ^2 z1 r7 W# a' K7 x 8101 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat, D O. m3 |" N
8161 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
[# U: D T5 W0 D" B 8221 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca1 ~- A) L: G8 F5 `$ [8 F+ B9 k# W
8281 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt: ]- S" k3 ]: o, D- C& m9 m; }
8341 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac! O h. _: e& B3 J9 [
8401 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg8 @7 @" e) b' E. _5 t. G
8461 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
5 s% [4 n h6 d& P* |1 d 8521 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
$ K. [% Q3 n% K8 K( \* F3 K 8581 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat# ^9 W, N+ t( x0 n8 W# ?; `+ N
8641 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt0 U! Q" G7 P; g" D' K6 w
8701 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg4 p1 l% d. i: f0 A. ^0 F6 ]
8761 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
* m1 |( U$ ]5 O( \ 8821 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg. R$ ]+ H5 {# I1 R+ g
8881 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
# Z1 y9 O1 z# c# Y' Q5 p# @ 8941 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
8 ^8 V* t7 L* ]9 j% m( F1 _0 N% b: O 9001 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
$ J" x% i7 Q, o 9061 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat. t2 U' R. }- T; x6 d5 R
9121 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat- T S; F' S' w3 g+ L. ], J2 _
9181 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct E/ q! F+ D& H+ j
9241 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
2 g) P: n4 L# Q5 [ 9301 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca5 X$ X' E3 o) Q( a: \" @8 s
9361 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg! U& q& n4 F9 Z% X
9421 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
) W" ^( g" Z. ^' D 9481 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
. u1 [0 t, K' @: U 9541 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
4 L+ u8 S# I( `' N& a) z0 k 9601 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt7 j/ X* y4 S* c* X' m9 t, d0 }. N
9661 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
4 H) s6 T, S. Q$ w: _ 9721 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
% ?: X: @9 i& K" E8 Y1 L 9781 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
' @5 B: o" }" U2 q+ _9 O 9841 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca# ^7 L! W/ E7 g) A
9901 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
: [! I/ Q* ~1 R4 Z0 _' n {& J 9961 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
) h) l& Q8 D9 y 10021 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata) V! k3 M" M1 b$ Q6 c2 {. ?
10081 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat3 C& w; x) D/ A4 q. y) ~- V
10141 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc2 Z5 Q+ z* c2 `. K
10201 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct4 _1 ~- P- Y& p g! r6 i. s
10261 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga9 A2 h' [" }, \8 F0 K/ Y ^8 q1 N
10321 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt! h% d- v0 B( C2 u$ J& \
10381 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
* \1 o( |/ n) R, e$ S9 F+ E2 |2 b5 | 10441 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
" K2 q+ ^, G. J6 s 10501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt& w. _5 ]" M! d2 k) z# M! T) d
10561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
" } G7 x/ G y9 i 10621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
+ p& f0 B0 {4 g4 V( C 10681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
K3 D0 \! L! A; E3 @, f2 K7 g/ U 10741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
- z9 t% J* R5 S" L7 ? 10801 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
" i1 v8 w. F8 F v 10861 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta$ n! a' s5 Q: a( i
10921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
: r! ]( F u! C/ g' P 10981 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga
% e1 _# m3 o) z5 W1 u' r+ M% F. v 11041 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg
) W1 _9 ^$ |: `* G6 l: G. U 11101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag1 H. T- z9 {0 c$ d" }
11161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
9 l, _' a* z' u4 I6 r1 E. z 11221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg0 ? g! Y$ g& ~
11281 tttcccgcct tcagtttagc ttcatggagt caaagattca aatagaggac ctaacagaac
1 Z8 V X4 q- g8 { e! B 11341 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
0 k& U( U0 j/ r3 H 11401 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata4 q1 _8 u% `% h& n1 x
11461 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc/ a2 b+ `; E' q' X& x% o8 I# \ v
11521 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
7 i$ A$ _, |! Z+ ]( j0 W r" M* x Y 11581 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
" S% V: \' N2 N0 J6 I 11641 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
& m0 A7 T3 b+ c! A2 s4 `2 a/ X) j 11701 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg$ G; z/ p, v7 c# A+ y6 L
11761 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
4 ~. |9 | B- G9 Q 11821 tggagagaac acgggggact cttgac
3 D, [6 q; z* M; U3 b; l//
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