pCAMBIA-1304
LOCUS AF234300 12361 bp DNA circular SYN 24-APR-20000 X3 G' T+ ?$ g
DEFINITION Binary vector pCAMBIA-1304, complete sequence.3 S" F3 v4 z4 T$ ]* ^
ACCESSION AF2343007 u- z1 Y3 c' t1 g, o+ P
VERSION AF234300.1 GI:76380835 F j; ~$ Q5 T3 l; s8 e; P+ o3 w
KEYWORDS .
" D5 P" n0 f1 M# b( {, WSOURCE Binary vector pCAMBIA-1304- Y5 ^1 h" x. z
ORGANISM Binary vector pCAMBIA-1304
6 ^5 [0 c ^7 e5 b& k4 J2 L/ R! j6 T other sequences; artificial sequences; vectors.4 h; D; i& U0 d0 U5 A8 L
REFERENCE 1 (sites)
4 M9 |: R5 K/ I AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
. o3 k! Y. F& u* s; S+ B TITLE The small, versatile pPZP family of Agrobacterium binary vectors( J) G) r9 V) [) l& O
for plant transformation; G, G- T9 o7 z9 I2 K# Y3 k. G6 {- ]
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)( V5 e+ D' H/ Q* V* F& \
PUBMED 7919218
& T& U C8 k J2 xREFERENCE 2 (bases 1 to 12361)9 e6 ~# B$ r3 e, y! Y! X2 `! N( f
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
4 z( D, T k8 v Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
* S! B) V) H1 m: M Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
! ~$ Q% H# g. |# Z; G9 `9 b3 J& g6 N Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.( `! D6 w6 B$ E+ K; s: `
TITLE A comprehensive set of modular vectors for advanced manipulations% F+ S! `5 l6 b7 |9 H- {; l& Q% X
and efficient transformation of plants( T$ W5 x1 R Y& @, |- A
JOURNAL Unpublished
8 S- L7 E5 V0 M2 g6 q7 E REMARK Full description of constructs
, \( z! p, ]0 {0 U7 M9 B# P6 PREFERENCE 3 (bases 1 to 12361)
' [& l! |4 i) e3 m1 o! E8 } [ AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
* E! ?1 Y' }: q5 N0 V' D Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
|( Y/ x( m7 L2 Y1 U Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
0 E7 `+ b7 I' g% J8 m; C1 v Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.: x. K6 |9 O) h5 I0 D" g2 J; y
TITLE Direct Submission
' v2 w0 z! T! |9 a2 X: F JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /7 @8 `. J2 k0 V4 t4 {
GPO Box 3200, Canberra, ACT 2601, Australia
, A3 j1 E: O7 \FEATURES Location/Qualifiers
+ z& I) ` ~5 L# V) ~- M# b source 1..12361
# F+ L* z, t# x7 M# y+ @ /organism="Binary vector pCAMBIA-1304". `& J% z4 C4 L& B
/mol_type="other DNA"! C" [! R( v0 H! @' j) u; D! k3 p
/db_xref="taxon:118392"1 }7 g3 \" |6 ?8 P3 Q
CDS 2..2569; E7 [0 P' g1 n; L5 M7 W8 N% E- Q
/note="mgfp5*-gusA (N358Q) fusion (2-730/731-2569)"
7 I* J2 N' W) w% e: U6 L /codon_start=1$ S! X5 O$ a( ^& T; o3 `6 x
/product="mGFP5-GUSA fusion protein"
4 b/ ~. f$ `# H1 Q u /protein_id="AAF65352.1"
# V8 L$ F% l: D/ q t; g( n /db_xref="GI:7638085"
# o5 M% u' i) W3 | @. l /translation="MVDLTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG: ], E& u2 x8 p/ u8 c! ?
KLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI, m( W+ }" }! ]+ q# O2 ]
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
/ E5 T5 n4 i3 e- @ QKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK1 n6 a4 C: G1 }& ^) y& ?
RDHMVLLEFVTAAGITHGMDELYKASLRPVETPTREIKKLDGLWAFSLDRENCGIDQR
) h$ I( U, z6 @5 l" s, H0 B8 H9 d WWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAV
1 H4 P5 i, k* Q4 [' n THYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVI
9 S- f0 v8 K& |. O. d TDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVV( n0 P- l8 u% d8 x) ?* r; P p3 v# Z
ANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIY# C( G8 }/ z. x5 @8 C$ L. t2 c5 {
PLRVGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGA* G0 |* D0 _) c' l/ b5 I" @
NSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVN# ]1 V) g8 z/ t9 }3 c) Q
GETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTR7 L4 ~4 ^0 s: L8 o9 v
PITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLH' Z# q8 x- H7 U# t0 n
QPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFA
' a2 e9 i' L$ N9 v2 A8 P TSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
4 Y! T; s; N9 L% n6 O/ ~* c misc_feature complement(1802..1804)
* G" t8 r( S8 r( j& |( q /note="N358Q glycosylation mutation"8 t) q Y8 p0 x/ _* [
misc_feature 2598..2850
3 o7 r6 u$ ^8 v# g0 k /note="nos (nopaline synthase) 3'UTR (polyA signal)"3 Z9 S) R7 [- P% c' x9 W) ~
misc_feature 2888..2913
* w0 K0 ]; Y' L8 @: @ /note="right border T-DNA repeat"" h) Y* V* F% [% E% E ]7 U
misc_feature complement(3954..4954)
' J7 V, V* @1 x) R$ d /note="STA region from pVS1 plasmid"
1 H n- }1 W6 b* i" R) v$ | rep_origin complement(5547..6547)4 b: w( S2 d5 w: S, F
/note="pVS1-REP; replication origin from pVS1"
- z/ r& l; n+ [( e2 Z, a+ C; [. ~ misc_feature complement(6957..7217)
4 O5 e2 I4 e/ b& t3 Q+ b) a /note="bom site from pBR322"
6 B [1 \7 \9 V$ A" h4 \( G! _4 p rep_origin complement(7357..7637)9 N- u8 P) W# A3 {
/note="pBR322 origin of replication"& R' X( w; m9 J% U9 R9 ?' ?
CDS complement(7928..8722)) N& L. Y5 e' s7 o8 c$ ]6 E' n
/note="aadA (kanamycin resistance) gene amplified from
$ d' L' n: v8 n pIG121Hm"
F- [6 S5 s: P4 E& R /codon_start=1
# q6 g% s; T6 B: d( _+ o7 Y /product="aminoglycoside phosphotransferase": }- \& e0 X; v! o
/protein_id="AAF65353.1"' o4 T! {3 G l Y A" T3 m
/db_xref="GI:7638086"
9 P0 A8 q- \" R' X8 y /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
& [" I" W4 }' ~6 q MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY4 Y0 B5 |) G+ a7 ]+ K+ _& E
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
! O1 l1 L9 `: o9 K. e9 Z EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
2 {0 j$ c3 r3 w8 o& b YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
) [; e$ b' | N# q misc_feature 9147..9172
* H: D7 H' g! N" G /note="left border repeat from C58 T-DNA") Q$ Q$ N+ E! C( ^) M
misc_feature 9239..9447
, W8 h1 W5 i. v; N9 _% j, a$ i- \ /note="CaMV 3'UTR (polyA signal)"
( }* h! r5 A3 U% `8 Q) ?: {3 f CDS complement(9463..10488)7 ^$ W5 J# r+ o2 O1 @- R0 g7 s5 V9 ~
/note="hptII (hygromycin resistance) gene"
: F( o* ^8 E# t+ T- _" G( J+ \ /codon_start=1' V8 G: E2 M+ J9 s+ C# ]. q+ Q. r$ d, | {
/product="hygromycin phosphotransferase"0 `1 W3 e8 Z6 m9 o6 }, t
/protein_id="AAF65354.1"
" q9 T* \) v! ^8 |: e; d /db_xref="GI:7638087"- i- d( D1 C3 c. g4 N+ t
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
. ^- N' U" H9 x GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD: z/ X3 I* y7 v2 q4 H% ~
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY7 W" R8 D; w! L& H) E8 s3 C1 h i
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
$ d" I! e9 M6 I# J6 ~" h EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ/ l; X% D7 M+ Z/ }. B z4 [3 P. e
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
( n6 z. D3 n2 x, E TRPRAKK"
0 M3 q" I* G( H# f- G B* ~. X promoter complement(10524..11331)3 f; ?$ u- N. H; a
/note="CaMV35S2; CaMV 35S promoter, duplicated"6 k& o' M7 v$ f. a; a U+ `
promoter 11452..11506
/ d0 K4 O& |* @+ P. K7 ^4 a /note="PlacZ; lacZ promoter"
( ?+ D* m q" R: }1 ` CDS 11534..11767
' R6 @$ W# F8 W: |; f /codon_start=1
4 n$ a; T! ^5 o* B* [- A. `/ X$ G /product="LacZ alpha fragment"
5 ^/ W, U* d; B9 m /protein_id="AAF65351.1"
9 w/ e/ }; {5 a m( y /db_xref="GI:7638084"2 M/ ^5 B& [1 d4 {& e7 y1 A
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN+ Q$ y5 T% W4 ^1 @; J- a m5 Q
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"* M8 \0 I$ g+ @" C; M. h/ g& t
misc_feature 11576..11626; T0 u+ S! z6 {4 G o
/note="pUC18 MCS; polylinker"
0 h1 i6 q2 y& n: p promoter 11818..12355
& B- H D6 b m" y r /note="CaMV35S; 35S promoter from CaMV"0 b2 Y) E# M1 ^/ h
ORIGIN
* Z* ^& O1 F1 |! |3 s9 P6 Y 1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
8 [2 F1 v0 ?2 H2 {' h% T1 q# \6 g 61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga9 w" \6 B# c' _3 y4 `* H: B/ g
121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
8 ^6 t- n, @/ H; E1 I9 r 181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga: E7 Q: _7 u% ], D
241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
! J D' ^! `, L7 @6 Q, ^ 301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
5 g; V! _0 c3 E( n9 ^* P. D 361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat' A; ~: k6 `" [
421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
! |% R' b% B; d, K/ r 481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt- h* V- `! f6 l
541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
R8 Q+ o: i/ L7 ]1 m2 B% r! i8 X- e 601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga$ S! {- H) ^' |/ i3 ]# E
661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
4 W' X* v" Y% T 721 atacaaagct agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
( z% g" a% l" e+ |6 q& j 781 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag0 u& Y7 G. Z: U& u& ]4 @
841 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga: \* R4 H5 ? p+ W: C. `
901 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa3 G8 X d8 A; Q+ ~
961 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt# _ F) g2 H7 r$ m7 g! q8 R
1021 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga8 `# M9 j) T5 _5 e
1081 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
; _* {; s0 R! j- j9 l 1141 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa' y; h9 Q8 K [' C4 o0 X2 D8 A0 U
1201 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta8 q+ y; c8 k5 v, x
1261 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
6 \4 U+ a* d- K; x 1321 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
& [. M, N" O/ e' k, K 1381 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt& s: H4 J+ @# E
1441 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa, d* P/ a# u2 n) ^, \8 ~7 T
1501 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
; j8 F$ A# y n! ?8 }; E& _ 1561 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
1 K* ?/ _7 F- H5 i/ v J 1621 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt3 w8 p) a! k4 `$ X' A5 T% N
1681 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
j, A' w5 N) E' `2 G- k* M 1741 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
& ]2 r6 d _( W8 V: y 1801 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga" ?% w* ^+ n' ^ e' l* O& [: b) [6 h
1861 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
; w: @4 y1 ^6 X' r$ Z% J* ?. J 1921 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
6 |- d( Y% ]; z8 A 1981 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
b4 H2 S* n: |: c 2041 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag( q4 w- s2 M) [8 }3 B; m. I
2101 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
: n$ j4 c, w, |2 |* { 2161 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca* L7 z5 e) m. T
2221 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta) ]& `! ?6 | i: l0 [: i7 ?
2281 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
( n/ M1 Z/ s, f 2341 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc/ N/ J* d' F2 t) O
2401 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc1 H2 I4 ^( j6 ?
2461 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc+ e+ y/ N: q' h* u
2521 gcagcaggga ggcaaacaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca. J$ n! P" u' x8 l, Q
2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg* t! |8 w7 W" x d0 T' c. R% ~
2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa$ `6 Z& z/ l) j; a3 `
2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat0 _9 I+ p. x! f; M5 I5 P. ?# |
2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
4 v' ?6 Q& m; G5 G# U 2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata6 {( s( v5 L, S% R: H' A* P
2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
, U( M/ n$ K' w, o% N 2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg! R# s6 l; \8 H$ |4 `
3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
. h6 o3 h1 s" Q# ]- u9 \3 |4 q+ y 3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
# }; p& c, V% L1 g' y* e# ` 3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact/ Q( q0 ]* M, b
3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
9 `) p4 X3 N5 _! B7 L 3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
8 T8 ]+ j" S: P. b 3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag0 v) q" o5 i: |6 w0 Y
3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac: r; J! _0 r- J8 J3 F! k1 W
3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
, X1 P' ^- @5 f/ o2 q 3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
, T8 H3 Y( @+ v4 h$ s+ _ 3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
4 | L* L2 s5 r+ \/ b+ f 3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc- O& m; ` x6 V, g3 S8 R
3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
/ ~/ n5 x% L6 ?* j1 N, F 3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc2 a) c# R3 f. F3 U
3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
" D0 j, \% z; K% y. `4 @5 B 3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc. Z6 {7 [2 u2 m0 N M. n; m
3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg1 {/ Y$ _- P, g' A
3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
4 I) N. Z. ~* Z+ c% Z5 W 4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa' C# B+ @6 e1 x) h+ h v
4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
& f9 ^* H [+ F" k8 b( W7 r 4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
/ v/ G8 S- r) s2 W' G( g: q n 4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
+ a% g. p7 _( B4 a! P: v 4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
, {) ^/ a$ f- e/ y 4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
9 T! j" r/ |$ ` 4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
0 P% \' j9 y0 Q4 z' d* |3 m6 V9 P 4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
) f; Y" ?6 c% F# ~8 y, K) _ 4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa7 z2 E# B% V2 D! }1 v* I- R& y
4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
) D; h+ _) q+ i$ O1 _ 4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
, d8 N" O8 D2 ]9 m9 e- Q g9 c 4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
. K8 z/ a/ U' q6 V, q 4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
% J& l) b$ s. y1 g( N$ A3 |% U 4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
' C+ e' s+ G- I! ~4 v% V G) b 4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
5 @0 q' ~1 ?5 D8 F! o5 l4 e 4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
" k- [0 S5 z1 J2 T 4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
& M" |7 R0 Z' H+ Q! L! N' i 5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
. H$ g, d/ N$ D8 g |7 y 5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg8 ?- h0 t) n P7 Z- M* y
5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg$ e/ Q. O8 c m+ ~
5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
i) ^& I0 g- d# L- i 5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
; X0 b4 C( G& M% |! `- I 5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
: a0 D+ M* t2 l; G" g: ? 5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
# `* r; h: ~- }+ D6 K: D% l 5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
4 `+ i6 H: T' ] 5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
# \# I8 E; S$ Y/ O2 e: Z6 z' v 5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg: G5 r' a3 h( E
5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat" t8 m- O+ o+ {
5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg' P8 h4 X$ P% }& l# @8 s
5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg* y/ ~& ?( a: l# S( i3 [8 ?, K7 w' D
5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
, V e' K6 H& S7 B4 Q 5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
9 Y' \ ~, G' `' f8 r 5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
+ g+ b7 y( v* Q) |) B 6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
6 [3 y, V( t; t5 `8 \% B% {/ i/ y9 o! P 6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag6 f+ g2 ^: @ n1 o
6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga j' |, v4 [9 g1 ?
6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
7 `7 y5 s/ f! Q7 D* n! p 6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
) D( T( e' U6 b! A- p. A) G 6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
5 t$ F: _) R/ p( Q& { 6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc
- I2 @" C4 Z: b9 Q* I 6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt8 [; f* \# B& A8 l* m8 K c* A
6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
2 f. y. r6 w# r" m0 N; x 6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca9 `2 o4 g/ C9 p% {# n! P
6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg5 z* z3 Y5 h$ H
6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca+ Z) b+ ^- }- P! V
6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag0 C& B, U, J( Q4 q' B& m0 m
6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
2 z/ i$ ~5 W) y2 n8 @ {- ] @ 6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
" u0 \9 e* u6 }0 u9 F- W 6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
7 C0 M$ h; O ?) H3 }% D. m, o7 y- h 6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc( ~3 h% b. E0 q. K8 Z" l, x- T
7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt) y0 F6 H& A1 Z" @: S& w7 m6 N
7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
+ C' |1 e9 v& T. g8 x 7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag: t/ h4 g2 [- y& Z2 P
7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata1 H3 v; A! |6 W+ R
7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
6 l6 o, t4 R; G$ h 7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
/ q j1 F. X. o3 M 7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc* D2 X6 T J. L( B* r! r7 V
7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
6 P! n0 P5 D5 l; o2 b: {$ g 7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc9 s( b2 u, t1 c8 s( L* r7 j
7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga3 f! b; W, s/ ]/ C. w' n
7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
, h% V- B b, y5 R) m1 \ 7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3 k! w: ~$ w( d1 u- Q4 Z 7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg2 j* m: T( k2 o
7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt: [. H* e- L1 r9 D' ~
7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt6 j7 j2 P* f' A7 L, K7 A
7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct7 ^1 G+ S- F& w; [0 U& |
7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga, l& `- m ?4 M& o! Y4 K/ x
8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat1 Y6 D: `, ]2 I* x
8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
6 W; @# I- C7 b2 X8 {; P4 C5 J 8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
. L6 T; Y# `7 w2 {1 A 8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat8 V, W' E0 ?' w2 E0 @2 A |
8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
# C! B4 Y/ b1 ]& C 8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag8 N* ~% |& L4 m
8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
3 }. _8 S% U* M' A 8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
" p2 x, h% }3 \. d1 P3 X! j1 u 8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
* m+ w5 P; S" s5 K, u8 F! ? 8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
9 B2 `1 t" g0 r" n+ D2 ^" ] 8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
* [& Z, {8 P r. Q2 T+ F 8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
2 A7 p+ G7 B, M2 W' p3 z 8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
. T& ~9 n1 z6 t/ f 8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt2 i) E; B0 `. K. l$ J6 b4 W& O
8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
( ?, b1 w: @7 A4 ^6 P; f( M$ G 8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
$ H3 f6 b" L2 n1 H; ^! |$ g 9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
" I% w# Z! b& [) T) o 9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
# q& @2 L9 D0 | j: ?2 i/ \; R 9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg B5 w; L$ i @! ^9 X4 M* b. `
9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta% z0 ], i, m. Z* g, V* T( K: [8 p9 ]
9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga
B% i0 S2 b4 N5 f 9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
! u5 [0 C, H R# x B4 \2 j x 9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag
. y8 ~2 a" H0 b- u% b, e) U 9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg0 E& ?& B, x2 F1 h- W- Q
9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
5 o& R2 J9 X4 x- k 9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac, U- d% S$ b; \+ u$ _; R
9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
" U) i* ?% A7 }4 B u 9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
( b6 x" h2 _3 E$ H7 y3 j1 k 9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
1 P) ~6 K( N! @% f0 d& J& | 9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
* r2 p2 O0 Y4 |. p+ I" P" x 9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
0 z! [, I5 ]( V) r* Z 9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc: ^" |9 u# I0 \, [
9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg) G( q' _' o1 Q; W& r- b
10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
, g: b3 `. l8 E+ f 10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc( Q: }! W8 |3 Z# z' ]2 Y
10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc0 n+ t6 \ Q* B( b! h2 [
10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac5 F. f8 c/ l7 n- {" |7 [4 s
10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa6 b: e; t) q% b. K2 y0 F
10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
! w/ M) P# e1 c( R0 G 10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
8 J- R. e' ~3 }/ E8 t0 E6 M 10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
0 J1 o2 ^; t2 g" g m! w 10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc5 n$ N, Q# E) G! a( h' c, i
10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg
) _; K4 g6 `0 {& r9 I+ C; n 10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga! L4 n( p2 j. A9 E
10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg4 S: W/ A2 Z& l9 X* V9 O
10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt0 z, B2 x8 }8 Z. w" X$ |. j' R
10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
' Y) X5 t3 D7 N9 v 10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc( n" i1 t' k; ^; i4 }
10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
- M& [' n3 C! O \. c 10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct# A5 w% B) r: ~, s: j
11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc- m& T k! ]: }, y. i
11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt( q9 ^6 d- I) \' J9 u" i) i, ?0 w
11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg1 X- i6 M" Y; q3 e, P9 a$ P3 U
11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
' G9 g4 t6 Q* q" S* V p% D 11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct" x9 l+ V! x9 H& ]( ]
11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa7 i( n. G. y2 C
11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct9 d8 r+ I1 K" ?6 l
11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
; x& E/ Y# T$ M' i 11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
, w7 Q( R2 B5 v4 z3 e% W H( F- ^ 11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
+ F1 O: j: v: I# K 11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg- U- J0 |: p- o* W1 m3 G
11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga! \$ V$ E$ Q7 I9 F* W
11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat6 U1 n: h, n9 }9 x6 a( {# g
11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt8 J' W y- U* V( j, u3 S4 T5 i
11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
* p( y# s( X7 S3 \9 q4 M2 ?# a- d& j 11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
B# U' K8 |2 o" ?2 i; q 12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
* G# o- b) a+ a. Q* s/ | 12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
1 e: T# H5 Z+ G/ L 12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
J/ t! N) x/ Z Y6 N# [ 12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
+ h, ~! y2 x, X- Q* e 12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca/ M) Y. t3 s8 w
12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga
/ R3 _1 _% s8 Z* H) f8 y' L! @ 12361 c
% S* \! }! r0 q% k0 ? U5 y//
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