pCAMBIA-1303
LOCUS AF234299 12361 bp DNA circular SYN 24-APR-20003 m1 H* u0 H6 V% h1 _
DEFINITION Binary vector pCAMBIA-1303, complete sequence.* B2 r& N% \% i2 G
ACCESSION AF234299
% \0 `; J8 q5 J1 X& b7 m9 \ y0 `VERSION AF234299.1 GI:76380782 z% D4 h5 W7 w, z0 [; K+ Y4 o
KEYWORDS .
' _: L) u0 Z3 U, s- i" zSOURCE Binary vector pCAMBIA-1303
1 b3 H$ {- f2 y+ D$ m ORGANISM Binary vector pCAMBIA-1303
# @1 W7 p3 f* n/ w' E other sequences; artificial sequences; vectors.3 k# f: R: W! U; U- a3 F
REFERENCE 1 (sites)
q( Q9 c2 h9 U! @9 W/ I9 _ AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.& h% m9 d4 r8 N
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
2 i4 a/ n' A5 v6 w- v; P for plant transformation2 C6 k9 E! b, k# ?
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)! H+ }+ C9 p: e, o5 |7 [
PUBMED 79192187 x$ u# E. i7 X* f: c0 `
REFERENCE 2 (bases 1 to 12361)8 V/ ~6 ~: y$ n" v# E
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
& a7 ~# h9 L9 M ] Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
4 f9 h# m9 s2 U) W5 \$ { Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
0 M9 z* A/ ]/ n3 i8 h( ]) [& S# c; S3 I Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
9 |% g# F% d9 E8 }2 F. [5 I5 ] TITLE A comprehensive set of modular vectors for advanced manipulations7 L1 O0 v* ^- ]4 G4 o7 B8 W2 |( E
and efficient transformation of plants# y; B, {; |! m: j
JOURNAL Unpublished
9 s' I# d! t& O' J REMARK Full description of constructs
) ?$ c% r$ `. H0 pREFERENCE 3 (bases 1 to 12361)
: Q9 U0 ~8 m0 N4 C/ _ AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,; U7 J7 X6 {, f7 y% v! O, {# f
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,& i; j" Y' x( Q# Y/ Y! Q; h
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
3 M1 c% Z* t Z8 s: c, Q$ I Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.! v5 l- S- N- J& h* I
TITLE Direct Submission
" c& w/ o K. o; z& ?; a JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /2 s4 R8 S2 P- D! b* @
GPO Box 3200, Canberra, ACT 2601, Australia
/ b, [0 o; U5 ?7 r/ ?3 {) R( i& z: F- xFEATURES Location/Qualifiers2 o7 o* A% n, W/ [) g, ]
source 1..12361 p% U, `/ D0 r* C
/organism="Binary vector pCAMBIA-1303"( _6 y1 F; |" X! h7 K
/mol_type="other DNA"
0 [- P$ b' {8 } /db_xref="taxon:118391"0 i6 k) z j: f
CDS 2..25698 D$ | t" | f# w
/note="gusA (N358Q) - mgfp5* gene fusion; His6"- k' Q& q* b/ y2 m9 d" W( v& x- Q( d
/codon_start=1
3 m% k- \1 u( `2 i6 q6 O /product="GUSA (N358Q)/MGFP5 fusion, hexaHis tagged"
9 Y; p1 f6 l+ f$ k /protein_id="AAF65347.1"2 C6 r- t2 q' P A
/db_xref="GI:7638079"
1 G, q( i, r/ j2 m" b6 B) o( u /translation="MVDLTSLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESAL4 E6 \3 ] y4 ` d) ?
QESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKV
8 h; E9 P# `" h+ ~* y" V/ O4 e WVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK$ |& r% A3 W# O; w- ~
KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVS
5 j2 L/ P$ ]( j4 j. I VELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI9 ]; {. i% @& n" }( u; c7 k1 { }
RSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTS
r) B6 T2 g c. W9 [ HYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQA
+ e0 C9 f4 [) `( n! q3 ^# }- x HLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVN0 z7 M+ I$ T# V) X' V% S/ ^8 o
VMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIIT
3 n s$ M$ G& n3 a6 e6 U EYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGIL
% C3 F9 q& y+ Z$ e* n# l m% l RVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASKGEELFTGVVPILV
+ i- l& |8 q$ P5 E) v ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRY: X5 J% k1 Y3 j7 N/ r
PDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
+ b; a* @% X# X$ e, }/ W; [4 w DGNILGHKLEYNYNSHNVYIMADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGD
0 l) }, Z/ p6 [ GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKASHHHHHHV"
' Y7 ?" g( Q2 V5 w misc_feature 1088..1090
1 P d; J+ H* E" s, k, W L2 ? /note="N358Q glycosylation mutation", f& ?. [& {8 _( `8 L p$ K
misc_feature 2598..2850* e, L1 o! s! P7 K( e
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
( y3 r1 t' v8 K- M; t& } misc_feature 2888..2913; j8 J: V! ^) a1 _/ b* x/ R! T
/note="right border T-DNA repeat"
6 E# n6 Y# o0 Z8 A misc_feature complement(3954..4954)
2 O( ^. Z$ Q' y /note="STA region from pVS1 plasmid"
& M* ~0 d M; Q% E6 J, a; k rep_origin complement(5547..6547)
9 l! k5 x; N* J0 V, H, w /note="pVS1-REP; replication origin from pVS1"
' J& X' q+ r: D& d misc_feature complement(6957..7217)
7 L- P4 n) a9 t* l; u, J# n5 S /note="bom site from pBR322"
: P0 X X4 N: B4 n8 a% D, e/ Y' f rep_origin complement(7357..7637)
) _( I* c' Y3 d5 w: I. W, U /note="pBR322 origin of replication"9 Y6 c" s) c: n# E1 p+ ~) X$ k
CDS complement(7928..8722)) D; w8 q0 ?! u/ m' \) v
/note="aadA (kanamycin resistance) gene amplified from
# K+ i! Z. y$ a5 ^5 C9 }; {' L pIG121Hm"& |2 f& O% B$ B/ L
/codon_start=17 @% m% E& ?1 C* N: v
/product="aminoglycoside phosphotransferase"
* i% @; c/ c, y& x /protein_id="AAF65349.1"
O- Y% {6 U, E' j) H {/ U /db_xref="GI:7638081"
3 j" f* V. k& J; } /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
# o: y* j$ x( ^. d+ V- o MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
% v- x5 e' \( x, B8 q+ j EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE3 n- [6 I- i& [0 k0 g
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW( S$ W( ^4 a" C. F' ^2 K- O
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
& g/ F+ C- r) G8 t/ K& [2 Y misc_feature 9147..91720 X: \5 g" r4 r* e/ R% q
/note="left border repeat from C58 T-DNA"
3 k1 R8 b4 V1 J0 p/ F$ ?; Z) Q misc_feature 9239..9447
% j2 r# T; Z- f- i( z. N4 I/ E /note="CaMV 3'UTR (polyA signal)"/ H9 [; s" C$ |5 m% z( \
CDS complement(9463..10488)3 F/ t( g" Q- A) _
/note="hptII (hygromycin resistance) gene"$ E; E' }9 A D8 n% Y* {
/codon_start=1/ S% p) f% T; _
/product="hygromycin phosphotransferase"( R: b) Q9 t3 B4 Q* u
/protein_id="AAF65350.1": v" ~3 V# p& r8 L" e. H, C# f
/db_xref="GI:7638082"
$ u" o2 y% w9 X8 o8 S& u1 o1 e /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR) z9 O' K, k9 f+ z
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD8 h1 D- j# m, e! T
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY8 l- m5 A$ {/ z9 s
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
5 |" ~& `- r+ D) z' ?- | EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ5 ?; f7 ]) J, I5 q- {5 L
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
. ~! r: h m% ~8 F1 U( j4 ` TRPRAKK"
; X3 _$ n+ f2 N+ I5 y& M promoter complement(10524..11331)4 G+ J/ S) R1 m! I( n* a
/note="CaMV35S2; CaMV 35S promoter, duplicated"
% Z# ^& p: a7 ]+ u promoter 11452..11506
- M0 s6 v5 j# t m9 ^ /note="PlacZ; lacZ promoter"% U' N* p3 S- Y
CDS 11534..11767
, L! s I% Y. z) Y5 G: ` /codon_start=1
# s3 E: O, z( r5 L3 c" D0 ?& ] /product="LacZ alpha fragment"
9 v6 W _/ K9 N( C1 Y /protein_id="AAF65348.1"& K1 f9 |& C9 I6 I# z" h
/db_xref="GI:7638080"1 L+ I) }2 H! r H5 G7 S# L
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
2 g/ @7 ~1 U0 q; j/ C! P RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
% s) ]- R& x0 a- a( ?6 r* I misc_feature 11576..11626
& B- c( c ]6 X; P) M+ i8 H /note="pUC18 MCS; polylinker"' v$ @0 a6 v. V& K7 V
promoter 11818..12355
8 p4 R, z" t- n+ }2 I& L /note="CaMV35S; 35S promoter from CaMV"+ z3 V- D+ L, ^. m
ORIGIN 1 K# l# E+ z% }+ B( Z( E2 M$ Q
1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact! _* ?) l* x: @# H/ r
61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
* i$ y1 p& F9 c 121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt4 N! | x9 U) `8 P
181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
- E( G" G: U8 a/ L7 V 241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg/ a* f+ U0 l Z( H5 h; R: g7 x
301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga- \' ?; D$ Z4 _4 F+ [3 o0 x
361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
8 f: B6 k1 b0 [5 ]" h1 b 421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa* l) ^, O+ w) \4 t6 J
481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
' R( Y3 N( p" [4 V; }' A# a 541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca+ u9 S. a4 D; }
601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga2 }- w* s' c" [; E6 W' H
661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
" q& g& l; i- h' D2 s E 721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
, q4 ~2 H( H+ E& g _4 u4 Y" s/ C 781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
$ n7 m) o n3 ?4 h/ ?3 ]5 s 841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
7 w. b! j& d+ R$ }2 M7 X 901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca. ?6 D7 l9 |- S
961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga# l3 ^8 E5 c8 i$ [
1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt8 k z* N* {* w7 Q( x
1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
3 G7 |3 u" M7 c( M: q 1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct# r( z& X' @: J' k
1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
0 z; D$ j M0 O) n: v 1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact/ _, i ~! p+ t& ^- @0 D$ d
1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac: ], O- ~+ t# C3 z
1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
7 g: ]9 x) _! j! |* V7 }! C0 Z 1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa5 a( q; e. G2 A& A
1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
8 L0 x) _4 s# Z 1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg/ K: F& @) m0 X w
1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
& F. o8 c% r8 o: f4 w/ i9 ` 1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg7 ^- o5 {" p( P9 B
1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga& D4 Q% O4 J3 T+ `. b$ e* H
1801 aaaaccgcag cagggaggca aacaagctag taaaggagaa gaacttttca ctggagttgt, q# x6 h" M w: x3 p
1861 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga, k- ?* p; s$ ` m1 ~
1921 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa! L1 _7 r% Z" z6 u
1981 actacctgtt ccgtggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc
g' e. U8 m7 Q6 {( [. S0 A$ h 2041 aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata' g5 M- a9 ?! T& S, P3 ]5 ~5 O D
2101 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt0 ~! z, K: e1 i7 w; a
2161 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga
# I9 X3 z7 R9 x6 {: G( o5 t 2221 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat8 k- U% h" b' n+ d* q/ W
2281 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga
) m. j! W7 ]: z: X+ b1 A$ G 2341 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc
- b$ R, k& Y1 Z: i9 ~. ~0 V 2401 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa# o$ {8 Q6 R- U
2461 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg ~1 |, e( i: O% t- P4 n& G
2521 catggatgaa ctatacaaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca
' M6 G3 t9 j, X: z5 L& T: B 2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
9 N3 C+ G' S! s7 v8 i! Q: n) _4 f 2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa' m3 B8 W% o" z3 Q, B! A/ F- q
2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat$ V% f$ g" F) ], T. g3 K* D; C4 Y
2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc: p6 [6 y9 f& |9 m9 H
2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
% P6 C3 z' [5 D$ J 2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg" P, ^$ C0 j7 t) Y0 k5 O$ x; n
2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg3 S3 s' R4 O/ _5 e7 D
3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
: t% E" a/ v8 J2 r) I 3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc& O9 J$ h) m0 C! l/ _% G: H
3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
6 |$ {# W: C E: @' u, s5 ? 3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
: T6 q% a) ?5 ? 3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
7 o1 `* g- v8 t3 h% Y! }9 y& w 3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
( x1 h5 u9 v" y% e 3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac) G$ W# G" K- o* l0 h: Y6 p' R% K
3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc, N1 {; V. m/ C$ v. o
3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
9 a1 v7 L' O t 3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
- Y, P3 h! S, U J5 R$ T 3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
d+ I- n, W% }1 C# p0 K+ s; g5 } 3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa3 `, y& B3 ]; [4 Z5 I, o" _7 Y2 X8 L
3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc' w5 g2 g* o* d4 h8 V6 v
3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc( u" Y0 |& Q ~8 C3 P$ J# A
3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
1 Y9 M9 s" D/ W' q9 z' ?$ j 3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
2 I( v5 O, C( R 3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc+ |6 C, p* J% `7 Y+ y* P
4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
+ z) |: Z; R& d# o# B/ \& B7 E 4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg$ A) T2 F5 x7 U$ k5 s% ]
4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
9 p! |% J% k- \ 4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
# `) Z5 V- P/ S: j# G9 b 4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
- B+ ^, i# c! K! z4 F 4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
- A# z) s8 y' g6 E: P& n 4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg" g7 S9 U \+ `8 l
4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat4 J! u6 b" ]- |/ J; k. N
4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
: n+ @; T0 p' r+ [9 R: [9 B/ W 4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
+ V; M5 p( {5 \. ^ 4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat. A4 w/ }* `" G. q& }
4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac6 ^ j+ J+ j) T) \; V$ E
4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga% n9 x1 v; P, A4 k) u
4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
# s' F: k% e/ N- v0 m& g, V6 n 4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
, X! H* ?& g) U6 h! y. u 4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
F% L9 I. M% a# k2 P 4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
7 W( ~0 _; ~0 n; `, @) | 5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa. |0 c) `+ t S6 b0 E
5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg0 i6 d* e6 z3 s% q& |$ v- |& ^& g
5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg. H5 A. U2 c# Z3 ?$ d$ E
5221 cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac
% F3 H! b+ ?7 v4 a' |4 t 5281 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
i) Z+ j. {; |& `+ D9 d 5341 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc* Z6 v$ @7 ~" Z p9 Z. M
5401 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
7 g% w+ D: M5 T" O7 b* n 5461 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
2 w( }* Z }$ X& s. } 5521 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
# c2 d$ J7 m* Z 5581 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
9 Z* `7 U& }7 y* W+ ~8 j, L6 B 5641 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
E+ O) |9 I, u8 E9 S3 X 5701 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
2 ~1 Z& |! ]& k" q 5761 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
_( U, P: y- `" g& g$ U* F9 s: V: B. q 5821 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
0 ?9 u' d+ A$ k" W9 i* D 5881 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg( `' s5 Y/ Y/ ?) d# C5 \( I
5941 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
8 a" D& w h) b5 C& O C( l2 U* T 6001 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
* R3 R8 E6 E* C F( m8 I 6061 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag4 N" I# K' R9 q! q9 |3 m+ K0 o x
6121 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga. O7 [+ O/ o2 A' g6 @' J M
6181 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
* f- O' A/ a9 e* Q 6241 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
% O9 z+ k2 p" k: F- D/ Q- } o" j2 d 6301 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag8 h. [4 l8 e. }) x# A% x3 H, H
6361 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc% j$ A9 c: z! R2 ?( \) |
6421 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt% k% h+ B; y5 F" m; }3 M s* `1 Z
6481 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
. L: T* f8 ~4 g5 N$ a 6541 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca5 w( M) c T m. z/ ~
6601 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
+ S( e$ g1 s. O/ L/ w/ y) F 6661 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca4 g1 l5 I: Z" s2 l* G
6721 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag6 v9 Z' b+ Y2 ?/ O# h8 ]) ^
6781 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
2 a. d% a0 I1 Z& N7 f 6841 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
_6 w, k; N+ C- v5 E 6901 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
, D) S5 T; U/ Y' B 6961 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
f1 o% V! t* F2 J# R 7021 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt$ C& K( R/ l4 r/ M, c* J8 U, c
7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
0 w; \5 z2 m1 [7 d" l7 N: [ 7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
U" \3 D' m, y$ W- `9 c. d1 a5 F% y 7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
( {# U6 m1 R, }7 v2 O+ Z$ G 7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc; ?7 g) V: R5 k1 P& W
7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ]6 F$ O) @. Q$ n
7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
, C; q8 ]% Y5 c! m) w 7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
0 y! z' `0 o% {$ h 7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc, W$ G( N, X$ U* R: }( ^5 N$ |% ]5 l0 x
7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
8 x) w1 a6 T! D) |- h9 A8 b 7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
" w, ]8 ?0 V- f: P. R 7681 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa* Z$ V3 x1 u w1 E" M9 Q
7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
$ m+ ?8 k; W" E( H 7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt- y" u9 P2 O; v6 Y
7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt. R6 ?: f5 B0 t) \: m& q$ Y- p ]
7921 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
0 Z0 U( C0 q6 f( V) e% H4 _ 7981 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga! s) A; z2 C9 C3 Q2 w/ U% A+ p
8041 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
3 P; T3 }. N5 ]5 j3 z( P; n0 B# P 8101 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt0 N+ }) u Q" s7 b3 ~. r& X
8161 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
& x& ^! U4 M3 i2 @ 8221 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
# S( |4 E) F( d0 F$ f 8281 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
4 h" z% M, q9 ^# {( @* N7 b8 b 8341 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag: \8 E5 U- K7 A& Y; n- Y y% ]& Y7 L
8401 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
5 y! f0 g# V$ m6 k 8461 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
5 q V" f8 p- N, h 8521 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
: K* e( {% {& ?: R; U' d/ l 8581 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag4 m6 }: h) S4 q: o; |' ^, l$ g) R. n
8641 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg5 W4 ~$ ^) q/ N2 u8 {0 x( ]
8701 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat) c5 W- W9 m8 H8 P3 U# r
8761 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa; T. f g+ I5 i. w% `6 C v4 N: t& s
8821 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt$ W% g; \! \) U5 r# I( z. C/ }5 P
8881 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta6 g4 `+ T/ C. C6 J) ]8 o
8941 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc, E! d9 |4 l+ [) `# V) l; ^+ ~
9001 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
3 P6 a& m: Q5 v) ~+ m0 r 9061 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc4 O8 K# D- A, t) q2 o$ e
9121 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg6 X$ j: g- u- n2 L" s5 U/ P
9181 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta0 d0 N4 }" u1 h& P' n$ N" z
9241 attcggggga tctggatttt agtactggat tttggtttta ggaattagaa attttattga* ?8 A4 F3 ?% @ g
9301 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag8 F/ c4 _- a7 x% _' ~' P2 W
9361 cgaaacccta taggaaccct aattccctta tctgggaact actcacacat tattatggag8 Z: x; L0 ^ C4 G9 p/ ^% O
9421 aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg4 o$ U4 Z2 N! n( N6 i( p
9481 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca, o* @3 ?' T* w/ E
9541 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac1 o; y" Z; h! O4 m5 @
9601 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct' `6 |+ E) I7 N' D9 O/ e
9661 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc5 A7 M1 U# ~/ I; l9 P: ?! o
9721 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg# U1 a$ r" m Z
9781 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
1 ^' H) v; |) c; @9 L4 Y4 v 9841 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc; w" E* m! [0 o9 T1 h
9901 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
3 G I! ?1 {( i; N 9961 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg& v- c1 N9 e; r/ {" z% V
10021 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
# T" y' z) G. F7 D$ x: C* O 10081 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc' w/ j1 V$ v; P [( P" }# E7 d
10141 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
0 v3 ]! i k. r6 G$ I% T8 H 10201 ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac0 n$ V1 f( ]) Q( ] n
10261 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa+ V) F5 Z o3 {" i
10321 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa8 x# X$ J( x& u8 C
10381 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc0 H8 V' a, ^" o- y& j
10441 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc7 P) q/ m$ {4 z$ O
10501 cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
6 s# J3 N7 m9 _# P2 x; }% R 10561 gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg, _( `% k, b6 l0 E' i) A
10621 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga( U! o; v5 \5 I' D$ b
10681 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
- @& B7 {) c3 v 10741 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt! {) p2 A" Z I0 N; {' b
10801 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg) Q" h' P! q# f, s
10861 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
3 [; o2 w# P+ l; g6 ]! D 10921 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt# G3 b4 }1 a' r& c; } l
10981 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct/ c2 z2 \2 W* N5 G
11041 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc: t1 i8 M, { M2 l& U
11101 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
- h$ u3 \5 ]8 a" |! [, f. @ 11161 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
7 H( x4 k0 j2 c0 _- e3 r# N1 K 11221 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta+ _) `0 {- i2 C& V& L. g- C* R
11281 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct; }6 O8 b T5 O+ x$ H
11341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa) H- q; D# V1 W7 B# r+ J
11401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
$ P! U! @: d, F' Q. P* K 11461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
4 W8 L- k; A; a 11521 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
8 `8 F, L: S2 G; ?+ h" |% { 11581 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga0 c9 `7 R% m/ f8 j& \4 D
11641 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
0 N4 D0 u% ?; d% y6 ]1 ~ 11701 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga1 {" D m% p5 z4 M1 u
11761 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttagcttcat
, E7 P2 f. u2 _ 11821 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt$ n) N$ \- n7 b) o
11881 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
1 J9 Y7 m% O3 J/ o/ P: a 11941 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
E R$ E3 @! M6 m 12001 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
, u4 R& `7 n# \$ P 12061 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg- g/ x: j% R1 I* k1 V2 S
12121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc1 d, M y- P$ _
12181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt: c8 [: e2 [4 p
12241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca: x, a$ W0 b/ f7 f2 y
12301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcttga; j) h1 n7 j3 w: ]* W5 d
12361 c4 C6 x* D' L, a5 i9 x! D+ t
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