pCAMBIA-1302
LOCUS AF234298 10549 bp DNA circular SYN 24-APR-2000: E* J3 a" ~: z
DEFINITION Binary vector pCAMBIA-1302, complete sequence.
/ X" k7 w- v6 s/ Z+ @; oACCESSION AF2342981 g' x, n3 M: w$ p7 [ j/ I1 A3 c
VERSION AF234298.1 GI:76380735 M0 P. a. G' [2 E9 ?1 g4 _) t
KEYWORDS ." g) M( e- N5 c6 _
SOURCE Binary vector pCAMBIA-1302; f1 S4 q1 c* b
ORGANISM Binary vector pCAMBIA-1302( m& Q4 Y% G9 k( y
other sequences; artificial sequences; vectors./ f" e5 K- ?7 s+ r. v4 l7 O Y; n* A1 q
REFERENCE 1 (sites)
+ U! ^$ S7 k w, l; \) Q; A AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.( B, c0 U* N/ Q0 |) E
TITLE The small, versatile pPZP family of Agrobacterium binary vectors1 x A2 h% e3 d5 S/ F
for plant transformation/ Q# z" \7 d; w) T; z8 z
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
/ S: C! _) @# |0 w6 e, D PUBMED 7919218
# z% `4 O- }1 i1 JREFERENCE 2 (bases 1 to 10549)
9 G; s* K7 `. Q5 R! _3 `$ C/ C AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
; C/ N) H1 a- o Y) @ Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
# E, c6 a: Q& ~& `" d Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,) w- t3 G( s1 R/ Q: C
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
- @/ t+ i7 E( P+ x3 I- N) B TITLE A comprehensive set of modular vectors for advanced manipulations; r. M( U+ V4 L4 r0 O
and efficient transformation of plants
/ z9 C3 ]7 t, @$ C JOURNAL Unpublished
/ `1 ?0 j* d$ o ~# R7 u REMARK Full description of constructs
8 O# ]' z- n6 F& w5 u+ yREFERENCE 3 (bases 1 to 10549)
" [" m. ~+ I% f1 n( ? AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,! F: L9 d, [/ }
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,( M* p r6 i8 U. K& N! H$ T
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,- c! I' K i9 G% N x# T( n
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A." O% w" K$ p' c' ]' a! J
TITLE Direct Submission: M" d/ `4 {; T0 H! J3 F
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /, x" b0 a" n0 w% o
GPO Box 3200, Canberra, ACT 2601, Australia( F; a- k* x$ G% j, z* n6 p4 ?
FEATURES Location/Qualifiers4 p) j( }5 f6 ?; d" _1 L* U4 S1 d; J
source 1..105498 A+ s Q0 W" s+ n. T
/organism="Binary vector pCAMBIA-1302"8 E: m& |/ t. ?" ?! ]2 I& O
/mol_type="other DNA"4 G( u% m p' b
/db_xref="taxon:118390"- y; o# i7 j" H$ G6 Y) b
CDS 2..7579 u d v/ R# l! L4 O% p3 }
/note="mgfp5* His6"3 j( C& i- P8 E; I4 U2 q9 g
/codon_start=16 O% d) ~1 F: B7 ]" U
/product="mGFP5*, hexaHis tagged"8 z; ]2 B- }* H$ T
/protein_id="AAF65344.1"4 `' M8 t9 v! |4 i. g
/db_xref="GI:7638075"+ ]9 R0 B+ i2 b% @4 B
/translation="MVDLTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG7 j7 l- a7 `$ y# \/ R: ?: I! e* l
KLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI
2 W4 ~3 o4 ~ s FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
- T* l& d" _& l7 O1 H y QKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK2 b; e( Q$ L y( ]0 v
RDHMVLLEFVTAAGITHGMDELYKASHHHHHHV"
& F( k. {4 x0 i4 } S; l& r5 Y2 F# w misc_feature 786..10382 P3 B2 W0 E4 I
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
/ G) ? A! Z/ |6 F misc_feature 1076..1101
1 ?* \, X/ l1 H; t4 b& ^( D% l/ ?! A /note="right border T-DNA repeat"
/ Q3 V6 x; g$ g/ Y misc_feature complement(2142..3142)# g. t2 I8 }! P# _. ?
/note="STA region from pVS1 plasmid"
& V3 \5 r! ~' O rep_origin complement(3735..4735)
+ d* q* X8 H6 I( ` /note="pVS1-REP; replication origin from pVS1"
+ w6 h$ H1 E" U misc_feature complement(5145..5405)- ~3 Q% P8 m# x" Z- c* P' L9 V8 D2 }
/note="bom site from pBR322"& T- F/ N: u4 ?( B# k
rep_origin complement(5545..5825)
1 P2 Z- _0 t. o9 w1 }& z5 w /note="pBR322 origin of replication"
1 R# @6 c. w1 Q! [7 q0 y CDS complement(6116..6910)1 K7 Y/ M0 E/ K4 T
/note="aadA (kanamycin resistance) gene amplified from
0 D3 w. h; w( Y b" i3 f' ` x$ h pIG121Hm", A% C, ?/ l( z, t1 @$ @ q
/codon_start=1' |0 T1 j$ s) e3 y! n5 h
/product="aminoglycoside phosphotransferase"
+ b9 b2 U7 I2 C' B /protein_id="AAF65345.1": j! m6 c4 h- v* E! c# P8 e3 W
/db_xref="GI:7638076"
* t1 s; W5 S& c' q4 Q /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
# H* S9 x1 r( q0 ?' `4 s( p( D MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY+ K# U5 x0 D1 D; F- u! @) u' L; x v/ e
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
4 ^, P; m9 A6 v$ Q9 a; Y EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
; M. N% A; J$ X4 A4 ]4 }# [ YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"1 t7 _5 F1 Y- Y8 f* c; P" l& c
misc_feature 7335..7360' T1 t8 e, F& }4 m4 x9 V$ N
/note="left border repeat from C58 T-DNA"
* @: d3 a. F, V* q1 O misc_feature 7427..7635 w! B& c, O- u8 H
/note="CaMV 3'UTR (polyA signal)". O* x0 u3 d' Y J1 X1 p
CDS complement(7651..8676)
2 R9 z: i) A# x; ?8 Y6 Q O6 J /note="hptII (hygromycin resistance) gene"
" q7 ?( v# S, \1 K! `& K# _ /codon_start=1
9 z' [: N- X4 h' T: x' x$ A /product="hygromycin phosphotransferase"9 E: g6 l# f+ E7 B* m
/protein_id="AAF65346.1"
0 U3 b+ ]8 A* _6 I+ C" x/ U /db_xref="GI:7638077"
$ K$ _8 D9 [* Y$ _& G1 k2 U /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR" w2 d1 O) o7 p8 v% g6 Q
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
! T1 K8 U" Y+ S; ~" [) o LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
5 t4 U( `; V; a- [ [ HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS6 p0 @5 c0 M% |; t5 }) J
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ% o! a. g0 y U$ a8 T2 |
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
8 E4 p, _3 g1 @5 J) H6 u; I TRPRAKK". E7 {1 J8 Y+ g1 C, ~: c
promoter complement(8712..9492)' l! T& H, k ?* P
/note="CaMV35S2; CaMV 35S promoter, duplicated"' J4 g$ D* r' Q! j$ ^$ `8 n
promoter 9640..9694
. d ~, _+ C: }$ n' ]! S /note="PlacZ; lacZ promoter"/ J0 L& e, }0 _/ h, D; y/ U) Z; p
CDS 9722..9955% ?1 `, m0 u8 g& f5 n( o# q8 q) S( j
/codon_start=1+ r1 d3 W2 {! P8 d& b6 m2 \' ]
/product="LacZ alpha fragment"1 i" E2 V* _6 {4 [. J
/protein_id="AAF65343.1"
* Z( D% @- x6 x. u) h7 F& Q /db_xref="GI:7638074"1 ~- g7 [5 |& y, [ C, A
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN. r6 c4 P' N" ^8 L; @ A- Y
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
5 J% D8 Y, T2 k' Z% Y8 `$ l( D misc_feature 9736..97922 ]2 o% ^( g8 R5 Y8 T
/note="pUC18 MCS; polylinker"% w. m1 y' w4 d+ D! r! c8 r, I! n
promoter 10006..105436 D& E, |4 L* M1 p. E8 n) l' |1 V
/note="CaMV35S; 35S promoter from CaMV"
3 ^- C8 b6 W, g7 zORIGIN " [; q! [' E0 ]0 ^$ S' d
1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
# q8 w, B- U3 t 61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
5 _( Z) L' |- K7 ^& h. W) t3 | 121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc1 j5 y" M5 v( c, L: }( T, K
181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
% e3 n5 A8 z1 v; O 241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
/ n& x6 p- x' Q1 K7 Y7 E: p 301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg( _$ c1 ~" n! c8 w: z0 e
361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat; k* _4 l+ r& k6 w, i9 M; e
421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
7 l' m' K, \3 L+ N3 X% I# f$ M5 ? 481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt N( t5 Z9 q @6 `# o! _9 \
541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc9 Q/ g6 P b3 X7 ^5 V
601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga9 v+ `. B# }) V& u0 q: e$ J7 g
661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
, y1 }# x, r7 v) H. u 721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc0 `/ _8 D% R! q% s* o
781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg8 L* H2 v' s& ^; ? [
841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
/ N) L% m. i& m" X+ M" ?6 V 901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
) Q- f5 c2 o6 o 961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
3 M2 I$ u$ D7 G, |! i3 w/ m/ ] 1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
1 S, v) N) c: Y 1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta5 Z. S! {% ^+ F2 a1 s* I4 m% v
1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
g$ s" u' I+ R8 l 1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
5 [& T* }3 f" H8 Y; M( g, S 1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
' n1 o& v( Z$ G7 M" g 1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac! X: F( z2 z3 Z7 ~
1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc" y) E/ o4 T- ?4 _! Y
1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
9 J! R& b+ A5 T3 z" S! ]& x" ~2 L 1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
& p: f% J- R4 c 1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
1 ^: i, a3 l. t; c3 r" s" u 1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
& t7 m5 ]$ @+ O8 W 1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
6 O9 L* ?# c; g* M8 ?; ?0 F 1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
" w' O# E. f J5 y+ `6 e* Z 1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
7 i3 d' o ^ C7 h 1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
, w5 L3 c6 s; f8 N 1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
4 X3 M+ K& ]1 g/ a0 D 1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc% R, _. N- G+ V3 `0 J* k% s
2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
& Y I! \) d& R9 X 2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
Q- X% o# k' X& { 2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt$ @% |8 R) K$ X$ ?# Z
2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
* Q5 N" i* ]% m4 B" v 2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
: ?: s1 I! k; P% i4 O 2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
% X: N" m% f, ]4 y1 ?: l$ q 2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
- p: { X" b2 L7 O, J% w 2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
9 E* A: _5 o* l6 |- J L 2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga: l# v) |' t' J# Y# i: K
2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
4 v( a" F. ^! e8 ^, |/ ^" E, p. | 2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
9 B8 C" |& i% s: Z. l$ J3 ?- m 2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga8 A* }. A4 x& T# \' f7 l1 Q- g2 W
2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
7 q9 U- q9 [$ r! U4 q 2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg# E: S' c& m" g8 }% N1 z5 |; t
2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
9 L+ w; `4 y% B$ t1 ^ 2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
9 d$ t$ ]$ |8 B2 g% L 3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc; ^" E- B3 _8 h, t
3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca* ~& ^( y" A5 e7 D
3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac* I, Q+ X5 C7 {! z3 Y
3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
. F# ]) r6 i+ s" d) w 3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
# G3 h- z- G }0 t0 w/ ^ 3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
0 u- W9 S3 D( [) f 3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc' s; n% E& d& _9 k7 ]
3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
" f- n0 _2 i0 S5 Q3 f# I" j 3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
; I! {& W# s+ A, c; b6 _ 3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
0 I, w' l0 r7 |% F 3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
1 B9 [, H" a/ T2 e( ~+ ~; j$ Q 3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca0 w- z4 D$ x% `7 b
3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc Y! B# W' m* E) f1 |. H9 _
3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg# W% `# Q; R: k" N) K8 {+ G
3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
( }6 n; F( @5 z7 w3 D 3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac: J2 E+ m+ D" W3 U3 z- @- W
3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca' A6 P- T% |3 E x4 u2 i8 m
4021 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
1 f% f# F) d5 i+ V 4081 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa$ S' e, u* k, d K
4141 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag2 i- T5 f! p# F K* Q. w
4201 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca' A) @5 h/ h5 i
4261 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt0 ^" T! q7 A, S- L3 C4 x) m% A
4321 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
3 j, Q9 [8 d/ _$ O, W 4381 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg; u2 L! [: ^2 R% _1 w
4441 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
+ e" H* `# } C+ n0 |& j9 a 4501 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc D, M$ a. n6 k8 d# ~2 g
4561 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
. V1 W( Y- l3 O9 M! e6 g7 ?2 F0 N 4621 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
$ N# {% O; s* z! k& w& ]2 ]6 t 4681 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
6 M- \) Y+ X8 c. M$ `% e0 H, k4 \2 m- Y" C 4741 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc7 P3 q! m( A' F: _
4801 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
2 ^2 I0 O. C2 Q# f/ x2 P 4861 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
3 c0 p9 N; g4 O# z7 c3 ^& L 4921 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc, }' e1 c8 J O/ @1 `
4981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca* i; W" _+ Z' I
5041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt0 F# G8 L$ u; H, L7 D& V \
5101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ]* O, g) |6 X7 T3 B
5161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac8 U7 n9 B$ U0 w2 s
5221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
% G7 `/ H" x6 ?/ M: m 5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa# T+ t1 n+ `9 D! S) i. ~
5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc1 x$ r, s6 k: Q
5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
# S/ t! a# R- R; ` d5 E3 x& B 5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat- l* \( n( `2 m0 h, Q" z
5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
1 R* x3 G& s0 u, i, d1 f 5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
3 k M' ~& h$ h6 Y, S z6 N- A* \ 5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg$ f* P* Q$ T* M9 L4 y
5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc! V/ f# |- {. F* q% M0 c
5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
1 n* n/ r5 Q( E0 n( c) j, v7 N 5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
) k. c4 k4 u+ @$ L1 X, h 5881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta( v9 H: \5 G( ]0 r4 J
5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc3 K% @* Q0 f: e: E/ [- g
6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg" {: K+ A: Y# I& P |) ~ c, ]
6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
9 J: [4 t- c) W* n9 m 6121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
3 ~1 B" w5 N# a" V) Y 6181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
9 Z' Q" |, p# f& k, R 6241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac& t' t( S4 v4 u& L3 L
6301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
. v& d# G1 N e 6361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg0 x% A. d; [; t L7 `; X# J' R
6421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
( J" Y- u1 f# P" o' r0 [ 6481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg1 ^& ]1 h$ B, h# Z
6541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat7 o4 H6 r* `& O/ L1 ?9 e; y( ]
6601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
, E' }, y% t9 |6 b+ g 6661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
9 ]( p/ d3 C5 |) p0 n' W' F) [4 |* Q/ y 6721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt, b' s) y9 x3 c3 w8 {/ D
6781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt. g3 Y! `& ~. C5 d
6841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
1 r O+ i( j3 [6 S8 N 6901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc& z) Q& M( B# Q9 w" W& K& z6 ?
6961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
4 R$ n& r5 J0 Z 7021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc3 S( {/ T7 a" |# N$ M2 E4 e
7081 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc, t6 r/ Y: B z+ }% v' x0 o
7141 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat- ^! M) K! m1 ^9 }
7201 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
; K0 A! J2 t* ]2 K& V! t 7261 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
) W: Z( b9 G, \ 7321 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
/ y3 c2 k v, W' I4 h6 | 7381 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc7 n) N. G1 A# C) Z$ Q# V
7441 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt' F$ s/ X2 W* X8 T4 L) E6 l A
7501 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
4 h4 l$ |! t8 e% |% c. A 7561 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt$ k# M2 n+ Q4 g' k
7621 gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
7 `- A1 s7 m2 E C 7681 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
' V0 L: `) H% w: S 7741 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca* {5 J( B6 t. L1 r2 D7 O, n
7801 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg4 ?! d$ ]3 Q) g) U
7861 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
1 \, ], l: t. j# o; l$ ` 7921 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa, B% ]7 p2 C! R4 x
7981 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
) |6 Y; F2 r/ k8 s! V 8041 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
4 j, g2 \& t, k+ G 8101 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga! d0 p; n$ z& \
8161 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
3 h5 u1 y4 v6 }8 \$ f. O7 C 8221 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa3 O% @2 X# I& Z- K$ X* M
8281 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg$ s5 Z+ A: V% c2 G
8341 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg- d! u5 b [& B) m2 G) v
8401 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg9 A n6 ^* V9 j7 s5 h) M# i% ?
8461 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca3 z' ]9 _$ L! r% K+ f
8521 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc+ h( r8 k `5 h! T! v7 X
8581 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
3 y" H2 u4 \# ^) I% t7 t( x 8641 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc/ o: o6 d4 E" Z \; `6 p% h
8701 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
F% }$ ]6 z" M5 O 8761 aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc
' d8 |+ j+ M E 8821 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
/ a8 Q, Y" X) B8 f: z3 F5 e 8881 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc n( J6 u- @, D, A
8941 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
* W* d. {# }& o+ Z" }0 H) ?- I/ x 9001 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
}; K; y1 H6 _ 9061 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
8 X8 r- P3 P# q 9121 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc/ \$ B+ m: b$ i! ?1 M8 O8 g
9181 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
$ M% f$ h; A% s) {7 h& `- \ J 9241 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
% r1 S) f$ K& _ i0 ^ 9301 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca( p( U q! u' s! P
9361 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc7 D$ z! x$ S+ z/ n( b# g1 H
9421 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc& ~% I6 Q8 j- x, d5 K) g
9481 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt' x! ^& U2 C# K$ d
9541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag% _+ T4 y+ u& i% L4 R! ~2 E
9601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg& ^; U9 z$ b. A
9661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc- i" Z3 g" B7 P! a9 M) I+ z& t; y
9721 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
" M% n, r+ N* M) Y3 f0 o* {; F5 | 9781 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
- Q1 A- Z3 Y" L8 m" e 9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
3 j+ f8 c# q+ J: @ 9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
. N6 P2 x- @. o 9961 cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat. [( s( n+ |2 N* H$ @, U
10021 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct7 Q) Q( z3 G% H$ Q ~0 G
10081 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt; Z( H8 U8 m3 W3 v
10141 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
( d- i: K2 m& ?4 c9 J. Q2 s+ l 10201 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
$ b. c( m6 Y, F" I8 Z# L% m, a9 J 10261 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
# F- t7 x# I J6 \- w& u: _% R 10321 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
$ C" ]7 n8 N! b9 h+ F 10381 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga [. u+ f' w3 O; d1 H
10441 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
6 C/ S, A4 c" { F" G. t* c+ R6 O 10501 tatataagga agttcatttc atttggagag aacacggggg actcttgac
1 o* C6 m1 f6 o; Z% E) `//
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