pCAMBIA-1301
LOCUS AF234297 11849 bp DNA circular SYN 24-APR-2000
2 _$ r; ]' z6 H- p" O+ w- ~+ w4 {. E# vDEFINITION Binary vector pCAMBIA-1301, complete sequence.
$ H2 H, `& v0 mACCESSION AF234297
6 `9 \/ S' {3 q. r- jVERSION AF234297.1 GI:76380686 O. b% `4 \: h8 G# y
KEYWORDS .
: Z9 s1 n3 J1 y+ SSOURCE Binary vector pCAMBIA-13018 w) n5 J2 ?0 J% X
ORGANISM Binary vector pCAMBIA-1301
# J! \+ g, e/ Y; M other sequences; artificial sequences; vectors.
7 y9 z& Y( p$ h) DREFERENCE 1 (sites)2 w+ S9 O* o* D% T: w* M
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.' A* e/ X, `& A3 g" ]
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
. C2 T( [* d4 C! P for plant transformation
( g3 }; e( c& H( j t6 N JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
: `& w! W7 S4 l# D$ X8 q PUBMED 7919218
1 l2 t8 m9 T2 H) o& p4 |REFERENCE 2 (bases 1 to 11849)# t+ [, k; }4 B; a! T
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,7 P& d& v, t' V4 O( u% j: F; X8 x
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
& {' \/ [: `# T6 A5 u3 d9 x Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
% \+ v: D, T# t' {5 Y* B Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.: n% n) d \+ ^# n) c' g" _5 A. ^6 h
TITLE A comprehensive set of modular vectors for advanced manipulations% ]: c4 D. | ?' s
and efficient transformation of plants# q+ x' t& U! k$ _/ `% [
JOURNAL Unpublished) Y5 h0 S9 v# j, p2 {4 k( e, a
REMARK Full description of constructs: b* w, P* I+ j& x/ i9 I8 C
REFERENCE 3 (bases 1 to 11849): C# p0 ~% _! s# A: A3 x( |8 D
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
a* a; P2 Q. X% H Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,6 `' I, L" C7 o/ X% M# E
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,8 w" |" X" v* R8 o8 t
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
2 U1 E2 i5 v7 _9 Q TITLE Direct Submission
7 h8 y# @! K6 c p, L+ J5 d JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
; R+ A5 k7 i/ o/ l0 V2 v- i/ S! W GPO Box 3200, Canberra, ACT 2601, Australia
' w- w5 @3 Y" e1 ]" n% C* |; EFEATURES Location/Qualifiers
/ n# B5 @, R5 E% |3 ]. o source 1..118490 w7 L% p+ z, f8 H6 t
/organism="Binary vector pCAMBIA-1301"
Z+ j9 ]/ y7 X# S a+ q /mol_type="other DNA"
! o4 i8 I* K$ ?+ _1 Q /db_xref="taxon:118389") g( G. \6 w! S" p ?' O" u6 Y
CDS join(11844..11849,1..9,200..2047)% N8 |0 J; N' X
/codon_start=1
4 _( d& E Q, b+ e. r# M- e+ B /product="GUSA, hexaHis tagged"
( V+ M' h5 C9 |4 p' l /protein_id="AAF65342.1"7 x% ~. x; T5 g' [4 N
/db_xref="GI:7638072"# k" P5 E; T9 Q3 C; A
/translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE
8 i! r7 b& ?& K" ~: X SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY' I8 A4 Y" `! b d# X* u0 y
GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
" g% O0 g- A" S- J9 s- L NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG1 g3 A9 p; Y D& t/ \8 M/ k" a
DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR0 i- J# Y. X U4 ^
VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY4 Z6 S2 e& D( |$ ]. @
RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET$ b8 w/ T) l0 C. q0 r$ a9 [
QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT# A# S U( A& v7 K2 f" c
CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI/ l: D1 I4 M o+ e& E/ n7 {
IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ+ C0 z: F7 [1 J" W1 Z
GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"+ z/ Z! l# F8 f* N5 q& E) j- I, Q
exon 9..11844
9 h9 J: w9 @* _9 Z& Q /note="synthetic first gus exon (modular)"
- g! E, N6 B5 { intron 10..199
: I5 ]0 Q7 s0 {% a /note="Castorbean catalase intron; modified", t, @- X, q: l: U7 n
exon 200..2047/ \) R0 m" t0 A0 Z! ^4 c) u* [
/note="second gusA exon, His6") S9 A4 R4 e( g+ g7 }: `; ~, V
misc_feature 2079..2331
& C7 j( v0 k+ M/ i1 Y) T /note="nos (nopaline synthase) 3'UTR (polyA signal)"
1 Z5 I5 W) R' a0 R" e& z4 K misc_feature 2369..2394; N, c/ m9 r& j. B) p" |
/note="right border T-DNA repeat"
" V) H9 l6 w3 q" \( ?+ c misc_feature complement(3435..4435)
2 T+ e; k# ?* g /note="STA region from pVS1 plasmid"
' H! ~4 M# U+ f: G% ~1 C- |. i rep_origin complement(5028..6028)
! ~) M; J4 Y. w7 o /note="pVS1-REP; replication origin from pVS1"+ @- X2 i) E4 Q- d/ G
misc_feature complement(6438..6698)
" Y6 d1 }* G9 y- P. T /note="bom site from pBR322"
) Z8 j& i* ~8 u; {. q rep_origin complement(6838..7118)
w4 O& t8 E4 {' V /note="pBR322 origin of replication"
1 _6 v' L- c n* a3 S CDS complement(7409..8203)
1 y' w! |; `2 m7 Z9 f: M: | /note="aadA (kanamycin resistance) gene amplified from
# |; T! J. _( M' E1 I' N! ^ pIG121Hm"
9 F2 k5 ]$ `7 s, k" s& _( q /codon_start=1
0 T4 c/ W+ ?$ ^+ R m+ C# U /product="aminoglycoside phosphotransferase"
1 B% l, T5 e- l) I /protein_id="AAF65340.1"
& R) M( y8 H# D /db_xref="GI:7638070" t. M/ h# }7 g) }( a
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK/ x: k* M) \1 |
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY* N! k; A, a& H- n' k
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE: x1 C& \( N& f) O
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW/ b" w+ ?( Y% f4 o- B' q, _9 O
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
+ ?% }8 C. m9 J" p1 m2 u+ X misc_feature 8628..86534 \( C+ K0 X8 [. K
/note="left border repeat from C58 T-DNA") g8 h2 k d+ t$ L
misc_feature 8720..8928 U; `/ E; ]0 _ a9 X
/note="CaMV 3'UTR (polyA signal)"0 b( q, b- y# w$ n0 c h8 a# p
CDS complement(8944..9969): q3 O, ]* N8 y( i; z g* m
/note="hptII (hygromycin resistance) gene"
. I O& F" J% }5 H4 q /codon_start=1
* F& L: \$ d- N4 W8 H, ]8 ?- N /product="hygromycin phosphotransferase"5 n6 J5 r/ _! ~( d1 C
/protein_id="AAF65341.1"
; H, V4 ~+ ~0 ?4 m+ O% V' e /db_xref="GI:7638071"
( }: _" e: X! w, m. d /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR1 w7 T7 C+ \$ {" G
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
* a5 W% b9 v5 ^ ` LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
4 L, X. }8 `" B HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS6 d( A) {3 ]; I/ V
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
) V& s3 S T7 R1 Q SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS S7 e1 @( W- I" ~2 S1 S
TRPRAKK"
* B4 i9 E, [- c4 [5 m3 m: E" ~1 D* c promoter complement(10005..10785)" m! S y' c, |9 O
/note="CaMV35S2; CaMV 35S promoter, duplicated"
4 {7 o: L7 c" o7 A/ v* W promoter 10933..10987
: I0 [4 D* B; M# p- V2 b6 R/ A /note="PlacZ; lacZ promoter"% O G" N) G' L7 m
CDS 11015..11248
% a1 v. K# V( R' s4 A$ W8 ^* q5 B /codon_start=1
) F$ ]* T# R, M v( U0 | K9 @ /product="LacZ alpha fragment"
1 B1 J; K8 I! n( a /protein_id="AAF65339.1"" s4 v4 b- v$ G) Q& q' t6 p& F9 `' A
/db_xref="GI:7638069"" x( U& k) ]0 c4 ]' T0 B
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN- g3 Z6 i$ O1 }0 z$ X1 ?
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
% M' c& y0 B, o( b) S misc_feature 11029..11085% N! n- l/ [% n! {7 J; W! \
/note="pUC18 MCS; polylinker"
* N9 ?1 r3 _6 M+ w! r% t& V) g promoter 11299..11836
/ c Q" K$ ^1 ` /note="CaMV35S; 35S promoter from CaMV"
* `+ o3 h+ u9 f6 D+ Q3 {ORIGIN
i1 h: f& {" E, b4 D3 n 1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct; j. [/ n8 q/ [6 t- O' I% L! a3 M
61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
+ K- X3 a0 q2 e/ M 121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
% Y5 d O8 F* B0 e1 f0 ` 181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
# E/ e* \/ B6 s, b+ Y 241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
, p6 f/ y- y4 \; x& N8 Y 301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt% o) Q2 N; c. M2 T0 N8 L* N
361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg' V5 Q) G0 B' w+ ]4 }( i$ N& H, U4 c/ r
421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt; E% z6 o! l$ O; z1 G
481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
( a- ^3 C4 g* l0 g- g) O! m+ l 541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
2 y2 W2 h. }' [7 a& b/ ] 601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
' J. B. J4 c. P8 _; z 661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
4 w7 _) Z+ t, b1 l- G0 h7 [. N0 a; X 721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac3 k5 J2 U: N1 @4 v L5 N0 ~
781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
5 D; |- d" G0 e. J' T! j5 G7 \, m- ? 841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag6 c ~- a! B! a3 t
901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga. {- g) E4 p7 q$ B; @% K( p
961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
0 z/ F/ b9 |3 G7 p ]8 ] 1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac! t3 I Q. ~4 a1 z( y7 a" H+ I
1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
; q8 ?, Z& Z/ s I3 q8 t 1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
. u% C6 H ]! M/ A 1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga6 r1 i& L* i% i0 M* R$ K# R8 t
1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
( x: E9 ?. G+ g. m+ m) M7 j 1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
# D( G# e9 }8 l) n 1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
- |0 e4 Z0 J* i* |- K9 L! E& i; o 1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
5 V: l- Y |, c' i" H 1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc: c# Q% C, Z# u3 A; d
1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg' `! n1 C( ~3 e; O* n
1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
; _+ J; W5 G$ c) x 1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc8 D' m/ Z, n, ]5 j
1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga. C0 y! }7 M* d2 g1 ^4 O
1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt: i2 [5 `1 N' J( s8 w# ]7 m
1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
* u8 B o% ?0 L! H 1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat' Q2 ]+ y: S8 Z H4 C& C2 x
1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
* k3 p$ z3 I: R9 n+ F 2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
: b% i) Y" s7 U 2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
4 f7 ?5 p6 [+ t# \5 ^; o 2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt& d9 L# H- k3 P% ^
2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
% A# t; }7 K/ Y& j0 ? 2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
) }8 U+ C6 h( ~0 W2 U7 N1 N 2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa# V7 H# Q% E( b" Z' J; `
2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
Y$ c5 Y) U* Q 2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt: h3 h, l5 d$ R* y6 m" y( K
2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
- b( w# S. i" O# s3 G5 `3 j 2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
+ R% I0 `7 P8 a; R/ b8 F* I5 @. y 2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
: H, K3 s( n4 N% O 2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
5 u$ i) c- Q* U: ^5 X; u& p1 l$ s 2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc/ @! l2 F+ m* D
2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt5 H7 P& `& q% `' o A' |; q% ]
2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc* G' T' s' P" V
2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc6 f: s& h/ m5 @* W+ S! s
3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
4 ]" F0 n; i# H4 [$ T: n 3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag# I. \) E0 w! ?/ h) v$ H+ p
3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac! X+ W! R8 A, V* F+ f
3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg: m( j8 A9 p/ `2 _7 t' E
3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
7 I0 J5 y7 u3 ]! f0 I5 u 3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa4 F& i U' S6 ], I$ I3 F! `
3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag, z0 Y% W2 X: O4 r$ L
3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
_0 W2 Y$ |9 ?) \ 3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg( Y. f5 J! [+ G+ p0 z
3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt( S# R2 M3 t3 O4 _
3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
/ I; P w J; Q 3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
$ Q8 S2 w8 c/ U 3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt* K) A; l* H; P
3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
# M4 J9 K4 |+ d7 m 3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
) ~. U% c( k2 ~: Y/ D 3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
% |4 U' K) A' u0 B 3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc6 k: z! y1 Z& Z$ M" p- b+ z; |. Q
4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
6 u! R# ]; x5 U) F 4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
2 B. U; S1 d3 P! F8 e! a, ? 4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
3 L: u: V* p: P) e: s3 v/ r 4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
& T7 R* r7 B& x) ?9 p 4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
) } h5 c, l0 U: Z) Z/ g0 _ 4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
, y% G) [5 {( h+ G# Z 4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg' }" v+ O% ?1 B. w4 f
4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc6 Z* }, f" T% P: t4 m( v
4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
1 U g0 M' ?- I 4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga/ L% X# L. M4 K* x
4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
* e _" ~& j& j' Z6 M$ x& S 4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
1 Z. @" [8 g) E 4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt4 D+ y) ]' N; J% `- y6 P+ _( ^
4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
* m( H$ y+ j- {- J" h8 j$ v) i* G 4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
: V. ~5 v p" n 4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
1 |$ z+ s' L: I% J7 M( M 4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
" K5 G1 f( s$ q* T. X 5041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag& @. p* O$ U& I. ]! w2 E
5101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg1 g7 U/ }* W) k9 w
5161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
! g% P3 b. F6 N& ? 5221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg. K0 u9 t" {0 ^( x7 v# D( I5 R! @
5281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca6 N. O$ ~7 E' T( O1 C
5341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct8 G! `' b+ T5 {1 [
5401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
! `/ {) |% N1 h9 J: H2 o% u1 R 5461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
" z/ Q7 V, t5 ~& ~ 5521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac& ^# p5 a$ D/ z. {( [2 t: K. @
5581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
9 a \# p/ |0 ^( _+ W( N' Q4 n 5641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg0 K2 F" ], s) z+ J0 }: t. P9 f
5701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
+ C q$ {/ o( n& b% I8 j3 ~ 5761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc* ~3 X: f. u# S; G7 c$ G
5821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
6 ?4 H" ]0 C, K6 y# l1 L6 h( _4 I 5881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa7 g+ y: L" Z, B: P: h/ q# a
5941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
$ d7 z o- A* ^/ \4 \ 6001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg# w( u( [1 g/ g* w$ j( a) i2 K ^
6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
5 c: Y: A( |6 G9 i 6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa! ]; Z' R4 I5 _6 Q5 F) I
6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg0 y) h, ?! r z J" E& d
6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
3 c, O. c9 z1 v! n# E6 o3 r 6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
% K; r4 T o; Y f) ?% d/ w% D 6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc4 ~+ ~8 @: q0 Z7 T/ x; n( k
6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
4 J8 p# {2 d2 f7 R; e 6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
5 Q; D/ a( X, Y ?6 Z# Y2 A' S6 V, Y/ ~ 6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc8 L- X, A# ?+ s5 F4 ?
6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
8 i1 ~9 x4 d( p) c6 M% e+ f& ^! i 6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg* [7 H! Y% ` T j1 A+ U5 j5 e+ o) y
6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct% a: e, I! D8 y' V2 v/ ]* K
6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
( A2 s" C& i! N/ P5 Q8 N 6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc7 p9 R0 t5 K) e% ^* |% O8 h( t
6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
/ b2 v- J$ u( R7 @9 V$ z; T 6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg/ k! Z3 d' _2 j. G( ^7 j
7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg6 c5 r; }7 r0 [
7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct0 _* \, j( A0 ] v# H' E$ b% l
7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc+ Q4 R/ p6 ~0 W; Y5 x2 x, X
7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
) ? T/ E# K% x- x( J6 [ 7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc0 [2 X9 Y0 m! j: h7 {
7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt) h5 {4 R1 z6 T3 p& [$ T5 h
7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt- q5 u1 F) V8 A0 C; l, p
7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt( L, O- z, @1 l1 \
7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg0 K, u4 v/ k+ l8 L, g" p6 V
7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt7 T1 m& H$ r" }" i5 n# V
7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
+ {! F8 j) F3 B. n% [. C 7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat! X: g& R ?6 T, Q2 h
7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
- V! j7 R( @. X/ d4 z 7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
o5 S7 I3 ]5 E+ c. Q$ Y, s2 N 7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc; T4 g% _( Q! U4 i
7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct2 f) L& ^. N5 X3 t
7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
+ Q; ? k4 m" y: W- X. w 8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca% V6 z( V% U# }
8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
/ H7 a& D/ X2 s3 @2 h/ n& L) R+ k 8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc5 g2 b5 p2 R% z) e
8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
' Z8 Q6 G% f2 |* ? 8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc" r! m8 O5 v7 i0 X5 ?1 Y J5 k
8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc6 w8 |1 } L; P! Y' S6 Q/ d
8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
& A" e- k1 T* l& V7 n. z N 8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
9 w0 i" R' I6 ]- j 8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt3 ?( b+ c! ]4 [0 s9 ^* H
8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
- g$ `' r1 {5 }9 b4 M" G 8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
/ L k( _+ f, [4 [+ L 8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
3 s) x7 _ z! j' r8 X5 K 8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
) f: K& P$ u3 C: r 8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
. ]6 E) q% h5 \5 d, O1 H 8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct0 B' H/ s6 O' ?
8941 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac5 A0 s8 T4 o1 \/ P8 c8 d1 g
9001 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac5 {; O0 _/ V# Z# W' S# {* l( f
9061 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc1 ^# Y; {" `0 `# e/ I/ R1 k
9121 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
' [+ \6 B8 a; |" }1 T! L8 Y+ c 9181 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg4 }1 ?9 ]; e/ l6 a- X4 \% M4 ]
9241 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc; v1 T% z% G: G3 S, t4 [
9301 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
, b' V# r' ], r3 c/ S, K 9361 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
; q( J, F$ D/ G! j 9421 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
' u+ U6 u) W: V 9481 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
, a l. o: P8 e( | w, V 9541 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc2 G; z3 e* a# e$ [5 h8 l. u
9601 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg- Z: B& e2 C* E6 p& }( M
9661 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
( Q0 p3 f) U2 x! `: I# k 9721 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata+ _+ }" ]: h# d
9781 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
. {" F: B5 v) z 9841 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc/ y+ x8 ~- M" C4 |
9901 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
! G7 D% ]/ }1 Y 9961 ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat agatttgtag
$ Y E- R% |8 a# j8 }0 e 10021 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
4 t0 M' K9 k1 E' g$ l1 s) @ 10081 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca* y, D- Y/ A+ F- @7 O6 N/ m
10141 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt3 m( y, I+ u! [) V
10201 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
& g- V% k" T( @% w8 P+ } 10261 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa) g. x' E+ k" j% J% g% l3 h
10321 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa) R7 Z; D: X2 S& o {; P+ {2 D1 u) J
10381 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
; W& f/ p2 h7 ~; C/ I# t 10441 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac6 v- ^6 t, V9 q
10501 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc! I {5 m4 v' K6 e+ Q
10561 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
5 }5 J9 E9 ]4 P. F1 s1 M 10621 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag/ o( r) Y; j2 j% w7 L
10681 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
+ q0 J8 W$ _2 @% i1 a2 a 10741 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc" b; C7 C7 [" m" y; Q# y
10801 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac2 w+ `2 D6 {8 ?2 H j; r
10861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
% Q, r" G8 X/ h, R q% T2 E 10921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt8 a. [$ A# c1 D0 M0 F7 l2 p
10981 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgagctc
/ y- ~% }/ S5 {. X4 r( y: n8 S 11041 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt
( s8 `$ O% k/ W [/ F4 t 11101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca* ~; f# u) r: t
11161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca; ^/ G' n6 f$ W% p) f' V
11221 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
: [- D* M) Z y 11281 ccgccttcag tttagcttca tggagtcaaa gattcaaata gaggacctaa cagaactcgc
4 ?: i% P2 ~3 c 11341 cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat9 p* j! \. F3 N7 Q* t7 h/ r* @0 r }
11401 cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
& Q- e2 s& M- W& W% F! ~% ?& L 11461 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
3 x+ q5 o' h& `' |2 F* R, o 11521 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
0 Q6 ?6 i" W( a 11581 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga& ~+ v+ h4 O4 D" T4 w9 Y
11641 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc+ V2 ~, E! C* ~- s/ Y* o* w' u
11701 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
: {& Y5 ]/ Y% m3 A 11761 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
a! t5 s- B# k0 H, C; U7 h 11821 gagaacacgg gggactcttg accatggta; p4 D* p. G- y+ q/ Q9 e- Q
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