pCAMBIA-1201
LOCUS AF234293 12001 bp DNA circular SYN 24-APR-2000& N- r4 J: y/ x: J& ^ f
DEFINITION Binary vector pCAMBIA-1201, complete sequence.; J4 ^: b' [; A' H
ACCESSION AF234293
/ f+ r* x6 A% c XVERSION AF234293.1 GI:76380493 I& _7 C" J$ j
KEYWORDS .
& [' v% p. O) W, c- Z1 ?+ s: ZSOURCE Binary vector pCAMBIA-1201; d. b1 b; ^: r9 X: T0 Q
ORGANISM Binary vector pCAMBIA-1201
& Y# W) S/ n8 W. t' {/ w- B" Z other sequences; artificial sequences; vectors.
, J0 u/ b) h+ r z% H- `2 c9 mREFERENCE 1 (sites)
: i3 R1 a6 j" }- A! Y5 a& |2 G AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
. W- S& K$ q3 Y6 ]7 r TITLE The small, versatile pPZP family of Agrobacterium binary vectors
1 J/ c7 e- t9 p, {5 L1 h9 N for plant transformation) X7 C+ c( Y7 h% ~
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
; T2 w6 _% x* q/ N9 u) A7 E! H2 D PUBMED 7919218
* |& o0 V" h. x2 [- k! BREFERENCE 2 (bases 1 to 12001) N8 U' d# \# l& h7 Q8 J* [( k" B
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,3 Y) L$ H9 E" U: f9 u
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,# Q2 [9 S( T( z4 ~/ X
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
% W, A; P1 K9 y Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
% J8 U6 Q( W* I0 N, j TITLE A comprehensive set of modular vectors for advanced manipulations2 `& c( G! d+ A! O1 g
and efficient transformation of plants
4 C) p6 V' l9 x$ _. }6 W& n0 I JOURNAL Unpublished; s, T+ q1 ~6 K1 B+ L" i
REMARK Full description of constructs
" J& g2 N; D9 B0 I f& LREFERENCE 3 (bases 1 to 12001)
. @1 P W' k: t+ L2 l+ @4 h AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,, R8 J& p$ m- x9 a5 c5 [( Q
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
2 B) K5 H5 H6 L% t) n: ]- O Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
" o M( q+ ^7 G5 R Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.0 n# J3 h2 M; N8 p
TITLE Direct Submission7 Y+ M: h! e6 Z
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /& i% v. S0 Z# n
GPO Box 3200, Canberra, ACT 2601, Australia0 D0 k1 F+ Y2 ~" a
FEATURES Location/Qualifiers. t1 d& B! s" Z
source 1..12001: ]/ [/ F1 E+ R* g# t
/organism="Binary vector pCAMBIA-1201"5 y" L% n# w& k6 b' c# G$ q
/mol_type="other DNA"1 ^ a& u/ e3 g% P
/db_xref="taxon:118385"& z- H5 b1 x" ]9 s8 ?! b
misc_feature complement(981..1981)
) N5 O, M h; S! K0 I /note="STA region from pVS1 plasmid"7 }7 Y% `5 Z" r( N$ H. W4 v
rep_origin complement(2574..3574)
6 N) o3 |/ E# `) k- Y; l /note="pVS1-REP; replication origin from pVS1") D |( o) r2 \9 G
misc_feature complement(3984..4244)
% {$ Y9 \ M& P1 F6 \ /note="bom site from pBR322"
5 Y# L5 S5 ]1 c+ s8 x7 ` rep_origin complement(4384..4664)) Q6 Y# c8 ^6 q# h2 T# E3 @
/note="pBR322 origin of replication"
$ c( p8 K/ D6 p! G9 V% `! H) p CDS complement(5139..5798)
% q3 {8 G6 F+ R+ X2 C) O /note="cat (chloramphenicol resistance) gene from pBR325"" ^2 H2 G5 i7 n
/codon_start=18 ?& d) _9 B2 Q. e* E0 T, o
/product="chloramphenicol acetyl transferase"
. g* \: S; S( R/ g6 v/ c( u /protein_id="AAF65324.1"
; ]! s* ^5 |4 I U; @- P: ? /db_xref="GI:7638050"
9 ^$ a" \5 }# s' | /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF+ ]$ L6 B7 d% g6 _. \
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF1 D9 x: R; W2 c5 H2 ?0 |
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
- Q( d/ z, Y3 H$ L" Y' F9 F. v ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG2 \# h! \: U0 D3 w% p u2 S% D+ t0 H
A": ?: x' t. _ p! O
misc_feature 6326..6351
7 g3 G0 q( m% e* } g/ H /note="left border repeat from C58 T-DNA"
( x. z! g2 r1 w0 f% y; W misc_feature 6418..66260 X& N; s5 w1 \1 U+ w
/note="CaMV 3'UTR (polyA signal)"
2 v' x* @ f* z7 ^ CDS complement(6642..7667): ]& m, g2 n. M: q8 G8 @
/note="hptII (hygromycin resistance) gene", g$ Z4 o( R* L1 G# m2 J
/codon_start=1
4 M1 Q) D, f* Q /product="hygromycin phosphotransferase"
2 A9 u J+ {" o6 ^; Y. a: V8 J, [ /protein_id="AAF65326.1"# `+ C& Q" N0 |8 ^/ ?
/db_xref="GI:7638052"0 t S& e5 f0 G. [/ Z# O: m6 W1 l
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR. B- l/ l: j* u
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
. _1 b: I9 \" |6 ~ LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
0 R0 {9 a& Q. \1 p9 ?1 G HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
$ V. C1 N8 z# ^: [! { c EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
, g$ {8 j! O. {* U# t4 E" q& i SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
3 b7 }% q7 x: Z( w; Z TRPRAKK"
# Z$ g6 K+ E$ l+ s$ A. z* |6 v1 z promoter 7703..8510/ Q$ l& n+ z2 w/ L
/note="CaMV35S2; CaMV 35S promoter, duplicated"
: X$ q* o4 K. `+ ~6 S promoter 8631..8685& F9 W- C/ R# I* N' A: W+ D/ c
/note="PlacZ; lacZ promoter"! a+ o* x3 G+ i4 i& Q ~: ^
CDS 8713..8946
0 `0 ^" l# r0 H( ?8 r6 O /codon_start=1
# A% X7 ^" p+ ] /product="LacZ alpha fragment"
: I. N/ ~- W% k" f+ Y3 o; Q1 [ /protein_id="AAF65325.1"
& \. }6 I+ f! z W" h- [ /db_xref="GI:7638051"
. R9 B/ S4 V! U- _ /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN0 [& n/ y4 z2 J2 T9 K2 {
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
# v9 C( b5 K& E* ` misc_feature 8755..8805
& O* `7 a8 [6 O6 A5 [6 K K; w /note="pUC18 MCS; polylinker"
1 z4 l% L0 u& f9 i1 m& O: I promoter 8997..9534/ e4 |( H! v/ \, c
/note="CaMV35S; 35S promoter from CaMV"
6 x4 O& U6 N3 |) v CDS join(9542..9556,9747..11594)( `9 j) i- T( r
/codon_start=1
1 \$ l( E( D$ }6 ] /product="GUSA, hexaHis tagged"
% |# w" P. p, ]; M! F2 C /protein_id="AAF65327.1"5 E) q4 _. z$ I6 g8 g6 q, @/ b7 {
/db_xref="GI:7638053"9 M, u4 ?3 R2 N" a$ h' k
/translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE# h8 C: A6 R, j/ T, I& F
SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
! ]. e5 o& i( a& a7 O/ J GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
3 J# y/ B; Y/ g5 [2 s NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
7 }! ]' k# }' B4 E X DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR3 u- j! M" V U) b* o# U% X
VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY9 y2 d- f$ g5 U9 ^ k0 C* c4 F
RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
! z0 z9 o) ]; D5 y& Z8 b9 N& Q# \ QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT
* u1 T+ [/ J* z1 M* g CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
. i3 V4 j, P" L( }0 _ IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ
9 ^+ r) k0 P/ j4 E* B( u7 l2 I GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
- L5 ?/ v3 v, |7 Q; x/ G exon 9542..95565 T5 J$ n. ^/ m2 d' c. P7 O4 L
/note="synthetic first gus exon (modular)"
3 p# y k8 A0 e6 v. e2 i intron 9557..9746+ d* X5 c7 y$ b: k& a
/note="Castorbean catalase intron; modified"
. Q' Z$ ] w3 j exon 9747..11594) q# }0 j& F$ v/ T' P
/note="second gusA exon, His6"
/ V3 |/ n- O6 k misc_feature 11626..11878
: C B5 J# `) _2 O" g /note="nos (nopaline synthase) 3'UTR (polyA signal)" L' V3 N& Y! h5 V; X1 }
misc_feature 11916..11941- }& y0 _2 Q9 P
/note="right border T-DNA repeat"
' }- X: v' K+ j% k/ L7 {8 nORIGIN . q) l* D* k* x/ W0 l
1 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
9 ^% L% c3 d" f, X+ D 61 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac; V) R% a$ ?2 Z- d1 t/ T
121 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg! A% p* z$ p- H; w9 p
181 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
( D& O0 z8 P2 r' ]2 q8 [+ X7 F 241 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg5 [* a: j7 }) d+ _; b) d3 n* j
301 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
3 V5 }8 p% A, I3 \5 o X& ?2 |7 ?# s 361 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
9 W7 p/ ]+ I# `' N7 p 421 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac# \ s+ h# r: e# |
481 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc0 L; p( t ?/ q9 W. f, ~9 L
541 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
5 N7 Y8 n$ ]1 {2 t9 u3 U 601 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
* k, Z. j K8 l# v5 c. H4 L 661 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg! C N% s* z* O! y, A
721 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg/ T) \$ p6 X7 m3 I! Y
781 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
5 ~7 b$ H+ R1 d K 841 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
3 r F7 W: R5 v0 K* N 901 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta3 v$ j% d) j: g9 B
961 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
1 {' Q+ ^, Y8 ]( U5 [$ E( Z$ X# B 1021 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
) o8 P9 K8 q: F6 K: t$ i( L" V 1081 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat9 W2 G# \7 Z% H- J; R2 W' {6 B
1141 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
5 g# S3 R0 _8 ~: c 1201 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
# X: q/ ?, e7 G* W, L2 V4 I4 u 1261 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
6 ?, ^. A1 k2 l 1321 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
! W D/ v" N, ] f% w+ i 1381 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
3 n [' H8 `' R+ F( t 1441 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
( n. w9 k9 g* g! L0 h 1501 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
- b5 m7 W! t2 ]9 w" v 1561 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
2 S2 y$ f, s' m! M 1621 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga$ l6 M0 M7 X& w! |8 K
1681 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
2 d( W2 Y. ]1 i) E+ \( a 1741 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc+ s% z, ^' D6 V! @
1801 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt7 g% N2 Y, c) Y) g% U4 `3 ?# P
1861 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
" @1 o* N: ^8 O" V1 N 1921 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
+ g( w' p9 D, P0 \ 1981 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
+ |" s% `# K! P; K; d8 C* v8 t( T3 J 2041 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
: L9 ]6 h" v D; E5 n 2101 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
; U& {! N9 _- u6 w' I& V+ c: \ 2161 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg- i8 w& C! @- l
2221 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg. Y) k# R5 x$ p
2281 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
" x2 d7 ]+ F% W# [" y, {8 m 2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
0 j- P2 J. d4 i( X 2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
' N$ W4 @! g$ Q 2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
+ S; v, B$ V1 g/ g! y2 k* ] 2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
7 X, U% X9 ]. ~2 i) N& X$ v 2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc, R1 P: q! J0 c
2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
) B, I+ P! z; d8 T4 ^ 2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca/ B3 E) @& H9 d8 H5 b3 x
2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg# l$ |3 Y z6 A2 s- K6 B X
2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
4 |" ?9 T3 {4 G& n& X 2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
# `9 x$ ^ U# a5 n1 O- R M 2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
$ Z( @4 h9 m, k2 O% |& J/ }. l 3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc! H" l( T0 a( a* ~# w" U# `
3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc1 @- y& ~7 O) \9 ?, p) Q
3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
+ m6 K& _5 T3 s$ D0 c1 T6 X 3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
1 E" g. o6 Z. {4 u7 k8 } 3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag. l: \' \; }7 T
3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc" ?9 e6 o/ b6 E7 J9 W7 N
3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg6 ?, K, I+ A) [- Q6 F: i2 x% }
3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
4 q, b$ X" v; |6 ~1 n2 z$ G 3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa! `( x, T8 p+ O' t
3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
C2 a, z; g$ ?2 g# }1 l' d3 X( d 3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc/ o/ X8 ?% e a
3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
, E S) }9 k: H& C! h 3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc) e. j! S9 ^! @% {$ L
3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt3 Z8 `# |% g2 V/ z3 z* g
3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc; f4 k+ L! p" L6 ]% t5 Z T! q
3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc6 g, X" j1 ^% d6 Q0 e
3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc- z0 Y$ _- i! p2 W' r" k7 K
4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
; t0 f! e+ s# J" y6 | 4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc' r6 s; W. F4 J$ l5 x8 {! M" i4 q
4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag, V4 H. |& [0 z8 B: _1 u% o
4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa7 a" @2 `7 o1 B3 C
4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc7 [/ i- d( p9 o* _/ P9 ^" i
4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
! n4 z2 z! N% K3 m. j. ^ 4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg7 p, F' q) A9 R6 o- _
4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
1 T' Z; k, p' }: K) f 4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
- }, M; ?# L* }, W8 Y, a3 k 4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc+ E8 F5 W0 w2 {7 Y W" x0 ]1 E6 ]% {
4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag2 U! s% B' l: A
4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
9 x4 o/ m* M! h% z% G 4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa* N" u9 B) K; [* B: m7 S
4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag2 N0 e, G* U: k6 V9 v
4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
$ t! H! V( L, b( A( m: } 4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg
, g H9 S& i7 v: w7 I 4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg8 c) n4 a$ a) l0 l4 ], D5 b
5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt3 Y) Y" h/ U* N- r- s6 o6 X% }7 r
5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
3 M, @2 i; N4 {' M& |! y- Y: } A 5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
. u/ U0 D# a0 v( j U- z 5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc8 S" a3 q1 P4 b( o8 }
5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
3 b0 t$ ^# |6 j) X- P* u* G; Y 5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa. j L0 S. c% F
5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg7 Q% Z$ T0 z/ k* C+ p& O' O x! I
5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg8 D6 U0 ~7 g( D- Z) d, c! X9 m/ n
5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg' x% N L3 }8 Z0 M$ j
5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg5 i# {' I& @! @& }& \+ x
5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
8 }$ l0 @* [ Y2 p; ^0 z- M0 A) c 5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat9 }; U- K/ } ^5 W
5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg
" o t0 X, n& X8 }/ |! o$ o+ V 5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg$ L* o+ c( T* n# d
5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata# N; U: O- D" \5 ]
5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
: |6 `5 X& [0 S1 X 6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc
$ _2 k* N# v/ {4 u: n7 n. @2 C 6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
, ]0 L- N2 L, s% |& Y: `# B 6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
3 y1 ? m; i" U+ T8 ~7 i6 { 6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
* h) _% {8 w# h8 s% R 6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
, _6 B# C/ w$ w7 d 6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc( n. [! H: M: E; J }
6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa+ G! ?) V0 D/ m5 x& ?4 I2 O
6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat( ~7 J5 I" f. H6 r
6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc5 f* |) X; S% N* N8 a2 l
6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
4 X- a- i, S; y6 B6 i: v+ X2 c 6601 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
2 C/ Z- I! Y8 \% K 6661 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
/ n3 A! a! S9 z, u" J: Y& T' { 6721 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg6 w. y# q: b w" T
6781 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
1 D0 t B- L8 Y 6841 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca( A$ t0 F, o, b& B/ j' H i0 _
6901 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
) I i; R0 P' ^5 {9 @7 ~' s 6961 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag8 f* [9 @% I8 v5 y5 r* p
7021 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
3 x) e8 [; G$ W, ` 7081 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc* ?. b1 X8 C# D9 K; z& e9 h
7141 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
$ o8 s, S- | { v r 7201 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
% g% |! s" Z& n5 R# W: q( Y* G' p 7261 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
, R# G* g+ Y! P5 E% L( l 7321 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc- N7 g9 v: N/ P, Z
7381 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
- u3 X) P( M) {4 K' G* m- R6 ^+ i: y 7441 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
- j g. [' n6 g* a4 Q7 @ 7501 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
8 W6 C* K) o$ G/ L( q& A4 H; r 7561 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
* Z: y! P3 i3 g8 K 7621 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
( a2 ~3 s1 Z7 d0 I4 E9 u# D 7681 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg$ Y5 N X& x5 p+ U6 p2 b# I) p, \
7741 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg9 W5 ~+ Q& J' D( o
7801 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
2 S; G: w' J( q B8 m 7861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
/ {. ^- I& t( ?+ e7 i$ ^. N0 W, b 7921 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg2 q2 U, X7 w: m3 w0 B' m: N* D
7981 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
# |/ n0 j: Q0 X0 t 8041 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct/ }+ h5 M/ c, d0 b
8101 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta c+ w$ U5 y0 C2 h. p7 a
8161 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc9 B, V8 D$ j- s% {2 J/ d
8221 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
; F) u7 p5 h" r 8281 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg9 m6 s2 a& O" W/ O
8341 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt: E2 H7 b( w( o0 V
8401 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag3 R8 j1 o6 k' N1 s6 e: a4 P! [& y
8461 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc8 w5 A6 N5 I: ~" J" R
8521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
5 ?$ L+ y2 H. D; L( A! b3 ]& x4 D( D 8581 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt) N( Z7 w8 {5 O# H/ |3 d1 d# Y8 W
8641 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
% F% E2 K9 d- I; t6 L6 s 8701 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag" c/ p# n- y. i+ E
8761 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
% ?" q9 _& C% c+ P3 L$ L* m7 y 8821 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
P0 Q' Z& ]% A) F. o* j 8881 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
, r; `, g0 T5 q2 o) Q 8941 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt tagcttcatg
* u3 ^7 P+ E9 c7 U( r: N- x 9001 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
3 L: [* T8 h& }; U/ [ 9061 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac$ f3 q. [' b8 _* g0 f* W
9121 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
, Z! ~( x8 V& I: j4 { r- U 9181 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
, t% f7 O, x! W% u( u 9241 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc9 [2 \. q% V0 K; @) ~
9301 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
7 N0 A' j0 s4 `) j# c 9361 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg7 a2 U" V4 b6 @
9421 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa8 I7 [& ]2 Q7 U z: N
9481 gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg gactcttgac
2 T/ n p8 u" x' @9 q6 \ 9541 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct# \# P+ z9 p8 K* n/ `
9601 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
$ d+ A" g5 c4 S8 Y2 B" a$ L 9661 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
# N# r2 P4 S# y: f1 _# J: z 9721 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa i, M' Q/ J% h6 B& ]
9781 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
* |% h- D4 n4 Y6 s3 h% g0 p 9841 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
# A) v7 n2 M# z# }* V 9901 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt6 |) N9 R/ S- G" n. j
9961 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
t, d5 F% L. D& P5 m 10021 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
' x, |7 f+ Y }# F 10081 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
1 w( M3 _8 d/ G5 _( [" B9 m# O6 t6 E 10141 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
# Y! [& F6 W! X0 z8 K- d 10201 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg. M6 d+ |5 [$ Q! @' P* ]. b
10261 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
; o' Q% \9 j7 X0 U# {) b 10321 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca5 h( p- X# b' p& q! o0 m- J
10381 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
1 {) o, L0 p Q2 L5 j 10441 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
3 N6 W: j) @* z4 n3 X4 X& G 10501 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
9 t' w' `8 Q: k& m+ Y# h& G 10561 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
8 j: }1 e0 S! M% ~& U, w 10621 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
$ @" E/ o% C- u6 y, M 10681 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
: S2 t# P4 C# C' w 10741 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
2 a8 }* ]9 b7 K' u/ V3 z5 y4 o 10801 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1 e5 I' i0 t H2 _ 10861 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact3 ]) u' N" `5 o; V# y9 {- r
10921 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga( m' i |& V; W: v6 @) g( N
10981 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg# w3 @! g: \0 t' z: f
11041 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct2 B! b6 u! G' U, a. G0 T
11101 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
{( a0 [" k6 @+ j- } `8 \ 11161 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac. x0 y4 j, }& T0 Y( c' C" h
11221 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
! h! B& B( ^1 r3 q; z 11281 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
( i3 C; v# t' Y. [8 d9 T 11341 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat# \8 _& C0 c* _
11401 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag" Q( Y4 M" T4 ?
11461 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga0 G1 J, Q6 C) }) k3 r; E
11521 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc- g: e- |$ V) b: T! b4 _3 G, m' U/ X, S
11581 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
7 |( H( J) c. A y9 ~% n' L) Y6 Q 11641 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
. z; G! B! x/ G$ T+ q" a 11701 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
% F( d0 \8 a+ r 11761 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata! @* B( j+ V) x$ t& g/ d/ ^- Q
11821 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa7 u6 x7 ?6 S* C5 e: J0 u7 R
11881 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt4 t' N1 A! Y0 Y: s
11941 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
( f0 a( _ a/ l' d" T0 { 12001 t+ ^ `/ u% M& j+ O! Q% J7 R0 Y
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