[质粒图谱] pCAMBIA-1200

LOCUS       AF234292                9110 bp    DNA     circular SYN 24-APR-2000
! v) p) ~& B$ \8 e% w0 e4 z8 ZDEFINITION  Binary vector pCAMBIA-1200, complete sequence.
8 G8 [6 M! O. g/ K: w2 f+ uACCESSION   AF234292: |4 R; B6 R5 v2 i# j
VERSION     AF234292.1  GI:76380453 a) u1 \5 y+ t; c8 n
KEYWORDS    .
% [# I- d, R* A( ESOURCE      Binary vector pCAMBIA-1200
! p+ M+ `; o& f* i! G  ORGANISM  Binary vector pCAMBIA-1200
( A2 @# h! `7 p# ~            other sequences; artificial sequences; vectors.( l! E$ w2 ]/ C1 n5 |: g* S( S4 \
REFERENCE   1  (sites)
) K5 G- |5 E# ?& q! ]* ~  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.( z: k# n, _& O* R1 I. _" }1 \
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
# ?. u; C: n! w* e) r( D            for plant transformation% N# h9 r1 f2 r+ c) D) i9 U
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
. v5 P. T" J! e# p   PUBMED   79192185 z( o) K7 [/ {( C- n7 K" U$ a( U
REFERENCE   2  (bases 1 to 9110)
- c, w! m* n7 P2 A( l2 j# d) }$ s/ a  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,; L, @2 j2 Y# `6 [$ l, a
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,& \  h& p- ^5 W- h' f$ j
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,. F7 l( j# {6 Z
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  |2 F6 V& P% Q3 b  TITLE     A comprehensive set of modular vectors for advanced manipulations/ D  ^, |) j3 j, |6 F4 f
            and efficient transformation of plants
6 u. [- Y/ {& H  P6 L  JOURNAL   Unpublished
' ?  }: S& Q$ i) `  REMARK    Full description of constructs
# X* T5 w; t9 k/ e( }) hREFERENCE   3  (bases 1 to 9110)
! n' a/ |( Z* Y& ~! b. h% ~8 z  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,8 x, w; v% z$ h, J' P8 x" Z5 l
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,1 u3 p# L" [6 X$ a, \! g. X+ ^, E/ i: P
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,1 H  a, ^% S6 J! j! I+ X
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
5 k4 i9 b3 u9 Y1 l  TITLE     Direct Submission
7 g3 c* J% a) m0 }1 r% r2 v  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /& e( q& H* g  Y" |  s
            GPO Box 3200, Canberra, ACT 2601, Australia5 Q+ F* f; Z* G' X" @' W9 V1 M% z3 e3 x
FEATURES             Location/Qualifiers3 ]% |$ k; ~7 c% u# b% x, m
     source          1..9110
0 Q! _9 d" s  T3 Y$ _7 E; J                     /organism="Binary vector pCAMBIA-1200"
# e& N9 j) d5 o9 y& V                     /mol_type="other DNA"9 G2 L4 q( P7 `4 `$ R0 A
                     /db_xref="taxon:118384"
3 z  i6 |# }, r  Q" M7 Y& ?     misc_feature    complement(981..1981)
" [% E2 \, [: a2 c                     /note="STA region from pVS1 plasmid"
! i1 t; B1 z8 F     rep_origin      complement(2574..3574)2 H* t% T# t7 t+ U" X' V9 @
                     /note="pVS1-REP; replication origin from pVS1"" r# j$ |3 _6 l! w
     misc_feature    complement(3984..4244)
6 Z, s& y8 A' C$ f) i7 R                     /note="bom site from pBR322"
6 u7 B2 N: Z3 `- Z: p4 y. V     rep_origin      complement(4384..4664)
. I8 E" M1 ?. O( V7 O2 r                     /note="pBR322 origin of replication"
# s9 q- M1 M4 g& o6 e1 V! h     CDS             complement(5139..5798)
8 C' }' w7 W+ u* w/ X5 x                     /note="cat (chloramphenicol resistance) gene from pBR325". c, ~. b4 \  ]) L" {, n6 d& D; l
                     /codon_start=1! i6 {/ C' t$ I) G) |5 [5 q
                     /product="chloramphenicol acetyl transferase"
% v5 d6 j8 L! _( d5 J                     /protein_id="AAF65321.1"2 E* N) Q+ i- d  o5 P
                     /db_xref="GI:7638046"9 |+ x) i) A5 V" B- M* c: G
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF& E( a% L; @* i9 z' g  M" h! m% i
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF/ ^: O6 o* Z2 i# N. _3 y1 c9 b
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
4 U& j  F* E$ ^                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG, L4 v4 ~% K/ t* F3 M
                     A"( l0 H: v# n; q7 z+ @1 a
     misc_feature    6326..6351
* w- x/ B$ X: q7 B                     /note="left border repeat from C58 T-DNA"
+ L. y& H! s: o) L" R* G- a7 i     misc_feature    6418..6626  U& E6 u7 h' f% `# I( u
                     /note="CaMV 3'UTR (polyA signal)"
; N2 Z$ _4 C7 F2 \* q- z! m/ i     CDS             complement(6642..7667), p* W% w! y. Z! a2 E6 D2 q6 |  v
                     /note="hptII (hygromycin resistance) gene"
; [3 P! t& M2 J! k1 u5 C                     /codon_start=1
- m, B( ]! o& |# ~% {2 s                     /product="hygromycin phosphotransferase"
1 Q: o/ @; {. R0 y, Y4 e7 d% R2 K                     /protein_id="AAF65323.1"$ e+ O7 E+ X) `9 j9 h4 I
                     /db_xref="GI:7638048"- i& Q. B9 m9 w# v
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
$ Y" j: B6 Z1 L                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
, K0 U! r1 S0 |                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
( I* k' z! `6 T: X/ C                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
; a% Z2 }$ J- N' A                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ; m7 J9 w" D8 W
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
  M: t, d& B# E' C( \* }) _& a& t                     TRPRAKK"
1 P0 q0 `& `+ j3 k     promoter        complement(7703..8483)8 ]* z8 _3 t5 z0 P8 q) ]; X# ^
                     /note="CaMV35S2; CaMV 35S promoter, duplicated"
3 j8 n* N9 s& y4 Q     promoter        8631..8685
# z7 }2 n) O& x                     /note="PlacZ; lacZ promoter"
3 V9 |. N  S5 K9 z  v     CDS             8713..8946& p( |6 q/ Y, J+ x' h, F
                     /codon_start=1
. M, y5 F: o$ J+ ~) K                     /product="LacZ alpha fragment"
/ _5 |3 `& c7 S6 G- D                     /protein_id="AAF65322.1"# g0 o9 V* Z5 }( s/ Y- Q
                     /db_xref="GI:7638047"2 f0 Q) I& Q; g/ M# }
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
2 m% u! W# z' E1 U! I3 o$ ~                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"% A. `3 Z9 n$ A) i% O; K' Y
     misc_feature    8727..8783
# s. Z- U# H/ N9 B/ ]. R  E0 R. ^                     /note="pUC18 MCS; polylinker"
2 |/ _, J1 E: B4 V; H4 [$ C) e: `     misc_feature    9025..9050+ N7 P; [3 F  h' R8 N3 t1 f9 [
                     /note="right border T-DNA repeat"
/ k& N' ^. a& L& q7 k4 ?! {% yORIGIN      3 {" w( v& W: r: y3 R! O+ l4 G
        1 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc6 x- j% G; j1 m# G/ _
       61 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
+ s" M" r% j  ?9 j$ c4 d      121 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg4 C$ `+ ]$ g) ~, w; ]0 A
      181 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac0 H" b% l' F* ~, U
      241 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg+ g7 c+ M( j" Y# e( }8 z
      301 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc* U8 N7 |9 w2 U3 d+ M' v/ `8 }
      361 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc* V: g4 J- y  [. ~# r
      421 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
$ t+ K5 c. x- K6 X* Y      481 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
( h4 r. o( [  `* l      541 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc/ V( f, n! C) H" x6 Y( Q
      601 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc% i0 m* d* l0 J
      661 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg; L$ K1 f* j& l. h3 `- p
      721 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
& J! C# p, Y7 {( E* S6 T5 l      781 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
9 l3 M) q) W* t; ^- j( _5 }      841 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa$ d/ p, H/ B. D4 j0 A! f
      901 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta6 S, Q! C: d$ o' ?/ s6 P6 C) O: L
      961 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
3 A" {4 t8 M; W4 o( j5 _     1021 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct6 N8 z3 L  @6 ^- f$ D
     1081 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat" |; N  j" \8 K% E3 y4 h) `, n, V
     1141 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
2 g- a# L" y" E# {# n. n: K     1201 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg+ f* l+ {$ {3 I, u7 V" C$ V
     1261 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct& m5 l& K/ I, f9 r
     1321 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
% O0 w- a$ v- \" B8 D0 A     1381 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg: ]7 Y' M" W& F$ X; B- M0 B
     1441 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
8 o( F- T1 x+ o. F     1501 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
7 V+ ]. n" v2 M     1561 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca$ V: F- k- |2 \' `
     1621 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
9 C0 @% `( O. v# g2 U     1681 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta) R" ]1 c8 H$ b: g, l9 H
     1741 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc& J5 g" X" n; `' i
     1801 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt% j9 Z! W. E! s4 J9 q5 f: l
     1861 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc/ f  _) C) s( {' x, g1 ]
     1921 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt  Z5 T4 D, E+ r  V/ n' }/ ]0 Z9 A
     1981 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
% A; V/ Y6 N, q7 \     2041 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
8 ^- p- T* P8 p3 V( E     2101 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg3 j1 H' ]6 X: f- x$ M
     2161 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
0 J- }, E& v/ F     2221 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
; f0 t5 W9 h* @% @/ a, ^0 ]     2281 acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
+ d! `2 R2 ^6 g     2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg9 \+ B3 n& M+ e, i' i" V* R. g
     2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag2 h% R* G9 l4 j/ _! H" A
     2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
1 ]% S- {! Y& }: e# @9 N     2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc6 i2 r/ j, g" d9 [8 \# X
     2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
) U# n9 g0 i! ?% S     2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
$ l  S/ I, I- C& @3 w     2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
7 T# h4 u, C3 {     2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
7 U* w$ O3 n0 z" a# O     2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
) L/ n5 _8 r1 R% i9 D     2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg; }. Y5 @* M5 v
     2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg& I0 U$ e5 V* X
     3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc% [0 R3 J; x3 _# j7 d# J4 |
     3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc! k- Y* v. j- d( {3 Y
     3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
  q/ [1 a$ M2 }) h  N& b6 c. e# Q     3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt! L0 J1 _1 Z& b. D) N0 f
     3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
- j7 a  X7 p! S/ a) m     3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc. V5 ]( m" c+ H( ]2 y8 `
     3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
' ?6 n& q1 J- }3 N. O) P     3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
: ?. N: z* |9 Y$ T1 i/ P/ @     3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa7 i# J7 Z/ S' `
     3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
  m$ M2 Z; g% O" N# S. W) n+ y, G     3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc5 U4 a- @; e' O% S$ g* `  X
     3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct5 u( ^' l+ F: p+ i) `
     3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
' M1 L2 s) [9 _; b. X7 }4 ]4 s% @/ J     3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
2 C% q. w6 r) M1 p; r1 c) h. x( R; Y     3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
% `" T9 A- ~& C' p/ Q) L# a     3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
* \0 A1 ~0 P5 Z$ i1 G' l     3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc& Q3 x4 Q4 F* ]
     4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
( h5 N! d' i! C; d' d     4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
5 ]' j2 t0 n; x& f     4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag/ ?, c( F% |  J/ V  A+ C( {3 t
     4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa) Z/ l9 h. t0 s9 [1 B
     4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
$ B' T3 g# o2 X. o( t+ u# K     4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc: R3 O- L& Z9 V3 o7 ?, n
     4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
3 `. J( \! ?! k( w     4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt/ S! `6 F: e8 R) ~
     4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
6 W( {! T* ?% |: k3 a     4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc5 f( i, ^% T9 Z3 P6 S( O% _
     4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag' M$ E, \1 \# A9 F+ B" ]0 B
     4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
+ Y+ |$ A8 a8 z% @/ s     4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
) }/ ]' }7 ]6 S. D( k, _     4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag6 ]0 y7 O! H6 z6 L( V
     4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
2 V" ^0 |+ m( s3 ~6 b) f6 h5 N     4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg. J; N5 Q  y9 b  [  s, p
     4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg
3 F0 R3 X5 z- D; g- H5 k" b     5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt. O! b/ H7 y0 V, h! _" n0 F/ a
     5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca, q4 M. x2 a6 j- d2 x$ k. ]/ S/ W
     5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca- u* t4 q4 ]0 I+ J- u$ x
     5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc9 x5 P7 V% ]% a" |- J
     5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg. z- e9 I) b) `" b9 u* s. v% p
     5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
* b) ~+ y( M8 y) Y2 T) T     5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg& w) d4 L7 K; V
     5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg' k" [4 K+ S& r) [) \4 x  t% T
     5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
2 I" _" j7 J5 F! d* Q4 P3 ]5 d     5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg7 w+ I9 }5 ^2 a# q; m8 k& g
     5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta; W6 z$ a7 o6 i
     5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
2 g1 [) F3 s, k2 Z     5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg
: R$ w. H& A8 `" V9 b  }     5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg$ q6 l- u8 a/ ?: H( D
     5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata
# M% h3 ^! @: H" Z; m' p8 h* y     5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
9 h# y3 d7 n* z     6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc
* T% U, K/ I; g7 I1 h  N     6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
, R2 N+ c% P- H- F' }: B3 e, x$ m     6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
* H5 V" m" y! @: M2 j+ ]     6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
, B& C& d* z2 `4 I- K     6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc, d" ~4 G2 K3 S5 g
     6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
8 |: f0 L4 W$ T     6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa8 e& C) y: Q, s, R
     6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat! {; S$ G+ y# P4 S
     6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc  O( @3 Q6 e8 n8 t: e* D$ e3 j% I
     6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
1 v  D6 [1 b3 r2 i     6601 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
$ Y5 o. j, Y- A5 f. D4 y' n     6661 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
$ r# M# Y0 |* E4 _. f     6721 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
; ?6 _7 g3 w; S% I     6781 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc/ Y  K% y! I! h4 |" v6 Z6 P
     6841 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
* c% [6 {& h: C" N# T0 S1 _     6901 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
1 T2 V+ w$ F8 x8 F# H0 O     6961 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag6 I& g5 K: {9 g# R4 N* P/ \
     7021 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg+ l1 p( C. t% }
     7081 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
" b( }& Q" T% U/ Z2 g. [& c' u7 u     7141 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
; F  S0 A0 a9 \     7201 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg2 ]2 c: Y; w5 e( f1 [
     7261 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc. x0 @: T1 B1 {8 E% c2 {
     7321 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc+ W2 u) V6 M" T2 `+ Q2 Z% ~$ {; d
     7381 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact) U( Q; B# W% d$ g1 S* u
     7441 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
! [2 _: g/ K+ P" c" I     7501 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
! b/ [) Q1 A; P" |% l     7561 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg1 |# P+ F6 Z  `# _& {+ h! U
     7621 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc! S4 L5 P5 b0 d, F0 f9 [; ~
     7681 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg+ }) o8 J( |5 n3 F* |1 D
     7741 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
: E& x6 B( t* N6 B) p! d* P' ^     7801 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac+ s' O5 e2 r7 u7 S% H, H  I3 [8 c
     7861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
" m$ ~  I: I" e9 N     7921 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg. Y  f! e: Y% S% j+ Y5 g
     7981 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
: d% `; K- \8 v  j' v7 g4 m  V9 |6 e     8041 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
# h4 W5 z6 u4 {" Z# c* p/ ], I: n     8101 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
; B( ~& ?+ @' S1 j( e. _' X     8161 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
( H1 y$ W, O' }# q+ p- G- w     8221 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc9 t4 A# x7 L+ V4 L4 V; G' d+ ?
     8281 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg6 l* x7 f6 P( ?7 ~2 n
     8341 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt/ h7 \3 h( l! V, I& j6 L
     8401 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag3 S' W4 |" t* p$ h9 Z
     8461 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc
! }% G: t& S6 q* L# P" p! D     8521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
% k6 h* y+ @% p9 V, R5 L9 t     8581 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt4 B! u9 M, R$ Z* S$ [4 h4 O
     8641 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca4 f0 Z$ y) i  \+ \. P
     8701 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag. m, B) S' c1 q4 Q* E
     8761 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa) R1 X1 j( A0 B: ]7 U$ _/ {
     8821 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt& o! w5 m# s# B- ?  Y2 d( m0 T
     8881 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa/ {) C+ B# l2 r6 x% T* H! r) e2 z
     8941 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca0 h5 s( s! W0 |  {0 H* m; D0 ~
     9001 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac
( H/ h) S) }( Z; N     9061 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt- l: x* c+ ?* N' D5 m6 B
//