载体编号：P0063
载体名称：pACYC184
载体抗性：Tet、Chl
载体大小：4245bp
载体宿主：E.coli
载体特点：The 4245 bp plasmid, pACYC184, is compatible with pMB1- or ColE1-related plasmids and can therefore be used together with a pMB1- or ColE1-derivative within the same cell. pACYC184 contains: (1) the replicon rep responsible for the replication of plasmid (source - plasmid p15A); (2) tet gene, encoding tetracycline resistance protein (source - plasmid pSC101); (3) cat gene, coding for chloramphenicol acetyl transferase that confers resistance to chloramphenicol (source - transposon Tn9).
The circular sequence is numbered such that 1 is the first G of the unique EcoRI site GAATTC and the count increases first through the 5'-terminal part of cat gene, the p15A material, the tet gene and finally through the 3'-terminal part of cat gene. The map shows enzymes that cut pACYC184 DNA once. Enzymes produced by Fermentas are shown in blue. The coordinates refer to the position of first nucleotide in each recognition sequence.
The exact position of genetic elements is shown on the map (termination codons included). The indicated rep region is sufficient to promote replication. DNA replication initiates at position 846 (+/- 1) and proceeds in the direction indicated.
Derivatives of p15A are normally present in a somewhat lower copy number than pMB1 derivatives and cannot be amplified to the same extent as pMB1 derivatives. Due to the presence of the cat gene on pACYC184, amplification of this plasmid should be performed using spectinomycin.
网站链接：http://www.fermentas.com/techinfo/nucleicacids/mappacyc184.htm
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