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【质粒图谱】pS1质粒图谱及相关信息
pS1质粒图谱及相关信息 图谱库ID：G0640 质粒名称: pS1
相关说明：Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Cloning vector with the multiple cloning site expanded by inserting an oligonucleotide at the SmaI site of pUC19, thereby generating an amber mutation in the lacZ' coding sequence. [1] The plasmid contains the following restriction sites (bp from 0): DrdI--91, 992; KasI--233; PvuI--276, 2150; EcoRI--396; NotI--418; NsiI--493; HindIII--531; SapI--767; BsaI--1850; SspI--2585. (personal communication) The order of the major features in this plasmid is: KasI - 3' lacZ'amb1 - EcoRI/MCS/HindIII - 5' lacZ'amb1 - SapI - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--1.9, 0.9; EcoRI--2.8; HindIII--2.8; NotI--2.8; SspI--2.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM109. Related vectors: pUC19.pS1图谱： 
特征序列区位： M13_forward20_primer | | 379 - 395 | AmpR_promoter | | 2640 - 2612 | M13_pUC_fwd_primer | | 364 - 386 | lacZ_a | | 398 - 250 | pGEX_3_primer | | 51 - 29 | Orf2 | | 2570 - 1710 | M13_reverse_primer | | 563 - 545 | Ampicillin | | 2570 - 1710 | lac_promoter | | 627 - 598 | pBR322_origin | | 1555 - 936 | M13_pUC_rev_primer | | 584 - 562 |
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