pNKY51质粒图谱及相关信息
图谱库ID：G0565
质粒名称：pNKY51
载体类型：Yeast expression
载体抗性：Ampicillin
培养条件：Grow in standard E. coli @ 37C
其他信息：Provided In DH5a
相关说明：pNKY51 (hisG direct repeats separated by URA3): pNKY5 1 was built in four steps. (1) The backbone, pNKY3 was made by deleting the 2micron DNA from YEP24 with EcoRI and by inserting a BglII linker at the remaining EcoRI site (EcoR1 site is regenerated). (2) A 1.1kb BglII-BamHI fragment of pNK294 bearing Salmonella hisG DNA was inserted into the BglII site of pNKY3 to form pNKY49. (3) The same 1.1-kb hisG fragment as in (2) was inserted at the BamHI site of pNKY49 to form pNKY50. (4) The EcoRI site at the 5’ end of URA3 in pNKY50 was destroyed by fill in and ligation reactions. The resulting plasmid, pNKY51, contains the 3.8 kb hisG-URA3-hisG fragment that can be gel isolated by a BglII and BamHI digest.
pNKY51图谱：
map:
作者
