Re: 【求助】RNAi载体中的intron的选择
Length and Sequence of the Loop Linking Sense and Antisense Strands of Hairpin siRNA
Various research groups have reported successful gene silencing results using hairpin siRNAs with loop size ranging between 3 to 23 nucleotides (4, 6-9, 11). The following is a summary of loop size and specific loop sequences used by various research groups:
Loop Size (# of Nucleotides) Specific Loop Sequence Reference
3 AUG 4
3 CCC 7
4 UUCG 5
5 CCACC 7
6 CTCGAG 2
6 AAGCUU 2
7 CCACACC 7
9 UUCAAGAGA 6
23 Not reported 9
我的课题也是RNAi相关的,http://www.ambion.com/techlib/tb/tb_506.html,上面的内容是从这个网页复制下来的,希望有用.
