Gene/insert name: , s1 c4 T# P4 I) ~, j5 X
| $ D6 v' J" W9 z7 A
| beta-gal ) @+ Z1 v) v3 U& J& ]* q. b5 R3 }$ s
|
( Z8 d) j: s* V( _% f |
Insert size (bp):
0 u* [) r! o3 g$ t | + Y5 C) H: o0 N' @7 M, R" F
| Unknown # Q3 u) |4 j) m
| " E2 O: F* u. a: \- u8 g8 N% p
|
Species of gene(s): ( u0 m' ], H# V) }- f- G. V
|
- h' Z9 P/ j3 A$ j | E. coli
0 Y- C( r+ |) C% w, r% d& h& \ |
+ j+ c0 r) g% E |
Relevant mutations/deletions: / L4 g) y& v$ N! ~& K9 `
| - ~+ g/ k& c2 o9 ~" D2 O+ d/ W
| P (proline) at amino terminus of beta-gal & j+ `5 P. E. `7 O
|
% i2 u3 U5 r9 a$ L |
Fusion proteins or tags:
- v! I _0 ^# [2 ?. ~( R/ Z | 4 }. A2 z$ p F, g6 c
| Ubiquitin $ j8 N2 k. o+ `$ B: p6 U4 d
|
7 r, A7 ~' Q* | |
Terminal: 4 L2 `1 ~! Q! E1 G' V9 X
| 0 x: W: e* |, K1 `* k0 Y( ?# D
| N terminal on backbone
6 V! ` {* {8 F- K. A' } |
7 N" U; ~* q# y- t |
Vector backbone: . l- C% o3 |# v7 |
| M8 e% H, [0 C: c* @( z3 S
| pUB23 (Search Vectorpedia)
, L: q4 y* @8 [& I |
8 }' g& m& w- X3 s# D |
Type of vector:
$ K+ m( t6 l$ y Z; s |
/ ], D2 A( T( l+ C1 w5 C | Yeast expression % M' i9 \0 K' i6 ~4 f- _
| 4 n3 F8 s# g' ~9 d
|
Backbone size (bp): 1 X7 |: c/ ^' J& B0 ], [- A
|
; _' F; d2 C7 c; A4 l | 10500 + @, V% F* D5 R4 C+ |: E
|
+ V. q, Q/ x: x |
5' Sequencing primer: 7 m% k( z8 j( X" {1 s, \) e
| 1 }5 k% I1 N: ^8 W0 n
| lacZ-R (List of Sequencing Primers) ; J- a4 j4 n+ Z% z3 N4 g
|
1 d9 ~6 _( i1 p0 y |
Bacteria resistance: * O4 `; z6 y, A
|
; o$ l/ s) D5 h" g) P7 E1 P | Ampicillin 1 f7 U4 t; S5 K9 N- \
| 5 ^! i1 ?2 [ I- j
|
High or low copy: 1 E6 y" r. L3 ^) g& i" F% R% }* s) o
|
% j7 @! D& c1 e2 O1 _ | High Copy
. q% b0 G$ ?0 x6 @ |
$ V: x6 Z% k! V, J& X5 E* ^ |
Grow in standard E. coli @ 37C:
4 \$ J2 F1 K" t; ~ |
2 ^' y3 F' q& Z5 F" o- t! O | Yes . W. h) Q4 Q' ~6 Q$ j
| 0 t) g. L9 u$ ~0 b8 Q: v9 Q
|
Selectable markers: % U) p% A+ A% N. R+ d: _6 {
|
- z, l+ p! W* B9 `/ c | URA3
' |1 r( k8 T3 b( S, H: P' G, `2 z | % \0 I6 v* a$ z0 o
|
Sequence:
2 Z6 D6 G* S% x$ R* s |
( C9 f2 D! _! w6 C; X {/ v% I( q* @ | View sequence 2 B+ x( |0 J7 |$ V2 B6 F. w
|
- o) P; G+ r$ G- V. W+ Y |
Author's Map: d" A& I0 C; I5 A: k1 M
| / o! S9 ~. e$ p2 j
| View map ; z! T8 y0 E7 M+ p
|
5 F: t; w8 A5 s; D |
Plasmid Provided In: l7 D. q$ Y& c" l5 [" }6 J+ I
|
/ ^7 g2 T2 u5 u5 Y1 h | DH5a
2 ]0 r6 z+ e7 e# f: C: F( }% ]3 V |
5 X! y& e6 q9 g( e" r, y |
Principal Investigator: / R- A& W, b& w' Z. P7 K3 A+ A
|
+ z; f: Y Z, I& n& C$ V8 k1 r1 o | Alexander Varshavsky 6 z% ?& t% F- C+ |) U
|
( j3 g- q0 j/ M; q% J |
Terms and Licenses:
$ u- \3 O o9 E9 F2 A! r* D% y4 ] | A5 x; w9 @% l
| MTA . ]6 G. u) ]+ b; r3 d# `: C& W, {
|
7 |* ~, A7 t& [ M& f* F& B |
