pRS405质粒图谱及相关信息
图谱库ID：G0192
质粒名称：pRS405
载体类型：phagemid
载体抗性：LEU2, ampR
培养条件：LB Medium with 50 mcg/ml ampicillin；Temperature: 37℃
相关说明：YI-type (integrating) shuttle vector；A YI-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
pRS405图谱：
特征序列区位：
pBluescriptKS_primer | | 3143 - 3159 |
M13_pUC_fwd_primer | | 3059 - 3081 |
T7_promoter | | 3100 - 3118 |
M13_forward20_primer | | 3074 - 3090 |
lacZ_a | | 3088 - 2945 |
f1_origin | | 2912 - 2606 |
LEU2 | | 1757 - 663 |
pBluescriptSK_primer | | 3210 - 3194 |
pGEX_3_primer | | 51 - 29 |
Orf0 | | 5307 - 4447 |
Orf2 | | 1769 - 663 |
T3_promoter | | 3265 - 3246 |
M13_reverse_primer | | 3300 - 3282 |
M13_pUC_rev_primer | | 3321 - 3299 |
pBR322_origin | | 4292 - 3673 |
Ampicillin | | 5307 - 4447 |
AmpR_promoter | | 5377 - 5349 |
