Prs316质粒图谱及相关信息
图谱库ID:G0186
质粒名称:pRS316
载体类型:phagemid
载体抗性:URA3, ampR
培养条件:LB Medium with 50 mcg/ml ampicillin;Temperature: 37℃
相关说明:YC-type (centromeric) shuttle vector;plasmid shuffling;vector containing primer sites useful for sequencing;vector permitting RNA synthesis in vitro;vector permitting production of single-stranded DNA;vector permitting visual detection of recombinants;YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
pRS316图谱:

特征序列区位:
pBluescriptSK_primer | | 2017 - 2033 |
pBluescriptKS_primer | | 2084 - 2068 |
M13_reverse_primer | | 2166 - 2148 |
T7_promoter | | 1966 - 1984 |
M13_forward20_primer | | 1943 - 1959 |
M13_pUC_fwd_primer | | 1928 - 1950 |
lacZ_a | | 1962 - 1814 |
f1_origin | | 1781 - 1475 |
pBR322_origin | | 3158 - 2539 |
URA3 | | 411 - 1211 |
URA3_promoter | | 196 - 409 |
pGEX_3_primer | | 51 - 29 |
Orf2 | | 411 - 1214 |
Orf0 | | 4173 - 3313 |
Ampicillin | | 4173 - 3313 |
M13_pUC_rev_primer | | 2187 - 2165 |
T3_promoter | | 2131 - 2112 |
AmpR_promoter | | 4243 - 4215 |
CEN6_ARS4 | | 4305 - 4823 |