pGEX-KN质粒图谱及相关信息
图谱库ID:G0138
质粒名称:pGEX-KN
载体类型:phagemid
载体抗性: ampR
培养条件:LB Medium with 50 mcg/ml ampicillin;Temperature: 37℃
相关说明:encodes an epitope tag for protein isolation or monitoring;expression vector
vector permitting construction of fusion proteins;Expression vector for rapid purification of fusion proteins that contain no amino terminal extensions after thrombin cleavage. The amino acid after the initiator methionine must be charged.
pGEX-KN图谱:

特征序列区位:
lacI | | 3306 - 4397 |
Ampicillin | | 1374 - 2234 |
AmpR_promoter | | 1304 - 1332 |
pGEX_3_primer | | 1038 - 1016 |
pBR322_origin | | 2389 - 3008 |
pGEX_5_primer | | 869 - 891 |
GST | | 258 - 965 |
M13_pUC_rev_primer | | 224 - 246 |
tac_promoter | | 184 - 212 |
Orf2 | | 3438 - 4397 |
Orf2 | | 1374 - 2234 |
Orf2 | | 258 - 965 |
lac_promoter | | 4446 - 4475 |
M13_pUC_rev_primer | | 4489 - 4511 |
M13_reverse_primer | | 4510 - 4528 |
lacZ_a | | 4537 - 4692 |
M13_forward20_primer | | 4556 - 4540 |
M13_pUC_fwd_primer | | 4571 - 4549 |