pfXblue质粒图谱及相关信息
图谱库ID:G0135
质粒名称:pfXblue
载体类型:plasmid
载体抗性: ampR
培养条件:LB Medium with 50 mcg/ml ampicillin;Temperature: 37℃
相关说明:expression vector;vector permitting construction of fusion proteins;vector permitting visual detection of recombinants;vector useful for cloning PCR products;Expression vector permitting the production of a fusion protein that can be trimmed by cleavage with the coagulation factor Xa protease. A modified lacZ' allows blue/white screening of colonies.
pfXblue图谱:

特征序列区位:
lacZ_a | | 1023 - 1166 |
M13_pUC_fwd_primer | | 1045 - 1023 |
Ampicillin | | 3610 - 2750 |
M13_forward20_primer | | 1030 - 1014 |
M13_reverse_primer | | 1603 - 1585 |
M13_pUC_rev_primer | | 1624 - 1602 |
PL_promoter | | 346 - 589 |
Orf1 | | 3610 - 2750 |
lac_promoter | | 1667 - 1638 |
Orf0 | | 766 - 1446 |
pBR322_origin | | 2595 - 1976 |