【质粒图谱】pDO17质粒图谱及相关信息

pDO17质粒图谱及相关信息

图谱库ID：G0110

质粒名称：pDO17

载体类型：cosmid

载体抗性：ampR,kanR,tetR

培养条件：Medium(see below)plus ampicillin (50.0 mcg/ml) plus kanamycin (20.0 mcg/ml) Medium：Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L；Temperature:30℃

相关说明：vector containing primer sites useful for sequencing;vector for generating nested deletions of cloned inserts;vector permitting visual detection of recombinants;Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing. To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

pDO17图谱：

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