pRS428质粒图谱及相关信息
图谱库ID：G0032
质粒名称：pRS428
载体类型：Yeast expression
载体抗性：Ampicillin
培养条件：Grow in standard E. coli @ 37C
其他信息：Provided in DH5a，High Copy
相关说明：p4339 + pRS420 --> pRS428. Insert is NatMX (clonNAT resistance) from EcoRI-cut p4339. Backbone is NcoI-cut pRS420 (2 micron plasmid).
pRS428图谱：
特征序列区位：
pGEX_3_primer | | 51 - 29 |
f1_origin | | 1933 - 1627 |
M13_pUC_fwd_primer | | 2080 - 2102 |
M13_forward20_primer | | 2095 - 2111 |
lacZ_a | | 2109 - 1966 |
T7_promoter | | 2121 - 2139 |
pBluescriptKS_primer | | 2164 - 2180 |
pBluescriptSK_primer | | 2231 - 2215 |
T3_promoter | | 2286 - 2267 |
M13_reverse_primer | | 2321 - 2303 |
M13_pUC_rev_primer | | 2342 - 2320 |
lac_promoter | | 2385 - 2356 |
pBR322_origin | | 3313 - 2694 |
Ampicillin | | 4328 - 3468 |
Orf2 | | 4328 - 3468 |
AmpR_promoter | | 4398 - 4370 |
2micron2_origin | | 5431 - 4460 |
FRT | | 5470 - 5423 |
