p079 pPNT6质粒图谱及相关信息
图谱库ID：G0003
质粒名称：p079 pPNT6
载体类型：Mammalian expression,Cre/Lox
载体抗性：Ampicillin
培养条件：Grow in standard E. coli @ 37C
其他信息：Provided in DH5a，High Copy
相关说明：Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.
p079 pPNT6图谱：
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特征序列区位：
loxP | | 52 - 85 |
MSCV_rev_primer | | 166 - 143 |
Neomycin | | 618 - 1418 |
Orf2 | | 618 - 1421 |
loxP | | 1916 - 1949 |
MSCV_rev_primer | | 2082 - 2059 |
Orf2 | | 2619 - 3749 |
TK_PA_terminator | | 3734 - 4004 |
lacZ_a | | 4827 - 4970 |
M13_forward20_primer | | 4834 - 4818 |
M13_pUC_fwd_primer | | 4849 - 4827 |
pGEX_3_primer | | 5162 - 5184 |
AmpR_promoter | | 5343 - 5371 |
Orf0 | | 5413 - 6273 |
Ampicillin | | 5413 - 6273 |
pBR322_origin | | 6428 - 7047 |
lac_promoter | | 7356 - 7385 |
M13_pUC_rev_primer | | 7399 - 7421 |
M13_reverse_primer | | 7420 - 7438 |
T3_promoter | | 7455 - 7474 |
