载体编号：P0059
载体名称：pPIC9K
载体抗性：Amp、Kan
载体大小：9.3Kbp
载体宿主：酵母表达、大肠杆菌
载体特点：The vector pPIC9K contains the kanamycin resistance gene for in vivo screening of multiple copy inserts and secretes recombinant proteins to the medium. pPIC9K is functional in Pichia strains GS115 and KM71. Other details are:
.9276 bp fusion vector
.Four unique restriction sites for cloning in frame with the á-factor secretion signal: SnaB I, EcoR I, Avr II, Not I
.Secreted expression of your gene using the á-factor secretion signal
.For expression, your gene must be cloned in frame with the initiation codon of the signal sequence
.HIS4 selection in Pichia
.For insertion at AOX1 in GS115 or KM71, linearize with Sac I (generates His+ Mut+ in GS115 and His+ MutS in KM71)
.For insertion at HIS4, linearize with Sal I (generates His+ Mut+ in GS115 and His+ MutS in KM71)
.For gene replacement at AOX1 in GS115, linearize with Bgl II (generates His+ MutS) Please see page 30 for alternate restriction sites if your insert DNA has a Bgl II, Sac I, or Sal I site.
网站链接：http://www.invitrogen.com/content/sfs/manuals/ppic3.5kpao_man.pdf
此资源由“Papaya”酷友共享，特此感谢！
