载体编号：P0058
载体名称：pPIC3.5K
载体抗性：Amp、Kan
载体大小：9.0Kbp
载体宿主：酵母表达、大肠杆菌
载体特点：pPIC3.5K is a plasmid designed to allow you to identify in vivo multiple integrations of your gene in the Pichia genome. Other details about pPIC3.5K are provided below:
.9004 bp vector
.Five unique restriction sites in the multiple cloning site: BamH I, SnaB I, EcoR I, Avr II, Not I
.Intracellular expression of your gene
.Requires an initiating ATG codon in a Kozak consensus sequence for proper translation initiation of your gene (Cavener and Stuart, 1991; Kozak, 1987; Kozak, 1990)
.HIS4 selection in Pichia
.For insertion at AOX1 in GS115 or KM71, linearize with Sac I (generates His+ Mut+ in GS115 and His+ MutS in KM71)
.For insertion at HIS4, linearize with Sal I (generates His+ Mut+ in GS115 and His+ MutS in KM71)
.For a gene replacement at AOX1 in GS115, linearize with Bgl II (generates His+ MutS) Please see page 29 for alternate restriction sites if your insert DNA has a Bgl II, Sac I, or Sal I site.
网站链接：http://www.invitrogen.com/content/sfs/manuals/ppic3.5kpao_man.pdf
此资源由“Papaya”酷友共享，特此感谢！
