http://pga.mgh.harvard.edu/primerbank/index.html

这是一个免费的PCR引物库，有180 000个引物可供查询和选择，是基于一篇发表文章里的算法计算出来的，在该文章中以实验验证了112条引物，证实有效率为98.2％。大家可以看看有没有自己感兴趣的基因引物。

PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains about 180,000 primers covering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, PrimerBank ID or Keyword (gene description). 

The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency. Up to now, we have tested over a thousand primers and the design success rate is > 99%. 

Publication:

Xiaowei Wang and Brian Seed (2003) A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Research 31(24): e154; pp.1-8.

再截个图看看，界面很简洁：

 

谢谢纤夫推荐!奖.....

[quote user="纤夫"]

PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains about 180,000 primerscovering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, PrimerBank ID or Keyword (gene description). 

[/quote]

 

可惜靶基因都是人和鼠基因组的。。

[quote user="Jack"]

可惜靶基因都是人和鼠基因组的。。

[/quote]

 

呵呵，主攻领域不同。这个算是Viroligo的补充吧。

嘉奖 一杠三星

primer bank 中的引物是不是不能用在RT－PCR中啊？

多谢！对我试验帮助非常大啊!

人和小鼠的，正好！