载体编号：P0049
载体名称：PBR322
载体抗性：Amp、Tet
载体大小：4361bp
载体宿主：E.coli
载体特点：The plasmid pBR322 is one of the most commonly used E.coli cloning vectors. pBR322 is 4361 bp in length and contains: (1) the replicon rep responsible for the replication of plasmid (source - plasmid pMB1); (2) rop gene coding for the Rop protein, which promotes conversion of the unstable RNA I - RNA II complex to a stable complex and serves to decrease copy number (source - plasmid pMB1); (3) bla gene, coding for beta-lactamase that confers resistance to ampicillin (source - transposon Tn3); (4) tet gene, encoding tetracycline resistance protein (source - plasmid pSC101).
The circular sequence is numbered such that 1 is the first T of the unique EcoRI site GAATTC and the count increases first through the tet gene, the pMB1 material, and finally through the Tn3 region. The map shows enzymes that cut pBR322 DNA once. Enzymes produced by Fermentas are shown in blue. The coordinates refer to the position of first nucleotide in each recognition sequence.
The exact position of genetic elements is shown on the map (termination codons included). The bla gene nucleotides 4153-4085 (complementary strand) code for a signal peptide. The indicated rep region is sufficient to promote replication. DNA replication initiates at position 2533 (+/- 1) and proceeds in the direction indicated. Plasmids carrying the pMB1 and ColE1 replicons are incompatible, but they are fully compatible with those carrying the p15A replicon.
网站链接：http://www.fermentas.com/techinfo/nucleicacids/mappbr322.htm
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