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大 中
小 发表于 06-9-23 00:41 只看该作者
比利时标准生物品收藏中心简介
 不错的保藏中心，网址 http://bccm.belspo.be ，以下是他们基因保藏中心的简介： | Holding
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950 recombinant plasmids that can be used as valuable tools for applied and fundamental molecular biological, immunological and biomedical research. These plasmids represent two groups: - The "vector" group consists of general purpose cloning vectors and highly sophisticated expression vectors designed for cloning and expression of a variety of coding sequences in prokaryotes, yeasts, fungi or higher eukaryotic (insect, mammalian and plant) cells.
- The group "cloned genes" consists of constructs that carry cloned gene sequences coding for a variety of proteins from eukaryotic as well as prokaryotic sources, such as cytokines, receptors, receptor adaptors, inhibitors, transcription factors, reporter proteins, influenza proteins, phage proteins, tobacco and Arabidopsis proteins, etc.
All these plasmids are described in full molecular detail, whenever possible at the DNA sequence level. Functional features, such as cloned genes, promoters, ribosome binding sites, transcription terminators and other items are displayed on a graphical, circular map, and described in an accompanying data sheet. The latter also contains detailed information on the construction of the plasmid, its application areas, required host strains and other useful features. The compiled nucleotide sequence file is suitable for several DNA analysis packages, allowing restriction site analysis, translation, homology searches, etc. BCCM/LMBP plasmids have been submitted to the following quality control tests: purity, viability and authenticity (analysis of the restriction enzyme pattern). - A selection of recommended or required bacterial host strains.
- 18 unique DNA libraries, from fungal to human origin. The DNA libraries are oligo(dT) and/or random hexamer primed, and most often cloned in an oriented way in an expression cassette.
Services
All assignments, with exception of deposits of microbial resources for public access, are treated strictly confidential. -
Public deposit BCCM/LMBP accepts for public deposit: - plasmids
- bacterial host strains
- DNA libraries
Before sending material, it is advisable to contact BCCM/LMBP to make sure that the material to be deposited is within its scope and technical capability. Deposits for public access are without costs for the depositor.
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Safe deposit BCCM/LMBP accepts for safe deposit: - any kind of natural or recombinant plasmid or genetic material (e.g. RNA, oncogenes), either in a purified form or within a host
- natural or genetically modified animal cell lines, including human cell lines and hybridomas
Before sending material, it is advisable to contact BCCM/LMBP to make sure that the material to be deposited is within its scope and technical capabilities. For related costs see pricelist.
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Patent deposit BCCM/LMBP accepts for patent deposit: - any kind of natural or recombinant plasmid or genetic material (e.g. RNA, oncogenes), either in a purified form or within a host
- natural or genetically modified animal cell lines, including human cell lines and hybridomas
Before sending material, it is advisable to contact BCCM/LMBP - to make sure that the material to be deposited is within its scope and technical capabilities
- to be informed of the necessary form and quantity of the material
Upon deposit, the depositor must complete the international Accession Form BCCM/LMBP/BP/1 as well as the bilateral contract Form BCCM/LMBP/DBT1.
Once BCCM/LMBP has accepted the material and confirmed its viability and purity, it will send to the depositor or to the party designated on form BP/1, the completed international Forms BCCM/LMBP/BP/4 (receipt of an original deposit) and BCCM/LMBP/BP/9 (viability statement). These documents constitute the proofs of the deposit. See also the detailed Manual for the deposit of Biological Material for Patent Purposes under the Budapest Treaty. For related costs see pricelist.
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Distribution of biological material Resources of the public collection are accessible to the scientific community under the conditions of the BCCM Material Transfer Agreement (MTA), if necessary amended with additional conditions possibly already attached to the biological material.
For more information on ordering microbial resources click here.
Consult the BCCM/LMBP plasmid catalogue or the BCCM/LMBP DNA library catalogue to search the resources you require. Resources of the safe deposit collection are not catalogued and are only available to the depositor or to third parties with the written authorization of the depositor.
For related costs see pricelist. The distribution of samples of biological material deposited under the Budapest Treaty for patent purposes depends on the authorization by the depositor or an entitled Industrial Property Office. Patent deposits are not catalogued.
For related costs see pricelist. Accession, control, preservation, storage and supply of the biological material are ISO 9001:2000 certified.
Research interests
- Basic molecular biology and cell biology: gene expression studies in homologous and heterologous hosts. Several techniques are routinely used, including mutagenesis, construction of DNA libraries, direct expression screening, PCR and DNA sequencing;
- Heterologous gene expression in prokaryotes, yeasts, fungi, insect cells or higher animal cells and purification of the corresponding proteins;
- Biomedical research, especially related to cancer and inflammatory diseases;
- Immunology.
Organisation chart
Further information & contact
Promoter: Prof. Dr Ir Roland Contreras Scientific Advisor: Prof. Dr Erik Remaut Manager: Lic. Martine Vanhoucke Curator: Lic. Martine Vanhoucke Contact: Note: BCCM/LMBP holidays 2006Lic. Martine Vanhoucke BCCM/LMBP Department of Molecular Biology Ghent University 'Fiers-Schell-Van Montagu' Building Technologiepark 927 B-9052 Zwijnaarde Phone: +32-9-33.13.843 Fax: +32-9-33.13.504 E mail: bccm.lmbp@dmbr.UGent.be |
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