Protein production and purification.
Nat Methods. 2008 Feb;5(2):135-46
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
PMID: 18235434
就是这篇了，本版之前还组织过中文化的，就在置顶里，文章是不错，都是经验之谈。
[ 本帖最后由 tangboyun 于 08-10-5 13:48 编辑 ]
