基因酷，您好！我是基因酷valeron，我已认真阅读《资源保证协议》，并同意签署。我欲预存：
酵母双杂交系统（Clontech）：pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam
腺病毒系统（Stratagene）：pAdEasy-1，pShuttle-CMV
酵母表达载体（Invitrogen）：pGAPZα A，pPICZα A
哺乳动物表达载体（Invitrogen）：pcDNA4/HisMax B，pBudCE4.1这10个质粒。
我预共享的质粒基本信息：
载体名称：pCL1
载体抗性：amp
载体大小：15.3kb
载体宿主：E. coli/yeast
载体特点：Matchmaker Two-Hybrid System 3的独立正对照。pCL1 (Fields & Song, 1989) is a positive control plasmid that encodes the fulllength,
wild-type GAL4 protein. The GAL4 protein activates reporter genes under the control of a
GAL4-responsive element (UASG). Thus, pCL1 is used as a positive control for the transcription assay
in GAL4-based MATCHMAKER Two-Hybrid Systems. This vector is a derivative of YCp50 referenced
in Fields & Song, 1989. pCL1 has not been sequenced.
网站链接：
http://www.clontech.com/images/pt/dis_manuals/PT3062-1.pdf page 15
载体名称：pGBKT7-53
载体抗性：kan
载体大小：8.3kb
载体宿主：E. coli/yeast
载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced
网站链接：
http://www.clontech.com/images/pt/PT3183-1.pdf page 43
载体名称：pGADT7-T
载体抗性：amp
载体大小：10.0kb
载体宿主：E. coli/yeast
载体特点：与pGBKT7-53构成Matchmaker Two-Hybrid System 3的双杂交正对照。pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced
网站链接：
http://www.clontech.com/images/pt/PT3183-1.pdf page 44
载体名称：pGBKT7-Lam
载体抗性：kan
载体大小：7.9kb
载体宿主：E. coli/yeast
载体特点：Matchmaker Two-Hybrid System 3的负对照。pGBKT7-Lam is a negative control plasmid that encodes a fusion
of the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank
Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed at
BD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the background
that is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced
网站链接：
http://www.clontech.com/images/pt/PT3955-1.pdf page 56
载体名称：pAdEasy-1
载体抗性：amp
载体大小：33.5kb
载体宿主：E. coli/mammals
载体特点：AdEasy腺病毒系统的骨架质粒
网站链接：
http://www.stratagene.com/vectors/maps/pdf/pAdEasy-1.pdf
载体名称：pShuttle-CMV
载体抗性：kan
载体大小：7.5kb
载体宿主：E. coli/mammals
载体特点：AdEasy腺病毒系统的克隆载体，CMV启动子。
网站链接：
http://www.stratagene.com/vectors/maps/pdf/pShuttle-CMV.pdf
载体名称：pGAPZα A
载体抗性：zeo
载体大小：3.1kb
载体宿主：E. coli/yeast
载体特点：在毕赤酵母中组成型表达分泌型蛋白
网站链接：
http://tools.invitrogen.com/content/sfs/vectors/pgapza_map.pdf
载体名称：pPICZα A
载体抗性：zeo
载体大小：3.6kb
载体宿主：E. coli/yeast
载体特点：在毕赤酵母中甲醇诱导下表达分泌型蛋白
网站链接：
http://tools.invitrogen.com/content/sfs/vectors/ppiczalpha_map.pdf
载体名称：pcDNA4/HisMax B
载体抗性：amp/zeo
载体大小：5.3kb
载体宿主：E. coli/mammals
载体特点：在哺乳动物细胞内高效表达目的蛋白，提供用于纯化的融合标签等
网站链接：
http://tools.invitrogen.com/content/sfs/vectors/pcdna4hismax_map.pdf
载体名称：pBudCE4.1
载体抗性：zeo
载体大小：4595bp
载体宿主：E. coli/mammals
载体特点：在哺乳动物细胞中用两个不同的启动子高效组成型表达目的蛋白
网站链接：
http://tools.invitrogen.com/content/sfs/vectors/pbudce4_1_map.pdf
请予以回复！
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本帖最后由 valeron 于 08-8-23 01:07 编辑 ]
