pGEMEX-1

载体名称：pGEMEX-1 载体抗性：amp 载体大小：3993 载体宿主：DH5alpha, JM109 载体特点：The pGEMEX® Vectors are T7 expression vectors that can be used for cloning, as templates for in vitro transcription and for production of single-stranded DNA (ssDNA). Expression from the pGEMEX® Vectors is based on the T7 expression system( a) developed by Studier (1), which uses a convenient vector/host combination for high-level expression of cloned genes in vivo. Sequences cloned into the pGEMEX® Vectors are expressed as T7 gene 10 fusion proteins in JM109(DE3)(a) or BL21(DE3)pLysS(c) host strains containing an inducible gene for T7 RNA polymerase. JM109(DE3) is a specially constructed host strain that contains an IPTGinducible gene for T7 RNA polymerase; JM109(DE3) and JM109 are supplied with the pGEMEX® Vectors. The pGEMEX®-1 and pGEMEX®-2 Vectors differ by only two base pairs resulting in a shift in the reading frame (Figure 1). The bacteriophage T7 gene 10 leader peptide (260 amino acids) is expressed very efficiently in this host and can accumulate to greater than 50% of total cell protein in three hours or less. Typical levels of fusion protein production are on the order of 10mg per liter of induced culture. The pGEMEX® Vectors are unique in that they contain three RNA polymerase promoters. The T7 promoter, along with the gene 10 ribosome binding site, is positioned upstream from the gene 10 leader fragment. A T7 transcription terminator is incorporated downstream from the multiple cloning region and functions to end transcription The pGEMEX® Vectors(a,b) are T7 expression vectors that can be used for cloning, as templates for in vitro transcription and for production of single-stranded DNA (ssDNA). Expression from the pGEMEX® Vectors is based on the T7 expression system( a) developed by Studier (1), which uses a convenient vector/host combination for high-level expression of cloned genes in vivo. Sequences cloned into the pGEMEX® Vectors are expressed as T7 gene 10 fusion proteins in JM109(DE3)(a) or BL21(DE3)pLysS(c) host strains containing an inducible gene for T7 RNA polymerase. JM109(DE3) is a specially constructed host strain that contains an IPTGinducible gene for T7 RNA polymerase; JM109(DE3) and JM109 are supplied with the pGEMEX® Vectors. The pGEMEX®-1 and pGEMEX®-2 Vectors differ by only two base pairs resulting in a shift in the reading frame (Figure 1). The bacteriophage T7 gene 10 leader peptide (260 amino acids) is expressed very efficiently in this host and can accumulate to greater than 50% of total cell protein in three hours or less. Typical levels of fusion protein production are on the order of 10mg per liter of induced culture. The pGEMEX® Vectors are unique in that they contain three RNA polymerase promoters. The T7 promoter, along with the gene 10 ribosome binding site, is positioned upstream from the gene 10 leader fragment. A T7 transcription terminator is incorporated downstream from the multiple cloning region and functions to end transcription. 网站链接：http://www.promega.com/vectors/discontinued_vectors.htm 此资源由"haozhm"酷友提供，特此感谢！