载体名称：pcdna3.1/v5-his B/c
载体大小：5.5kb
载体抗性：Amp
载体宿主：E. coli & mammalian cells
载体特点：pcDNA.3.1/V5-His A, B, and C are 5.5 kb vectors derived from pcDNA.3.1(+) and designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
1. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide
2. Human cytomegalovirus immediate-early (CMV) promoter for high-level expression
in a wide range of mammalian cells
3.Episomal replication in cell lines that are latently infected with SV40 or that express
the SV40 large T antigen (e.g. COS7).
网站链接：
www.invitrogen.com
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