Nature method关于RNAi的一些好文，免费全文哦。

This set of eight especially commissioned articles is intended to constitute a user’s guide to RNA interference in mammalian cells. The articles are available after free registration until the end of November. Check the content of this site during the upcoming months as it will be updated with new material each month.

Foreword
Focus on RNA interference - A user's guide
Focus on RNA interference p669
Veronique Kiermer
doi:10.1038/nmeth0906-669
Full text | PDF (112K)

Perspectives
Focus on RNA interference - A user's guide
On the art of identifying effective and specific siRNAs pp670 - 676
Yi Pei & Thomas Tuschl
Published online: 23 August 2006 | doi:10.1038/nmeth911
Abstract | Full text | PDF (215K)


Focus on RNA interference - A user's guide
Enhancing and confirming the specificity of RNAi experiments pp677 - 681
Bryan R Cullen
Published online: 23 August 2006 | doi:10.1038/nmeth913
Abstract | Full text | PDF (202K)


Focus on RNA interference - A user's guide
Tuning silence: conditional systems for RNA interference pp682 - 688
Maciej Wiznerowicz, Jolanta Szulc & Didier Trono
Published online: 23 August 2006 | doi:10.1038/nmeth914
Abstract | Full text | PDF (345K)


Focus on RNA interference - A user's guide
Expressing short hairpin RNAs in vivo pp689 - 695
Ola Snøve Jr & John J Rossi
Published online: 23 August 2006 | doi:10.1038/nmeth927
Abstract | Full text | PDF (160K)


Focus on RNA interference - A user's guide
Enzymatically prepared RNAi libraries pp696 - 700
Frank Buchholz, Ralf Kittler, Mikolaj Slabicki & Mirko Theis
doi:10.1038/nmeth912
Abstract | Full text | PDF (165K)


Focus on RNA interference - A user's guide
shRNA libraries and their use in cancer genetics pp701 - 706
René Bernards, Thijn R Brummelkamp & Roderick L Beijersbergen
Published online: 23 August 2006 | doi:10.1038/nmeth921
Abstract | Full text | PDF (320K)


Focus on RNA interference - A user's guide
Lessons from Nature: microRNA-based shRNA libraries pp707 - 714
Kenneth Chang, Stephen J Elledge & Gregory J Hannon
Published online: 23 August 2006 | doi:10.1038/nmeth923
Abstract | Full text | PDF (222K) | Supplementary Information


Focus on RNA interference - A user's guide
Genome-scale loss-of-function screening with a lentiviral RNAi library pp715 - 719
David E Root, Nir Hacohen, William C Hahn, Eric S Lander & David M Sabatini
Published online: 23 August 2006 | doi:10.1038/nmeth924
Abstract | Full text | PDF (197K)