载体名称：pMDC30
载体图谱：
载体序列及特征：
酶切位点分析（dnaman）：
Restriction analysis on pMDC30
Methylation: dam-No dcm-No
Screened with 117 enzymes, 364 sites found
AatII GACGT/C 1: 10246
Acc65I G/GTACC 1: 2056
AccIII T/CCGGA 4: 1576, 9371, 9507, 10044
AclI AA/CGTT 2: 1487, 4688
AflII C/TTAAG 1: 219
AgeI A/CCGGT 2: 6257, 9927
AhaIII TTT/AAA 10: 1317, 1656, 2202, 2636, 2713, 6323, 7980, 8009, 8376, 8536
Alw44I G/TGCAC 5: 672, 6790, 7288, 9683, 9985
AlwNI CAGNNN/CTG 4: 800, 4503, 4571, 7390
ApaBI GCANNNNN/TGC 5: 5219, 6799, 8818, 9692, 9968
ApaI GGGCC/C 1: 2054
ApaLI G/TGCAC 5: 672, 6790, 7288, 9683, 9985
AscI GG/CGCGCC 1: 2035
Asp718I G/GTACC 1: 2056
AsuII TT/CGAA 1: 2326
BalI TGG/CCA 8: 584, 680, 1309, 3146, 4694, 4961, 5428, 6374
BamHI G/GATCC 5: 1119, 1822, 2065, 2077, 2398
BbeI GGCGC/C 1: 6552
BbvII GAAGACNN/ 9: 10465, 10792, 2170, 2844, 8012, 10468, 10693, 10795, 11020
BclI T/GATCA 1: 8691
BglI GCCNNNN/NGGC 10: 2578, 3018, 3404, 3638, 3826, 3838, 3847, 4700, 5113, 5425
Bpu1102I GC/TNAGC 1: 8084
Bsc91I GAAGACNN/ 9: 10465, 10792, 2170, 2844, 8012, 10468, 10693, 10795, 11020
BsiI C/TCGTG 5: 7147, 10497, 10824, 9686, 10165
BsmI GAATGCN/ 7: 2589, 4935, 1178, 1585, 5612, 7698, 8592
Bsp1407I T/GTACA 3: 326, 728, 2011
BspHI T/CATGA 1: 8234
BspMI ACCTGCNNNN/ 5: 457, 2417, 5617, 6011, 9952
BspMII T/CCGGA 4: 1576, 9371, 9507, 10044
BssHII G/CGCGC 4: 24, 48, 1078, 2035
BstD102I GAG/CGG 7: 3387, 6907, 11286, 287, 6473, 9520, 9878
BstXI CCANNNNN/NTGG 3: 276, 592, 11087
ClaI AT/CGAT 1: 5843
Csp45I TT/CGAA 1: 2326
CspI CG/GWCCG 1: 9858
DraI TTT/AAA 10: 1317, 1656, 2202, 2636, 2713, 6323, 7980, 8009, 8376, 8536
DraIII CACNNN/GTG 2: 9691, 9984
DrdI GACNNNN/NNGTC 6: 6669, 7082, 9386, 9767, 10742, 11069
EagI C/GGCCG 20: 282, 1892, 3081, 3309, 3313, 3639, 3743, 4117, 4193, 4654, 5171,
5417, 5520, 5667, 5740, 5856, 6461, 9325, 9895, 10060
Ecl136II GAG/CTC 1: 266
Eco31I GGTCTCN/ 2: 6166, 563
Eco52I C/GGCCG 20: 282, 1892, 3081, 3309, 3313, 3639, 3743, 4117, 4193, 4654,
5171, 5417, 5520, 5667, 5740, 5856, 6461, 9325, 9895, 10060
Eco56I G/CCGGC 16: 3083, 3257, 3307, 3311, 3338, 3837, 4195, 4512, 4652, 4741,
4987, 5208, 5415, 5419, 5564, 6545
Eco57I CTGAAGNNNNNNNNNNNNNNNN/ 4: 3875, 4784, 7522, 2613
EcoICRI GAG/CTC 1: 266
EcoNI CCTNN/NNNAGG 1: 6076
EcoRI G/AATTC 1: 1572
EcoRV GAT/ATC 2: 7811, 10435
EheI GGC/GCC 1: 6550
EspI GC/TNAGC 1: 8084
FseI GGCCGG/CC 3: 3315, 3841, 5419
HindIII A/AGCTT 1: 2423
KpnI GGTAC/C 1: 2060
Mlu113I CC/GCGG 2: 273, 8686
MscI TGG/CCA 8: 584, 680, 1309, 3146, 4694, 4961, 5428, 6374
MstI TGC/GCA 2: 2568, 5980
NaeI GCC/GGC 16: 3085, 3259, 3309, 3313, 3340, 3839, 4197, 4514, 4654, 4743,
4989, 5210, 5417, 5421, 5566, 6547
NarI GG/CGCC 1: 6549
NcoI C/CATGG 1: 1271
NdeI CA/TATG 3: 4308, 6797, 9817
NheI G/CTAGC 1: 5763
NotI GC/GGCCGC 5: 282, 1892, 3639, 5171, 6461
NsiI ATGCA/T 3: 136, 2254, 7700
PacI TTAAT/TAA 1: 304
PflMI CCANNNN/NTGG 4: 1347, 2372, 4571, 5435
PinAI A/CCGGT 2: 6257, 9927
PmeI GTTT/AAAC 1: 2636
PstI CTGCA/G 2: 454, 2414
PvuI CGAT/CG 4: 252, 2549, 9220, 9907
PvuII CAG/CTG 3: 1676, 2518, 11154
RleAI CCCACANNNNNNNNNNNN/ 2: 6569, 5425
SacI GAGCT/C 1: 268
SacII CCGC/GG 2: 275, 8688
SalI G/TCGAC 2: 444, 2404
SapI GCTCTTCN/ 2: 6858, 6383
ScaI AGT/ACT 5: 1159, 2228, 2791, 9045, 9299
SciI CTC/GAG 3: 2043, 9208, 10302
SfiI GGCCNNNN/NGGCC 5: 3404, 3838, 3847, 5113, 5425
SgrAI CR/CCGGYG 1: 6545
SmaI CCC/GGG 3: 705, 2062, 10285
SpeI A/CTAGT 1: 295
SphI GCATG/C 1: 2760
SplI C/GTACG 1: 5647
SrfI GCCC/GGGC 1: 705
SspI AAT/ATT 2: 1264, 7738
SstI GAGCT/C 1: 268
SstII CCGC/GG 2: 275, 8688
SunI C/GTACG 1: 5647
Tth111I GACN/NNGTC 4: 6721, 8848, 9764, 10208
VspI AT/TAAT 3: 9020, 11146, 11205
XbaI T/CTAGA 3: 289, 1125, 2071
XcmI CCANNNNN/NNNNTGG 3: 3167, 4322, 8340
XhoI C/TCGAG 3: 2041, 9206, 10300
XmaI C/CCGGG 3: 703, 2060, 10283
XmaIII C/GGCCG 20: 282, 1892, 3081, 3309, 3313, 3639, 3743, 4117, 4193, 4654,
5171, 5417, 5520, 5667, 5740, 5856, 6461, 9325, 9895, 10060
XmnI GAANN/NNTTC 3: 9021, 10475, 10802
XorII CGAT/CG 4: 252, 2549, 9220, 9907
List by Site Order
24 BssHII 2423 HindIII 4987 Eco56I 8012 Bsc91I
48 BssHII 2518 PvuII 4989 NaeI 8084 Bpu1102I
136 NsiI 2549 XorII 5113 BglI 8084 EspI
219 AflII 2549 PvuI 5113 SfiI 8234 BspHI
252 PvuI 2568 MstI 5171 NotI 8340 XcmI
252 XorII 2578 BglI 5171 EagI 8376 DraI
266 EcoICRI 2589 BsmI 5171 Eco52I 8376 AhaIII
266 Ecl136II 2613 Eco57I 5171 XmaIII 8536 DraI
268 SacI 2636 DraI 5208 Eco56I 8536 AhaIII
268 SstI 2636 PmeI 5210 NaeI 8592 BsmI
273 Mlu113I 2636 AhaIII 5219 ApaBI 8686 Mlu113I
275 SacII 2713 AhaIII 5415 Eco56I 8688 SstII
275 SstII 2713 DraI 5417 Eco52I 8688 SacII
276 BstXI 2760 SphI 5417 XmaIII 8691 BclI
282 NotI 2791 ScaI 5417 EagI 8818 ApaBI
282 EagI 2844 Bsc91I 5417 NaeI 8848 Tth111I
282 XmaIII 2844 BbvII 5419 FseI 9020 VspI
282 Eco52I 3018 BglI 5419 Eco56I 9021 XmnI
287 BstD102I 3081 EagI 5421 NaeI 9045 ScaI
289 XbaI 3081 XmaIII 5425 SfiI 9206 XhoI
295 SpeI 3081 Eco52I 5425 BglI 9208 SciI
304 PacI 3083 Eco56I 5425 RleAI 9220 XorII
326 Bsp1407I 3085 NaeI 5428 MscI 9220 PvuI
444 SalI 3146 BalI 5428 BalI 9299 ScaI
454 PstI 3146 MscI 5435 PflMI 9325 EagI
457 BspMI 3167 XcmI 5520 EagI 9325 Eco52I
563 Eco31I 3257 Eco56I 5520 XmaIII 9325 XmaIII
584 MscI 3259 NaeI 5520 Eco52I 9371 BspMII
584 BalI 3307 Eco56I 5564 Eco56I 9371 AccIII
592 BstXI 3309 XmaIII 5566 NaeI 9386 DrdI
672 Alw44I 3309 EagI 5612 BsmI 9507 BspMII
672 ApaLI 3309 NaeI 5617 BspMI 9507 AccIII
680 BalI 3309 Eco52I 5647 SunI 9520 BstD102I
680 MscI 3311 Eco56I 5647 SplI 9683 ApaLI
703 XmaI 3313 NaeI 5667 Eco52I 9683 Alw44I
705 SmaI 3313 EagI 5667 XmaIII 9686 BsiI
705 SrfI 3313 Eco52I 5667 EagI 9691 DraIII
728 Bsp1407I 3313 XmaIII 5740 XmaIII 9692 ApaBI
800 AlwNI 3315 FseI 5740 Eco52I 9764 Tth111I
1078 BssHII 3338 Eco56I 5740 EagI 9767 DrdI
1119 BamHI 3340 NaeI 5763 NheI 9817 NdeI
1125 XbaI 3387 BstD102I 5843 ClaI 9858 CspI
1159 ScaI 3404 SfiI 5856 Eco52I 9878 BstD102I
1178 BsmI 3404 BglI 5856 XmaIII 9895 XmaIII
1264 SspI 3638 BglI 5856 EagI 9895 Eco52I
1271 NcoI 3639 NotI 5980 MstI 9895 EagI
1309 BalI 3639 EagI 6011 BspMI 9907 PvuI
1309 MscI 3639 XmaIII 6076 EcoNI 9907 XorII
1317 DraI 3639 Eco52I 6166 Eco31I 9927 PinAI
1317 AhaIII 3743 XmaIII 6257 AgeI 9927 AgeI
1347 PflMI 3743 Eco52I 6257 PinAI 9952 BspMI
1487 AclI 3743 EagI 6323 DraI 9968 ApaBI
1572 EcoRI 3826 BglI 6323 AhaIII 9984 DraIII
1576 BspMII 3837 Eco56I 6374 MscI 9985 ApaLI
1576 AccIII 3838 SfiI 6374 BalI 9985 Alw44I
1585 BsmI 3838 BglI 6383 SapI 10044 BspMII
1656 DraI 3839 NaeI 6461 NotI 10044 AccIII
1656 AhaIII 3841 FseI 6461 XmaIII 10060 XmaIII
1676 PvuII 3847 SfiI 6461 Eco52I 10060 Eco52I
1822 BamHI 3847 BglI 6461 EagI 10060 EagI
1892 EagI 3875 Eco57I 6473 BstD102I 10165 BsiI
1892 NotI 4117 EagI 6545 Eco56I 10208 Tth111I
1892 XmaIII 4117 Eco52I 6545 SgrAI 10246 AatII
1892 Eco52I 4117 XmaIII 6547 NaeI 10283 XmaI
2011 Bsp1407I 4193 EagI 6549 NarI 10285 SmaI
2035 AscI 4193 XmaIII 6550 EheI 10300 XhoI
2035 BssHII 4193 Eco52I 6552 BbeI 10302 SciI
2041 XhoI 4195 Eco56I 6569 RleAI 10435 EcoRV
2043 SciI 4197 NaeI 6669 DrdI 10465 BbvII
2054 ApaI 4308 NdeI 6721 Tth111I 10465 Bsc91I
2056 Acc65I 4322 XcmI 6790 ApaLI 10468 Bsc91I
2056 Asp718I 4503 AlwNI 6790 Alw44I 10468 BbvII
2060 KpnI 4512 Eco56I 6797 NdeI 10475 XmnI
2060 XmaI 4514 NaeI 6799 ApaBI 10497 BsiI
2062 SmaI 4571 AlwNI 6858 SapI 10693 Bsc91I
2065 BamHI 4571 PflMI 6907 BstD102I 10693 BbvII
2071 XbaI 4652 Eco56I 7082 DrdI 10742 DrdI
2077 BamHI 4654 XmaIII 7147 BsiI 10792 BbvII
2170 Bsc91I 4654 Eco52I 7288 Alw44I 10792 Bsc91I
2170 BbvII 4654 EagI 7288 ApaLI 10795 BbvII
2202 AhaIII 4654 NaeI 7390 AlwNI 10795 Bsc91I
2202 DraI 4688 AclI 7522 Eco57I 10802 XmnI
2228 ScaI 4694 BalI 7698 BsmI 10824 BsiI
2254 NsiI 4694 MscI 7700 NsiI 11020 BbvII
2326 AsuII 4700 BglI 7738 SspI 11020 Bsc91I
2326 Csp45I 4741 Eco56I 7811 EcoRV 11069 DrdI
2372 PflMI 4743 NaeI 7980 DraI 11087 BstXI
2398 BamHI 4784 Eco57I 7980 AhaIII 11146 VspI
2404 SalI 4935 BsmI 8009 DraI 11154 PvuII
2414 PstI 4961 BalI 8009 AhaIII 11205 VspI
2417 BspMI 4961 MscI 8012 BbvII 11286 BstD102I
Non Cut Enzymes
AvrII BglII BstEII Bsu36I CvnI Eam1105I
Eco47III Eco72I HpaI I-PpoI MfeI MluI
MstII NruI PmaCI SauI SnaBI SpoI
StuI SwaI
