Rosetta-gami B(DE3)pLysS

菌株名称：Rosetta-gami B(DE3)pLysS 菌株抗性：CamR, KanR, TetR 网站链接：http://www.merckbiosciences.com/ ... sp?catno=71137& 附件信息：The Rosetta-gami™ B series is the latest addition to Novagen's extensive line of E. coli strains designed for protein expression. These strains combine the key features of BL21 (and its Tuner™ derivative), Origami™, and Rosetta™ strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. All four Rosetta-gami B strains supply six different tRNAs to enable efficient translation of foreign transcripts that would otherwise be limited by the codon usage of E. coli. Rosetta-gami B strains also carry mutations in thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhances the formation of disulfide bonds in the cytoplasm. Deletion of the lac permease gene (lacY) allows adjustable, inducer (IPTG) dependent and uniform expression of target protein throughout all cells in a culture. Deficiencies in the lon and ompT proteases help to reduce proteolytic degradation. Due to various selection requirements, these strains are not compatible with tetracycline, kanamycin or chloramphenicol resistant plasmids. Rosetta-gami B hosts are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, because the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. The pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction and thus stabilize pET recombinants encoding target proteins that affect cell growth and viability. 该资源由“lindameng”酷友共享，特此感谢！